LOCUS       NM_001355498            1057 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus four and a half LIM domains 5 (Fhl5), transcript
            variant 2, mRNA.
ACCESSION   NM_001355498 XM_011250080
VERSION     NM_001355498.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Gustafson,E.A., Seymour,K.A., Sigrist,K., Rooij,D.G.D.E. and
            Freiman,R.N.
  TITLE     ZFP628 Is a TAF4b-Interacting Transcription Factor Required for
            Mouse Spermiogenesis
  JOURNAL   Mol Cell Biol 40 (7), e00228-19 (2020)
   PUBMED   31932482
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Yabuta,Y., Ohta,H., Abe,T., Kurimoto,K., Chuma,S. and Saitou,M.
  TITLE     TDRD5 is required for retrotransposon silencing, chromatoid body
            assembly, and spermiogenesis in mice
  JOURNAL   J Cell Biol 192 (5), 781-795 (2011)
   PUBMED   21383078
REFERENCE   3  (bases 1 to 1057)
  AUTHORS   Wu,H., Chen,Y., Miao,S., Zhang,C., Zong,S., Koide,S.S. and Wang,L.
  TITLE     Sperm associated antigen 8 (SPAG8), a novel regulator of activator
            of CREM in testis during spermatogenesis
  JOURNAL   FEBS Lett 584 (13), 2807-2815 (2010)
   PUBMED   20488182
  REMARK    GeneRIF: Results indicate that SPAG8 acts as a regulator of ACT and
            plays an important role in CREM-ACT-mediated gene transcription
            during spermatogenesis.
REFERENCE   4  (bases 1 to 1057)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   5  (bases 1 to 1057)
  AUTHORS   Lardenois,A., Chalmel,F., Demougin,P., Kotaja,N., Sassone-Corsi,P.
            and Primig,M.
  TITLE     Fhl5/Act, a CREM-binding transcriptional activator required for
            normal sperm maturation and morphology, is not essential for
            testicular gene expression
  JOURNAL   Reprod Biol Endocrinol 7, 133 (2009)
   PUBMED   19930692
  REMARK    GeneRIF: Fhl5 is not essential for testicular gene expression to a
            level detected by microarray-based measurements.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1057)
  AUTHORS   Kotaja,N., De Cesare,D., Macho,B., Monaco,L., Brancorsini,S.,
            Goossens,E., Tournaye,H., Gansmuller,A. and Sassone-Corsi,P.
  TITLE     Abnormal sperm in mice with targeted deletion of the act (activator
            of cAMP-responsive element modulator in testis) gene
  JOURNAL   Proc Natl Acad Sci U S A 101 (29), 10620-10625 (2004)
   PUBMED   15247423
  REMARK    GeneRIF: Results indicate that numerous postmeiotic genes under
            CREM (cAMP-responsive element modulator) control require the
            coactivator function of ACT (activator of CREM) in testis.
REFERENCE   7  (bases 1 to 1057)
  AUTHORS   Macho,B., Brancorsini,S., Fimia,G.M., Setou,M., Hirokawa,N. and
            Sassone-Corsi,P.
  TITLE     CREM-dependent transcription in male germ cells controlled by a
            kinesin
  JOURNAL   Science 298 (5602), 2388-2390 (2002)
   PUBMED   12493914
  REMARK    GeneRIF: ACT selectively associates with KIF17b, a kinesin highly
            expressed in male germ cells; The ACT-KIF17b interaction is
            restricted to specific stages of spermatogenesis and directly
            determines the intracellular localization of ACT
REFERENCE   8  (bases 1 to 1057)
  AUTHORS   Deng,W. and Lin,H.
  TITLE     miwi, a murine homolog of piwi, encodes a cytoplasmic protein
            essential for spermatogenesis
  JOURNAL   Dev Cell 2 (6), 819-830 (2002)
   PUBMED   12062093
REFERENCE   9  (bases 1 to 1057)
  AUTHORS   Palermo,I., Litrico,L., Emmanuele,G., Giuffrida,V.,
            Sassone-Corsi,P., De Cesare,D., Maria Fimia,G., D'Agata,R.,
            Calogero,A.E. and Travali,S.
