LOCUS       NM_007998               2941 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus ferrochelatase (Fech), transcript variant 1, mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_007998 XM_001000060
VERSION     NM_007998.8
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Sardar Pasha,S.P.B., Shetty,T., Lambert-Cheatham,N.A., Sishtla,K.,
            Mathew,D., Muniyandi,A., Patwari,N., Bhatwadekar,A.D. and
            Corson,T.W.
  TITLE     Retinal Phenotyping of Ferrochelatase Mutant Mice Reveals
            Protoporphyrin Accumulation and Reduced Neovascular Response
  JOURNAL   Invest Ophthalmol Vis Sci 62 (2), 36 (2021)
   PUBMED   33620374
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Pran Babu,S.P.S., White,D. and Corson,T.W.
  TITLE     Ferrochelatase regulates retinal neovascularization
  JOURNAL   FASEB J 34 (9), 12419-12435 (2020)
   PUBMED   32716567
  REMARK    GeneRIF: Ferrochelatase regulates retinal neovascularization.
REFERENCE   3  (bases 1 to 2941)
  AUTHORS   Halloy,F., Iyer,P.S., Cwiek,P., Ghidini,A., Barman-Aksozen,J.,
            Wildner-Verhey van Wijk,N., Theocharides,A.P.A., Minder,E.I.,
            Schneider-Yin,X., Schumperli,D. and Hall,J.
  TITLE     Delivery of oligonucleotides to bone marrow to modulate
            ferrochelatase splicing in a mouse model of erythropoietic
            protoporphyria
  JOURNAL   Nucleic Acids Res 48 (9), 4658-4671 (2020)
   PUBMED   32313951
REFERENCE   4  (bases 1 to 2941)
  AUTHORS   Wang,P., Sachar,M., Lu,J., Shehu,A.I., Zhu,J., Chen,J., Liu,K.,
            Anderson,K.E., Xie,W., Gonzalez,F.J., Klaassen,C.D. and Ma,X.
  TITLE     The essential role of the transporter ABCG2 in the pathophysiology
            of erythropoietic protoporphyria
  JOURNAL   Sci Adv 5 (9), eaaw6127 (2019)
   PUBMED   31555729
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2941)
  AUTHORS   Taketani,S., Mohri,T., Hioki,K., Tokunaga,R. and Kohno,H.
  TITLE     Structure and transcriptional regulation of the mouse
            ferrochelatase gene
  JOURNAL   Gene 227 (2), 117-124 (1999)
   PUBMED   10023040
REFERENCE   6  (bases 1 to 2941)
  AUTHORS   Shivdasani,R.A. and Orkin,S.H.
  TITLE     Erythropoiesis and globin gene expression in mice lacking the
            transcription factor NF-E2
  JOURNAL   Proc Natl Acad Sci U S A 92 (19), 8690-8694 (1995)
   PUBMED   7567998
REFERENCE   7  (bases 1 to 2941)
  AUTHORS   Tutois,S., Montagutelli,X., Da Silva,V., Jouault,H.,
            Rouyer-Fessard,P., Leroy-Viard,K., Guenet,J.L., Nordmann,Y.,
            Beuzard,Y. and Deybach,J.C.
  TITLE     Erythropoietic protoporphyria in the house mouse. A recessive
            inherited ferrochelatase deficiency with anemia, photosensitivity,
            and liver disease
  JOURNAL   J Clin Invest 88 (5), 1730-1736 (1991)
   PUBMED   1939658
REFERENCE   8  (bases 1 to 2941)
  AUTHORS   Brenner,D.A. and Frasier,F.
  TITLE     Cloning of murine ferrochelatase
  JOURNAL   Proc Natl Acad Sci U S A 88 (3), 849-853 (1991)
   PUBMED   1704134
REFERENCE   9  (bases 1 to 2941)
  AUTHORS   Taketani,S., Nakahashi,Y., Osumi,T. and Tokunaga,R.
  TITLE     Molecular cloning, sequencing, and expression of mouse
            ferrochelatase
  JOURNAL   J Biol Chem 265 (32), 19377-19380 (1990)
   PUBMED   2246229
REFERENCE   10 (bases 1 to 2941)
  AUTHORS   Bannerman,R.M., Edwards,J.A. and Pinkerton,P.H.
