LOCUS       NM_178390               2028 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus family with sequence similarity 53, member A (Fam53a),
            transcript variant 2, mRNA.
ACCESSION   NM_178390
VERSION     NM_178390.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Kole,C., Berdugo,N., Da Silva,C., Ait-Ali,N., Millet-Puel,G.,
            Pagan,D., Blond,F., Poidevin,L., Ripp,R., Fontaine,V., Wincker,P.,
            Zack,D.J., Sahel,J.A., Poch,O. and Leveillard,T.
  TITLE     Identification of an Alternative Splicing Product of the Otx2 Gene
            Expressed in the Neural Retina and Retinal Pigmented Epithelial
            Cells
  JOURNAL   PLoS One 11 (3), e0150758 (2016)
   PUBMED   26985665
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2028)
  AUTHORS   Georgas,K., Rumballe,B., Valerius,M.T., Chiu,H.S.,
            Thiagarajan,R.D., Lesieur,E., Aronow,B.J., Brunskill,E.W.,
            Combes,A.N., Tang,D., Taylor,D., Grimmond,S.M., Potter,S.S.,
            McMahon,A.P. and Little,M.H.
  TITLE     Analysis of early nephron patterning reveals a role for distal RV
            proliferation in fusion to the ureteric tip via a cap
            mesenchyme-derived connecting segment
  JOURNAL   Dev Biol 332 (2), 273-286 (2009)
   PUBMED   19501082
REFERENCE   4  (bases 1 to 2028)
  AUTHORS   Combes,A.N., Lesieur,E., Harley,V.R., Sinclair,A.H., Little,M.H.,
            Wilhelm,D. and Koopman,P.
  TITLE     Three-dimensional visualization of testis cord morphogenesis, a
            novel tubulogenic mechanism in development
  JOURNAL   Dev Dyn 238 (5), 1033-1041 (2009)
   PUBMED   19334288
REFERENCE   5  (bases 1 to 2028)
  AUTHORS   Jun,L., Balboni,A.L., Laitman,J.T. and Bergemann,A.D.
  TITLE     Isolation of DNTNP, which encodes a potential nuclear protein that
            is expressed in the developing, dorsal neural tube
  JOURNAL   Dev Dyn 224 (1), 116-123 (2002)
   PUBMED   11984880
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB848576.1, AK163326.1 and BC028656.1.
            
            On Jul 23, 2009 this sequence version replaced NM_178390.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, and 3 all encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028656.1, AK163326.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                BB848576.1         2-31
            31-753              AK163326.1         2-724
            754-2000            AK163326.1         726-1972
            2001-2028           BC028656.1         1721-1748
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 17.83 cM"
     gene            1..2028
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /note="family with sequence similarity 53, member A"
                     /db_xref="GeneID:74504"
                     /db_xref="MGI:MGI:1919225"
     exon            1..299
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /inference="alignment:Splign:2.1.0"
     exon            300..424
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /inference="alignment:Splign:2.1.0"
     exon            425..656
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /inference="alignment:Splign:2.1.0"
     CDS             579..1787
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /note="isoform a is encoded by transcript variant 2;
                     dorsal neural-tube nuclear protein; protein FAM53A"
                     /codon_start=1
                     /product="protein FAM53A isoform a"
                     /protein_id="NP_848477.2"
                     /db_xref="CCDS:CCDS19203.1"
                     /db_xref="GeneID:74504"
                     /db_xref="MGI:MGI:1919225"
