LOCUS       NM_001379528            1148 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus four and a half LIM domains 5 (Fhl5), transcript
            variant 3, mRNA.
ACCESSION   NM_001379528
VERSION     NM_001379528.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Gustafson,E.A., Seymour,K.A., Sigrist,K., Rooij,D.G.D.E. and
            Freiman,R.N.
  TITLE     ZFP628 Is a TAF4b-Interacting Transcription Factor Required for
            Mouse Spermiogenesis
  JOURNAL   Mol Cell Biol 40 (7), e00228-19 (2020)
   PUBMED   31932482
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Yabuta,Y., Ohta,H., Abe,T., Kurimoto,K., Chuma,S. and Saitou,M.
  TITLE     TDRD5 is required for retrotransposon silencing, chromatoid body
            assembly, and spermiogenesis in mice
  JOURNAL   J Cell Biol 192 (5), 781-795 (2011)
   PUBMED   21383078
REFERENCE   3  (bases 1 to 1148)
  AUTHORS   Wu,H., Chen,Y., Miao,S., Zhang,C., Zong,S., Koide,S.S. and Wang,L.
  TITLE     Sperm associated antigen 8 (SPAG8), a novel regulator of activator
            of CREM in testis during spermatogenesis
  JOURNAL   FEBS Lett 584 (13), 2807-2815 (2010)
   PUBMED   20488182
  REMARK    GeneRIF: Results indicate that SPAG8 acts as a regulator of ACT and
            plays an important role in CREM-ACT-mediated gene transcription
            during spermatogenesis.
REFERENCE   4  (bases 1 to 1148)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   5  (bases 1 to 1148)
  AUTHORS   Lardenois,A., Chalmel,F., Demougin,P., Kotaja,N., Sassone-Corsi,P.
            and Primig,M.
  TITLE     Fhl5/Act, a CREM-binding transcriptional activator required for
            normal sperm maturation and morphology, is not essential for
            testicular gene expression
  JOURNAL   Reprod Biol Endocrinol 7, 133 (2009)
   PUBMED   19930692
  REMARK    GeneRIF: Fhl5 is not essential for testicular gene expression to a
            level detected by microarray-based measurements.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1148)
  AUTHORS   Kotaja,N., De Cesare,D., Macho,B., Monaco,L., Brancorsini,S.,
            Goossens,E., Tournaye,H., Gansmuller,A. and Sassone-Corsi,P.
  TITLE     Abnormal sperm in mice with targeted deletion of the act (activator
            of cAMP-responsive element modulator in testis) gene
  JOURNAL   Proc Natl Acad Sci U S A 101 (29), 10620-10625 (2004)
   PUBMED   15247423
  REMARK    GeneRIF: Results indicate that numerous postmeiotic genes under
            CREM (cAMP-responsive element modulator) control require the
            coactivator function of ACT (activator of CREM) in testis.
REFERENCE   7  (bases 1 to 1148)
  AUTHORS   Macho,B., Brancorsini,S., Fimia,G.M., Setou,M., Hirokawa,N. and
            Sassone-Corsi,P.
  TITLE     CREM-dependent transcription in male germ cells controlled by a
            kinesin
  JOURNAL   Science 298 (5602), 2388-2390 (2002)
   PUBMED   12493914
  REMARK    GeneRIF: ACT selectively associates with KIF17b, a kinesin highly
            expressed in male germ cells; The ACT-KIF17b interaction is
            restricted to specific stages of spermatogenesis and directly
            determines the intracellular localization of ACT
REFERENCE   8  (bases 1 to 1148)
  AUTHORS   Deng,W. and Lin,H.
  TITLE     miwi, a murine homolog of piwi, encodes a cytoplasmic protein
            essential for spermatogenesis
  JOURNAL   Dev Cell 2 (6), 819-830 (2002)
   PUBMED   12062093
REFERENCE   9  (bases 1 to 1148)
  AUTHORS   Palermo,I., Litrico,L., Emmanuele,G., Giuffrida,V.,
            Sassone-Corsi,P., De Cesare,D., Maria Fimia,G., D'Agata,R.,
            Calogero,A.E. and Travali,S.
