LOCUS       NM_024274               1896 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus phenylalanine-tRNA synthetase 2, mitochondrial
            (Fars2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_024274 XM_987226 XM_987259 XM_987292 XM_987317 XM_987355
            XM_987394 XM_987428
VERSION     NM_024274.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Li,B., Liu,F., Chen,X., Chen,T., Zhang,J., Liu,Y., Yao,Y., Hu,W.,
            Zhang,M., Wang,B., Liu,L., Chen,K. and Wu,Y.
  TITLE     FARS2 Deficiency Causes Cardiomyopathy by Disrupting Mitochondrial
            Homeostasis and the Mitochondrial Quality Control System
  JOURNAL   Circulation 149 (16), 1268-1284 (2024)
   PUBMED   38362779
  REMARK    GeneRIF: FARS2 Deficiency Causes Cardiomyopathy by Disrupting
            Mitochondrial Homeostasis and the Mitochondrial Quality Control
            System.
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1896)
  AUTHORS   Wang,X., Zhang,Y., Yu,J., Ma,Y., Xu,Y., Shi,J., Qi,Z. and Liu,X.
  TITLE     Identification and analysis of key circRNAs in the mouse embryonic
            ovary provides insight into primordial follicle development
  JOURNAL   BMC Genomics 25 (1), 139 (2024)
   PUBMED   38310234
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1896)
  AUTHORS   Griffin,E.N., Jucius,T., Sim,S.E., Harris,B.S., Heinz,S. and
            Ackerman,S.L.
  TITLE     RREB1 regulates neuronal proteostasis and the microtubule network
  JOURNAL   Sci Adv 10 (2), eadh3929 (2024)
   PUBMED   38198538
REFERENCE   5  (bases 1 to 1896)
  AUTHORS   Chen,X., Liu,F., Li,B., Wang,Y., Yuan,L., Yin,A., Chen,Q., Hu,W.,
            Yao,Y., Zhang,M., Wu,Y. and Chen,K.
  TITLE     Neuropathy-associated Fars2 deficiency affects neuronal development
            and potentiates neuronal apoptosis by impairing mitochondrial
            function
  JOURNAL   Cell Biosci 12 (1), 103 (2022)
   PUBMED   35794642
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY290041.1, BY101501.1, AK054370.1, AK020164.1, AK076259.1 and
            AC121942.2.
            
            On Oct 25, 2013 this sequence version replaced NM_024274.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK170986.1, BC002147.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY290041.1         1-11
            12-237              BY101501.1         1-226
            238-996             AK054370.1         3-761
            997-1812            AK020164.1         150-965
            1813-1893           AK076259.1         1598-1678
            1894-1896           AC121942.2         76840-76842
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 14.44 cM"
     gene            1..1896
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /note="phenylalanine-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:69955"
                     /db_xref="MGI:MGI:1917205"
     exon            1..363
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     exon            364..996
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     CDS             385..1740
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /EC_number="6.1.1.20"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phenylalanine-tRNA synthetase 1 (mitochondrial);
                     phenylalanine--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="phenylalanine--tRNA ligase, mitochondrial
                     isoform 1"
                     /protein_id="NP_077236.1"
                     /db_xref="CCDS:CCDS26453.1"
                     /db_xref="GeneID:69955"
                     /db_xref="MGI:MGI:1917205"
                     /translation="MVCLALVRAAYEHIYLVRKVSHACRCHQHRAWSSKPAASQSAVQ