                     /translation="MVTLITEKLQNQSLDDLTRRACEAGPYSAEKLNKSGHLFPLEIS
                     VDKSPWKALRGGWPTGSQAASGPFSVGPHGVSHTEGLKWQLESPGPMDVGHFLDLHDS
                     TGPPAAPPTKRHCRSLSEPEELARCRSPWRPGSSKVWTPISKRRCNSGGSATLQCCSG
                     VGNPTLQGTLVPGLPRRPVSPAGPTSPLTPRPASASSGFVDGSEGSTSSGPPWLSTGP
                     CPFSSRRRLSLSQEHLVDTGACLPSASSTPTSTPELGRHHGLLRCRSQPCVLDGRRVR
                     RKRRREEDARWTRPSLDFLKMTRTLKNSKSLCSLDYEDDEDDTQEKTLVSSPCNSQGL
                     VGIITPSSSPRIPRPGPDSPSIWASGEPEANPGEGGSSGDPSDWDSAGEEGIFPLDHG
                     DLDLEQIENN"
     exon            657..711
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /inference="alignment:Splign:2.1.0"
     exon            712..1475
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..2003
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1982..1987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /note="hexamer: ATTAAA"
     polyA_site      2003
                     /gene="Fam53a"
                     /gene_synonym="2410018C17Rik; 5430419M09Rik; Dntnp"
                     /note="major polyA site"
ORIGIN      
        1 ggttttgttc cgcggtgact gaggctcacc gtccccatgc gcggagcgcg gcctgcgcgg
       61 ccggcgccct gccccagcta cggagctcgg tgctcactcg cctcgcggag agtgctctgc
      121 gccgtcgttc gcctgctcat cgctaagagc tccagccacc gcaacgccgt gtccttgcgc
      181 tgcgatcccg gagtctccgt acgtcggccg gcgccgcgca gccgcacgtc gagagccggg
      241 tgcatcggca cagcgcgcgg aacaccctct agggccagcc ggcgtctcag caccttaagg
      301 aagcaggaag tttgacgaga tatgatggtc ccttaaggga atcccaagag cagatcctcc
      361 ccaacgacct gtggaaggtg acttggaagt ctattccgaa gccactttcc tgcgtagact
      421 gaggagacaa agccctgaag agtctggaaa tgggtccgca tctttaagtc agagctataa
      481 ccctgagttt tggcacagac ctgatggctt gagcctgctg atgtccagtg ttctggaaat
      541 ccagtgccca tagatgtgga ctaaggaaga gcccggccat ggtcactctc atcacagaga
      601 agctgcagaa ccagagtttg gatgacctca cccgcagggc ctgtgaggct ggcccgtatt
      661 ctgctgagaa gctgaacaaa agtggccatt tgttcccttt ggagatcagc gtggataaaa
      721 gtccctggaa ggccttacgt ggaggatggc ccactggaag ccaggcagcc tcaggcccct
      781 tctcagtggg cccacatggt gtgtctcaca ccgaaggtct caagtggcag ctggaatccc
      841 cagggcccat ggatgtgggc catttcctgg acctccatga tagcacaggg ccacctgcag
      901 caccaccaac caagcgacac tgccggtccc tgtcagaacc agaggagcta gctcgatgcc
      961 gctccccgtg gcgccctggt agctccaagg tgtggactcc catctccaag aggaggtgca
     1021 acagtggtgg gagtgccaca cttcagtgct gcagcggcgt gggcaaccct actctgcagg
     1081 gcactctagt ccctggccta cccaggaggc ctgtgtcacc cgctggccct acctcacccc
     1141 tgacgccccg gccagcttca gccagcagtg gctttgtgga tggcagtgag ggcagtacaa
     1201 gctcaggccc accctggctt tctacaggac cctgcccatt ttcttccagg cgccgactgt
     1261 ccctctcaca agagcacctt gtagatacag gtgcttgcct gccttcagcc agcagcaccc
     1321 ccacttccac acctgagcta ggccggcacc atggcctgct ccggtgccgc tcacagcctt
     1381 gtgtattgga tgggaggagg gtccggcgca agcgaagacg ggaggaggat gccaggtgga
     1441 cacgcccgtc cttggacttt ctgaaaatga cacggacttt aaaaaactcc aagagccttt
     1501 gctccctgga ctatgaagac gatgaggatg acacccagga gaagactctt gtgtcctccc
     1561 cgtgtaactc acagggcctc gtgggcatca tcacaccgag ctctagccca aggattccca
     1621 ggcctggccc tgacagcccc agtatctggg cgtcagggga gccagaggcc aacccaggcg
     1681 agggtgggag cagtggggac cccagtgact gggacagtgc aggagaggag ggcatcttcc
     1741 cactggacca cggcgacctg gacttggagc agattgagaa caactgactt tggctgagca
     1801 ggatgactgt ggttgctgtc tgtcaccttg gtgcctgacc agtggaaata acttgtgtgt
     1861 tattttgaaa attgaggcgt aattttgatt cagtttttcc taaagaagta ttttgcattt
     1921 ttatggcttc tacagtttga gagagcttct ctattttgaa atgggtttca gagggcattc
     1981 tattaaacat gattctgcct agaaaaaaaa aaaaaaaaaa aaaaaaaa
//
