LOCUS       NM_001301817            8423 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus FYVE, RhoGEF and PH domain containing 4 (Fgd4),
            transcript variant 1, mRNA.
ACCESSION   NM_001301817 XM_006522021
VERSION     NM_001301817.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Song,X.Y., Chen,Y.Y., Liu,W.T., Cong,L., Zhang,J.L., Zhang,Y. and
            Zhang,Y.Y.
  TITLE     Atorvastatin reduces IOP in ocular hypertension in vivo and
            suppresses ECM in trabecular meshwork perhaps via FGD4
  JOURNAL   Int J Mol Med 49 (6) (2022)
   PUBMED   35417030
  REMARK    GeneRIF: Atorvastatin reduces IOP in ocular hypertension in vivo
            and suppresses ECM in trabecular meshwork perhaps via FGD4.
REFERENCE   3  (bases 1 to 8423)
  AUTHORS   van der Weyden,L., Swiatkowska,A., Iyer,V., Speak,A.O. and
            Adams,D.J.
  TITLE     A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators
            of Pulmonary Metastatic Colonisation
  JOURNAL   G3 (Bethesda) 10 (6), 1869-1877 (2020)
   PUBMED   32245826
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8423)
  AUTHORS   Ko,H.K., Guo,L.W., Su,B., Gao,L. and Gelman,I.H.
  TITLE     Suppression of chemotaxis by SSeCKS via scaffolding of
            phosphoinositol phosphates and the recruitment of the Cdc42 GEF,
            Frabin, to the leading edge
  JOURNAL   PLoS One 9 (10), e111534 (2014)
   PUBMED   25356636
  REMARK    GeneRIF: The enrichment of activated Cdc42 in SSeCKS-null leading
            edge filopodia correlated with recruitment of the Cdc42-specific
            guanine nucleotide exchange factor, Frabin.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8423)
  AUTHORS   Horn,M., Baumann,R., Pereira,J.A., Sidiropoulos,P.N., Somandin,C.,
            Welzl,H., Stendel,C., Luhmann,T., Wessig,C., Toyka,K.V.,
            Relvas,J.B., Senderek,J. and Suter,U.
  TITLE     Myelin is dependent on the Charcot-Marie-Tooth Type 4H disease
            culprit protein FRABIN/FGD4 in Schwann cells
  JOURNAL   Brain 135 (Pt 12), 3567-3583 (2012)
   PUBMED   23171661
  REMARK    GeneRIF: This study demonistrated that the disease-initiating event
            in CMT4H is loss of a Schwann cell-specific cellular function of
            Frabin/Fgd4.
REFERENCE   6  (bases 1 to 8423)
  AUTHORS   Hackney,J.A., Charbord,P., Brunk,B.P., Stoeckert,C.J.,
            Lemischka,I.R. and Moore,K.A.
  TITLE     A molecular profile of a hematopoietic stem cell niche
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13061-13066 (2002)
   PUBMED   12226475
REFERENCE   7  (bases 1 to 8423)
  AUTHORS   Kim,Y., Ikeda,W., Nakanishi,H., Tanaka,Y., Takekuni,K., Itoh,S.,
            Monden,M. and Takai,Y.
  TITLE     Association of frabin with specific actin and membrane structures
  JOURNAL   Genes Cells 7 (4), 413-420 (2002)
   PUBMED   11952837
REFERENCE   8  (bases 1 to 8423)
  AUTHORS   Ikeda,W., Nakanishi,H., Takekuni,K., Itoh,S. and Takai,Y.
  TITLE     Identification of splicing variants of Frabin with partly different
            functions and tissue distribution
  JOURNAL   Biochem Biophys Res Commun 286 (5), 1066-1072 (2001)
   PUBMED   11527409
REFERENCE   9  (bases 1 to 8423)
  AUTHORS   Ono,Y., Nakanishi,H., Nishimura,M., Kakizaki,M., Takahashi,K.,
            Miyahara,M., Satoh-Horikawa,K., Mandai,K. and Takai,Y.
