LOCUS       NM_173397               1643 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus fat storage-inducing transmembrane protein 2 (Fitm2),
            mRNA.
ACCESSION   NM_173397
VERSION     NM_173397.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Bond,L.M., Ibrahim,A., Lai,Z.W., Walzem,R.L., Bronson,R.T.,
            Ilkayeva,O.R., Walther,T.C. and Farese,R.V. Jr.
  TITLE     Fitm2 is required for ER homeostasis and normal function of murine
            liver
  JOURNAL   J Biol Chem 299 (3), 103022 (2023)
   PUBMED   36805337
  REMARK    GeneRIF: Fitm2 is required for ER homeostasis and normal function
            of murine liver.
REFERENCE   3  (bases 1 to 1643)
  AUTHORS   Zheng,X., Ho,Q.W.C., Chua,M., Stelmashenko,O., Yeo,X.Y.,
            Muralidharan,S., Torta,F., Chew,E.G.Y., Lian,M.M., Foo,J.N.,
            Jung,S., Wong,S.H., Tan,N.S., Tong,N., Rutter,G.A., Wenk,M.R.,
            Silver,D.L., Berggren,P.O. and Ali,Y.
  TITLE     Destabilization of beta Cell FIT2 by saturated fatty acids alter
            lipid droplet numbers and contribute to ER stress and diabetes
  JOURNAL   Proc Natl Acad Sci U S A 119 (11), e2113074119 (2022)
   PUBMED   35254894
  REMARK    GeneRIF: Destabilization of beta Cell FIT2 by saturated fatty acids
            alter lipid droplet numbers and contribute to ER stress and
            diabetes.
REFERENCE   4  (bases 1 to 1643)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1643)
  AUTHORS   Nishihama,N., Nagayama,T., Makino,S. and Koishi,R.
  TITLE     Mice lacking fat storage-inducing transmembrane protein 2 show
            improved profiles upon pressure overload-induced heart failure
  JOURNAL   Heliyon 5 (3), e01292 (2019)
   PUBMED   30923760
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1643)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 1643)
  AUTHORS   Miranda,D.A., Koves,T.R., Gross,D.A., Chadt,A., Al-Hasani,H.,
            Cline,G.W., Schwartz,G.J., Muoio,D.M. and Silver,D.L.
  TITLE     Re-patterning of skeletal muscle energy metabolism by fat
            storage-inducing transmembrane protein 2
  JOURNAL   J Biol Chem 286 (49), 42188-42199 (2011)
   PUBMED   22002063
  REMARK    GeneRIF: FIT2 expression in skeletal muscle plays an unexpected
            function in regulating muscle energy metabolism and indicates an
            important role for lipid droplet formation in this process
REFERENCE   8  (bases 1 to 1643)
  AUTHORS   Gross,D.A., Zhan,C. and Silver,D.L.
  TITLE     Direct binding of triglyceride to fat storage-inducing
            transmembrane proteins 1 and 2 is important for lipid droplet
            formation
  JOURNAL   Proc Natl Acad Sci U S A 108 (49), 19581-19586 (2011)
   PUBMED   22106267
  REMARK    GeneRIF: Findings indicate that lipid droplet (LD)produced by
            storage-inducing transmembrane proteins 2 (FIT2) were approximately
            80% larger than storage-inducing transmembrane proteins 1 (FIT1).
REFERENCE   9  (bases 1 to 1643)
  AUTHORS   Gross,D.A., Snapp,E.L. and Silver,D.L.
  TITLE     Structural insights into triglyceride storage mediated by fat
            storage-inducing transmembrane (FIT) protein 2
  JOURNAL   PLoS One 5 (5), e10796 (2010)
   PUBMED   20520733
  REMARK    GeneRIF: FIT2 is a 6 transmembrane domain-containing protein whose
            conformation likely regulates its activity in mediating lipid
            droplet formation.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 1643)
  AUTHORS   Kadereit,B., Kumar,P., Wang,W.J., Miranda,D., Snapp,E.L.,
            Severina,N., Torregroza,I., Evans,T. and Silver,D.L.
  TITLE     Evolutionarily conserved gene family important for fat storage
  JOURNAL   Proc Natl Acad Sci U S A 105 (1), 94-99 (2008)
   PUBMED   18160536
  REMARK    GeneRIF: FIT1 and FIT2 are endoplasmic reticulum resident membrane
            proteins that induce lipid droplet accumulation in cell culture and
            when expressed in mouse liver.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK043244.1.
