LOCUS       NM_007977               7522 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus coagulation factor VIII (F8), transcript variant 1,
            mRNA.
ACCESSION   NM_007977
VERSION     NM_007977.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Kumar,S.R.P., Biswas,M., Cao,D., Arisa,S., Munoz-Melero,M.,
            Lam,A.K., Pineros,A.R., Kapur,R., Kaisho,T., Kaufman,R.J., Xiao,W.,
            Shayakhmetov,D.M., Terhorst,C., de Jong,Y.P. and Herzog,R.W.
  TITLE     TLR9-independent CD8+ T cell responses in hepatic AAV gene transfer
            through IL-1R1-MyD88 signaling
  JOURNAL   Mol Ther 32 (2), 325-339 (2024)
   PUBMED   38053332
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Oleshko,O., Vollack-Hesse,N., Tiede,A., Hegermann,J., Curth,U. and
            Werwitzke,S.
  TITLE     von Willebrand factor modulates immune complexes and the recall
            response against factor VIII in a murine hemophilia A model
  JOURNAL   Blood Adv 7 (21), 6771-6781 (2023)
   PUBMED   37756521
  REMARK    GeneRIF: von Willebrand factor modulates immune complexes and the
            recall response against factor VIII in a murine hemophilia A model.
REFERENCE   3  (bases 1 to 7522)
  AUTHORS   Cooke,E.J., Joseph,B.C., Nasamran,C.A., Fisch,K.M. and von
            Drygalski,A.
  TITLE     Maladaptive lymphangiogenesis is associated with synovial iron
            accumulation and delayed clearance in factor VIII-deficient mice
            after induced hemarthrosis
  JOURNAL   J Thromb Haemost 21 (9), 2390-2404 (2023)
   PUBMED   37116753
  REMARK    GeneRIF: Maladaptive lymphangiogenesis is associated with synovial
            iron accumulation and delayed clearance in factor VIII-deficient
            mice after induced hemarthrosis.
REFERENCE   4  (bases 1 to 7522)
  AUTHORS   Batsuli,G., Ito,J., York,E.S., Cox,C., Baldwin,W., Gill,S.,
            Lollar,P. and Meeks,S.L.
  TITLE     Factor VIII antibody immune complexes modulate the humoral response
            to factor VIII in an epitope-dependent manner
  JOURNAL   Front Immunol 14, 1233356 (2023)
   PUBMED   37720212
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7522)
  AUTHORS   Bravo-Iniguez,C.E., Fritz,J.R., Shukla,S., Sarangi,S.,
            Thompson,D.A., Amin,S.G., Tsaava,T., Chaudhry,S., Valentino,S.P.,
            Hoffman,H.B., Imossi,C.W., Addorisio,M.E., Valdes-Ferrer,S.I.,
            Chavan,S.S., Blanc,L., Czura,C.J., Tracey,K.J. and Huston,J.M.
  TITLE     Vagus nerve stimulation primes platelets and reduces bleeding in
            hemophilia A male mice
  JOURNAL   Nat Commun 14 (1), 3122 (2023)
   PUBMED   37264009
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7522)
  AUTHORS   Korhonen,J., Partanen,J., Armstrong,E., Vaahtokari,A., Elenius,K.,
            Jalkanen,M. and Alitalo,K.
  TITLE     Enhanced expression of the tie receptor tyrosine kinase in
            endothelial cells during neovascularization
  JOURNAL   Blood 80 (10), 2548-2555 (1992)
   PUBMED   1384789
REFERENCE   7  (bases 1 to 7522)
  AUTHORS   Levinson,B., Bermingham,J.R. Jr., Metzenberg,A., Kenwrick,S.,
            Chapman,V. and Gitschier,J.
  TITLE     Sequence of the human factor VIII-associated gene is conserved in
            mouse
  JOURNAL   Genomics 13 (3), 862-865 (1992)
   PUBMED   1639415
REFERENCE   8  (bases 1 to 7522)
  AUTHORS   Derry,J.M. and Barnard,P.J.
  TITLE     Physical linkage of the A-raf-1, properdin, synapsin I, and TIMP
            genes on the human and mouse X chromosomes
  JOURNAL   Genomics 12 (4), 632-638 (1992)
   PUBMED   1572636
REFERENCE   9  (bases 1 to 7522)
  AUTHORS   Ramesh,V., Cheng,S.V., Kozak,C.A., Herron,B.J., Shih,V.E.,
            Taylor,B.A. and Gusella,J.F.
