LOCUS       NM_183254                745 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus family with sequence similarity 229, member B
            (Fam229b), transcript variant 1, mRNA.
ACCESSION   NM_183254
VERSION     NM_183254.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Sun,J., Lu,Y., Nozawa,K., Xu,Z., Morohoshi,A., Castaneda,J.M.,
            Noda,T., Miyata,H., Abbasi,F., Shawki,H.H., Takahashi,S.,
            Devlin,D.J., Yu,Z., Matzuk,R.M., Garcia,T.X., Matzuk,M.M. and
            Ikawa,M.
  TITLE     CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or
            epididymis-enriched genes individually dispensable for male
            reproductiondagger
  JOURNAL   Biol Reprod 103 (2), 183-194 (2020)
   PUBMED   32588039
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153958.2, AK006353.1 and CX567331.1.
            
            On Jan 4, 2018 this sequence version replaced NM_183254.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CA466283.1, AK006353.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AC153958.2         123825-123835       c
            12-707              AK006353.1         2-697
            708-744             CX567331.1         531-567
            745-745             AC153958.2         108733-108733       c
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 20.14 cM"
     gene            1..745
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /note="family with sequence similarity 229, member B"
                     /db_xref="GeneID:66337"
                     /db_xref="MGI:MGI:1913587"
     exon            1..84
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            85..216
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            217..375
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            376..514
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             390..632
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0731 protein C6orf225 homolog; protein FAM229B"
                     /codon_start=1
                     /product="protein FAM229B isoform 1"
                     /protein_id="NP_899077.1"
                     /db_xref="CCDS:CCDS23786.1"
                     /db_xref="GeneID:66337"
                     /db_xref="MGI:MGI:1913587"
                     /translation="MPFRFGTQPRRFPVEGGDSSIELESGLSSSASCTGKETSPNRQL
                     RRCPGSHCLTITDVPITVYATMRKPPAQSSKEMHPK"
     exon            515..745
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      712..717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      745
                     /gene="Fam229b"
                     /gene_synonym="1700025K23Rik"
                     /note="major polyA site"
ORIGIN      
        1 gttaccatag cgactgggcc tccggcaggt aaatcctagc tgccctgtca acatccttga
       61 gcatctgcag ctccggaggc cctgagctct gcccatgctg tagttcctgt cattctccag
      121 aagccacgca acagcaagga gttgtaagaa agtccttatg agtttcaccg ataacttcag
      181 tctgcagtga atcagatgtg tctgcttcac agccagcagg caggaagcta tgtgaaggaa
      241 tcttctgatg tcataattct gggtgtcact ggaacatttg atcaacattc ctttggcaag
      301 tctagccttc tatggaaagt cagtagacgc aattgattta ttcacctcta caggaatcag
      361 acttggccta ttttggattt cagtgaatta tgccttttcg gtttgggacc cagccaagga
      421 ggtttccagt ggaaggagga gactcttcaa ttgagctaga atcaggcctg agctctagtg
      481 cttcctgtac cgggaaagag acgtcaccca ataggcaact ccgaagatgc cctggaagtc
      541 actgcctgac aataactgat gttcccatca ctgtctatgc aacaatgaga aagccacctg
      601 cgcaaagcag caaggaaatg catcccaaat agcatcatta agtcttctgt cgagggttga
      661 ctaggtcaag ggtaatgggc cagtatcatc tcacggtctg cctggtaaac aaataaaggt
      721 ggtggcactc attgggtgat gacaa
//