  TITLE     Hereditary disorders of the red cell in animals
  JOURNAL   Prog Hematol 8, 131-179 (1973)
   PUBMED   4596202
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102268.11, AC102106.14 and AEKQ02072540.1.
            
            On Jul 30, 2020 this sequence version replaced NM_007998.7.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159652.1, AK004718.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               AC102268.11        26353-26550         c
            199-322             AC102268.11        20786-20909         c
            323-442             AC102268.11        16103-16222         c
            443-591             AC102106.14        1855-2003
            592-726             AC102106.14        6244-6378
            727-833             AC102106.14        9226-9332
            834-932             AC102106.14        12876-12974
            933-1040            AC102106.14        14846-14953
            1041-1205           AC102106.14        15375-15539
            1206-1265           AC102106.14        18346-18405
            1266-2250           AC102106.14        18843-19827
            2251-2451           AEKQ02072540.1     16993-17193
            2452-2941           AC102106.14        20029-20518
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 37.43 cM"
     gene            1..2941
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /note="ferrochelatase"
                     /db_xref="GeneID:14151"
                     /db_xref="MGI:MGI:95513"
     exon            1..198
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..1400
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /EC_number="4.98.1.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ferrochelatase, mitochondrial; heme synthetase; protoheme
                     ferro-lyase; heme synthase"
                     /codon_start=1
                     /product="ferrochelatase, mitochondrial isoform 1"
                     /protein_id="NP_032024.2"
                     /db_xref="CCDS:CCDS29303.1"
                     /db_xref="GeneID:14151"
                     /db_xref="MGI:MGI:95513"
                     /translation="MLSASANMAAALRAAGALLREPLVHGSSRACQPWRCQSGAAVAA
                     TTEKVHHAKTTKPQAQPERRKPKTGILMLNMGGPETLGEVQDFLQRLFLDRDLMTLPI
                     QNKLAPFIAKRRTPKIQEQYRRIGGGSPIKMWTSKQGEGMVKLLDELSPATAPHKYYI
                     GFRYVHPLTEEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNEVGQKPTM
                     KWSTIDRWPTHPLLIQCFADHILKELNHFPEEKRSEVVILFSAHSLPMSVVNRGDPYP
                     QEVGATVHKVMEKLGYPNPYRLVWQSKVGPVPWLGPQTDEAIKGLCERGRKNILLVPI
                     AFTSDHIETLYELDIEYSQVLAQKCGAENIRRAESLNGNPLFSKALADLVHSHIQSNK
                     LCSTQLSLNCPLCVNPVCRKTKSFFTSQQL"
     exon            199..322
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            323..442
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            443..591
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            592..726
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            727..833
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            834..932
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1040
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1205
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1265
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..2941
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2199..2204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /note="hexamer: AATAAA"
     polyA_site      2218
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /note="major polyA site"
     regulatory      2903..2908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
                     /note="hexamer: AATAAT"
     polyA_site      2941
                     /gene="Fech"
