LOCUS       NM_001403760            5286 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Mus musculus EYA transcriptional coactivator and phosphatase 3
            (Eya3), transcript variant 9, mRNA.
ACCESSION   NM_001403760 XM_006538537
VERSION     NM_001403760.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Wang,Y., Pandey,R.N., York,A.J., Mallela,J., Nichols,W.C., Hu,Y.C.,
            Molkentin,J.D., Wikenheiser-Brokamp,K.A. and Hegde,R.S.
  TITLE     The EYA3 tyrosine phosphatase activity promotes pulmonary vascular
            remodeling in pulmonary arterial hypertension
  JOURNAL   Nat Commun 10 (1), 4143 (2019)
   PUBMED   31515519
  REMARK    GeneRIF: these observations establish EYA3 as a disease-modifying
            target whose function in the pathophysiology of pulmonary arterial
            hypertension.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5286)
  AUTHORS   Sano,T. and Nagata,S.
  TITLE     Characterization of the threonine-phosphatase of mouse eyes absent
            3
  JOURNAL   FEBS Lett 585 (17), 2714-2719 (2011)
   PUBMED   21821028
  REMARK    GeneRIF: Data showed that the 68-amino acid domain between
            positions 53 and 120 was necessary and sufficient for its
            threonine-phosphatase activity.
REFERENCE   4  (bases 1 to 5286)
  AUTHORS   Masumoto,K.H., Ukai-Tadenuma,M., Kasukawa,T., Nagano,M., Uno,K.D.,
            Tsujino,K., Horikawa,K., Shigeyoshi,Y. and Ueda,H.R.
  TITLE     Acute induction of Eya3 by late-night light stimulation triggers
            TSHbeta expression in photoperiodism
  JOURNAL   Curr Biol 20 (24), 2199-2206 (2010)
   PUBMED   21129973
  REMARK    GeneRIF: Data demonstrate that Eya3 and its partner Six1
            synergistically activate TSHbeta expression and that this
            activation is further enhanced by Tef and Hlf.
REFERENCE   5  (bases 1 to 5286)
  AUTHORS   Kalousova,A., Mavropoulos,A., Adams,B.A., Nekrep,N., Li,Z.,
            Krauss,S., Stainier,D.Y. and German,M.S.
  TITLE     Dachshund homologues play a conserved role in islet cell
            development
  JOURNAL   Dev Biol 348 (2), 143-152 (2010)
   PUBMED   20869363
REFERENCE   6  (bases 1 to 5286)
  AUTHORS   Bulfone,A., Gattuso,C., Marchitiello,A., Pardini,C., Boncinelli,E.,
            Borsani,G., Banfi,S. and Ballabio,A.
  TITLE     The embryonic expression pattern of 40 murine cDNAs homologous to
            Drosophila mutant genes (Dres): a comparative and topographic
            approach to predict gene function
  JOURNAL   Hum Mol Genet 7 (13), 1997-2006 (1998)
   PUBMED   9817915
REFERENCE   7  (bases 1 to 5286)
  AUTHORS   Cox,K.B., Johnson,K.R. and Wood,P.A.
  TITLE     Chromosomal locations of the mouse fatty acid oxidation genes
            Cpt1a, Cpt1b, Cpt2, Acadvl, and metabolically related Crat gene
  JOURNAL   Mamm Genome 9 (8), 608-610 (1998)
   PUBMED   9680378
REFERENCE   8  (bases 1 to 5286)
  AUTHORS   Duncan,M.K., Kos,L., Jenkins,N.A., Gilbert,D.J., Copeland,N.G. and
            Tomarev,S.I.
  TITLE     Eyes absent: a gene family found in several metazoan phyla
  JOURNAL   Mamm Genome 8 (7), 479-485 (1997)
   PUBMED   9195991
  REMARK    Erratum:[Mamm Genome 1997;8(11):877]
REFERENCE   9  (bases 1 to 5286)
  AUTHORS   Zimmerman,J.E., Bui,Q.T., Steingrimsson,E., Nagle,D.L., Fu,W.,
            Genin,A., Spinner,N.B., Copeland,N.G., Jenkins,N.A., Bucan,M. and
            Bonini,N.M.
  TITLE     Cloning and characterization of two vertebrate homologs of the
            Drosophila eyes absent gene
  JOURNAL   Genome Res 7 (2), 128-141 (1997)
   PUBMED   9049631
REFERENCE   10 (bases 1 to 5286)
  AUTHORS   Xu,P.X., Woo,I., Her,H., Beier,D.R. and Maas,R.L.
