LOCUS       NM_178086               2492 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus fatty acid 2-hydroxylase (Fa2h), mRNA.
ACCESSION   NM_178086
VERSION     NM_178086.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Zhou,X., Huang,F., Ma,G., Wei,W., Wu,N. and Liu,Z.
  TITLE     Dysregulated ceramides metabolism by fatty acid 2-hydroxylase
            exposes a metabolic vulnerability to target cancer metastasis
  JOURNAL   Signal Transduct Target Ther 7 (1), 370 (2022)
   PUBMED   36274060
  REMARK    GeneRIF: Dysregulated ceramides metabolism by fatty acid
            2-hydroxylase exposes a metabolic vulnerability to target cancer
            metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2492)
  AUTHORS   Jordans,S., Hardt,R., Becker,I., Winter,D., Wang-Eckhardt,L. and
            Eckhardt,M.
  TITLE     Age-Dependent Increase in Schmidt-Lanterman Incisures and a
            Cadm4-Associated Membrane Skeletal Complex in Fatty Acid
            2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia
            SPG35
  JOURNAL   Mol Neurobiol 59 (7), 3969-3979 (2022)
   PUBMED   35445918
  REMARK    GeneRIF: Age-Dependent Increase in Schmidt-Lanterman Incisures and
            a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid
            2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia
            SPG35.
REFERENCE   4  (bases 1 to 2492)
  AUTHORS   Ji,Y., Luo,Z., Gao,H., Dos Reis,F.C.G., Bandyopadhyay,G., Jin,Z.,
            Manda,K.A., Isaac,R., Yang,M., Fu,W., Ying,W. and Olefsky,J.M.
  TITLE     Hepatocyte-derived exosomes from early onset obese mice promote
            insulin sensitivity through miR-3075
  JOURNAL   Nat Metab 3 (9), 1163-1174 (2021)
   PUBMED   34489604
REFERENCE   5  (bases 1 to 2492)
  AUTHORS   Hardt,R., Jordans,S., Winter,D., Gieselmann,V., Wang-Eckhardt,L.
            and Eckhardt,M.
  TITLE     Decreased turnover of the CNS myelin protein Opalin in a mouse
            model of hereditary spastic paraplegia 35
  JOURNAL   Hum Mol Genet 29 (22), 3616-3630 (2021)
   PUBMED   33215680
  REMARK    GeneRIF: Decreased turnover of the CNS myelin protein Opalin in a
            mouse model of hereditary spastic paraplegia 35.
REFERENCE   6  (bases 1 to 2492)
  AUTHORS   Zoller,I., Meixner,M., Hartmann,D., Bussow,H., Meyer,R.,
            Gieselmann,V. and Eckhardt,M.
  TITLE     Absence of 2-hydroxylated sphingolipids is compatible with normal
            neural development but causes late-onset axon and myelin sheath
            degeneration
  JOURNAL   J Neurosci 28 (39), 9741-9754 (2008)
   PUBMED   18815260
REFERENCE   7  (bases 1 to 2492)
  AUTHORS   Alderson,N.L., Maldonado,E.N., Kern,M.J., Bhat,N.R. and Hama,H.
  TITLE     FA2H-dependent fatty acid 2-hydroxylation in postnatal mouse brain
  JOURNAL   J Lipid Res 47 (12), 2772-2780 (2006)
   PUBMED   16998236
  REMARK    GeneRIF: FA2H is the major fatty acid 2-hydroxylase in brain, and
            2-hydroxylation of free fatty acids is the first step in the
            synthesis of 2-hydroxy galactolipids
REFERENCE   8  (bases 1 to 2492)
  AUTHORS   Eckhardt,M., Yaghootfam,A., Fewou,S.N., Zoller,I. and Gieselmann,V.
  TITLE     A mammalian fatty acid hydroxylase responsible for the formation of
            alpha-hydroxylated galactosylceramide in myelin
  JOURNAL   Biochem J 388 (Pt 1), 245-254 (2005)
   PUBMED   15658937
  REMARK    GeneRIF: FA2H is the enzyme responsible for the formation of
            alpha-hydroxylated ceramide in oligodendrocytes of the mammalian
            brain.
REFERENCE   9  (bases 1 to 2492)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2492)
  AUTHORS   Charles,C.H., Abler,A.S. and Lau,L.F.
  TITLE     cDNA sequence of a growth factor-inducible immediate early gene and
            characterization of its encoded protein
  JOURNAL   Oncogene 7 (1), 187-190 (1992)
   PUBMED   1741163
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ152901.1, AK161502.1 and BB076849.1.