  TITLE     Cloning and expression of activator of CREM in testis in human
            testicular tissue
  JOURNAL   Biochem Biophys Res Commun 283 (2), 406-411 (2001)
   PUBMED   11327716
REFERENCE   10 (bases 1 to 1057)
  AUTHORS   Fimia,G.M., De Cesare,D. and Sassone-Corsi,P.
  TITLE     CBP-independent activation of CREM and CREB by the LIM-only protein
            ACT
  JOURNAL   Nature 398 (6723), 165-169 (1999)
   PUBMED   10086359
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL935267.13 and AL772199.11.
            
            On Apr 7, 2020 this sequence version replaced NM_001355498.1.
            
            Transcript Variant: This variant (2) encodes the same protein as
            variants 1 and 3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660819.192388.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               AL935267.13        25930-26048         c
            120-290             AL772199.11        224691-224861       c
            291-465             AL772199.11        223675-223849       c
            466-635             AL772199.11        221261-221430       c
            636-822             AL772199.11        217150-217336       c
            823-1057            AL772199.11        209981-210215       c
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 10.58 cM"
     gene            1..1057
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="four and a half LIM domains 5"
                     /db_xref="GeneID:57756"
                     /db_xref="MGI:MGI:1913192"
     exon            1..119
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            120..290
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..986
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="LIM-only protein ACT; FHL-5; activator of
                     cAMP-responsive element modulator in testis; activator of
                     CREM in testis"
                     /codon_start=1
                     /product="four and a half LIM domains protein 5"
                     /protein_id="NP_001342427.1"
                     /db_xref="CCDS:CCDS18009.1"
                     /db_xref="GeneID:57756"
                     /db_xref="MGI:MGI:1913192"
                     /translation="MTSSQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQC
                     KEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKC
                     FHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFA
                     HYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLY
                     AKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC
                     GSGADTDA"
     exon            291..465
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            466..635
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            636..822
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1057
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1035..1040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="hexamer: ACTAAA"
     polyA_site      1057
                     /gene="Fhl5"
                     /gene_synonym="1700027G07Rik; Act"
                     /note="major polyA site"
ORIGIN      
        1 ctcacataat gctgccgagc attctgttca tttggaagtc gacctgtctg tgattgagag
       61 ggaaatccac catctattct cccagcctgt aagtcaaaga aaaagcagct gaagaaaaag
      121 ataaaactga aatgacaagt agtcaatttg attgtcaata ctgcacttca tccctgattg
      181 ggaagaaata tgtactcaag gatgataatc tatactgcat ctcctgctac gatcgtatct
      241 tttctaacta ttgtgagcag tgtaaagaac caattgaatc agattctaag gatctttgct
      301 acaaaaaccg tcactggcat gaaggatgct tcaggtgcaa caaatgccat cactctttgg
      361 tggaaaagcc tttcgttgcc aaggatgatc gcctgctgtg cacagactgc tattccaacg
      421 agtgttcctc caagtgcttc cactgcaaga gaaccatcat gccaggttct cggaaaatgg
      481 aatttaaggg caattactgg catgaaacct gctttgtgtg tgagcactgc cgacagccaa
      541 taggaaccaa gcctttgatc tccaaagaga gtggcaatta ttgtgtgcca tgttttgaga
      601 aggagtttgc tcattactgc aacttctgta agaaggtgat aacttccggt gggataacct
      661 tccgtgatca gatatggcat aaagagtgtt ttctgtgcag cggctgcagg aaagagcttt
      721 atgaggaggc atttatgtca aaggatgatt tcccattctg cctggattgc tacaaccatc
      781 tttatgctaa aaagtgtgca gcctgcacca aacccatcac tggcctcaga ggtgccaagt
      841 tcatctgctt tcaagaccgc cagtggcaca gtgagtgctt caactgcgga aagtgctcgg
      901 tctccttggt gggtgaagga ttcttgaccc ataacatgga aatcttatgc cgcaagtgtg
      961 gctccggggc agacactgac gcttagaaag agacaagtct ttcaccatct gaaatctgct
     1021 ttgcccttgc tctcactaaa tctggaactt agtggca
//