  TITLE     Mapping of ornithine aminotransferase gene sequences to mouse
            chromosomes 7, X, and 3
  JOURNAL   Mamm Genome 3 (1), 17-22 (1992)
   PUBMED   1349842
REFERENCE   10 (bases 1 to 7522)
  AUTHORS   Herman,G.E., Faust,C.J., Darlison,M.G. and Barnard,E.A.
  TITLE     Genetic mapping of the mouse X chromosome in the region homologous
            to human Xq27-Xq28
  JOURNAL   Genomics 9 (4), 670-677 (1991)
   PUBMED   1674728
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK051579.1, L05573.1, BC138347.1 and AW489638.1.
            
            On May 29, 2009 this sequence version replaced NM_007977.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1475              AK051579.1         2277-3751
            1476-3284           L05573.1           1474-3282
            3285-7390           BC138347.1         2956-7061
            7391-7522           AW489638.1         1-132               c
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 38.17 cM"
     gene            1..7522
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /note="coagulation factor VIII"
                     /db_xref="GeneID:14069"
                     /db_xref="MGI:MGI:88383"
     exon            1..555
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     CDS             410..7369
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; procoagulant component; Factor VIII"
                     /codon_start=1
                     /product="coagulation factor VIII isoform 1 precursor"
                     /protein_id="NP_032003.2"
                     /db_xref="CCDS:CCDS30238.1"
                     /db_xref="GeneID:14069"
                     /db_xref="MGI:MGI:88383"
                     /translation="MQIALFACFFLSLFNFCSSAIRRYYLGAVELSWNYIQSDLLSVL
                     HTDSRFLPRMSTSFPFNTSIMYKKTVFVEYKDQLFNIAKPRPPWMGLLGPTIWTEVHD
                     TVVITLKNMASHPVSLHAVGVSYWKASEGDEYEDQTSQMEKEDDKVFPGESHTYVWQV
                     LKENGPMASDPPCLTYSYMSHVDLVKDLNSGLIGALLVCKEGSLSKERTQMLYQFVLL
                     FAVFDEGKSWHSETNDSYTQSMDSASARDWPKMHTVNGYVNRSLPGLIGCHRKSVYWH
                     VIGMGTTPEIHSIFLEGHTFFVRNHRQASLEISPITFLTAQTLLIDLGQFLLFCHISS
                     HKHDGMEAYVKVDSCPEESQWQKKNNNEEMEDYDDDLYSEMDMFTLDYDSSPFIQIRS
                     VAKKYPKTWIHYISAEEEDWDYAPSVPTSDNGSYKSQYLSNGPHRIGRKYKKVRFIAY
                     TDETFKTRETIQHESGLLGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHAR
                     RLPRGIKHVKDLPIHPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGL
                     IGPLLICYKESVDQRGNQMMSDKRNVILFSIFDENQSWYITENMQRFLPNAAKTQPQD
                     PGFQASNIMHSINGYVFDSLELTVCLHEVAYWHILSVGAQTDFLSIFFSGYTFKHKMV
                     YEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRKRGMTALLKVSSCDKSTSDYYEE
                     IYEDIPTQLVNENNVIDPRSFFQNTNHPNTRKKKFKDSTIPKNDMEKIEPQFEEIAEM
                     LKVQSVSVSDMLMLLGQSHPTPHGLFLSDGQEAIYEAIHDDHSPNAIDSNEGPSKVTQ
                     LRPESHHSEKIVFTPQPGLQLRSNKSLETTIEVKWKKLGLQVSSLPSNLMTTTILSDN
                     LKATFEKTDSSGFPDMPVHSSSKLSTTAFGKKAYSLVGSHVPLNVSEENSDSNILDST
                     LMYSQESLPRDNILSMENDRLLREKRFHGIALLTKDNTLFKDNVSLMKTNKTYNHSTT
                     NEKLHTESPTSIENSTTDLQDAILKVNSEIQEVTALIHDGTLLGKNSTYLRLNHMLNR