  TITLE     Two actions of frabin: direct activation of Cdc42 and indirect
            activation of Rac
  JOURNAL   Oncogene 19 (27), 3050-3058 (2000)
   PUBMED   10871857
REFERENCE   10 (bases 1 to 8423)
  AUTHORS   Obaishi,H., Nakanishi,H., Mandai,K., Satoh,K., Satoh,A.,
            Takahashi,K., Miyahara,M., Nishioka,H., Takaishi,K. and Takai,Y.
  TITLE     Frabin, a novel FGD1-related actin filament-binding protein capable
            of changing cell shape and activating c-Jun N-terminal kinase
  JOURNAL   J Biol Chem 273 (30), 18697-18700 (1998)
   PUBMED   9668039
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CT476831.7, AC169505.1 and
            CT573014.9.
            
            On Dec 20, 2022 this sequence version replaced NM_001301817.1.
            
            Summary: This gene is a member of the FYVE, RhoGEF and PH domain
            containing (FGD) family. The encoded protein is a Cdc42-specific
            guanine nucleotide exchange factor (GEF) that plays an essential
            role in regulating the actin cytoskeleton and cell morphology.
            Disruption of the gene in mouse causes abnormal nerve development
            and dysmyelination. Mutations in a similar gene in human can cause
            Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the
            peripheral nervous system. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Sep 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC129903.1, SRR7345562.2412234.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               CT476831.7         81098-81359
            263-382             AC169505.1         8920-9039
            383-566             AC169505.1         22530-22713
            567-1074            AC169505.1         28539-29046
            1075-1164           AC169505.1         35623-35712
            1165-1310           AC169505.1         37975-38120
            1311-1467           AC169505.1         38753-38909
            1468-1606           AC169505.1         43294-43432
            1607-1665           AC169505.1         51077-51135
            1666-1812           AC169505.1         51396-51542
            1813-1985           AC169505.1         58972-59144
            1986-2016           CT573014.9         15606-15636
            2017-2109           CT573014.9         16021-16113
            2110-2235           CT573014.9         16580-16705
            2236-2376           CT573014.9         26739-26879
            2377-2517           CT573014.9         28590-28730
            2518-8423           CT573014.9         29826-35731
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.18 cM"
     gene            1..8423
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /note="FYVE, RhoGEF and PH domain containing 4"
                     /db_xref="GeneID:224014"
                     /db_xref="MGI:MGI:2183747"
     exon            1..262
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..2775
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Fgd1-related F-actin-binding protein; FYVE, RhoGEF and PH
                     domain-containing protein 4; actin filament-binding
                     protein frabin"
                     /codon_start=1
                     /product="FYVE, RhoGEF and PH domain-containing protein 4
                     isoform 1"
                     /protein_id="NP_001288746.1"