            
            On Oct 20, 2007 this sequence version replaced NM_178086.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK161502.1, AK090338.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 CJ152901.1         4-6
            4-1853              AK161502.1         2-1851
            1854-2489           AK161502.1         1853-2488
            2490-2492           BB076849.1         247-249
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.98 cM"
     gene            1..2492
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /note="fatty acid 2-hydroxylase"
                     /db_xref="GeneID:338521"
                     /db_xref="MGI:MGI:2443327"
     exon            1..339
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..1188
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /note="fatty acid hydroxylase domain containing 1; fatty
                     acid alpha-hydroxylase"
                     /codon_start=1
                     /product="fatty acid 2-hydroxylase"
                     /protein_id="NP_835187.2"
                     /db_xref="CCDS:CCDS22674.1"
                     /db_xref="GeneID:338521"
                     /db_xref="MGI:MGI:2443327"
                     /translation="MAPAPPPAASFTPAEVQRRLAAGACWVRRGASLYDLTSFVRHHP
                     GGEQLLLARAGQDISADLDGPPHRHSDNARRWLEQYYVGELRADPQDPTENGAVASAE
                     TQKTDPALEPQFKVVDWDKDLVDWQKPLLWQVGHLGEKYDEWVHQPVARPIRLFHSDL
                     IEAFSKTVWYSVPIIWVPLVLYLSWSYYRTLTQDNIRLFASLTREYSMMMPESVFIGL
                     FVLGMLFWTFVEYVIHRFLFHMKPPSNSHYLIMLHFVMHGQHHKAPFDGSRLVFPPVP
                     ASLVIAFFYVFLRLILPETVGGIIFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYNMKA
                     HHVKHHFEYQKSGFGISTKLWDYFFHTLIPEEAHPKMQ"
     exon            340..432
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            433..575
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            576..682
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            683..855
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1108
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..2492
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2468..2473
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2490
                     /gene="Fa2h"
                     /gene_synonym="FAAH; Faxdc1; G630055L08Rik"
                     /note="major polyA site"
ORIGIN      
        1 gttagaggcg cgctgggcag ccgcggagca gactgccggg ctccgagatc cgagctccgc
       61 tctcccgcca tggcccccgc tccgcccccc gccgcctctt tcacgcccgc cgaggtccag
      121 cggcgcctgg cggccggtgc ctgctgggtc cgccgcgggg ccagcctcta cgacctcacc
      181 agcttcgtgc ggcatcatcc ggggggcgag cagctgctgc tggcgcgggc tggccaggac
      241 atcagtgccg acctggatgg gccacctcac agacactccg acaacgcccg ccgctggctg
      301 gagcagtact atgtgggcga actgcgagcg gacccgcagg atcccacaga gaatggtgct
      361 gtagcctctg cagagactca gaagacagac cctgctcttg agccacagtt caaagtggtg
      421 gactgggaca aggacctggt ggactggcag aaacctctgc tgtggcaggt gggccacttg
      481 ggggagaagt atgatgagtg ggttcaccaa ccagtggcca ggcccattcg cctcttccac
      541 tcagacctca tcgaggcctt ctccaagact gtctggtata gtgtccccat catctgggtg
      601 cccctggtgc tgtacctcag ctggtcctac taccgaaccc tcacccagga caacatccgg
      661 ctcttcgcat cactcacaag agagtattca atgatgatgc cggagtcggt gttcataggc
      721 ctctttgtgc tgggcatgct tttttggacc tttgtggaat acgtcatcca ccgcttcctg
      781 ttccatatga agccccccag caacagccat tacctcatca tgttgcattt tgtcatgcac
      841 ggccagcacc acaaggcacc gtttgatggc tcccgtctgg tcttcccccc agtgccagcc
      901 tccctagtga ttgccttctt ctatgtgttc ctgcggctca ttctgcctga gaccgtggga
      961 ggcatcatct ttgctggggg tctcctgggc tatgtcctct atgacatgac acattactac
     1021 ctgcacttcg gctctccaca caagggctcc tacctgtaca acatgaaggc ccaccatgtc
     1081 aagcatcact tcgaatacca gaaatcaggg tttggcatca gcactaaact gtgggattac
     1141 tttttccaca ccctcattcc agaggaagcc cacccgaaga tgcagtgaca acccctagac
     1201 cctctgttct gccctcggtc tatccccaga ccccacccat gtcctacccc ccacctccac
     1261 ctagacctgc tgaggtcagc ttggccaggc atgacgggct ctgtctagcc ctggggcctg
     1321 gtggagacct gaaagcaggg cttcccttcc ggcttcctgt gtgaggatag catcctcttg
     1381 atagccgtgg cccttgggga ctggctcatt cccagagaat gatggcctcc ccgttggcat
     1441 tcctgcctaa gcttcagccc tcagtgctgt ttcagggtcc agacattggc cccaaggggc
     1501 atgaaggaaa ctgaccccac ctgtgcctta gaaccctggg ctccctgggg gcttccccat
     1561 gcagcagagg gaccggtgtc acagcagaag catggcatgc aggcctgtgg ccacatcgta
     1621 ttctcctgtc ctttcttggg agagtttgat gattcagaag taaatgaagg gctattccaa
     1681 gaagaaacag catgatgcct cccacaggcc attcccagac cccaggacag ccactatggg
     1741 ccctgccaac cccagatacc agctgtacta tcagctgcgg aagtaatcca gtacttgtct
     1801 gcctgggcag aaagcacaag ggtgcctggc atcctctgca aggctgacag gagccctggc
     1861 ccttgtcttt ggggatcagc agtggctttc tctgtctcct ctctccctgc ccaaaccaca
     1921 aaaccgctcc ccaacagagg ctggagaagt ctacgttggt gagagcccca gcctagcccc
     1981 ggtcatactt ggcctccatc tgcacaggta gaaacctgcc ccactcagga ccctcccaga
     2041 agtgcctcct gaagccaaga agggacttgt atgtggtgcc ttatcaccca ccctctccca
     2101 cccagactgt ccttccttgt aaccagagcc ctcactgctc ctgagtctta tttataagct
     2161 acataaatat ttttaatctg gccccaaatg aagtagctgg tcagatggcc ctttctaatc
     2221 ctatgtattg agcttatgta aaagacaaaa aaaaatcatc ttgccttcct ttttgttcct
     2281 ttcttaccca caagcgatta ctacttgaaa cttatcaaaa acccaatggg tgcaaagact
     2341 aaagagaggc agtgtgacca gtctatgatt tgtactgttt acggtgaagc tatctagaga
     2401 tttttgggat aaatatacaa aactgcactt gtgaaataaa gatgtaaata aattgtatat
     2461 tttgaaaaat aaaacattga gaagaaacca gc
//