  TITLE     Mouse Eya homologues of the Drosophila eyes absent gene require
            Pax6 for expression in lens and nasal placode
  JOURNAL   Development 124 (1), 219-231 (1997)
   PUBMED   9006082
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL627130.13.
            
            On Apr 4, 2022 this sequence version replaced XM_006538537.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.5218.1,
                                           SRR10223970.6849.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               AL627130.13        40164-40263
            101-196             AL627130.13        57801-57896
            197-240             AL627130.13        63003-63046
            241-320             AL627130.13        68263-68342
            321-387             AL627130.13        71297-71363
            388-524             AL627130.13        74004-74140
            525-662             AL627130.13        81864-82001
            663-748             AL627130.13        90813-90898
            749-932             AL627130.13        94070-94253
            933-1069            AL627130.13        95855-95991
            1070-1153           AL627130.13        100138-100221
            1154-1243           AL627130.13        102129-102218
            1244-1302           AL627130.13        105460-105518
            1303-1463           AL627130.13        108071-108231
            1464-1578           AL627130.13        111053-111167
            1579-1700           AL627130.13        112950-113071
            1701-1801           AL627130.13        120320-120420
            1802-5286           AL627130.13        122387-125871
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 65.68 cM"
     gene            1..5286
                     /gene="Eya3"
                     /note="EYA transcriptional coactivator and phosphatase 3"
                     /db_xref="GeneID:14050"
                     /db_xref="MGI:MGI:109339"
     exon            1..100
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            101..196
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     CDS             164..1882
                     /gene="Eya3"
                     /EC_number="3.1.3.48"
                     /note="isoform 8 is encoded by transcript variant 9; eyes
                     absent homolog 3; eyes absent 3 homolog; protein
                     phosphatase EYA3"
                     /codon_start=1
                     /product="protein phosphatase EYA3 isoform 8"
                     /protein_id="NP_001390689.1"
                     /db_xref="GeneID:14050"
                     /db_xref="MGI:MGI:109339"
                     /translation="MEEEQDLPEQPVKKAKMQEPREQTLSQVNNPDASDEKPETSSLA
                     SNLSMSEEIMTCTDYIPRSSNDYTSQMYSAKPYAHILSVPVSETTYPGQTQYQTLQQS
                     QPYAVYPQATQTYGLPPFGALWPGMKPESGLIQTPSPSQHSVLTCTTGLTTSQPSSAH
                     YSYPIQASSTNASLIPTSSAIANIPAAAVASISNQDYPTYTILGQNQYQACYPSSSFG
                     VTGQTNSDAETTTLAATTYQTEKPSAMVPAPATQRLPSDSSASPPLSQTTPNKDADDQ
                     ARKNMTVKNRGKRKADASSSQDSELERVFLWDLDETIIIFHSLLTGSYAQKYGKDPTV
                     VIGSGLTMEEMIFEVADTHLFFNDLEECDQVHVEDVASDDNGQDLSNYSFSTDGFSGS
                     GGSGSHGSSVGVQGGVDWMRKLAFRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAE
                     IEVLTDSWLGTALKSLLLIQSRKNCANVLITTTQLVPALAKVLLYGLGEIFPIENIYS
                     ATKIGKESCFERIVSRFGKKVTYVVIGDGRDEEIAAKQHNMPFWRITNHGDLVSLHQA
                     LELDFL"
     exon            197..240
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            241..320
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            321..387
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            388..524
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            525..662
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            663..748
