LOCUS       NM_001033211            1840 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus EZH inhibitory protein (Ezhip), transcript variant 2,
            mRNA.
ACCESSION   NM_001033211 XM_159101 XM_906707
VERSION     NM_001033211.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ragazzini,R., Perez-Palacios,R., Baymaz,I.H., Diop,S., Ancelin,K.,
            Zielinski,D., Michaud,A., Givelet,M., Borsos,M., Aflaki,S.,
            Legoix,P., Jansen,P.W.T.C., Servant,N., Torres-Padilla,M.E.,
            Bourc'his,D., Fouchet,P., Vermeulen,M. and Margueron,R.
  TITLE     EZHIP constrains Polycomb Repressive Complex 2 activity in germ
            cells
  JOURNAL   Nat Commun 10 (1), 3858 (2019)
   PUBMED   31451685
  REMARK    GeneRIF: Deletion of Ezhip (EZH inhibitory protein) in mice leads
            to a global increase in H3K27me2/3 deposition both during
            spermatogenesis and at late stages of oocyte maturation. This does
            not affect the initial number of follicles but is associated with a
            reduction of follicles in aging. Study uncovers Ezhip as a
            regulator of chromatin landscape in gametes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Jain,S.U., Do,T.J., Lund,P.J., Rashoff,A.Q., Diehl,K.L.,
            Cieslik,M., Bajic,A., Juretic,N., Deshmukh,S., Venneti,S.,
            Muir,T.W., Garcia,B.A., Jabado,N. and Lewis,P.W.
  TITLE     PFA ependymoma-associated protein EZHIP inhibits PRC2 activity
            through a H3 K27M-like mechanism
  JOURNAL   Nat Commun 10 (1), 2146 (2019)
   PUBMED   31086175
  REMARK    GeneRIF: Study revealed that CXORF67 contains a highly conserved
            'K27M-like' sequence that is necessary and sufficient to inhibit
            PRC2 activity and reduce cellular H3K27me3 levels. Biochemical and
            cell-based studies demonstrate that CXORF67 functions as a
            K27M-like peptidyl inhibitor of PRC2. Therefore, study propose the
            name EZHIP (Enhancer of Zeste Homologs Inhibitory Protein) as a
            more descriptive for the function of CXORF67.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1840)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX649475.8.
            
            On Oct 23, 2009 this sequence version replaced NM_001033211.2.
            
            Transcript Variant: This variant (2) lacks an internal segment in
            the coding region, compared to variant 1. The resulting protein
            (isoform 2) is shorter when it is compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166653.1, AK136005.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-282               BX649475.8         6875-7156           c
            283-1840            BX649475.8         4954-6511           c
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 2.7 cM"
     gene            1..1840
                     /gene="Ezhip"
                     /gene_synonym="KIP75"
                     /note="EZH inhibitory protein"
                     /db_xref="GeneID:102991"
                     /db_xref="MGI:MGI:2147968"
     exon            1..282
                     /gene="Ezhip"
                     /gene_synonym="KIP75"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1505
                     /gene="Ezhip"
                     /gene_synonym="KIP75"
                     /note="isoform 2 is encoded by transcript variant 2;
                     K27M-like inhibitor of PRC2"
                     /codon_start=1
                     /product="EZH inhibitory protein isoform 2"
                     /protein_id="NP_001028383.2"