  TITLE     Cloning and expression of activator of CREM in testis in human
            testicular tissue
  JOURNAL   Biochem Biophys Res Commun 283 (2), 406-411 (2001)
   PUBMED   11327716
REFERENCE   10 (bases 1 to 1148)
  AUTHORS   Fimia,G.M., De Cesare,D. and Sassone-Corsi,P.
  TITLE     CBP-independent activation of CREM and CREB by the LIM-only protein
            ACT
  JOURNAL   Nature 398 (6723), 165-169 (1999)
   PUBMED   10086359
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL935267.13 and AL772199.11.
            
            Transcript Variant: This variant (3) represents the longest
            transcript. All three variants encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189682.28058.1,
                                           SRR5189685.83786.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AL935267.13        26490-26634         c
            146-210             AL935267.13        25668-25732         c
            211-381             AL772199.11        224691-224861       c
            382-556             AL772199.11        223675-223849       c
            557-726             AL772199.11        221261-221430       c
            727-913             AL772199.11        217150-217336       c
            914-1148            AL772199.11        209981-210215       c
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 10.58 cM"
     gene            1..1148
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="four and a half LIM domains 5"
                     /db_xref="GeneID:57756"
                     /db_xref="MGI:MGI:1913192"
     exon            1..145
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            146..210
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            211..381
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..1077
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="LIM-only protein ACT; FHL-5; activator of
                     cAMP-responsive element modulator in testis; activator of
                     CREM in testis"
                     /codon_start=1
                     /product="four and a half LIM domains protein 5"
                     /protein_id="NP_001366457.1"
                     /db_xref="GeneID:57756"
                     /db_xref="MGI:MGI:1913192"
                     /translation="MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQC
                     KEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKC
                     FHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFA
                     HYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLY
                     AKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC
                     GSGADTDA"
     exon            382..556
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            557..726
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            727..913
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1148
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1126..1131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="hexamer: ACTAAA"
     polyA_site      1148
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="major polyA site"
ORIGIN      
        1 acactgagag tacagaaagc aggtaacaat cctgagttat tttcttcaaa gccaacacta
       61 ctcagagttc tcaaatttcc caagaaagaa ctgaagagtg gcaacaaaga acacttcatc
      121 cgctgctcta caaagaactc caaagatttc tcattttgat gaaactcatg gctgcagcac
      181 agtggcagat aaatggaatt gcctctctgg gataaaactg aaatgacaag tagtcaattt
      241 gattgtcaat actgcacttc atccctgatt gggaagaaat atgtactcaa ggatgataat
      301 ctatactgca tctcctgcta cgatcgtatc ttttctaact attgtgagca gtgtaaagaa
      361 ccaattgaat cagattctaa ggatctttgc tacaaaaacc gtcactggca tgaaggatgc
      421 ttcaggtgca acaaatgcca tcactctttg gtggaaaagc ctttcgttgc caaggatgat
      481 cgcctgctgt gcacagactg ctattccaac gagtgttcct ccaagtgctt ccactgcaag
      541 agaaccatca tgccaggttc tcggaaaatg gaatttaagg gcaattactg gcatgaaacc
      601 tgctttgtgt gtgagcactg ccgacagcca ataggaacca agcctttgat ctccaaagag
      661 agtggcaatt attgtgtgcc atgttttgag aaggagtttg ctcattactg caacttctgt
      721 aagaaggtga taacttccgg tgggataacc ttccgtgatc agatatggca taaagagtgt
      781 tttctgtgca gcggctgcag gaaagagctt tatgaggagg catttatgtc aaaggatgat
      841 ttcccattct gcctggattg ctacaaccat ctttatgcta aaaagtgtgc agcctgcacc
      901 aaacccatca ctggcctcag aggtgccaag ttcatctgct ttcaagaccg ccagtggcac
      961 agtgagtgct tcaactgcgg aaagtgctcg gtctccttgg tgggtgaagg attcttgacc
     1021 cataacatgg aaatcttatg ccgcaagtgt ggctccgggg cagacactga cgcttagaaa
     1081 gagacaagtc tttcaccatc tgaaatctgc tttgcccttg ctctcactaa atctggaact
     1141 tagtggca
//