                     /db_xref="GeneID:224014"
                     /db_xref="MGI:MGI:2183747"
                     /translation="MNSESDHNFRRVAIRRKSGSYLPPGIPRPWSRPSPQLRRLDRVA
                     QPSQAAGINTCAKVTPPCTTSNTTTEAQGINGRTPAKRSTASAKPQVPPKPLYLQNSP
                     SSHIPQVPRHKALPSAKPRMEESNPAPTSCTSKGKHSKVSDLISHFEGGSVLSSYIDL
                     QKDSTMNLNIPQTLGQPGLTSSPPRKFLPQHSPQKQENDPDQTQGQHGCLANGVVAAQ
                     NQMECEDEKETTLSPEMAIQTAAASPDTHVLNGERNETITDSASSIANSHDENASDSS
                     CRTPGTDLGLPSKEGEPGMDAELQERENGVNTMGLDTLDQHHEVKETNEQKLHKIATE
                     LLLTERAYVSRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPE
                     LEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAVELVKTMTERVPQFKSVTEE
                     IQKQKICGSLTLQHHMLEPIQRIPRYEMLLKDYLKKLSPDSPDWNDAKKSLEIISTAA
                     SHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLF
                     LFNNMLLYCVPRFSLVGSKFTVRTRVGIDGMKIVETHNEEYPHTFQISGKERTLELQA
                     SSEQDKEEWIKALQESIDAFHQRHETFRNAIAKENDIPLEVSTAELGKRAPRWIRDNE
                     VTMCMKCKESFNALTRRRHHCRACGHVVCWKCSDYKAQLEYDGGRLNKVCKDCYQIIS
                     GFTDSEEKKRRGILEIESAEVSGNSEVCSFLQYMEKSKPWQKIWCVIPKQDPLVLYMY
                     GAPQDVRAQATIPLLGYVVDDMPKSADLPHSFKLTQSKSVHSFAADNEELKQKWLKII
                     LLAVTGETPDGPSEHLATLNNLPGPKKKSEC"
     exon            263..382
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            383..566
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            567..1074
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1164
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1310
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1467
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1606
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1665
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1812
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1985
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2016
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2109
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2235
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2376
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2517
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..8423
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3337..3342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /note="hexamer: AATAAA"
     polyA_site      3358
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /note="major polyA site"
     regulatory      3783..3788
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
                     /note="hexamer: ATTAAA"
     polyA_site      3818
                     /gene="Fgd4"
                     /gene_synonym="9030023J02Rik; 9330209B17Rik; Frabp;
                     ZFYVE6"
ORIGIN      
        1 ggagtcggag cgcaggcccg ggaagcgagg cctgcgcggg gagggagcgc agggagtcgc
       61 gcaacctgga cgcgcgcggg gcaaggcttg cgggagtgac cgcaggccac catgaacagc
      121 gagagcgacc ataacttcag gcgggtggcc atccggcgga agtcaggctc ctacctgccg
      181 cccgggatcc cccggccctg gagcaggcct tcgccgcaat tgcggcgcct tgaccgtgtc
      241 gcgcaaccca gccaggccgc aggcatcaat acttgtgcaa aggttactcc accttgtacg
      301 acaagcaaca caaccacaga agctcaggga ataaatggga ggacaccagc aaaacgctcc
      361 actgcaagtg caaagccaca agtgcctcca aaaccattat atctgcagaa ttcaccttca