                     /db_xref="CCDS:CCDS52982.1"
                     /db_xref="GeneID:102991"
                     /db_xref="MGI:MGI:2147968"
                     /translation="MASSSSPERGLEALRDTDESEGEAPGPSGPRGRGGPSGAGSALR
                     LRSLEAEMAAACVTSTAVGNLEAPGPTLRSSTSQGSGSTPVPEALRCAESSRAESDQS
                     SPAGRELRQQASPRAPDDDDDGDGGPDPRGSGTPEGWVLRSGVVPFGRRSSASEVSPE
                     EVRPEAQCTGWNLRPRPRSSASAVSPEARPKAQSAGRNLRPRPRSSASVVSPEARPKA
                     QSAGRNLRPRPRSSASVVSPEARPEAQSAGRNLRPRATPRVPVAPSSTTRSSSDRGSS
                     RAPRSRSRSRSCSTPRLGSDHQRSRKIKMRLDLQVDREPESEAEQEEQELESEPGPSS
                     RPQASRSSSRFAVPGRSSLAAEDSPPRRPVRMRASSPSPPGRLYPLPKHYFEGVHSPS
                     SSSSESSSVSSSHSPLNKAPDPGSSPPLSSLSGPNPFWLALIADLDNLDSSSPRVPGE
                     EIEAAPHTREEEDKKCRG"
     exon            283..1840
                     /gene="Ezhip"
                     /gene_synonym="KIP75"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1811..1816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ezhip"
                     /gene_synonym="KIP75"
                     /note="hexamer: AATAAA"
     polyA_site      1835
                     /gene="Ezhip"
                     /gene_synonym="KIP75"
                     /note="major polyA site"
ORIGIN      
        1 tgtatgtggt cactacccag catcctcgct ggctctcagg aagccatttg agcgtgcaaa
       61 gccgagcgaa gcatttctcc agacgctgtt ggaccgacat ggccagctct tcgtctccag
      121 agagggggct ggaggcgctc agagacactg acgagtccga aggtgaagct ccggggcctt
      181 cggggcccag aggccgaggt ggtccctcgg gggcaggcag cgcgctgcgt ctccggagcc
      241 tcgaagccga aatggcggcg gcctgcgtca cctccacggc cgtggggaac ctagaagccc
      301 cggggcctac cctcaggagc tccacctcac agggctcggg gtctactcca gtccccgagg
      361 ccctccgctg tgccgagtcc agccgtgccg agagtgacca gtccagccct gctggccgag
      421 aactgcgtca acaggccagt ccacgggctc ccgatgatga tgatgacggt gatggtggtc
      481 ctgatcccag aggcagcggg acccccgaag gctgggtcct ccgttccgga gttgtacctt
      541 tcggtcgtag gagcagtgca tcggaggtca gccccgaaga ggttcgcccc gaagcccagt
      601 gcacaggctg gaatttacgt cctcgtcctc ggagcagtgc atcagcggtc agccctgaag
      661 ctcgccccaa agcccagagc gcaggcagga atttacgtcc tcgtcctcgg agcagtgcat
      721 cagtggtcag ccctgaagct cgccccaaag cccagagcgc aggcaggaat ttacgtcctc
      781 gtcctcggag cagtgcatca gtggtcagcc ccgaagctcg cccggaagcc cagagcgcag
      841 gcaggaattt acgtcctcgg gccactccac gagttcctgt tgctcccagc agtaccacga
      901 ggtccagttc tgatagaggc agcagccgtg ccccacggag ccggagccga agccgcagct
      961 gcagtacacc caggctcggc tctgaccatc aacggagccg taaaataaag atgaggctgg
     1021 atctgcaagt ggaccgtgag ccggaatcgg aggcagagca agaagagcaa gagctggagt
     1081 cagaacctgg ccctagcagc cggccgcaag cgagccgttc ctcctctagg tttgcagtcc
     1141 cagggagatc ctctctagca gccgaagact ctcctccaag gcgcccagtt cgtatgcgag
     1201 cctcttcccc ttctcctcct ggtaggctct atcctttgcc caagcactat tttgaaggtg
     1261 tccattctcc ctcctcatct tccagtgagt cctccagtgt ttcaagttcc cattctcccc
     1321 tcaataaggc accagatcca ggctcctcac ctccattaag ctctctctct ggtcctaatc
     1381 ccttttggct tgccttgata gctgacctgg ataaccttga ttcttcctct cccagagtgc
     1441 caggggaaga aatagaggca gcccctcaca ctcgtgaaga ggaagataag aagtgtaggg
     1501 gatgaccctt actgccatac tctgcccttg ctgtaattca ttgtgagggt tctcccaggt
     1561 gcagaaaagg aattggcacg tatcccctgt tggttgtgtg attatctctg aggtttctag
     1621 ggttctacct actcaccagt agtctgggaa gtttgaagtt acctgttccc ctcaccccaa
     1681 ctatctagca tttataagga ctttggttcc attcagtacc cccttttatc tttaaaacag
     1741 cctgtattgt ttgctgttgg gtttgggtgg ggtttttatt ccaagttgct cttcagtaat
     1801 taagtatttt aataaagtaa gttttatttt aaaaatttag
//