                     TTSTKNKDIFHRKDEDPIPQDEENTIMPFSKMLFLSESSNWFKKTNGNNSLNSEQEHS
                     PKQLVYLMFKKYVKNQSFLSEKNKVTVEQDGFTKNIGLKDMAFPHNMSIFLTTLSNVH
                     ENGRHNQEKNIQEEIEKEALIEEKVVLPQVHEATGSKNFLKDILILGTRQNISLYEVH
                     VPVLQNITSINNSTNTVQIHMEHFFKRRKDKETNSEGLVNKTREMVKNYPSQKNITTQ
                     RSKRALGQFRLSTQWLKTINCSTQCIIKQIDHSKEMKKFITKSSLSDSSVIKSTTQTN
                     SSDSHIVKTSAFPPIDLKRSPFQNKFSHVQASSYIYDFKTKSSRIQESNNFLKETKIN
                     NPSLAILPWNMFIDQGKFTSPGKSNTNSVTYKKRENIIFLKPTLPEESGKIELLPQVS
                     IQEEEILPTETSHGSPGHLNLMKEVFLQKIQGPTKWNKAKRHGESIKGKTESSKNTRS
                     KLLNHHAWDYHYAAQIPKDMWKSKEKSPEIISIKQEDTILSLRPHGNSHSIGANEKQN
                     WPQRETTWVKQGQTQRTCSQIPPVLKRHQRELSAFQSEQEATDYDDAITIETIEDFDI
                     YSEDIKQGPRSFQQKTRHYFIAAVERLWDYGMSTSHVLRNRYQSDNVPQFKKVVFQEF
                     TDGSFSQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYKEDQ
                     RGEEPRRNFVKPNETKIYFWKVQHHMAPTEDEFDCKAWAYFSDVDLERDMHSGLIGPL
                     LICHANTLNPAHGRQVSVQEFALLFTIFDETKSWYFTENVKRNCKTPCNFQMEDPTLK
                     ENYRFHAINGYVMDTLPGLVMAQDQRIRWYLLSMGNNENIQSIHFSGHVFTVRKKEEY
                     KMAVYNLYPGVFETLEMIPSRAGIWRVECLIGEHLQAGMSTLFLVYSKQCQIPLGMAS
                     GSIRDFQITASGHYGQWAPNLARLHYSGSINAWSTKEPFSWIKVDLLAPMIVHGIKTQ
                     GARQKFSSLYISQFIIMYSLDGKKWLSYQGNSTGTLMVFFGNVDSSGIKHNSFNPPII
                     ARYIRLHPTHSSIRSTLRMELMGCDLNSCSIPLGMESKVISDTQITASSYFTNMFATW
                     SPSQARLHLQGRTNAWRPQVNDPKQWLQVDLQKTMKVTGIITQGVKSLFTSMFVKEFL
                     ISSSQDGHHWTQILYNGKVKVFQGNQDSSTPMMNSLDPPLLTRYLRIHPQIWEHQIAL
                     RLEILGCEAQQQY"
     exon            556..677
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            678..800
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            801..1013
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1082
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1199
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1421
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1680
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1852
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..1946
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2161
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2312
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2522
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..5535
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5536..5689
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5690..5899
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            5900..6128
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6129..6311
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6312..6428
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6429..6500
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6501..6586
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6587..6742
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6743..6887
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            6888..7036
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            7037..7213
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     exon            7214..7509
