LOCUS       NM_134099               3479 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus F-box protein 4 (Fbxo4), mRNA.
ACCESSION   NM_134099
VERSION     NM_134099.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Peng,J., Li,Y., Wang,X., Deng,S., Holland,J., Yates,E., Chen,J.,
            Gu,H., Essandoh,K., Mu,X., Wang,B., McNamara,R.K., Peng,T.,
            Jegga,A.G., Liu,T., Nakamura,T., Huang,K., Perez-Tilve,D. and
            Fan,G.C.
  TITLE     An Hsp20-FBXO4 Axis Regulates Adipocyte Function through Modulating
            PPARgamma Ubiquitination
  JOURNAL   Cell Rep 23 (12), 3607-3620 (2018)
   PUBMED   29925002
  REMARK    GeneRIF: These findings suggest a role of Hsp20 in mediating
            adipocyte function by linking beta-adrenergic signaling to
            PPARgamma activity.
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Qie,S., Majumder,M., Mackiewicz,K., Howley,B.V., Peterson,Y.K.,
            Howe,P.H., Palanisamy,V. and Diehl,J.A.
  TITLE     Fbxo4-mediated degradation of Fxr1 suppresses tumorigenesis in head
            and neck squamous cell carcinoma
  JOURNAL   Nat Commun 8 (1), 1534 (2017)
   PUBMED   29142209
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3479)
  AUTHORS   Pytel,D., Gao,Y., Mackiewicz,K., Katlinskaya,Y.V., Staschke,K.A.,
            Paredes,M.C., Yoshida,A., Qie,S., Zhang,G., Chajewski,O.S., Wu,L.,
            Majsterek,I., Herlyn,M., Fuchs,S.Y. and Diehl,J.A.
  TITLE     PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines
            Tumor-Suppressive Versus Tumor Promoting Properties of PERK in
            Melanoma
  JOURNAL   PLoS Genet 12 (12), e1006518 (2016)
   PUBMED   27977682
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3479)
  AUTHORS   Lian,Z., Lee,E.K., Bass,A.J., Wong,K.K., Klein-Szanto,A.J.,
            Rustgi,A.K. and Diehl,J.A.
  TITLE     FBXO4 loss facilitates carcinogen induced papilloma development in
            mice
  JOURNAL   Cancer Biol Ther 16 (5), 750-755 (2015)
   PUBMED   25801820
  REMARK    GeneRIF: Collectively, our data support a role for FBXO4 as a
            suppressor of esophageal tumorigenesis
REFERENCE   5  (bases 1 to 3479)
  AUTHORS   Lee,E.K., Lian,Z., D'Andrea,K., Letrero,R., Sheng,W., Liu,S.,
            Diehl,J.N., Pytel,D., Barbash,O., Schuchter,L., Amaravaradi,R.,
            Xu,X., Herlyn,M., Nathanson,K.L. and Diehl,J.A.
  TITLE     The FBXO4 tumor suppressor functions as a barrier to
            BRAFV600E-dependent metastatic melanoma
  JOURNAL   Mol Cell Biol 33 (22), 4422-4433 (2013)
   PUBMED   24019069
  REMARK    GeneRIF: This study demonstrates that Fbxo4 deficiency induces
            Braf-driven melanoma and that this phenotype depends on cyclin D1
            accumulation in mice, underscoring the importance of this ubiquitin
            ligase in tumor suppression.
REFERENCE   6  (bases 1 to 3479)
  AUTHORS   Vaites,L.P., Lee,E.K., Lian,Z., Barbash,O., Roy,D., Wasik,M.,
            Klein-Szanto,A.J., Rustgi,A.K. and Diehl,J.A.
  TITLE     The Fbx4 tumor suppressor regulates cyclin D1 accumulation and
            prevents neoplastic transformation
  JOURNAL   Mol Cell Biol 31 (22), 4513-4523 (2011)
   PUBMED   21911473
  REMARK    GeneRIF: Fbx4 expression is essential for cell homeostasis in
            somatic cells. Fbx4 ablation predisposes mice to multiple tumor
            phenotypes, with concomitant cyclin D1 deregulation and nuclear
            accumulation
REFERENCE   7  (bases 1 to 3479)
  AUTHORS   Barbash,O., Lee,E.K. and Diehl,J.A.
  TITLE     Phosphorylation-dependent regulation of SCF(Fbx4) dimerization and
            activity involves a novel component, 14-3-3varepsilon
  JOURNAL   Oncogene 30 (17), 1995-2002 (2011)
   PUBMED   21242966
REFERENCE   8  (bases 1 to 3479)
  AUTHORS   Barbash,O., Egan,E., Pontano,L.L., Kosak,J. and Diehl,J.A.
  TITLE     Lysine 269 is essential for cyclin D1 ubiquitylation by the
            SCF(Fbx4/alphaB-crystallin) ligase and subsequent
            proteasome-dependent degradation
  JOURNAL   Oncogene 28 (49), 4317-4325 (2009)
   PUBMED   19767775
  REMARK    GeneRIF: Lysine 269 is essential for cyclin D1 ubiquitylation by
            the SCF(Fbx4/alphaB-crystallin) ligase.
