LOCUS       NM_133703               2190 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus family with sequence similarity 220, member A
            (Fam220a), transcript variant 2, mRNA.
ACCESSION   NM_133703
VERSION     NM_133703.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wiese,C.B., Zhong,J., Xu,Z.Q., Zhang,Y., Ramirez Solano,M.A.,
            Zhu,W., Linton,M.F., Sheng,Q., Kon,V. and Vickers,K.C.
  TITLE     Dual inhibition of endothelial miR-92a-3p and miR-489-3p reduces
            renal injury-associated atherosclerosis
  JOURNAL   Atherosclerosis 282, 121-131 (2019)
   PUBMED   30731284
  REMARK    GeneRIF: Tgfb2 and Fam220a were identified as putative targets of
            miR-489-3p and miR-92a-3p, respectively; inhibition endothelial
            miR-92a-3p and miR-489-3p reduces renal injury-associated
            atherosclerosis
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Ren,F., Geng,Y., Minami,T., Qiu,Y., Feng,Y., Liu,C., Zhao,J.,
            Wang,Y., Fan,X., Wang,Y., Li,M., Li,J. and Chang,Z.
  TITLE     Nuclear termination of STAT3 signaling through SIPAR
            (STAT3-Interacting Protein As a Repressor)-dependent recruitment of
            T cell tyrosine phosphatase TC-PTP
  JOURNAL   FEBS Lett 589 (15), 1890-1896 (2015)
   PUBMED   26026268
  REMARK    GeneRIF: results suggest that SIPAR terminates the activation of
            STAT3 through a dephosphorylation process that is dependent upon
            interaction with TC45 in the nucleus
REFERENCE   3  (bases 1 to 2190)
  AUTHORS   Ren,F., Su,F., Ning,H., Wang,Y., Geng,Y., Feng,Y., Wang,Y.,
            Zhang,Y., Jin,Z., Li,Y., Jia,B. and Chang,Z.
  TITLE     SIPAR negatively regulates STAT3 signaling and inhibits progression
            of melanoma
  JOURNAL   Cell Signal 25 (11), 2272-2280 (2013)
   PUBMED   23917203
  REMARK    GeneRIF: SIPAR, functioning as a new negative regulator, inhibits
            STAT3 activity by enhancing its dephosphorylation and represses
            melanoma progression.
REFERENCE   4  (bases 1 to 2190)
  AUTHORS   Ueda,T., Manabe,H., Tokuhiro,K., Hirose,M., Matsuoka,Y.,
            Miyagawa,Y., Tsujimura,A., Fujita,K., Wada,M., Okuyama,A.,
            Nishimune,Y. and Tanaka,H.
  TITLE     Unique alternative translation from two open reading frames on
            Acpin1 mRNA yields an acrosomal protein and a
            salivary-gland-specific protein
  JOURNAL   Int J Urol 16 (7), 639-646 (2009)
   PUBMED   19659802
  REMARK    GeneRIF: The two ORFs on the Acpin1 mRNA are independently
            translated in differentiated cells
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC217112.7.
            
            On Jan 31, 2019 this sequence version replaced NM_133703.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK075875.1, AK076127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            bicistronic transcript :: PMID: 19659802
            RefSeq Select criteria :: based on manual assertion, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-415               AC217112.7         179404-179818
            416-2190            AC217112.7         193441-195215
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 82.27 cM"
     gene            1..2190
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /note="family with sequence similarity 220, member A"
                     /db_xref="GeneID:67238"
                     /db_xref="MGI:MGI:1914488"
     exon            1..415
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /inference="alignment:Splign:2.1.0"
     exon            416..2190
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /inference="alignment:Splign:2.1.0"
     CDS             494..1276
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /note="acrosomal protein ACPIN1; STAT3-interacting protein
                     as a repressor"
                     /codon_start=1
                     /product="protein FAM220A"