      421 tcccatattc cccaagtccc caggcataaa gctttaccta gtgcaaagcc aaggatggag
      481 gagtctaatc cggcccctac ttcttgtacc tcaaaaggga agcatagtaa ggtatcggat
      541 ctcatcagcc actttgaagg aggcagtgtt ttgtcaagtt acattgattt gcagaaagat
      601 tctactatga acctcaatat tcctcaaact ctaggacagc ctgggttaac ctcctcacct
      661 ccacgaaaat ttctgcccca gcactctcca cagaagcagg aaaacgaccc agatcagact
      721 caggggcaac atggttgttt ggccaatggt gtagtggctg cacaaaacca gatggaatgt
      781 gaagatgaga aggagactac tcttagccca gagatggcta ttcagactgc tgccgcctct
      841 cctgatacac atgtgctgaa tggagaaaga aatgaaacca tcacagattc tgcatcatcc
      901 atagccaata gtcatgatga aaatgcttct gacagcagct gcaggactcc agggacggac
      961 ttagggcttc cctcaaaaga aggggagcca gggatggacg ctgagctcca agagagggaa
     1021 aatggggtga acaccatggg attggatacg ttggaccagc accatgaagt gaaggagact
     1081 aatgaacaga aacttcacaa aatagccact gaacttttac ttacagaaag agcttatgtc
     1141 agccggctcg acctcctaga tcaggtattt tattgcaaac tattagaaga agcaaaccga
     1201 ggctcatttc ctgcagagat ggtgaataaa atcttttcta acatttcatc aataaatgcc
     1261 ttccatagta aattcctatt acctgagctg gagaaacgaa tgcaagaatg ggaaactaca
     1321 cccagaattg gagatatcct gcaaaagttg gcgccattcc ttaagatgta tggagaatac
     1381 gtgaagggat ttgataatgc agtggaactg gttaaaacca tgacagagcg tgttccccag
     1441 tttaaatcag tgactgaaga gattcagaaa cagaagatct gtggaagctt aacgctgcag
     1501 catcacatgc tggagcctat tcagcgcatt cctcgctatg agatgctcct gaaggactac
     1561 ctgaagaagt tgtctcctga ctccccagac tggaatgatg caaaaaagtc acttgaaatt
     1621 atatctacag cagcaagcca ttctaatagt gcaataagaa aaatggagaa cctgaagaaa
     1681 cttttagaaa tttatgagat gttgggagaa gaagaggata ttgtaaatcc ctcaaatgaa
     1741 ctaataaaag aaggacaaat cctcaaacta gcagctcgga acacatcagc acaagagcgc
     1801 tacctcttct tattcaacaa catgttgcta tattgtgtgc ccagattcag cttggttggc
     1861 tcaaaattca cagttcgaac cagagttggc attgatggaa tgaaaattgt ggagactcac
     1921 aatgaagaat atccacacac tttccagata tctgggaaag aaagaaccct ggagctgcag
     1981 gccagttctg aacaagacaa ggaagaatgg atcaaggccc ttcaagaaag tattgatgct
     2041 tttcatcaaa ggcatgaaac tttcagaaat gcaatcgcaa aggaaaatga cattccccta
     2101 gaagtttcta ctgctgagct gggaaaacga gctccaagat ggatacgtga taatgaagtg
     2161 accatgtgta tgaagtgcaa agagtccttc aatgcactga ccagaaggcg gcatcactgc
     2221 cgggcatgtg gacatgtggt ttgttggaaa tgttctgact acaaagctca gcttgagtat
     2281 gatggtggaa gattgaacaa ggtttgtaaa gactgttacc agataataag tggattcaca
     2341 gacagtgaag aaaagaaaag aaggggcatt ttagagattg aatcagcaga agtatcagga
     2401 aatagtgagg tgtgcagttt ccttcagtac atggaaaagt caaagccttg gcagaaaatc
     2461 tggtgtgtga tccccaagca agaccccctt gtgctgtaca tgtatggcgc tcctcaggat
     2521 gtcagagccc aagcgaccat acccctcctg ggctacgttg tggatgatat gcctaagagt
     2581 gcagatctgc cacatagttt caagctgacc cagtccaagt ctgtgcatag ctttgctgca
     2641 gacaatgagg aactgaaaca gaaatggctg aaaatcatcc ttctagctgt cacaggtgag
     2701 acaccagatg gcccaagtga gcatctagcc accttgaaca atctccctgg acccaagaaa
     2761 aagtcagaat gctaaacttc agcagctgct agtgtggagt ttccagtaat acacatagct
     2821 caagacatcc ccagctctcc ttccttgtct cttagcactt tgcattgaga aatatggttt
     2881 cataaacaca tcttttgggg acttctttca tatgtttata tataataaaa tcagtatgca
     2941 gagccattct tctgttgaag ttctaaaata atgtggaaaa tggaaccttt ttgataagct
     3001 attccttaac tgtgtatgtt tgccttgagt gggttaaaaa atgttaactg tcgaatcact
