LOCUS       NM_177041               2241 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus flavin adenine dinucleotide synthetase 1 (Flad1),
            transcript variant 1, mRNA.
ACCESSION   NM_177041
VERSION     NM_177041.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Hirano,A., Braas,D., Fu,Y.H. and Ptacek,L.J.
  TITLE     FAD Regulates CRYPTOCHROME Protein Stability and Circadian Clock in
            Mice
  JOURNAL   Cell Rep 19 (2), 255-266 (2017)
   PUBMED   28402850
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Brizio,C., Galluccio,M., Wait,R., Torchetti,E.M., Bafunno,V.,
            Accardi,R., Gianazza,E., Indiveri,C. and Barile,M.
  TITLE     Over-expression in Escherichia coli and characterization of two
            recombinant isoforms of human FAD synthetase
  JOURNAL   Biochem Biophys Res Commun 344 (3), 1008-1016 (2006)
   PUBMED   16643857
REFERENCE   3  (bases 1 to 2241)
  AUTHORS   DELUCA,C. and KAPLAN,N.O.
  TITLE     Flavin adenine dinucleotide synthesis in animal tissues
  JOURNAL   Biochim Biophys Acta 30 (1), 6-11 (1958)
   PUBMED   13584389
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY240597.1 and BC046769.1.
            
            On Apr 13, 2018 this sequence version replaced NM_177041.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046769.1, BC025817.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                BY240597.1         1-18
            19-2241             BC046769.1         1-2223
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 39.1 cM"
     gene            1..2241
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /note="flavin adenine dinucleotide synthetase 1"
                     /db_xref="GeneID:319945"
                     /db_xref="MGI:MGI:2443030"
     exon            1..98
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     exon            99..321
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     CDS             235..1713
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /EC_number="2.7.7.2"
                     /note="FAD synthetase; FAD pyrophosphorylase; FMN
                     adenylyltransferase; flavin adenine dinucleotide synthase;
                     RFad1, flavin adenine dinucleotide synthetase, homolog"
                     /codon_start=1
                     /product="FAD synthase"
                     /protein_id="NP_796015.2"
                     /db_xref="CCDS:CCDS38492.1"
                     /db_xref="GeneID:319945"
                     /db_xref="MGI:MGI:2443030"
                     /translation="MASRASELPPGSGRSVTAGIIIVGDEILKGHTQDTNTYFLCRTL
                     RSLGVQVCRVSVVPDEVATIASEVNSFSRRFTHVLTAGGIGPTHDDVTFEAVAQAFGE
                     ELKPHPELQAAIKTLGGEGWEKLSMVPSSARLHYGTDPRTGHPFRFPLVSVRNVYLFP
                     GIPELLRRVLEGLKGLFQNTAVQFHLKELYVAASEGSIAPILSEAQAHFGRRLSLGSY
                     PDWSSNYFQVKLILDSEEKEPLEECLAYLTARLPQGSLVPYQPDAVEKAGEAVYKLAE
                     SGSCLGKKVAGALQTIETALAQYHLSQLCVGFNGGKDCTALLHLFHAAVQRKFPDVPK
                     PLQILYIRSISPFPELEQFLQDTIKRYNLQVLEAEGNMKQALGELQEKHPQLEAVLMG
                     TRRTDPYSCSLSHFSPTDPGWPSFMRINPLLDWTYRNIWEFLRQLFVPYCILYDRGYT
                     SLGSRENTTQNPALKCLSPGGHPVYRPAYLLENEDEERNSRM"
     exon            322..1066
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1214
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1313