                     /gene_synonym="fch; Fcl"
ORIGIN      
        1 ggccccgccc cggcgccgac ggcgccgggc gcagtgatca ggcgctgtgg cgcgggtgcg
       61 gagcgctgcc aggtcggcgt tcccgggcct tgccggagcg ccgccgcaga ggatccctgg
      121 ccgtcccgga aatgctttcg gccagcgcca acatggctgc ggccctgcgg gctgcgggcg
      181 ctctgctccg cgagccgctg gtgcacggca gctcaagggc ctgtcagcca tggaggtgcc
      241 agtcgggtgc tgcggtggca gccaccacgg agaaggtaca tcatgccaag accaccaaac
      301 cccaagctca accagaaagg aggaagccaa aaacgggcat attgatgtta aacatgggag
      361 gccccgaaac ccttggagaa gttcaagact ttcttcagag gctcttcctg gaccgagacc
      421 tcatgacact tcccattcaa aataagctgg caccattcat cgccaaacgc cgaaccccca
      481 aaattcaaga gcagtatcgc agaatcggag gtggatcccc catcaagatg tggacttcca
      541 agcaaggaga aggcatggtg aagctgctgg atgagttatc ccctgccaca gcacctcaca
      601 aatactatat tggattccgg tacgtccatc ccctgacaga agaggcaatt gaagagatgg
      661 agagagatgg actagagagg gccattgctt tcacacagta tccacagtat agctgctcca
      721 ccacaggcag cagcttaaat gccatttaca gatactataa cgaggtgggc cagaagccca
      781 ccatgaagtg gagcacaatc gacaggtggc ccacacaccc cctcctcatc cagtgctttg
      841 cagaccacat tctgaaagag ctgaaccatt ttccagagga gaagagaagc gaggtggtca
      901 ttctgttttc tgcccactcc ctgccgatgt ctgttgtcaa cagaggggac ccctatcccc
      961 aagaggtagg agccactgtc cacaaagtca tggaaaagct gggttacccc aacccctacc
     1021 gactggtttg gcagtccaag gttggtccag taccctggtt gggccctcag acagatgagg
     1081 ctatcaaagg gctttgtgag cgggggagga agaacatcct cttggttcca atagcattta
     1141 ccagtgacca cattgagacg ctctacgaac tggatattga atactctcaa gtgttggctc
     1201 agaagtgtgg agctgagaac atcagaagag cggagtctct taatggaaat ccattgttct
     1261 ccaaggccct ggctgatctg gtgcactccc acatccagtc caacaagctg tgctccacgc
     1321 agttgagtct gaactgtccg ctctgtgtaa atcctgtctg caggaagact aaatccttct
     1381 tcaccagcca acagctgtga ccctgcctga ggacccgtgg gatttgcaga tgtgcagcct
     1441 ccagacacct gcggtgggga cgatggggac agaagccacg tttgtctggg cacaaactgt
     1501 gatttctctc ttgcctgtgt tttatcttat tttatcttat ttctcgcttg ccctatgtag
     1561 gccaaactgg cttccagtgt gcctcccgct cccgtttcct gggcgctagt tagcatcaca
     1621 gacatacaac attgtgttcc ttggaggata gactcgaaat tcttactgag atttctattt
     1681 tcatatacaa gtatagtgac tgatcctggg gtcaatttat caatatggaa tatctgtcta
     1741 ctgggacatc ccaggaatga gtttcacagt tttattttat gagtcgcaca caaattctac
     1801 acttattgcc caaaacaact tcctacaaca ttgtggtgtg tctcctcatg tgctaccagt
     1861 ggtgactgag atgtttacag tcttatggat atagctatca cttaagtgag tattgtgagt
     1921 ttatgaaatg taatttgtaa aagaattagg ctgagcgcac agcttgggat catgtgctca
     1981 cctgcatcaa acacaaaaca cgatggtacc tactcttttc ccgagtccat ggctacggga
     2041 gtgtgagtga gttggagcgg tcacctggca ctgcttctgt tgacagtgta gccactgtcc
     2101 actgccactg tggtgactgc aagagtctca tctccctccc tggaaactga aacaagtgca
     2161 tgtttcaaga gtgctaagat tccccgggcg catagcctaa taaacgtgtg caagttcagt
     2221 ctttgctgtg tacgccttca tttgagtcca cagtgcctca gggaagggga ctctgccttc
     2281 ttcctgtctc cagggacagt gccaccagga aatgctactg atggatatgg gcttagctcc
     2341 ccaggggcca aggaaccttc cagtgccatc ctaatcttga ctgagtgctt attttataaa
     2401 gttgactgtg tctgatagcc ttgaattttc tttgtgaata agttcttaat ataaaaatta
     2461 catggttata gactaggcca tttgtaaaac acaaagaaat aactaatgta ataattatca
     2521 ttttattcta atcttttcct gcatatatgt gtgtatacac acacacatat atacatacat
     2581 atatatatgt atatatatat atatacacac acacatacat atatatacat acacatatat
     2641 atgcacatac acatacatgc atgcatatat agacatatat acacatacat acatatacac
     2701 atacatatat gcacatacat atatgtatag tctccaacat atttcatcat atcaacctcc
     2761 tttcccctca taactaattc ttgcctgaat ttgtaatgaa atgttattct aaaaatgtaa
     2821 aaactatgtc aaagcagaat ctgctcttct atatttggat ttaaatggat ttgaaagtct
     2881 ttgggatcct tgaaggattt gtaataatca ctttctctaa aactttgctc ttcaaacaga
     2941 a
//
