LOCUS       NM_001418036            1557 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus FAM20B, glycosaminoglycan xylosylkinase (Fam20b),
            transcript variant 5, mRNA.
ACCESSION   NM_001418036
VERSION     NM_001418036.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Chen,X., Li,N., Hu,P., Li,L., Li,D., Liu,H., Zhu,L., Xiao,J. and
            Liu,C.
  TITLE     Deficiency of Fam20b-Catalyzed Glycosaminoglycan Chain Synthesis in
            Neural Crest Leads to Cleft Palate
  JOURNAL   Int J Mol Sci 24 (11), 9634 (2023)
   PUBMED   37298583
  REMARK    GeneRIF: Deficiency of Fam20b-Catalyzed Glycosaminoglycan Chain
            Synthesis in Neural Crest Leads to Cleft Palate.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Rios,J.J., Denton,K., Russell,J., Kozlitina,J., Ferreira,C.R.,
            Lewanda,A.F., Mayfield,J.E., Moresco,E., Ludwig,S., Tang,M., Li,X.,
            Lyon,S., Khanshour,A., Paria,N., Khalid,A., Li,Y., Xie,X.,
            Feng,J.Q., Xu,Q., Lu,Y., Hammer,R.E., Wise,C.A. and Beutler,B.
  TITLE     Germline Saturation Mutagenesis Induces Skeletal Phenotypes in Mice
  JOURNAL   J Bone Miner Res 36 (8), 1548-1565 (2021)
   PUBMED   33905568
REFERENCE   3  (bases 1 to 1557)
  AUTHORS   Wu,J., Tian,Y., Han,L., Liu,C., Sun,T., Li,L., Yu,Y.,
            Lamichhane,B., D'Souza,R.N., Millar,S.E., Krumlauf,R., Ornitz,D.M.,
            Feng,J.Q., Klein,O., Zhao,H., Zhang,F., Linhardt,R.J. and Wang,X.
  TITLE     FAM20B-catalyzed glycosaminoglycans control murine tooth number by
            restricting FGFR2b signaling
  JOURNAL   BMC Biol 18 (1), 87 (2020)
   PUBMED   32664967
  REMARK    GeneRIF: FAM20B-catalyzed glycosaminoglycans control murine tooth
            number by restricting FGFR2b signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1557)
  AUTHORS   Wu,J., Li,H., Han,L., Sun,T., Tian,Y. and Wang,X.
  TITLE     The spatiotemporal expression pattern of Syndecans in murine
            embryonic teeth
  JOURNAL   Gene Expr Patterns 36, 119109 (2020)
   PUBMED   32220631
REFERENCE   5  (bases 1 to 1557)
  AUTHORS   Saiyin,W., Li,L., Zhang,H., Lu,Y. and Qin,C.
  TITLE     Inactivation of FAM20B causes cell fate changes in annulus fibrosus
            of mouse intervertebral disc and disc defects via the alterations
            of TGF-beta and MAPK signaling pathways
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1865 (12), 165555 (2019)
   PUBMED   31513834
  REMARK    GeneRIF: These results indicate that FAM20B plays an essential role
            in the development of annulus fibrosus by regulating the TGF-beta
            signaling and MAPK signaling pathways.
REFERENCE   6  (bases 1 to 1557)
  AUTHORS   O'Sullivan,J., Bitu,C.C., Daly,S.B., Urquhart,J.E., Barron,M.J.,
            Bhaskar,S.S., Martelli-Junior,H., dos Santos Neto,P.E.,
            Mansilla,M.A., Murray,J.C., Coletta,R.D., Black,G.C. and Dixon,M.J.
  TITLE     Whole-Exome sequencing identifies FAM20A mutations as a cause of
            amelogenesis imperfecta and gingival hyperplasia syndrome
  JOURNAL   Am J Hum Genet 88 (5), 616-620 (2011)
   PUBMED   21549343
REFERENCE   7  (bases 1 to 1557)
  AUTHORS   Watanabe,Y., Takeuchi,K., Higa Onaga,S., Sato,M., Tsujita,M.,
            Abe,M., Natsume,R., Li,M., Furuichi,T., Saeki,M., Izumikawa,T.,
            Hasegawa,A., Yokoyama,M., Ikegawa,S., Sakimura,K., Amizuka,N.,
            Kitagawa,H. and Igarashi,M.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required
            for normal cartilage development
  JOURNAL   Biochem J 432 (1), 47-55 (2010)
   PUBMED   20812917
REFERENCE   8  (bases 1 to 1557)
  AUTHORS   Nalbant,D., Youn,H., Nalbant,S.I., Sharma,S., Cobos,E., Beale,E.G.,
            Du,Y. and Williams,S.C.
