LOCUS       NM_010168               1994 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus coagulation factor II (F2), mRNA.
ACCESSION   NM_010168
VERSION     NM_010168.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Burzynski,L.C., Morales-Maldonado,A., Rodgers,A., Kitt,L.A.,
            Humphry,M., Figg,N., Bennett,M.R. and Clarke,M.C.H.
  TITLE     Thrombin-activated interleukin-1alpha drives atherogenesis, but
            also promotes vascular smooth muscle cell proliferation and
            collagen production
  JOURNAL   Cardiovasc Res 119 (12), 2179-2189 (2023)
   PUBMED   37309666
  REMARK    GeneRIF: Thrombin-activated interleukin-1alpha drives
            atherogenesis, but also promotes vascular smooth muscle cell
            proliferation and collagen production.
            Erratum:[Cardiovasc Res. 2024 Feb 27;120(1):121. doi:
            10.1093/cvr/cvad128.. PMID: 37607022]
REFERENCE   3  (bases 1 to 1994)
  AUTHORS   Sheu,M.L., Pan,L.Y., Yang,C.N., Sheehan,J., Pan,L.Y., You,W.C.,
            Wang,C.C. and Pan,H.C.
  TITLE     Thrombin-Induced Microglia Activation Modulated through Aryl
            Hydrocarbon Receptors
  JOURNAL   Int J Mol Sci 24 (14), 11416 (2023)
   PUBMED   37511175
  REMARK    GeneRIF: Thrombin-Induced Microglia Activation Modulated through
            Aryl Hydrocarbon Receptors.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1994)
  AUTHORS   Parunov,L.A., Liang,Y., Lu,Q., Shibeko,A.M., Tucker,E.I., Lee,T.K.,
            Ataullakhanov,F.I., Scott,D.E. and Ovanesov,M.V.
  TITLE     Thrombogenic potential of picomolar coagulation factor XIa is
            mediated by thrombin wave propagation
  JOURNAL   Blood Adv 7 (11), 2622-2631 (2023)
   PUBMED   36724509
  REMARK    GeneRIF: Thrombogenic potential of picomolar coagulation factor XIa
            is mediated by thrombin wave propagation.
REFERENCE   5  (bases 1 to 1994)
  AUTHORS   Shavit-Stein,E., Mindel,E., Gofrit,S.G., Chapman,J. and Maggio,N.
  TITLE     Ischemic stroke in PAR1 KO mice: Decreased brain plasmin and
            thrombin activity along with decreased infarct volume
  JOURNAL   PLoS One 16 (3), e0248431 (2021)
   PUBMED   33720950
  REMARK    GeneRIF: Ischemic stroke in PAR1 KO mice: Decreased brain plasmin
            and thrombin activity along with decreased infarct volume.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1994)
  AUTHORS   Krawczak,M., Wacey,A. and Cooper,D.N.
  TITLE     Molecular reconstruction and homology modelling of the catalytic
            domain of the common ancestor of the haemostatic
            vitamin-K-dependent serine proteinases
  JOURNAL   Hum Genet 98 (3), 351-370 (1996)
   PUBMED   8707309
REFERENCE   7  (bases 1 to 1994)
  AUTHORS   Sun,J., Rose,J.B. and Bird,P.
  TITLE     Gene structure, chromosomal localization, and expression of the
            murine homologue of human proteinase inhibitor 6 (PI-6) suggests
            divergence of PI-6 from the ovalbumin serpins
  JOURNAL   J Biol Chem 270 (27), 16089-16096 (1995)
   PUBMED   7608171
REFERENCE   8  (bases 1 to 1994)
  AUTHORS   Soifer,S.J., Peters,K.G., O'Keefe,J. and Coughlin,S.R.
  TITLE     Disparate temporal expression of the prothrombin and thrombin
            receptor genes during mouse development
  JOURNAL   Am J Pathol 144 (1), 60-69 (1994)
   PUBMED   8291612
REFERENCE   9  (bases 1 to 1994)
  AUTHORS   Banfield,D.K. and MacGillivray,R.T.