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7472..7477
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /note="hexamer: AATAAA"
     polyA_site      7495
                     /gene="F8"
                     /gene_synonym="Cf-8; Cf8; FVIII"
                     /note="major polyA site"
ORIGIN      
        1 tctagagttt ctttgctaca ggtaccaagg aacagtcttt tagaataggc taggaattta
       61 aatacacctg aacgcccctc ctcagtattc tgttcctttt cttaaggatt caaacttgtt
      121 aggatgcacc cagcaggaaa tgggttaagc cttagctcag ccactcttcc tattccagtt
      181 ttcctgtgcc tgcttcctac tacccaaaag gaagtaatcc tttcagatct gttttgtgct
      241 aatgctactt tcactcacag tagataaact tccagaaaat gctctgcaaa atatttagga
      301 ctttttacta aatcattaca ttttcttttt gttcttaaaa gctaaagtta ttttagagaa
      361 gagttaaatt ttcatttctt tagttgaaca ttttctagta ataaaagcca tgcaaatagc
      421 actcttcgct tgcttctttc tgagcctttt caatttctgc tctagtgcca tcagaagata
      481 ctaccttggt gcagtggaat tgtcctggaa ctatattcag agtgatctgc tcagtgtgct
      541 gcatacagac tcaagatttc ttcctagaat gtcaacatct tttccattca acacctccat
      601 catgtataaa aagactgtgt ttgtagagta caaggaccag cttttcaaca ttgccaagcc
      661 caggccaccc tggatgggtt tgctaggtcc taccatttgg actgaggttc atgacacagt
      721 ggtcattaca cttaaaaaca tggcttctca tcctgtcagt cttcatgctg ttggtgtgtc
      781 ctactggaaa gcttctgagg gagatgaata tgaagatcag acaagccaaa tggagaagga
      841 agatgataaa gttttccctg gtgaaagtca tacttatgtt tggcaagtcc tgaaagagaa
      901 tggtccaatg gcctctgacc ctccatgtct cacttactca tatatgtctc atgtggatct
      961 ggtgaaagat ttgaattcag gcctcattgg agctctgcta gtatgtaaag aaggcagtct
     1021 ctccaaagaa agaacacaga tgttgtacca atttgtactg ctttttgctg tatttgatga
     1081 agggaagagc tggcactcag aaacaaacga ctcttataca cagtctatgg attctgcatc
     1141 tgctagagac tggcctaaaa tgcacacagt caatggctat gtaaacaggt ctcttccagg
     1201 tctgattgga tgccatagga aatcagtcta ctggcacgtg attggaatgg gcaccactcc
     1261 tgaaatacac tcaatattcc tcgaaggtca cacatttttt gtgaggaacc accgtcaagc
     1321 ttcattggag atatcaccaa taactttcct tactgctcaa acactcttga tagatcttgg
     1381 gcagttccta ctattttgtc atatctcttc ccataaacat gatggcatgg aagcttatgt
     1441 caaagtagat agctgccctg aggaatccca atggcaaaag aaaaataata atgaggaaat
     1501 ggaagattat gatgatgatc tttattcaga aatggatatg ttcacattgg attatgacag
     1561 ctctcctttt atccaaattc gctcggttgc taaaaagtac cctaaaactt ggatacatta
     1621 tatttctgct gaggaggaag actgggacta tgcaccttca gttcctacct cggataatgg
     1681 aagttataaa agccagtatc tgagcaatgg tcctcatcgg attggtagga aatataaaaa
     1741 agtcagattt atagcataca cagatgaaac ctttaagact cgtgaaacta ttcagcatga
     1801 atcaggactc ttgggacctt tactttatgg agaagttgga gacacactgt tgattatttt
     1861 taagaatcaa gcaagccgac catataacat ttaccctcat ggaatcactg atgtcagtcc
     1921 tctacatgca aggagattgc caagaggtat aaagcacgtg aaggatttgc caattcatcc
     1981 aggagagata ttcaagtaca agtggacagt tacagtagaa gatggaccaa ctaaatcaga
     2041 tccacggtgc ctgacccgct attattcaag tttcattaac cctgagagag atctagcttc
     2101 aggactgatt ggccctcttc tcatctgcta caaagaatct gtagatcaaa ggggaaacca
     2161 gatgatgtca gacaaaagaa atgtcatcct gttttctata tttgatgaga accaaagctg
     2221 gtacatcaca gagaacatgc aacgcttcct ccccaatgca gctaaaacac agccccagga
     2281 ccctgggttc caggcctcca acatcatgca cagcatcaat ggctatgttt ttgatagctt