     3061 acctggttat aatgggcctt tcatttaaga aatccttcat tgtcttgttc acatgtagaa
     3121 cttgtaagag tgtgaaaaat atacctccat gtaaccaaaa tagatctatg gtaaaaagta
     3181 cagggacagt ttagagtatt gtatgaactt gtctagtttg taagtctcat gctatgtaaa
     3241 tagtttctgt tcttatacaa cacaaaaaat tataggttcc tgatgttgat actttttgac
     3301 ttaatataat acttgttttg tagaattact cctaataata aaatatctaa aatacaaatc
     3361 aatttgcctg tatataatat tgaaaataag tatgaatatg tcagctttct cttcagcttt
     3421 agttttgttt tcactctggt taattcttta aatataaaac ctattttatt tctaaactta
     3481 gcagtgttct aaatttaaga aaaattggta agttatgttg aactgattag aatctaagtg
     3541 tattgatatt tttatagtaa aaaagagtaa tttatgtggt gtcagatata gtgtgaattt
     3601 caacagtatt tttagagtac tatttttctt ttgttctgta cctatttaaa acagtgcctc
     3661 tcttagaagg tgctattaat tcttatttag ataaaagatg agggtttagt aaatagtaac
     3721 taaacattat tatttttaac tactctgctt attctcaact taagtgcact ttggtcacag
     3781 agattaaaat gtatggctta ctttacattt tatattaaca taattgtcat ggtaatttaa
     3841 tgtcaacata tgcaaaaact tcctaaaact atataagaca tctgacctta tagagccctg
     3901 ggatatatgg aagacgctgg tttactgttt actgtcctcc tttccattgg actgttatgg
     3961 acgggtaaat gaggacaaca tctaataaac acaaggtggc tgggtgagct ttccttctgt
     4021 cagctacaaa gcggagctca tgcgtgtgcc agcttatctg ggagaagaga gttattagta
     4081 aaggaaattt atgcttaatc attcactaga aaagatagat gaaaccagga tttcagaatc
     4141 aataatggca gatttctatg agatgggaag cactaaagaa actgcagaat tctccctttt
     4201 tatcccatgg actctcagat gaactatggc tgtctattag gttgtgcttg tgactaaaat
     4261 cttatgttga gtttactgtt taagttaata cttttctcat attagacttt tttcctttct
     4321 tttccattct ccaaacatta ccacttttac tgtactgttt actaggaatc catagaatgt
     4381 cttcactaca cacaatcttt aagggctaca cttgagatgt agagtacaat ttatcagaaa
     4441 ttgagaccac catacattta tacacctcat gttgcacaag aaacgtctca ttttttttct
     4501 actccaaagg agcaagggaa ccaattaggt tgctttcctc catgaaatac cagacatgaa
     4561 cattaacatt aaaccatcta tttagaatca ctaatgggca aaacacaaag ctatctcctt
     4621 attccctact aaatgagagt catatagaaa gaaattgtac attgtttctc ttttctagtt
     4681 gtcagcattg atgctgtgtt tgagtgggag gaaagaaacc aggtgtgaag ggtacctatt
     4741 gataaaatta aacaataact atacatctta ctttattttt catatttcaa gaaaagaaaa
     4801 gcagtaaata tgtagaatga ctatcagaat aaggatgact tctgtaaata taagcagaat
     4861 aatgagtgaa ctgtctcact tagaagattt atgtcagata atatttaaaa aaaaaactat
     4921 ttgatcagca agaagagtac atgtttgcct accagcaaat tcttgaatag aaggaatatg
     4981 attagatatt cagtgtagtt caacttagat tattgaaagg atactcataa aaagaatgtt
     5041 agcatgcttc tagtgttcat tcagtaagga gtgtgagtgc cagaagagga tcttctctgg
     5101 ttctcacctt tacttctccc cacactgagc caagcacaaa ccactttccc catctctctc
     5161 aaaataacca caaatacttt tagtaggaga aaaaagtaat agaataatat tagttactta
     5221 ttaagaactc aactctgggg ctaaggagaa ttctatgcaa ggatgagaaa cagttcagac
     5281 aggtctccgg agaccacata aaggccaggc aagcaagggc aacagaggtg gggccaggaa
     5341 gttttggtga aggtgctcta tccaggcaag tcagagcagc gagtgcagga acagtaagta
     5401 accttgcctc aatgatgagg tagagaggca ccaaggaagt cacctgacat agcctcagat
     5461 ctctccacat ttgcacagtt ccctaaatat atgtgaacac acatgcatgg acagtgtgca
     5521 tgcacataca cattaaaaaa tctaattcaa ataaactcca atccaagagg ttgttagaat
     5581 aactaacctc tttttgagat aagtagggtt tcatgataaa ctgagcctga acatagtatc
     5641 agttcctttc ctgatcaaat aaatgtttca tatggttctt aatttgttat tttctctagc
     5701 ttagtgtgtt caaaagtaat ctttaagaaa aatgtccttc cttcaaattt aagaaaatat
