LOCUS       NM_007986               2704 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus fibroblast activation protein (Fap), mRNA.
ACCESSION   NM_007986
VERSION     NM_007986.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Amrute,J.M., Luo,X., Penna,V., Yang,S., Yamawaki,T., Hayat,S.,
            Bredemeyer,A., Jung,I.H., Kadyrov,F.F., Heo,G.S., Venkatesan,R.,
            Shi,S.Y., Parvathaneni,A., Koenig,A.L., Kuppe,C., Baker,C.,
            Luehmann,H., Jones,C., Kopecky,B., Zeng,X., Bleckwehl,T., Ma,P.,
            Lee,P., Terada,Y., Fu,A., Furtado,M., Kreisel,D., Kovacs,A.,
            Stitziel,N.O., Jackson,S., Li,C.M., Liu,Y., Rosenthal,N.A.,
            Kramann,R., Ason,B. and Lavine,K.J.
  TITLE     Targeting immune-fibroblast cell communication in heart failure
  JOURNAL   Nature 635 (8038), 423-433 (2024)
   PUBMED   39443792
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   He,J., Fang,B., Shan,S. and Li,Q.
  TITLE     Mechanical stiffness promotes skin fibrosis through FAPalpha-AKT
            signaling pathway
  JOURNAL   J Dermatol Sci 113 (2), 51-61 (2024)
   PUBMED   38155020
  REMARK    GeneRIF: Mechanical stiffness promotes skin fibrosis through
            FAPalpha-AKT signaling pathway.
REFERENCE   3  (bases 1 to 2704)
  AUTHORS   Wu,Y., Wu,C., Shi,T., Cai,Q., Wang,T., Xiong,Y., Zhang,Y.,
            Jiang,W., Lu,M., Chen,Z., Chen,J., Wang,J. and He,R.
  TITLE     FAP expression in adipose tissue macrophages promotes obesity and
            metabolic inflammation
  JOURNAL   Proc Natl Acad Sci U S A 120 (51), e2303075120 (2023)
   PUBMED   38100414
  REMARK    GeneRIF: FAP expression in adipose tissue macrophages promotes
            obesity and metabolic inflammation.
REFERENCE   4  (bases 1 to 2704)
  AUTHORS   Chen,M., Xiao,L., Jia,H., Wang,S., Jiang,X., Lei,X., Zhai,Q. and
            Lang,J.
  TITLE     Stereotactic ablative radiotherapy and FAPalpha-based cancer
            vaccine suppresses metastatic tumor growth in 4T1 mouse breast
            cancer
  JOURNAL   Radiother Oncol 189, 109946 (2023)
   PUBMED   37806560
  REMARK    GeneRIF: Stereotactic ablative radiotherapy and FAPalpha-based
            cancer vaccine suppresses metastatic tumor growth in 4T1 mouse
            breast cancer.
REFERENCE   5  (bases 1 to 2704)
  AUTHORS   Ramirez-Montagut,T., Blachere,N.E., Sviderskaya,E.V., Bennett,D.C.,
            Rettig,W.J., Garin-Chesa,P. and Houghton,A.N.
  TITLE     FAPalpha, a surface peptidase expressed during wound healing, is a
            tumor suppressor
  JOURNAL   Oncogene 23 (32), 5435-5446 (2004)
   PUBMED   15133496
REFERENCE   6  (bases 1 to 2704)
  AUTHORS   Ibrahim,S.M., Koczan,D. and Thiesen,H.J.
  TITLE     Gene-expression profile of collagen-induced arthritis
  JOURNAL   J Autoimmun 18 (2), 159-167 (2002)
   PUBMED   11908948
REFERENCE   7  (bases 1 to 2704)
  AUTHORS   Niedermeyer,J., Garin-Chesa,P., Kriz,M., Hilberg,F., Mueller,E.,
            Bamberger,U., Rettig,W.J. and Schnapp,A.
  TITLE     Expression of the fibroblast activation protein during mouse embryo
            development
  JOURNAL   Int J Dev Biol 45 (2), 445-447 (2001)
   PUBMED   11330865
REFERENCE   8  (bases 1 to 2704)
  AUTHORS   Niedermeyer,J., Kriz,M., Hilberg,F., Garin-Chesa,P., Bamberger,U.,
            Lenter,M.C., Park,J., Viertel,B., Puschner,H., Mauz,M., Rettig,W.J.
            and Schnapp,A.
  TITLE     Targeted disruption of mouse fibroblast activation protein
  JOURNAL   Mol Cell Biol 20 (3), 1089-1094 (2000)
   PUBMED   10629066
REFERENCE   9  (bases 1 to 2704)
  AUTHORS   Niedermeyer,J., Enenkel,B., Park,J.E., Lenter,M., Rettig,W.J.,
            Damm,K. and Schnapp,A.