     2341 ggagttgaca gtttgtttgc atgaggtggc atactggcac attctcagtg ttggagcaca
     2401 gacagacttc ttatctatct tcttctctgg atatactttc aaacacaaaa tggtctatga
     2461 agatacactt accctgttcc cattctcagg agaaactgtc tttatgtcga tggaaaaccc
     2521 aggtctatgg gtcttggggt gtcataattc agactttcgg aagagaggta tgacagcatt
     2581 gctgaaagtt tctagttgtg acaagagcac tagtgattat tatgaagaaa tatatgaaga
     2641 tattccaaca cagttggtga atgagaacaa tgtcattgat cccagaagct tcttccagaa
     2701 tacaaatcat cctaatacta ggaaaaagaa attcaaagat tccacaattc caaaaaatga
     2761 tatggagaag attgagcctc agtttgaaga gatagcagag atgcttaaag tacagagtgt
     2821 ctcagttagt gacatgttga tgctcttggg acagagtcat cctactccac atggcttatt
     2881 tttatcagat ggccaagaag ccatctatga ggctattcat gatgatcatt caccaaatgc
     2941 aatagacagc aatgaaggcc catctaaagt gacccaactc aggccagaat cccatcacag
     3001 tgagaaaata gtatttactc ctcagcccgg cctccagtta agatccaata aaagtttgga
     3061 gacaactata gaagtaaagt ggaagaaact tggtttgcaa gtttctagtt tgccaagtaa
     3121 tctaatgact acaacaattc tgtcagacaa tttgaaagca acttttgaaa agacagattc
     3181 ttcaggattt ccagatatgc cagttcactc tagtagtaaa ttaagtacta ctgcatttgg
     3241 taagaaagca tattcccttg ttgggtctca tgtaccttta aacgtgagtg aagaaaatag
     3301 tgattccaac atattggatt caactttaat gtatagtcaa gaaagtttac caagagataa
     3361 tatattatca atggagaatg atagattact cagagagaag aggtttcatg gaattgcttt
     3421 attgaccaaa gataatactt tattcaaaga caatgtctcc ttaatgaaaa caaacaaaac
     3481 atataatcat tcaacaacta atgaaaaact acacactgag agcccaacat caattgagaa
     3541 tagtacaaca gacttgcaag atgccatatt aaaggtcaat agtgagattc aagaagtaac
     3601 agctttgatt catgatggaa cacttttagg caaaaattct acatatttga gactaaacca
     3661 tatgctaaat agaactacct caacaaaaaa taaagacata tttcatagaa aagatgaaga
     3721 tcctattcca caagatgaag agaatacaat catgccattt tccaagatgt tgttcttgtc
     3781 agaatcttca aattggttta aaaagaccaa tggaaataat tccttgaact ctgagcaaga
     3841 acatagtcca aagcaattag tatatttaat gtttaaaaaa tatgtaaaaa atcaaagttt
     3901 cttgtcagag aaaaataaag tcacagtaga acaggatgga tttacaaaga acataggact
     3961 taaagacatg gcttttccac ataatatgag catatttctt accactttgt ctaacgtaca
     4021 tgaaaatggt aggcacaatc aagaaaaaaa tattcaggaa gagatagaga aggaagcact
     4081 aattgaagag aaagtagttt tgccccaggt gcacgaagca actggctcta agaatttctt
     4141 gaaagacata ttgatactag gcactaggca aaatataagt ttatatgaag tacatgtacc
     4201 agtacttcaa aacatcacat caataaacaa ttcaacaaat acagtacaga ttcacatgga
     4261 gcatttcttt aaaagaagga aggacaagga aacaaattca gaaggcttgg taaataaaac
     4321 cagagaaatg gtaaaaaact atccaagcca gaagaatatt actactcaac gtagtaaacg
     4381 ggctttggga caattcagac tgtcaactca atggcttaaa accataaact gttcaacaca
     4441 gtgtatcatt aaacagatag accacagcaa ggaaatgaaa aagttcatta ctaaatcttc
     4501 cttatcagat tcttctgtga ttaaaagcac cactcagaca aatagttctg actcacacat
     4561 tgtaaaaaca tcagcatttc caccaataga tctcaaaagg agtccattcc aaaacaaatt
     4621 ttctcatgtt caagcatcat cctacattta tgactttaag acaaaaagtt caagaattca
     4681 agaaagcaat aatttcttaa aagaaaccaa aataaataac ccttctttag ccattctacc
     4741 atggaatatg ttcatagatc aaggaaaatt tacctcccca gggaaaagta acacaaactc
     4801 agtcacatat aagaaacgtg agaacattat tttcttgaaa ccaactttgc ctgaagaatc
     4861 tggcaaaatt gaattgcttc ctcaagtttc cattcaagag gaagaaattt tacctacaga