                     GAPGSVLEILGKSYPQDDHTNLTQKVLSKVGRNLHNQKFHPLWLIKERVKEHFYQQYM
                     VRSRTPLFSVYDQLPPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRAHMLRAHTSAHQ
                     WDLLHAGLNAFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLF
                     EEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFE
                     MEINFRGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWS
                     EDERFLKQFLLSDINQSVKFQPLSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDL
                     VEKVDLIDKFEHPKTHRTSHCYRITYRHMERTLSQREVGNVHQAVQEAAVQLLGVEGR
                     F"
     exon            997..1156
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1288
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1449
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1601
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1896
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1870..1875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /note="hexamer: AATAAA"
     polyA_site      1894
                     /gene="Fars2"
                     /gene_synonym="Fars1; pheRS"
                     /note="major polyA site"
ORIGIN      
        1 agccctgccc ctgcccggct cggctgtctt tggcgctgcc tgacctgctg tgcacgaaaa
       61 cgagggtgac tttgctttgg atccgtctgg ttggcagcct gagtttatca gtgtcatcgt
      121 gactggggga cgcagcgcca gtggtgcgga ggtaactagc tggcgacttc ctggcgcacc
      181 ggcttgtctt tgggcgagcg gctcaaggga cctagggccc tggcctcttc ccgtcaagat
      241 ggcggcgccc agcgcgccca gcgtctcggt agccagaggt taagctcagt gacggcggag
      301 tgagtgtgtg gccgctttgc ggacttcgaa acgcccgcgt ttggtttccc cggcgtacgc
      361 tggagtttgt gagaagcttc tataatggtc tgcttggctc tcgtcagagc tgcctatgag
      421 cacatctacc tggtgaggaa ggtcagtcat gcctgcagat gccatcaaca tcgggcttgg
      481 agctcaaagc cagctgcatc acagtctgct gtccagggtg ctccaggcag tgtgctggag
      541 atacttggta aatcctaccc tcaggatgac cacactaacc tcacccagaa ggtcctttcc
      601 aaggtgggca ggaacctgca caaccagaag ttccaccctc tgtggctgat taaggagcgg
      661 gtgaaggagc acttctacca gcagtatatg gtgcgatcca ggacacctct attctccgtc
      721 tatgaccagc ttcctccagt ggtcaccacc tggcagaact ttgatagcct gctaatccca
      781 gctgaccacc ccagcaggaa aaagggggac aactattact tgaatcgggc acacatgctg
      841 agagcacaca catcagcgca tcagtgggac ttgctgcatg cgggacttaa tgccttcctt
      901 gtggtaggtg acgtgtatcg tcgtgaccag attgattgcc agcactaccc agttttccac
      961 cagctggagg gtgtccggct cttctccaag catgagttat ttgctggcgt aaaggatgga
     1021 gaaagcttgc agctctttga agaaggttct cgctctgctc ataagcagga gacacacacc
     1081 atggaggctg tgaagcttgt ggagttcgac cttaaacaag tactgactag gcttgtgaca
     1141 catctttttg gagatgggct ggaggttcgg tgggtggatt gctacttccc cttcacccac
     1201 ccttccttcg agatggagat caacttccgt ggagaatggc ttgaagtcct tggctgcggt
     1261 gtgatggagc agcagcttgt caattcagct ggtgctcagg atcgaatcgg ctgggcgttt
     1321 ggcctgggat tagaaagatt ggcgatggtc ctctacgaca ttcctgacat ccgccttttc
     1381 tggagtgagg atgagcgctt cctgaagcag ttcctcctgt cggacatcaa ccagagtgtc
     1441 aagtttcagc ctcttagcaa gtaccccgcc gtgttcaatg acatttcatt ctggctgccc
     1501 tctgagaact acacggagaa tgatttctat gacatcgtcc gaacagtcgg aggagacctg
     1561 gtagaaaagg ttgatcttat agacaagttt gaacatccaa agacacacag gaccagccac
     1621 tgctaccgca ttacctaccg ccacatggaa cggacactgt cccagagaga agtcggcaat
     1681 gtccaccagg ctgtgcagga ggccgccgtg cagctgctgg gtgtggaggg ccgtttctga
     1741 tgcagtggca gtccacaggc cacagccctc atagagctcc agggagcacc aggaaggggg
     1801 aaacacattg tttgtgaact ggagtgtctg ccctcacttg acagatgggt ctactttagg
     1861 acgtcacgaa ataaaggatc acttttcaga agcatt
//