REFERENCE   9  (bases 1 to 3479)
  AUTHORS   Lin,D.I., Barbash,O., Kumar,K.G., Weber,J.D., Harper,J.W.,
            Klein-Szanto,A.J., Rustgi,A., Fuchs,S.Y. and Diehl,J.A.
  TITLE     Phosphorylation-dependent ubiquitination of cyclin D1 by the
            SCF(FBX4-alphaB crystallin) complex
  JOURNAL   Mol Cell 24 (3), 355-366 (2006)
   PUBMED   17081987
  REMARK    GeneRIF: SCF(FBX4-alphaB crystallin) is an E3 ubiquitin ligase that
            promotes ubiquitin-dependent degradation of Thr286-phosphorylated
            cyclin D1.
REFERENCE   10 (bases 1 to 3479)
  AUTHORS   Jin,J., Cardozo,T., Lovering,R.C., Elledge,S.J., Pagano,M. and
            Harper,J.W.
  TITLE     Systematic analysis and nomenclature of mammalian F-box proteins
  JOURNAL   Genes Dev 18 (21), 2573-2580 (2004)
   PUBMED   15520277
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC137902.10, BC036385.1 and AI480454.1.
            
            On Aug 27, 2009 this sequence version replaced NM_134099.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC040086.1, SRR1660825.93763.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AC137902.10        166934-166996       c
            64-524              BC036385.1         30-490
            525-525             AC137902.10        165228-165228       c
            526-1543            BC036385.1         491-1508
            1544-3420           AC137902.10        151013-152889       c
            3421-3479           AI480454.1         1-59                c
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 1.84 cM"
     gene            1..3479
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /note="F-box protein 4"
                     /db_xref="GeneID:106052"
                     /db_xref="MGI:MGI:2146220"
     exon            1..332
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1307
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /codon_start=1
                     /product="F-box only protein 4"
                     /protein_id="NP_598860.2"
                     /db_xref="CCDS:CCDS27359.1"
                     /db_xref="GeneID:106052"
                     /db_xref="MGI:MGI:2146220"
                     /translation="MAGSEPRGAGSPPPASDWGRLEAAILSGWRTFWYSVAKERATPT
                     ASRKEAAEETSALTRLPVDVQLYILSFLSPHDLCQLGSTDHYWNKTIRDPILWRYFLL
                     RDLPSWSSVDWKSLPDLEILKKPISEVTDSTCLDYMEVYKMCCPYTRRALKASRPMYG
                     VVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEDLCPTAGLPHRQIDGIGSG
                     VNFQLNNQQKFNILILYSTTRKERDRAREEHTSTVNKMFSLQSEGDEQQGSRYSVIPQ
                     IQKVCEVVDGFIYVANAEAHRRHEWQDEFSRIMAMTDPAFGSSGRPMLVLSCISQADV
                     KRMPCFYLAHELHLSLLNHPWMVQDTEAETLTGFLNGIEWILEEVESKRAK"
     exon            333..568
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     exon            569..789
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     exon            790..865
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1041
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1217
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..3446
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1529..1534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /note="hexamer: ATTAAA"
     polyA_site      1549
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /note="major polyA site"
     regulatory      2003..2008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /note="hexamer: TATAAA"
     polyA_site      2025
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
     regulatory      3405..3410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /note="hexamer: AATAAA"
     regulatory      3409..3414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
                     /note="hexamer: AATATA"
     polyA_site      3424
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
     polyA_site      3446
                     /gene="Fbxo4"
                     /gene_synonym="1700096C12Rik; Fbx4"
ORIGIN      
        1 gtttgggcta acggggtgag tcattccggg ccacctcggc ctggagccct gcccaaccga
       61 ccagcccggg tcgtaccagc ggggcggagg cggaggccga gcggctgggg agcccgtgcg
      121 ccccgcggtg tcgcgcggag cctgcgacca tggctggaag cgagccccgc ggagccggct