     4921 aactagccat ggatctcctg gacacttgaa tctcatgaaa gaggtctttc ttcagaaaat
     4981 acaggggcct actaaatgga ataaagcaaa gaggcatgga gaaagtataa aaggtaaaac
     5041 agagagctct aaaaatactc gctcaaaact gctaaatcat catgcttggg attatcatta
     5101 tgctgcacag ataccaaaag atatgtggaa atccaaagag aagtcaccag aaattatatc
     5161 cattaagcaa gaggacacca ttttgtctct gaggcctcat ggaaacagtc attcaatagg
     5221 ggcaaatgag aaacaaaatt ggcctcaaag agaaaccact tgggtaaagc aaggccaaac
     5281 tcaaaggaca tgctctcaaa tcccaccagt gttgaaacga catcaaaggg aacttagtgc
     5341 ttttcaatca gaacaagaag caactgacta tgatgatgcc atcaccattg aaacaatcga
     5401 ggattttgac atttacagtg aggacataaa gcaaggtccc cgcagctttc aacagaaaac
     5461 aaggcactat tttattgcag ctgtggaacg actctgggac tatgggatga gtacatctca
     5521 tgttctacga aataggtatc aaagtgacaa tgtacctcag ttcaagaaag tagttttcca
     5581 ggaatttact gatggctcct ttagtcagcc cttatatcgt ggagaattaa atgaacacct
     5641 ggggttgttg ggcccatata taagagcaga agttgaagac aacattatgg taactttcaa
     5701 aaaccaggcc tcccgtccct actccttcta ttctagcctc atttcttata aagaagatca
     5761 gagaggagaa gaacctagaa gaaactttgt caagcctaat gaaaccaaaa tttatttttg
     5821 gaaagtacaa catcatatgg cacccacaga agatgagttt gactgcaagg cctgggctta
     5881 tttctctgat gttgatcttg aaagagatat gcactcggga ttaattggac cccttctgat
     5941 ttgccacgcg aacacactga atcctgctca tgggagacaa gtgtcagtac aggaatttgc
     6001 tctgcttttc actatctttg atgagaccaa gagctggtac ttcactgaaa acgtgaaaag
     6061 gaactgcaag acaccctgca atttccagat ggaagacccc actttgaaag agaattatcg
     6121 cttccatgca atcaatggtt atgtaatgga taccctacca ggcttagtaa tggctcaaga
     6181 tcaaaggatt cgatggtatc ttctcagcat gggcaacaat gagaacatcc aatctattca
     6241 tttcagtgga catgttttca ctgtacggaa aaaagaggag tataaaatgg cagtgtacaa
     6301 cctctaccca ggtgtttttg agactctgga aatgatacca tccagagctg gaatatggcg
     6361 agtagaatgc cttattggcg agcacttaca ggctgggatg agcactcttt ttctggtgta
     6421 cagcaagcag tgtcagattc ctcttggaat ggcttctgga agcatccgtg atttccagat
     6481 tacagcttca ggacattatg gacagtgggc cccaaacctg gcaagacttc attattccgg
     6541 atcaatcaat gcctggagta ccaaggagcc cttttcttgg atcaaggtag atctgttggc
     6601 accaatgatt gttcatggca tcaagactca gggtgctcgt cagaaatttt ccagccttta
     6661 tatctctcaa tttatcatca tgtatagcct ggatgggaag aagtggctga gttatcaagg
     6721 aaattccact ggaaccttaa tggttttctt tggcaatgtg gactcatctg ggattaagca
     6781 taatagtttt aatcctccaa ttattgctcg atatatccgt ttgcacccca ctcattctag
     6841 catccgtagt actcttcgca tggagttgat gggctgtgat ttaaacagtt gcagcatacc
     6901 attgggaatg gaaagtaaag taatatcaga tacacaaatc actgcctcat cctacttcac
     6961 caacatgttt gctacttggt ctccttcaca agctcgactt cacctccagg gaaggactaa
     7021 tgcctggcga cctcaggtga atgatccaaa acaatggttg caagtggact tacaaaagac
     7081 aatgaaagtc actggaataa taacccaggg agtgaaatct ctctttacca gcatgtttgt
     7141 gaaagagttc cttatttcca gcagtcaaga tggccatcac tggactcaaa ttttatacaa
     7201 tggcaaggta aaggtttttc aggggaatca ggactcatcc acacctatga tgaattctct
     7261 agacccacca ttactcactc gctatcttcg aattcacccc cagatctggg agcaccaaat
     7321 tgctctgagg cttgagattc taggatgtga ggcccagcag caatactgag gtagcctctg
     7381 catcacctgc ttattcccct tcctcagctc aaagattgtc ttaatgtttt attgctgtga
     7441 agagacacta tgaccatggc aactcttata aaataaagca tttaatcagg gcttacttat
     7501 agtttaaaaa aaaaaaaaaa aa
//