     5761 ttctttggta aaattgttat tcattcaaag catttcaact ttcaacataa ttgttgcttt
     5821 tgttgaaagt ctaccatttg gaaaaggtga gatgtggtac atgcttttat ctaaactttt
     5881 atgtggagtt caataatgaa aaatgccact agccactttt gtaataatac caagcaataa
     5941 aactctccag tatgagttta taccaaaagt ttaacatgaa ggccctaagc tacagcatat
     6001 agagaatcaa cttctgagaa gtgatatgat agtccaaatt gctgagcaca ggggaaagaa
     6061 ggaaggcatc gttccatggc ttcaggcata tcaactgtgt ttaatttctt ttttttttag
     6121 atttatttat tttatgtata tgagtacact gtcactatct ttagacacac ccgaagaagg
     6181 catcagatcc cattgcagat ggttgtgaac caccatgcgg ttgctgggaa ttgaactcag
     6241 gacctcttaa ttgctgagcc atcactctag ccttgtgttt aatttctaca aaggctatgt
     6301 ataatgaagc agtttgtttg ctctgctctg ttttatcagc tgtgttgtac atttctgtga
     6361 attgaattca acataaaggt tgccatactg tcttcactgg attacctaca ccatttttta
     6421 aatataggaa ttaaccttgt aaatgttccc tgctgaattt tctaaacaag agtatatgtt
     6481 gccatcagaa ctgtttaact ataattccat ggtacttcag ggttgttgaa gagccactga
     6541 tgccttctaa aatcctctac tcaaaaatac ttctggagtt catgattctg ttttttgggg
     6601 tatttgggga gattgattgt tttcctcctt ttggttgagg tggaagggtt ttccctcctt
     6661 cagttatttt cagatatcat ttgtttattt ctaaagcttt aacttattca gagtgtattg
     6721 tctgaaatgg attcttaatc ctcatatctg tcagtgtgtt gtcatggcaa atgagcatga
     6781 ataagagtaa atcaattccc agaaagcaca ggtcttattt atctctgttg ttttaagagt
     6841 agaaggtaac ttagctttcc tacatcacat gataagcaca tggtatgtat aatctaaaat
     6901 ttgaaagaca gaacaatctt agtgttttat ttaatgttgt gatcttcaag aatgtatgaa
     6961 tataagaact gaggctaaag agttcctctt attagagtta cattcacatt tgtagtggat
     7021 cctgtcactt tttcattcta agtttaatta gaatcaagtt ttttattttc atataactat
     7081 caaactatag gttccctaga ttttaaaaga aaagcattag actactagat ctatctaaat
     7141 actctctgtg actttatatg tataaatgta caaattataa atcttcaatt ctcaccaaag
     7201 aacaaaatgt tatgtaaaat ttaatttaag tattttaaca aaattattca catgtgtttt
     7261 taatgttata attcatcttt acccaagccc atcagtcttg ctaggttaca ggaagaggca
     7321 atgttgcttt tgttgcaggt atagagagag cacctacctt agcacatgaa aggaatgaat
     7381 ggagctggat aaatgggctg actacttgta tccatgtgaa ctgctcgaga gaaataagtt
     7441 agaagatgcc tatgtacttg ttggtggatg aaatccttgt gctcaggatt tgaccttggc
     7501 atctaaatta tacatgatat tatcttatca ttactttatt tcttctttaa gcttttacaa
     7561 agtaatctca ttctataagc catttttctg ctatttttca ctctaaagtc aactttctaa
     7621 gagaagggaa tggctaaatg ctgtgtcttt tctgtatttc aagataaaat aacacatggc
     7681 ttctacaaga tagtttattt gttggaagtg ggggtcactg aaagttacat aaagatcata
     7741 ctttgcccta aaattcattg tgatcaattt gataaaaaaa attaaatcaa attttagtat
     7801 attttaataa gtgaatacta aatgcatcaa gtaaatacag accttatagc ccattatgat
     7861 gagaaaattg aattgtgtat tatgaaatat gaagactcaa atatatacta tatattattt
     7921 aacatttaat cattcatata taaaaccaaa ctgccatttc atattttaag agacaattaa
     7981 ttgttttgtt ttggttttgg ttttttggtt tttcgagaca aggtttctct gtatagctgt
     8041 cctggaactc actttgttga ccaggctggc ctcgaactca gaaatccacc tgcctctgcc
     8101 tcgagtgctg ggattaaagg tgtgtgccac cacacccagt gctaattgtt atttaaaaaa
     8161 ctaagaatta ttttgatttt tcttttgaca tgtttactga gtttcaaagg gcacatcaga
     8221 attttatagt tgtgaaaata taaacatccc tattttgcag tggtgtgaat gtacaatact
     8281 tagatatgct gtatatatag tgtaacatct gtatatttaa tcataaaggc agataattga
     8341 aaacattgta aaattaagca cttcaattct tgttaaatat gaaacatttg tatcttgtaa
     8401 ataaagacat ccttaaattg gtt
//