  TITLE     FAM20: an evolutionarily conserved family of secreted proteins
            expressed in hematopoietic cells
  JOURNAL   BMC Genomics 6, 11 (2005)
   PUBMED   15676076
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1557)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 1557)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC145078.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253012.1579973.1,
                                           SRR13422600.1281440.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               AC145078.2         140536-140735       c
            201-700             AC145078.2         127338-127837       c
            701-787             AC145078.2         124069-124155       c
            788-897             AC145078.2         120397-120506       c
            898-1069            AC145078.2         112147-112318       c
            1070-1261           AC145078.2         109026-109217       c
            1262-1557           AC145078.2         107625-107920       c
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 67.71 cM"
     gene            1..1557
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /note="FAM20B, glycosaminoglycan xylosylkinase"
                     /db_xref="GeneID:215015"
                     /db_xref="MGI:MGI:2443990"
     exon            1..200
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     exon            201..700
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..1325
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /note="isoform b is encoded by transcript variant 5;
                     glycosaminoglycan xylosylkinase; xylose kinase; family
                     with sequence similarity 20, member B"
                     /codon_start=1
                     /product="glycosaminoglycan xylosylkinase isoform b"
                     /protein_id="NP_001404965.1"
                     /db_xref="GeneID:215015"
                     /db_xref="MGI:MGI:2443990"
                     /translation="MKLKQRVVLLAILLVIFIFTKVFLIDNLDTSAANREDQRAFHRM
                     MTGLRVELVPKLDHTLQSPWEIAAQWVVPREVYPEETPELGAIMHAMATKKIIKADVG
                     YKGTQLKALLILEGGQKVVFKPKRYSRDYVVEGEPYAGYDRHNAEVAAFHLDRILGFR
                     RAPLVVGRYVNLRTEVKPVATEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDMME
                     GSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDII
                     DTAVFDYLIGNADRHHYESFQDDEGASMLILLDNAKSFGNPSLDERSILAPLYQCCM"
     exon            701..787
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     exon            788..897
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1069
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1261
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1557
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1539..1544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /note="hexamer: AATAAT"
     polyA_site      1557
                     /gene="Fam20b"
                     /gene_synonym="C530043G21Rik; mKIAA0475"
                     /note="major polyA site"
ORIGIN      
        1 gtggagcggc cggctccgcg gggagcggcg accggggaat ggccgggccg gcggtgggcg
       61 ggagccgcag tggtggcggc ggcggcccgg ggcggtgagt gcggcgtggg gctgcccctc
      121 cccggaggcg gcgggggcgg ccggggccgc gcctcaccgc accgcgcggg cggctatgga
      181 gcgagcctga ggcccgccag gaattgtgga ggcactgcag tgaagaaata tgtaatcatg
      241 gaatctcctt gctaatcgtc accacctgtt ccctgtgata agccagccag gacgtgggct
      301 gaggagaagg aaaagaggcc accatgaagc tgaagcagcg agttgtgctg ttagcaatac
      361 tcctcgtcat ttttatcttc accaaagttt tcctgataga caatttagat acatcagctg
      421 ccaaccgaga ggaccagagg gcttttcacc gaatgatgac tggcttgcgg gtggagctgg
      481 tgcccaagtt ggaccatacc ctgcagtctc cttgggagat tgcagcccag tgggtggtgc
      541 cccgggaagt gtatcctgaa gagacaccag agctgggagc aatcatgcat gccatggcca
      601 ctaagaaaat cattaaagct gacgtgggct ataaagggac acagctaaaa gctttactga
      661 ttcttgaagg aggacagaaa gttgtcttta agcctaagcg gtacagcaga gactatgtgg
      721 tagaagggga gccatacgct ggttatgaca gacacaatgc agaggtggcg gccttccatt
      781 tggacaggat tctgggtttc cgccgcgctc ccttggtggt gggcagatat gttaatctgc
      841 ggacagaagt taagcctgtt gccacggagc agctgctgag caccttccta actgtaggga
      901 acaatacttg tttctatggg aagtgctact actgccgaga aacagagcca gcatgtgctg
      961 acggtgacat gatggagggc tctgtcacac tttggcttcc agatgtgtgg cctctgcaga
     1021 aacatcgaca tccctggggc aggacctacc gagaaggcaa actggccagg tgggaatatg
     1081 atgagagcta ctgcgatgct gtgaagaaaa catcccccta tgactcaggc ccgcgtctcc
     1141 tggacatcat tgacacggct gtctttgatt acttgattgg caatgctgat cgccatcact
     1201 acgagagctt tcaagatgat gaaggcgcga gcatgcttat tcttcttgat aatgccaaaa
     1261 gctttggaaa cccctcgctg gatgagagaa gcattcttgc ccctctctat cagtgttgca
     1321 tgtaagttat gtacagcgtg tgtctgcctg catttcctcc ttcaggatca gtacatttgc
     1381 agcagttttc ttgaccttaa aacgtcccca ttgcatttcc tgcttctgta gcccagagcc
     1441 cctgtgaacc ctgacagggg tgagggaaac acagatgcag gccacccaag actcagaatt
     1501 tgttttgaat tttgcatcag taagtctgta gtatctataa taatatctat aataata
//