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            749..932
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1069
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1153
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1243
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1302
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1463
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1578
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1700
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1801
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..5286
                     /gene="Eya3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2634..2639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eya3"
                     /note="hexamer: AATAAA"
     polyA_site      2658
                     /gene="Eya3"
                     /note="major polyA site"
     regulatory      5267..5272
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eya3"
                     /note="hexamer: AATAAA"
     polyA_site      5286
                     /gene="Eya3"
                     /note="major polyA site"
ORIGIN      
        1 atccgggtca cgctaacgcc gcggtttccg ccactctgtt ggttccagag tgggtccgta
       61 atcgtcccat gtcgtgctgg gcagtgtctg cagccgtctg tttgtgaaga ccgcagagac
      121 ctgcaattca gtcccctaag tgtgttagga gatagtcgtc ctcatggaag aagagcaaga
      181 cctaccagag caaccggtga aaaaagccaa gatgcaggaa ccaagagaac agactttaag
      241 tcaagtaaac aacccagatg ccagtgatga gaagcctgag acatccagcc ttgcgtcaaa
      301 tctcagcatg tcagaggaaa ttatgacatg caccgattac atccctcgct catccaatga
      361 ttatacctca caaatgtatt ctgcaaaacc ttatgcacac atcctctcag ttcctgtttc
      421 ggaaaccact tatcctgggc agactcagta ccagacactg cagcaatctc aaccctacgc
      481 tgtctaccct caggcaaccc aaacttacgg actacctcct ttcggtgcac tgtggccagg
      541 tatgaaacct gaaagtggtt taattcagac tccatctcca agtcaacaca gtgttcttac
      601 ctgcactaca gggttaacca caagccagcc aagctcagca cattattctt atcccattca
      661 agcttcaagc acaaatgcca gcctgatacc cacttcatct gcaattgcca atattccagc
      721 agcagctgtg gccagcatct caaaccagga ttatcccacc tatactattc ttggacagaa
      781 tcagtaccag gcctgctacc ccagttccag ctttggagtc acaggtcaga ctaacagtga
      841 tgctgagacc acaacattag cagctacaac ataccagacg gagaagccta gtgctatggt
      901 gcctgcacca gccacacaga ggcttccctc cgactcctct gcaagcccac ctttgtccca
      961 gactacacca aataaagatg ctgatgatca ggccaggaaa aacatgactg tcaagaaccg
     1021 gggcaagagg aaagctgatg ccagctcttc ccaggacagt gaattggaac gggtatttct
     1081 ctgggacttg gacgaaacca tcatcatctt tcattccctt ctcactggat cctatgctca
     1141 gaagtatgga aaggacccaa cagtagtaat tggctcaggt ttaaccatgg aagaaatgat
     1201 ttttgaagtg gctgatacac atctattttt caatgactta gaggagtgtg accaggtgca
     1261 tgtggaagat gtggcttctg atgacaatgg ccaggatttg agcaactaca gtttctccac
     1321 agatggtttc agtggttcag gaggcagtgg tagccacggc tcatctgtgg gcgttcaggg
     1381 aggtgtggac tggatgagga aactggcctt tcgctaccga aaagtgaggg aaatctacga
     1441 caagcataaa agcaatgtgg gtggcctcct cagcccccag aggaaggaag cactgcagag
     1501 actcagagca gagatcgagg tgctgacgga ctcctggtta ggaactgcgc tcaagtcctt
     1561 gcttctcatc cagtctcgaa agaactgtgc gaatgttctg atcactacca cgcagttggt
     1621 tccagccctg gccaaggttc tcctgtatgg actaggagag atatttccta ttgaaaacat
     1681 ctacagtgct accaaaatcg gtaaggagag ctgctttgag agaattgttt cgaggtttgg
     1741 gaaaaaagtc acatatgtag tgattggaga tggacgagat gaagaaattg cagccaagca
     1801 gcacaacatg cccttctgga ggatcacaaa ccacggagat ctggtgtccc tgcaccaggc
     1861 tttagagctt gacttcctct gagaactgga atgtggaccc ttccttcctt gtgagcttct
     1921 ctttacctcc aacaggagcc agaagccaga accctctgag ccccttctct cctgtctgtc
     1981 tctcgggtct cagtgccccc tcccccttct cttctgctct ttctccctcc atgaaatgct
     2041 ggcgagaaca caatcagaac caacaactgc agttattctg agtgagctgc agcccatgtc
     2101 ccctgtacag caagaggtgg ctggatagag ctgcagaccg gctgccgcta ccgtgcttta
     2161 atttttctgt ttcaattgaa aaaggaagaa caagaaaagc cgatgcttgg ggcacagttg