            
            On Jun 3, 2007 this sequence version replaced NM_173397.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK043244.1, AK054016.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1643              AK043244.1         2-1644
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 84.29 cM"
     gene            1..1643
                     /gene="Fitm2"
                     /gene_synonym="D930001I22Rik; Fit2"
                     /note="fat storage-inducing transmembrane protein 2"
                     /db_xref="GeneID:228859"
                     /db_xref="MGI:MGI:2444508"
     exon            1..227
                     /gene="Fitm2"
                     /gene_synonym="D930001I22Rik; Fit2"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..843
                     /gene="Fitm2"
                     /gene_synonym="D930001I22Rik; Fit2"
                     /note="fat-inducing transcript 2; fat-inducing protein 2"
                     /codon_start=1
                     /product="acyl-coenzyme A diphosphatase FITM2"
                     /protein_id="NP_775573.1"
                     /db_xref="CCDS:CCDS38315.1"
                     /db_xref="GeneID:228859"
                     /db_xref="MGI:MGI:2444508"
                     /translation="MEHLERCAWFLRGTLVRATVRRHLPWALVAAMLAGSVVKELSPL
                     PESYLSNKRNVLNVYFVKLAWAWTVCLLLPFIALTNYHLTGKTSLVLRRLSTLLVGTA
                     IWYICTALFSNIEHYTGSCYQSPALEGIRQEHRSKQQCHREGGFWHGFDISGHSFLLT
                     FCALMIVEEMAVLHEVKTDRGHHLHAAITTLVVALGFLTFIWVWMFLCTAVYFHDLTQ
                     KVFGTMFGLLGWYGTYGYWYLKSFSPGLPPQSCSLTLKRDTYKK"
     exon            228..1643
                     /gene="Fitm2"
                     /gene_synonym="D930001I22Rik; Fit2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1517..1522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fitm2"
                     /gene_synonym="D930001I22Rik; Fit2"
                     /note="hexamer: ATTAAA"
     polyA_site      1537
                     /gene="Fitm2"
                     /gene_synonym="D930001I22Rik; Fit2"
                     /note="major polyA site"
ORIGIN      
        1 aagacggtgg ccgagaaggc tctggaaggc ggacggtagc cgagaaggac cggcatggag
       61 cacctggagc gctgcgcgtg gtttctccgc gggacgctgg tgagggcgac ggtgcggcgc
      121 cacctgccct gggcgctggt ggccgccatg ctggcgggct cggtcgtcaa ggagctgtcc
      181 ccgctgcccg agagctacct cagcaacaag cgcaacgtcc tcaacgtgta ttttgttaaa
      241 ttggcctggg cctggacagt ctgtctcctc ctgcctttca ttgcccttac caactaccac
      301 ctgacgggca agaccagcct ggtcctgcgg cggttgagca ccctcctcgt gggcacagcc
      361 atctggtata tctgcacagc cctcttctcc aacattgagc actatacagg cagctgctac
      421 cagtccccgg ccctggaggg catcagacag gagcatcgca gcaagcagca gtgccaccgg
      481 gaagggggct tctggcatgg ctttgacatc tcgggccact ccttcctctt gaccttctgt
      541 gcgctcatga ttgtggaaga gatggccgtg ctgcatgagg tgaagacgga ccgcggccac
      601 cacctccatg ccgccatcac caccctggtt gtcgccctgg gtttcctgac cttcatctgg
      661 gtgtggatgt ttctgtgcac agccgtttat ttccacgact tgacccagaa agtgttcggc
      721 accatgtttg gtttgttggg ctggtacgga acatatgggt attggtatct gaagtctttc
      781 tctccaggac ttcctcccca gagttgtagt ttgactttga agcgagatac ttacaagaaa
      841 tgaaagagaa ggacataggg acaggaggac aatggctaat aggattccca cgtactttgt
      901 ccagtacaga attatctgtc ccatttcaga gagggccctt actaggtacc tttggtgtgg
      961 ggggcgcttg gggaacaagc ttagttggca gactctgctg gcggtgccac catgttgcca
     1021 gcagtgctac tcctgtcaga ttcttctcat ctatggcaag atgtgacctt aatctgtccc
     1081 cagagggaga gctgaggctc agcagaggga gagcagcctg cctcatcgtg tgggtcaaga
     1141 gagaatcagg gcttcaggat tctctgctgt ggcctccccg gctctgcttc tctttagagt
     1201 ttagaggcca cagtaatgct gttcttctaa cagcttcccc atggccaact ctgagataag
     1261 cctttgtctc tggcaggctc tttgttaaat accccaatgc ctcaaggaca ctctctggct
     1321 gtagcatccg cgtcctctgt ggaatgttga catttcacct ggccatgatt cccaacaggg
     1381 gcccatgacc ccgaagtgcc tggatggttg ttgttgttgt ttctctgtgt agccctgcgt
     1441 gttctggaac tcactctgta gaccaggctg gcctcagact cagagatctg cctgcctctg
     1501 cctcccgagt gctgggatta aagctgtgtc caccacggcc tggcctggca cttggatggt
     1561 ttctttttct gtccacacta tgtatcgtta tgtcttgggg tttggagcag taacagagaa
     1621 ccccattttc tctcttctct cct
//