  TITLE     Mouse fibroblast-activation protein--conserved Fap gene
            organization and biochemical function as a serine protease
  JOURNAL   Eur J Biochem 254 (3), 650-654 (1998)
   PUBMED   9688278
REFERENCE   10 (bases 1 to 2704)
  AUTHORS   Niedermeyer,J., Scanlan,M.J., Garin-Chesa,P., Daiber,C.,
            Fiebig,H.H., Old,L.J., Rettig,W.J. and Schnapp,A.
  TITLE     Mouse fibroblast activation protein: molecular cloning, alternative
            splicing and expression in the reactive stroma of epithelial
            cancers
  JOURNAL   Int J Cancer 71 (3), 383-389 (1997)
   PUBMED   9139873
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL928576.9.
            
            On Apr 20, 2013 this sequence version replaced NM_007986.2.
            
            Summary: This gene belongs to the serine protease family. The
            encoded protein is an inducible cell-surface bound glycoprotein
            specifically expressed in tumor-associated fibroblasts and
            pericytes of epithelial tumors and has protease and gelatinase
            activity. The protein plays a role in remodeling of the
            extracellular matrix (ECM) and may affect tumorigenesis and tissue
            repair. Alternately spliced transcript variants of this gene are
            described in the literature (PMID 9139873), but the full-length
            sequence of these variants is not available. [provided by RefSeq,
            Apr 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y10007.1, BC019190.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-176               AL928576.9         181666-181841       c
            177-261             AL928576.9         181259-181343       c
            262-360             AL928576.9         163921-164019       c
            361-455             AL928576.9         162565-162659       c
            456-530             AL928576.9         161603-161677       c
            531-583             AL928576.9         160094-160146       c
            584-656             AL928576.9         156390-156462       c
            657-777             AL928576.9         155575-155695       c
            778-932             AL928576.9         154378-154532       c
            933-1036            AL928576.9         152107-152210       c
            1037-1172           AL928576.9         149951-150086       c
            1173-1217           AL928576.9         144810-144854       c
            1218-1322           AL928576.9         140951-141055       c
            1323-1393           AL928576.9         140782-140852       c
            1394-1447           AL928576.9         138528-138581       c
            1448-1572           AL928576.9         136475-136599       c
            1573-1620           AL928576.9         132013-132060       c
            1621-1719           AL928576.9         126814-126912       c
            1720-1789           AL928576.9         126287-126356       c
            1790-1984           AL928576.9         125340-125534       c
            1985-2039           AL928576.9         120206-120260       c
            2040-2139           AL928576.9         112326-112425       c
            2140-2204           AL928576.9         111185-111249       c
            2205-2277           AL928576.9         110620-110692       c
            2278-2351           AL928576.9         110262-110335       c
            2352-2704           AL928576.9         108756-109108       c
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 35.85 cM"
     gene            1..2704
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /note="fibroblast activation protein"
                     /db_xref="GeneID:14089"
                     /db_xref="MGI:MGI:109608"
     exon            1..176
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..2456
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /EC_number="3.4.14.5"
                     /EC_number="3.4.21.26"
                     /note="seprase; integral membrane serine protease;
                     fibroblast activation protein alpha; FAPalpha; dipeptidyl
                     peptidase FAP; surface-expressed protease; post-proline
                     cleaving enzyme; gelatine degradation protease FAP; serine
                     integral membrane protease"
                     /codon_start=1
                     /product="prolyl endopeptidase FAP"
                     /protein_id="NP_032012.1"
                     /db_xref="CCDS:CCDS16067.1"
                     /db_xref="GeneID:14089"
                     /db_xref="MGI:MGI:109608"
                     /translation="MKTWLKTVFGVTTLAALALVVICIVLRPSRVYKPEGNTKRALTL
                     KDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATD
                     YGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGS
                     KLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDG
                     KFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVG
                     PMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPK
                     NQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITS
                     GKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQY
                     YTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEI
                     KKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEG
                     IVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWS
                     YGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTV
                     MARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGR
                     SQNHLYTHMTHFLKQCFSLSD"
     exon            177..261
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            262..360
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            361..455
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            456..530
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            531..583
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            584..656
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            657..777
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            778..932
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1036
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1172
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1217
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1322
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1393
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1447
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1572
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1620
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1719
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1789
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1984
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2039
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2139
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2204
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2277
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2351
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2704
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2675..2680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /note="hexamer: AATAAA"
     polyA_site      2697
                     /gene="Fap"
                     /gene_synonym="SIMP"
                     /note="major polyA site"
ORIGIN      
        1 acggtagtca aaagtccgtg ggaaagggag gaggggagcg ggaagcaact catgtcctga
       61 cttcagcttc caagtggaag acagacttgc ttcttttcaa cagttttcac agatgcggtg
      121 acccacgctg tgcagtgaga atcagctaac ttgcaaaaac atctggaaaa atgaagacat
      181 ggctgaaaac tgtctttgga gttaccaccc tggctgcgct tgctttagtg gtgatatgca
      241 ttgtcttacg tccctcaaga gtttacaaac ctgaaggaaa cacaaagaga gctcttacct
      301 tgaaggatat tttaaatgga acattctcat ataaaacata ttttcccaac tggatttcag
      361 aacaagaata tcttcatcaa tctgaggatg ataacatagt attttataat attgaaacaa
      421 gagaatcata tatcattttg agtaatagca ccatgaaaag tgtgaatgct acagattatg
      481 gtttgtcacc tgatcggcaa tttgtgtatc tagaaagtga ttattcaaag ctctggcgat
      541 attcatacac agcgacatac tacatctacg accttcagaa tggggaattt gtaagaggat
      601 acgagctccc tcgtccaatt cagtatctat gctggtcgcc tgttgggagt aaattagcat
      661 atgtatatca aaacaatatt tatttgaaac aaagaccagg agatccacct tttcaaataa
      721 cttatactgg aagagaaaat agaatattta atggaatacc agactgggtt tatgaagagg
      781 aaatgcttgc cacaaaatat gctctttggt ggtctccaga tggaaaattt ttggcatatg
      841 tagaatttaa tgattcagat ataccaatta ttgcctattc ttattatggt gatggacagt
      901 atcctagaac tataaatatt ccatatccaa aggctggggc taagaatccg gttgttcgtg
      961 tttttattgt tgacaccacc taccctcacc acgtgggccc aatggaagtg ccagttccag
     1021 aaatgatagc ctcaagtgac tattatttca gctggctcac atgggtgtcc agtgaacgag
     1081 tatgcttgca gtggctaaaa agagtgcaga atgtctcagt cctgtctata tgtgatttca
     1141 gggaagactg gcatgcatgg gaatgtccaa agaaccagga gcatgtagaa gaaagcagaa
     1201 caggatgggc tggtggattc tttgtttcga caccagcttt tagccaggat gccacttctt
     1261 actacaaaat atttagcgac aaggatggtt acaaacatat tcactacatc aaagacactg
     1321 tggaaaatgc tattcaaatt acaagtggca agtgggaggc catatatata ttccgcgtaa
     1381 cacaggattc actgttttat tctagcaatg aatttgaagg ttaccctgga agaagaaaca
     1441 tctacagaat tagcattgga aactctcctc cgagcaagaa gtgtgttact tgccatctaa
     1501 ggaaagaaag gtgccaatat tacacagcaa gtttcagcta caaagccaag tactatgcac
     1561 tcgtctgcta tggccctggc ctccccattt ccaccctcca tgatggccgc acagaccaag
     1621 aaatacaagt attagaagaa aacaaagaac tggaaaattc tctgagaaat atccagctgc
     1681 ctaaagtgga gattaagaag ctcaaagacg ggggactgac tttctggtac aagatgattc
     1741 tgcctcctca gtttgacaga tcaaagaagt accctttgct aattcaagtg tatggtggtc
     1801 cttgcagcca gagtgttaag tctgtgtttg ctgttaattg gataacttat ctcgcaagta
     1861 aggaggggat agtcattgcc ctggtagatg gtcggggcac tgctttccaa ggtgacaaat
     1921 tcctgcatgc cgtgtatcga aaactgggtg tatatgaagt tgaggaccag ctcacagctg
     1981 tcagaaaatt catagaaatg ggtttcattg atgaagaaag aatagccata tggggctggt
     2041 cctacggagg ttatgtttca tccctggccc ttgcatctgg aactggtctt ttcaaatgtg
     2101 gcatagcagt ggctccagtc tccagctggg aatattacgc atctatctac tcagagagat
     2161 tcatgggcct cccaacaaag gacgacaatc tcgaacacta taaaaattca actgtgatgg
     2221 caagagcaga atatttcaga aatgtagact atcttctcat ccacggaaca gcagatgata
     2281 atgtgcactt tcagaactca gcacagattg ctaaagcttt ggttaatgca caagtggatt
     2341 tccaggcgat gtggtactct gaccagaacc atggtatatc atctgggcgc tcccagaatc
     2401 atttatatac ccacatgacg cacttcctca agcaatgctt ttctttatca gactgaacca
     2461 atgcaagtac tagcgtgtag gacagtctga aggcttcaca tggacgactt ggaccatgaa
     2521 tattgtaaaa gccgctaaaa atttaggagg gctcaggagg ttaggctcgg tattgtttac
     2581 atttccctat gctctgtgaa agaagagaaa aggggagcca cacatcttgc tttggacaca
     2641 atgttttatc acctgttcat tgccgggtag cggaaataaa gtcagatgct caggttatag
     2701 cgtg
//