      181 ccccgccgcc cgccagcgac tggggccgcc tggaggcagc catcctgagc ggctggagga
      241 ccttctggta ttcggtggcc aaggagcggg cgacgccgac ggcctcccgg aaggaggcgg
      301 cggaggagac gagcgcgctg acgcggctgc cggttgatgt gcagttgtat atcttgtcgt
      361 ttctttcacc ccacgatctg tgccagctgg gaagtacaga tcattactgg aacaaaacta
      421 taagagaccc aattctctgg agatactttc tgttgcggga tctcccttct tggtcttcgg
      481 ttgattggaa gtcacttcca gatctagaga tcttaaaaaa gccaatatct gaggtcaccg
      541 acagcacttg tcttgattac atggaggttt ataaaatgtg ctgtccatat acgcgaagag
      601 ccttgaaagc cagccgtcct atgtatggag tggttacctc tttcttacac tcactgatca
      661 ttcagaatga accccggttt gctatgtttg gaccaggttt ggaagaactg aacacatcct
      721 tggtgttgag tttgatgtct tctgaggacc tttgcccaac tgctggttta cctcacagac
      781 agattgatgg tattggatct ggagtcaact tccagttgaa taaccagcaa aaattcaaca
      841 tcctgatatt atactcgact accagaaaag aaagagacag agcaagggag gagcacacca
      901 gcaccgttaa caagatgttc agcctacaga gtgaggggga cgagcagcag ggcagccgct
      961 acagtgtgat cccgcagatt cagaaagtgt gtgaagtcgt agacgggttc atctacgtgg
     1021 caaacgctga agctcaccga cgtcatgaat ggcaagatga attttctcgg attatggcca
     1081 tgacagaccc agcttttgga tcttcaggaa gacccatgct ggttttatct tgtatttctc
     1141 aagcagatgt aaagagaatg ccttgttttt atttagctca tgagctgcac ctcagtcttc
     1201 taaaccaccc atggatggtc caggatacag aggctgaaac tctgactggt tttttgaatg
     1261 gcattgagtg gattcttgaa gaagtagaat ctaagcgtgc aaaatgaagt tcctttggga
     1321 ccatgggaag ccgcaggaca tggtgtatgt gtttcctgtc cctcctgtca ctgcccctct
     1381 ggaggaggcc tttctactgg acagctacca cagctgggtt tctgacactt taccatgaat
     1441 gagttgcaag tttcagtcct actacttttc cacaaaatga actctcacat ttttgataat
     1501 tttatatttt ttgtcttttt ctttaaatat taaattccgc aaaacaaaat tttgtggttc
     1561 tttaatttct catggtctct cttttttgct atcaaggttg cccgatagga atagtaagcc
     1621 tttttaacag tcataggatc tcttcaaggt ctcactaact gcttatcgga aaaacgtttc
     1681 aaatcattct caacagagac actatgaaat ttcactagaa ctgcagtaat gtaatgacca
     1741 cagcctctgt tctctttcga aagaatagaa agaatattat gtttatgttg tatctgatat
     1801 tctttcagtg aaaattaggg tttttgttgt ggaagttgtc atgtttgaat cattctgagc
     1861 aaggaaatac taaattctgt ttcatactta tacatgctaa agatttacat gtgtaaagct
     1921 acctcagcaa ttgcttgcac tgcagaggaa atcacatagc acctgactgg aactgggggt
     1981 gttaaaatta ttgtgaaagg aatataaata ctgttgtatg atttatatgt ttgtatttaa
     2041 gtgtacttaa atatgtttgt tttgtggaga aagtgaccac agactttcta aatattctaa
     2101 atatgcgttg agaattatga aggacagaag gagagtgttc aggcaatata attgatctgg
     2161 taccagggtt caagggtgaa tggtgggtgc aaagaagttg gaagaacatg gtaagaaggc
     2221 tgcccttcat atagaagtaa ggtggtaaca gaagcagttg tgtgcaggtt agggtccaat
     2281 ttagtaagat tatacgttaa gatcttggag ccaacagttt tgagtgacga taactttcac
     2341 actaaccccg ggagcagagg tgggaggttt ggaggtcagt gctgtagctg aggccaagga
     2401 gctcacaatc agatggcaga aacactgtaa gacagaggtg atgatgaggc aggtggcagg
     2461 aggcattctc aggaagccat tcagtcatct cagagctctg tggtgctctg tactgaaaat
     2521 tcctgtgatg acgaaagcct tcaagggcta cactgtctag gatgcacagc ctctagccat
     2581 gaggacttga aatgtgcctc aggcaactga ggaactgact tgtaaatatt atgcaaattt
     2641 aaatcattgt actaccttta atcacacaaa actagtagct attacaatct gctagcattg
     2701 caagaagaca atataatttc ttatcacagt ttatattgag cagatttctt aatatttcac
     2761 atacagtgaa agagaccttc ccttatacaa ataagacttc atatcctata agaactttga
     2821 tgaataattt ttttctttaa atagcccatc tttaaaataa attttaaaaa gtaattttta
     2881 taacaatttt acctttttta ttagacattt tctttatatt tcaaatatta ttccctttct
     2941 tggtttcccc tctgctgaag agttattgtg cattttaaaa caggctgaac atgtttcaga
     3001 gactcattta acccattgtc ttagtgggaa ggttttacat gaaaaacaga gtatctctaa
     3061 tgaatagata aaagaacata gtatccaaaa taagatattt cttcttgggt atgtattggt
     3121 tgtacttttc aagcaatttt ttccatatca tataaattgc tcattttatt ggcataaggt
     3181 tgtaaatagc attcattaac ttttctaaaa attgtacaac acagtaatca gtatgctccc
     3241 aattctatgg ttagtgtagc agtattagat atctgtaact gaagtggaaa cttctgggat
     3301 cttggaaagt cagttatgtc aaattatgtt ttgctgggga atacaggtga aaggaggttt
     3361 tgtcaatgca gacatgtgaa agcatgtgtg atgttaagaa ggaaaataaa tatagtccaa
     3421 cagacgatgg gcaatgctgg atggtaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