  TITLE     Partial characterization of vertebrate prothrombin cDNAs:
            amplification and sequence analysis of the B chain of thrombin from
            nine different species
  JOURNAL   Proc Natl Acad Sci U S A 89 (7), 2779-2783 (1992)
   PUBMED   1557383
REFERENCE   10 (bases 1 to 1994)
  AUTHORS   Degen,S.J., Schaefer,L.A., Jamison,C.S., Grant,S.G.,
            Fitzgibbon,J.J., Pai,J.A., Chapman,V.M. and Elliott,R.W.
  TITLE     Characterization of the cDNA coding for mouse prothrombin and
            localization of the gene on mouse chromosome 2
  JOURNAL   DNA Cell Biol 9 (7), 487-498 (1990)
   PUBMED   2222810
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL691489.21.
            
            On Feb 24, 2023 this sequence version replaced NM_010168.3.
            
            Summary: This gene encodes a vitamin K-dependent glycoprotein
            coagulation factor that plays an important role in the process of
            blood coagulation and hemostasis. The encoded protein is an
            inactive zymogen that undergoes enzymatic cleavage by the
            coagulation factor Xa to form an active serine protease that
            converts soluble fibrinogen to insoluble fibrin clot. Most of the
            mice lacking the encoded protein die at an embryonic stage due to
            defects in yolk sac vasculature, while the rare nenonates succumb
            to hemorrhage on the first postnatal day. [provided by RefSeq, Apr
            2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC013662.1, X52308.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AL691489.21        176363-176463       c
            102-265             AL691489.21        175823-175986       c
            266-290             AL691489.21        175216-175240       c
            291-341             AL691489.21        174944-174994       c
            342-447             AL691489.21        173236-173341       c
            448-584             AL691489.21        173021-173157       c
            585-902             AL691489.21        170554-170871       c
            903-1016            AL691489.21        170198-170311       c
            1017-1143           AL691489.21        169995-170121       c
            1144-1311           AL691489.21        169196-169363       c
            1312-1485           AL691489.21        168705-168878       c
            1486-1667           AL691489.21        168367-168548       c
            1668-1738           AL691489.21        165747-165817       c
            1739-1994           AL691489.21        165363-165618       c
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 50.63 cM"
     gene            1..1994
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /note="coagulation factor II"
                     /db_xref="GeneID:14061"
                     /db_xref="MGI:MGI:88380"
     exon            1..101
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1879
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /EC_number="3.4.21.5"
                     /note="prothrombin; thrombin"
                     /codon_start=1
                     /product="prothrombin preproprotein"
                     /protein_id="NP_034298.1"
                     /db_xref="CCDS:CCDS16434.1"
                     /db_xref="GeneID:14061"
                     /db_xref="MGI:MGI:88380"
                     /translation="MSHVRGLGLPGCLALAALVSLVHSQHVFLAPQQALSLLQRVRRA
                     NSGFLEELRKGNLERECVEEQCSYEEAFEALESPQDTDVFWAKYTVCDSVRKPRETFM
                     DCLEGRCAMDLGVNYLGTVNVTHTGIQCQLWRSRYPHKPEINSTTHPGADLKENFCRN
                     PDSSTTGPWCYTTDPTVRREECSVPVCGQEGRTTVVMTPRSGGSKDNLSPPLGQCLTE
                     RGRLYQGNLAVTTLGSPCLPWNSLPAKTLSKYQDFDPEVKLVENFCRNPDWDEEGAWC
                     YVAGQPGDFEYCNLNYCEEAVGEENYDVDESIAGRTTDAEFHTFFNEKTFGLGEADCG
                     LRPLFEKKSLKDTTEKELLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASL