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1503
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1577
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..2206
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1775..1780
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /note="hexamer: AGTAAA"
     polyA_site      1797
                     /gene="Flad1"
                     /gene_synonym="A930017E24Rik; Pp591"
                     /note="major polyA site"
ORIGIN      
        1 gaagctgtcc taggacatgc ggaaaggaaa gagcgaatga ccctgtgcag ggggcaaaat
       61 agaggtggga tgatacgaga tcagtgatct gaagcctggt tccctcaacc ccggaactca
      121 gggtatggcc tcctgcatcc tgtagacctg gcggcctctc agtacatacg gccccaattt
      181 ggggttctga gtggctactg gaaagccttg caaaagagca cagagggcag gaccatggca
      241 tctagggcct ctgaactgcc cccaggatca gggcgcagcg tgacagcagg catcatcatt
      301 gttggagatg agatccttaa gggacacacg caagatacca acacttactt tctttgccgg
      361 accctgcgct ccctgggggt ccaggtttgc cgagtctctg ttgtgcctga tgaagtagcc
      421 accattgcat ccgaggtcaa ctcattctcc aggcgcttca ctcacgtcct caccgcgggg
      481 ggcatcggcc ccactcatga tgatgttacc tttgaggcag tggcacaggc ttttggggag
      541 gagctcaaac cacaccctga gctccaagca gccatcaaaa ccctaggagg ggagggttgg
      601 gagaagctgt cgatggtgcc ctcctctgcc cgcctgcatt acggtacaga tcctcgaacc
      661 ggccacccct tcagattccc gctggtctct gtccgaaatg tctacctctt cccaggaatt
      721 ccggagcttc tgcgacgagt tttggaggga ctgaaagggc tgttccaaaa cacagctgtg
      781 cagttccacc tgaaggagtt gtatgtggcc gcgtcagagg gctctatcgc ccccatcctg
      841 tcggaggctc aggcccactt tgggcgtagg cttagcctgg gttcctaccc tgactggagc
      901 agcaactact ttcaagtgaa gctgattcta gactccgagg aaaaagagcc cttggaggaa
      961 tgtctggcct atctgactgc acgtctgccc caaggatcac tggtccccta ccagcccgat
     1021 gctgttgaga aggctggtga ggctgtgtac aaactcgcgg agtcaggctc ttgtctgggg
     1081 aagaaggtag caggtgccct acagaccatc gagactgccc tggctcagta ccatctctcc
     1141 cagctctgcg tgggcttcaa tgggggcaag gactgcaccg cactcctgca cctcttccat
     1201 gcagccgtgc agagaaaatt tcctgatgtt ccaaaacctc tccagatcct gtacattcgt
     1261 agcatctcgc ccttccctga gttggaacag tttctgcagg acaccatcaa gaggtataat
     1321 ctccaggtgt tagaagccga gggtaacatg aagcaggccc tgggtgagct tcaggaaaag
     1381 cacccccagc tggaggctgt cctgatgggc actcgacgga ctgaccccta ctcctgtagc
     1441 ctcagccatt tcagtcccac cgaccctggc tggccttcat tcatgagaat caacccgctg
     1501 ctggactgga cctacagaaa catctgggag tttctgcggc agctgtttgt cccatactgc
     1561 atcctatatg acagagggta cacatccctg gggagtcggg aaaacacaac gcagaatccg
     1621 gccctgaagt gcctgagccc aggaggacac cctgtctacc gtccagcata cctactggag
     1681 aatgaggatg aggagagaaa ttccagaatg tgatctcaca caagctgggg acaagacaga
     1741 gggaaggggg ctggcctggg ctacctgtca tgtcagtaaa gtgtacctca caccccattg
     1801 ttggtgcctg ttgctctggc tgtgccttcc tggtccgagg tcaagggagg ctgacagatc
     1861 tgaactggca gtgctccctc gggggtccag gagtaacata gaacatacta gaaaggagct
     1921 gctccagatg ggtctcagtg tcaaccacag ccagagccca ccaaaggaga ggaggcaacc
     1981 aggcagagca ctgcctgcat ggacgggaag ttgagctttc ctggctgtgg aggacagagg
     2041 atggagcagc caagcccgcc tgcccgccct aaccaccatt cctgtctgaa aggctgagtc
     2101 agcctgctca gaggcccaga accttctttt gctagaccct agagcctttg tttttagtca
     2161 tgaggcctga aggggtgggg acagtgaata aaaggtccca aaggcaaaaa aaaaaaaaaa
     2221 aaaaaaaaaa aaaaaaaaaa a
//