                     /protein_id="NP_598464.3"
                     /db_xref="CCDS:CCDS51690.1"
                     /db_xref="GeneID:67238"
                     /db_xref="MGI:MGI:1914488"
                     /translation="MKAGRGTLGVCLAKQSQGGDPDKLACGLKKRSQKRNPSPSVVPS
                     WTDQPVADSHGKSRATGAAASEMKHGQSKASLLHHGGFKVLQSLKGSVGRSSAPAASL
                     GKAVALSPAPSEEQLAGMSHGIGDALGSDWPGREPRATDNRGQYLKGESWVSGRPGHP
                     KLREVGFLRGEPPSAGPKGLGTWSELSHRYFELGQLPYAYPYYKVLPEGELRCVSLDR
                     FNPGLSEETVEDEKTLKFFRWSADSRGVTGSAIFQISKSLMP"
     regulatory      1984..1989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /note="hexamer: AATAAA"
     polyA_site      2007
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
     polyA_site      2052
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /note="major polyA site"
     regulatory      2163..2168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /note="hexamer: AATAAA"
     polyA_site      2190
                     /gene="Fam220a"
                     /gene_synonym="1500026F15Rik; 2810453I06Rik; Acpin1;
                     Sipar"
                     /note="major polyA site"
ORIGIN      
        1 aggccgcgcg gcccggccgg gaatccaggt ggtagctggt ggagtcgcct ccggagagtg
       61 acgcgcagac tcggctcccc cgcggcccgc cctcctgccg gcctcgccgc ggtctccctt
      121 gctccctgag atcgctgagc gctgagcagc ggcccgggag aggaggcctt gggcgacggg
      181 gcgcggagag ggagggcggg cgggcagtgg gggcgccgcg gatctctata tggcgacggc
      241 tctgtcgggt ctggctgtcc ggctgtcgcg ctcggccgcc gcccgctcct atggggtctt
      301 ctgcaagggg ctgacccgca cgctgctcat cttctttgac ttggcttggc ggctgcgcat
      361 caactttcct tacctctaca tcgtggcttc catgatgctc aacgtgcgcc tgcaggttca
      421 cattgagatc cactgaaggc catccttgac tgataggcct caccccgact ccctgcatca
      481 aagggcacag agcatgaagg ctgggagagg gaccctgggt gtctgcctgg ccaaacagtc
      541 acaaggaggt gacccggaca aactagcatg tggccttaag aaaagaagtc agaagaggaa
      601 ccccagccca tcagttgtgc cttcttggac agaccagcct gtagcagata gccatggaaa
      661 gtcacgggcc acgggggcag cagcctcaga aatgaagcat ggtcagagca aggccagcct
      721 cctgcatcat ggtggcttca aagtgcttca gtctctgaag ggatcagtgg ggaggagttc
      781 agccccagca gcctccctcg gcaaggctgt ggctctgtcc cctgctcctt cagaagagca
      841 actagccggg atgtcccatg gcatcgggga tgcactagga agtgactggc caggaagaga
      901 gcccagagcc acagacaacc gtggacagta cctcaaagga gagtcctggg tgtcaggacg
      961 gccaggccat ccaaaactga gggaagtggg attcctaaga ggtgaaccac caagtgctgg
     1021 ccccaagggg ctaggcacct ggtcagaact gtctcaccga tactttgagc tgggccagct
     1081 gccatatgct tacccctatt ataaagttct cccagaaggc gagctgaggt gtgtgtccct
     1141 tgaccgtttc aatcccgggc tttcagagga aacagtagaa gatgagaaaa ctctcaagtt
     1201 cttcagatgg tccgcagata gtcgtggggt cactggctct gcaatcttcc agattagcaa
     1261 gtctctgatg ccatagttaa ggttcaagca gaacaagaaa aatagcagat taactttaat
     1321 aaaggttgtc ttgcagtttc ttttgcatta agtctggcgt atgctgcagc cgttcagttc
     1381 ctgttgtttc ctgtgaacca gacgcacctc ggtggttgat gcagaggggg acgttttcga
     1441 gcatgttctc aatcgtttcg gaaagacgct cagaactggg aaccccccct cccccaggaa
     1501 aatgattgat gtgctccgta taagatcaga caggaacctt tatcacagag ttctaaatca
     1561 tggagaccca tcctcaaggg ctagcgtccg gatgccgcct gcctccgctc acctctggcc
     1621 atctgatgga agtggaggcc tgtaggctac gccttccact tcttgtaagc cttgcttcag
     1681 tcatgaagag tcttgcttgc catttctgca ctgtactgac tcggccagag gtcccttctc
     1741 acgtgtcccc ctccttccgc ccgagttgca gtttttaatg gaggcacttt ggagaagttg
     1801 caatattaaa ttgaggataa tttacaatta cagaagagtc tgaagctttc ctgccgacag
     1861 aacacagaca gcaaacgaag caatgaagca ttttccttta taccggccca ggacttgtga
     1921 tttcttttac attaagattt tcttccaaag tgaccctccc tccttctgtg gtttggtaac
     1981 tttaataaag gtcatttaaa gagtggacat tttacagcct ccctacccaa gacagagtta
     2041 aaaacagcca tgggagagtt gggggaccct gactgtatat gtcatttata ttgtgtccta
     2101 aattactatg ccatgggcta ttttagtgtt ttgggaaaat aataaaatct gttctttagc
     2161 ataataaata tgttttattt tgtgtgtgtg
//