                     ISDRWVLTAAHCILYPPWDKNFTENDLLVRIGKHSRTRYERNVEKISMLEKIYVHPRY
                     NWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLLRAGYKGRVTGWGNLRETW
                     TTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDACEGDSG
                     GPFVMKSPFNNRWYQMGIVSWGEGCDRKGKYGFYTHVFRLKRWIQKVIDQFG"
     exon            102..265
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            266..290
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            291..341
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            342..447
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            448..584
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            585..902
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1016
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1143
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1311
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1485
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1667
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1738
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1994
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1973..1978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /note="hexamer: AATAAA"
     polyA_site      1994
                     /gene="F2"
                     /gene_synonym="Cf-2; Cf2; FII"
                     /note="major polyA site"
ORIGIN      
        1 agatcccagg agctgacaca ctatgtcgca cgtccgcggc ctgggcctcc ctggctgctt
       61 ggctctggct gccctggtta gcctggtaca cagccagcat gtgttcctgg cgcctcagca
      121 agcactatcg ctgctccagc gggtccgcag agccaacagt ggcttcctgg aggagctccg
      181 aaagggcaac ctagagcgtg agtgcgtgga agagcagtgc agctatgagg aggcctttga
      241 ggccctggag tctccccaag acacagacgt gttctgggcc aagtacacag tgtgtgattc
      301 agtgaggaaa cctcgagaga ctttcatgga ctgtctggaa ggtcgctgtg ctatggatct
      361 gggtgtgaac taccttggga ctgtgaatgt cacccatacc ggtatccagt gccagctgtg
      421 gcggagccgc tacccccaca agcctgaaat caactccacc acccatcctg gggcagacct
      481 gaaggagaac ttctgccgca atccagacag cagtaccacg ggaccctggt gctacaccac
      541 agatcctact gtgcgcagag aggaatgcag tgtccctgtc tgtggccagg agggccgtac
      601 cactgtggtg atgactcctc gctctggagg ttccaaggac aatctgtcac ctccactggg
      661 acagtgcctc actgagcgtg gccgactata ccaggggaat ttggctgtga caacacttgg
      721 gtccccctgc ctgccctgga acagcttacc agccaagacc ctgagcaagt accaagactt
      781 cgaccccgag gtgaaactcg tggaaaactt ctgccgcaac ccagactggg atgaggaggg
      841 cgcatggtgc tatgttgcgg ggcagcctgg cgactttgaa tactgcaacc tcaactactg
      901 cgaggaggcg gtgggagagg agaactatga tgtggacgag tccatcgcgg gacgcaccac
      961 cgatgcggag ttccacacct tcttcaatga gaagaccttc ggccttgggg aggcagactg
     1021 tggcctgcgg cctttgttcg agaagaagtc gttgaaagac acaaccgaaa aggagcttct
     1081 tgactcttac atagacgggc gcatcgtgga gggctgggac gctgagaagg gtatcgcccc
     1141 ctggcaggtg atgctttttc ggaagagtcc ccaagagctg ctgtgtgggg ccagccttat
     1201 cagtgaccga tgggtcctca ctgctgccca ctgcattctg tacccaccct gggacaagaa
     1261 cttcactgag aatgacctcc tggtgcgcat tggcaagcat tcccgaacca gatatgagcg
     1321 gaatgttgaa aagatctcca tgctggaaaa gatctacgtc caccccagat ataactggcg
     1381 ggagaaccta gaccgcgata tcgctctgct caagctaaag aaacctgtac ccttcagtga
     1441 ctatattcac cccgtgtgtt tgccagacaa gcagacagta accagcttgc tccgggctgg
     1501 ttataaaggg cgggtgacag gctggggcaa ccttcgggag acatggacaa ccaacatcaa
     1561 tgagatacag cccagcgtcc tgcaggtggt gaacctgccc attgtagagc ggccagtgtg
     1621 caaggcctcc acccggattc gaattactga caacatgttc tgtgctggct tcaaggtgaa
     1681 tgacaccaag cgaggagatg cttgtgaagg tgacagtgga ggaccttttg tcatgaagag
     1741 cccctttaac aaccgctggt atcaaatggg tattgtctca tggggtgaag gatgtgaccg
     1801 gaaggggaaa tacggcttct acacgcatgt gttccgtctg aaaaggtgga tacagaaagt
     1861 cattgatcaa tttggatagg tgtgtgggtg ggtaaaggac ctacactatg gaatcctcat
     1921 tgcaaaatca cagaaaccaa tcaaattact tttgtggttt gtttctgttc tcaataaaag
     1981 tgattgtcag taga
//