     2221 tactcttgct gcatgacgga ctgaccggga gctgctcctg atgtggatgg aaactgtccc
     2281 cttgagatcg tcttctggtc tcttagctat agaatgtctc tcccagtgaa tgggtatgtt
     2341 atttttatag gtgagggtct ggtctctaca gcagcctccc ccacttttct atgaagaaag
     2401 ccgtgtgtaa agtttccgtg acagtagtaa tggaaatatc taaaatacct ccgtgactgg
     2461 acaggacaga ggctccagtg ctctgctccc tggggaggct ccttgccatc tacaaggccc
     2521 tggtcactta ctttagactg tggcatctcc catcttgtaa gcctcggtct ccttggcagt
     2581 cctccttcat ctgtaactaa atggcacaac cctggaaact ccttcccata gacaataaat
     2641 ctaagccttt agcttgtact tcagaagttc tgtcccagga ggctcagcct ccagactgga
     2701 gagaagggct cagatttctt aggactttca cctcgtgctc ctgcagagca gtacctgttc
     2761 ccagcagatg cttcctctgt ggtcggctca gtcgtcagat gctggcctca cgtcctgtcc
     2821 tcacagctac ctgtctctgt gcagctctgg ccagccccta cccactcatt gcaagtcaga
     2881 aaggccaagg gggcaggctc tagctgcctc cttcacctgc acttacatgc ggtgatccca
     2941 ccttgtattt atacagatct ctgcctacga gtggagagca gagagctaga gtcaaaaccc
     3001 atgaaatatc ccactgctta gagtccatgc tgaagtggga aaccaggcca gaaagacttt
     3061 cttgacttaa gtgcttaaaa atacaatcct gagctaggtg caagagcacc tctcaaggct
     3121 gcccctagtc agtcaggagg gaaactgggc cttagtgcca gcccgcaagc ccagctgtgt
     3181 tttggtttta ttttggtttt tgtttgggtt ttttgtttgt tttttacggc actatccctt
     3241 caggaccaat gtcggtgcct gtttaattgg gttaaacgtc cctagagttg aggaaatgtg
     3301 cagccttggt cttcaggagg tccttgacca acccctagct atagtcactg tccgcagcga
     3361 taccacagcc cagaggcaaa attcctcttc atgctaggct accagataac gacagaaagg
     3421 ctgacaccac cttcagaact gttggacact tctgagtact tctgagacca gcagttgagg
     3481 aaggggctca gacaggaggt gaggtttgtg tcttggccat tcatttggct ttgttgtgag
     3541 gtactgggtg ggagcaagga gctagagtct aagtcagacc ctcctaggct gccattagca
     3601 ccacaccgct accctagtct ggccagcctt gcttggtggt ccaacctggg aaactccagg
     3661 atgagtttta cttccaagtc tcagtccttc cctcattaac agtggagttt cttgtgctgg
     3721 acaacgccac ctattagtgc tgtgcttcag gctcaccaga ctgccaagtg gcgacacacc
     3781 cttctgcaca caggtaccac cacaaacact tcaggggaga ctcttcttag aatataacag
     3841 gtaatccctt tcctcttgcc ctgactggag aggcaaggag gtgttctgag ctgagtggct
     3901 actgttccca aagcagctac cctgtcaggg tagacagagg agtgcattgg tctctccagg
     3961 gactgctgcc tttgtgctga ctccctccct ccctccctcc ctccctccct cccacccacc
     4021 cacagaggct tgggaaggaa aatgagcagg cagcatcaag agctgctagt aagagaaaga
     4081 cagcttggtg gtaatagcac ccgcagcttg tgcagaggtc caaggtttga gcacccacaa
     4141 ggctattcac aacctctagc tgcagctcca gggggttcgt tgccctcttc tggcctcttc
     4201 aggcaccaag cacttatata cgtgtaagaa acactcataa aatagaaatt ccttttaata
     4261 ttaaaagctg ttggaagtta gaaggcagtc cttactgaag gaacaccttg aagaaattag
     4321 ctggtgaatc ttttccctgc tggatcctgt gttctggaag attagtcagg acatctcatg
     4381 gtgacagcta gttctaggag tgtgtggggt ggcaggaatg tcctgggata caaggtggct
     4441 tgactttcga atgtaaaagt gactgtgcct tagcatcacc ctgtccagtg cccctggggc
     4501 agccggtgat gtccgcgaga gtggatacct gctccacatt ttaggtgcct acttgggctt
     4561 ttgatttggg ggtgttctct tctctgctag tcgggtgaca gctagtgtcc caagagactt
     4621 ggcacagctt gtccgtgttc acccaagctc aaccttcaca gctccaagta gaatctgact
     4681 ggcaagctgc agcagcaagc tcatggcgct tccatagaca ctgctttgaa tgggagtcac
     4741 acaactgaca ggagcaccag gcagagctcg gtgctgtcct ggaagagtgc aggctagccg
     4801 ccaagtgcca agcttgggcc tcattcctca ctctccccag gtagctgagg cccttgagta
     4861 agcgtgtgct cccggctgct cactggtcaa gcagctagaa ctacccagac acacccattg
     4921 tagatgccag gttggcgttc tgagcttccc aaacatctaa accacgaggg ggagaacccc
     4981 gcttgtgtcc cagcctcatc tgcgggccgc ttgccctccc tctaccctga gtcaattcta
     5041 gtgagcatgg ctccctggct gcctcctcac actgagcgga gccatcccag ttcaggacca
     5101 tttctacttg gatctccact ctccctctgg actccagatg cctctgtagc aggaaaaggc
     5161 atccatgggt gggatatata tatttatgtg actgcgggca tttgtggcta tagagtcctt
     5221 gaccataatg ttatatgtaa tgggaactat cttataaact tgaaaaaata aagtttttat
     5281 tttcta
//
