LOCUS       NM_001163514            2819 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus exostosin-like glycosyltransferase 2 (Extl2),
            transcript variant 2, mRNA.
ACCESSION   NM_001163514
VERSION     NM_001163514.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Pu,A., Mishra,M.K., Dong,Y., Ghorbanigazar,S., Stephenson,E.L.,
            Rawji,K.S., Silva,C., Kitagawa,H., Sawcer,S. and Yong,V.W.
  TITLE     The glycosyltransferase EXTL2 promotes proteoglycan deposition and
            injurious neuroinflammation following demyelination
  JOURNAL   J Neuroinflammation 17 (1), 220 (2020)
   PUBMED   32703234
  REMARK    GeneRIF: The glycosyltransferase EXTL2 promotes proteoglycan
            deposition and injurious neuroinflammation following demyelination.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Nadanaka,S., Hashiguchi,T. and Kitagawa,H.
  TITLE     Aberrant glycosaminoglycan biosynthesis by tumor suppressor EXTL2
            deficiency promotes liver inflammation and tumorigenesis through
            Toll-like 4 receptor signaling
  JOURNAL   FASEB J 34 (6), 8385-8401 (2020)
   PUBMED   32347583
  REMARK    GeneRIF: Aberrant glycosaminoglycan biosynthesis by tumor
            suppressor EXTL2 deficiency promotes liver inflammation and
            tumorigenesis through Toll-like 4 receptor signaling.
REFERENCE   3  (bases 1 to 2819)
  AUTHORS   Uijtdewilligen,P.J.E., Versteeg,E.M., van de Westerlo,E.M.A., van
            der Vlag,J., Daamen,W.F. and van Kuppevelt,T.H.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2819)
  AUTHORS   Nadanaka,S. and Kitagawa,H.
  TITLE     Exostosin-like 2 regulates FGF2 signaling by controlling the
            endocytosis of FGF2
  JOURNAL   Biochim Biophys Acta Gen Subj 1862 (4), 791-799 (2018)
   PUBMED   29305908
  REMARK    GeneRIF: Data show that exostosin-like 2 (EXTL2) controls
            fibroblast growth factor 2 (FGF2) signaling through regulation of
            heparan sulfate biosynthesis.
REFERENCE   5  (bases 1 to 2819)
  AUTHORS   Nadanaka,S. and Kitagawa,H.
  TITLE     EXTL2 controls liver regeneration and aortic calcification through
            xylose kinase-dependent regulation of glycosaminoglycan
            biosynthesis
  JOURNAL   Matrix Biol 35, 18-24 (2014)
   PUBMED   24176719
  REMARK    GeneRIF: EXTL2 controls liver regeneration and aortic calcification
            through xylose kinase-dependent regulation of glycosaminoglycan
            biosynthesis.[review]
            Review article
REFERENCE   6  (bases 1 to 2819)
  AUTHORS   Nadanaka,S., Kagiyama,S. and Kitagawa,H.
  TITLE     Roles of EXTL2, a member of the EXT family of tumour suppressors,
            in liver injury and regeneration processes
  JOURNAL   Biochem J 454 (1), 133-145 (2013)
   PUBMED   23734945
  REMARK    GeneRIF: EXTL2-mediated regulation of glycosaminoglycans synthesis
            is important for tissue regeneration processes following liver
            injury.
REFERENCE   7  (bases 1 to 2819)
  AUTHORS   Nadanaka,S., Zhou,S., Kagiyama,S., Shoji,N., Sugahara,K.,
            Sugihara,K., Asano,M. and Kitagawa,H.
  TITLE     EXTL2, a member of the EXT family of tumor suppressors, controls
            glycosaminoglycan biosynthesis in a xylose kinase-dependent manner
  JOURNAL   J Biol Chem 288 (13), 9321-9333 (2013)
   PUBMED   23395820
  REMARK    GeneRIF: EXTL2 functions to suppress GAG biosynthesis that is
            enhanced by a xylose kinase and the EXTL2-dependent mechanism that
            regulates GAG biosynthesis might be a 'quality control system' for
            proteoglycans
REFERENCE   8  (bases 1 to 2819)
  AUTHORS   Pedersen,L.C., Dong,J., Taniguchi,F., Kitagawa,H., Krahn,J.M.,
            Pedersen,L.G., Sugahara,K. and Negishi,M.
  TITLE     Crystal structure of an alpha 1,4-N-acetylhexosaminyltransferase
            (EXTL2), a member of the exostosin gene family involved in heparan
            sulfate biosynthesis
  JOURNAL   J Biol Chem 278 (16), 14420-14428 (2003)
   PUBMED   12562774
  REMARK    GeneRIF: crystal structure of EXTL2
REFERENCE   9  (bases 1 to 2819)
  AUTHORS   Wuyts,W. and Van Hul,W.
  TITLE     Characterization and genomic localization of the mouse Extl2 gene
  JOURNAL   Cytogenet Cell Genet 89 (3-4), 185-188 (2000)
   PUBMED   10965119
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY251302.1, AK019370.1, BC094444.1 and AK032016.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB032171.1, SRR1660811.219350.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-70                BY251302.1         1-70
            71-878              AK019370.1         2-809
            879-2692            BC094444.1         785-2598
            2693-2819           AK032016.1         2220-2346
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 50.17 cM"
     gene            1..2819
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /note="exostosin-like glycosyltransferase 2"
                     /db_xref="GeneID:58193"
                     /db_xref="MGI:MGI:1889574"
     exon            1..241
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            242..316
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             309..1301
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /EC_number="2.4.1.223"
                     /note="isoform a is encoded by transcript variant 2;
                     exostoses-like 2; exostosin-like 2; exostoses
                     (multiple)-like 2; alpha-GalNAcT EXTL2; EXT-related
                     protein 2; alpha-1,4-N-acetylhexosaminyltransferase EXTL2;
                     glucuronyl-galactosyl-proteoglycan
                     4-alpha-N-acetylglucosaminyltransferase; exotoses
                     (multiple)-like 2"
                     /codon_start=1
                     /product="exostosin-like 2 isoform a"
                     /protein_id="NP_001156986.1"
                     /db_xref="CCDS:CCDS17784.1"
                     /db_xref="GeneID:58193"
                     /db_xref="MGI:MGI:1889574"
                     /translation="MMRGCHICKLPGRVMGIRVLRFSLVVILVLLLVAGALTNLLPNI
                     KEDKMLTLRREIKSPSKSALDSFTLIMQTYNRTDLLLRLLNHYQAVPSLHKVIVVWNN
                     VGEKGPEELWNSLGPHPIPVIFKPQTANKMRNRLQVFPEVETNAVLMVDDDTLISAQD
                     LVFAFSIWQQFPDQIIGFVPRKHVSTSSGIYSYGGFELQTPGPGNGDQYSMVLIGASF
                     FNSKYLELFQKQPAAVHALIDETQNCDDIAMNFLVTRHTGKPSGIFVKPINMVNLEKE
                     TNGYSGMWHRAEHFLQRSYCINKLVNIYDGMPLKYSNIMISQFGFPYANHKSKM"
     exon            317..744
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            745..815
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            816..2819
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2120..2125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2140
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /note="major polyA site"
     regulatory      2673..2678
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2692
                     /gene="Extl2"
                     /gene_synonym="3000001D04Rik"
ORIGIN      
        1 aggaagtgga gcgtcaccca aagcgaggga ggggtggcca ctcaggaact gtcccctgct
       61 ccaggcactc actttgcagg cggcgcattt cctaggttat caattggacg ggcagagagg
      121 ttggaagccc ggtttaggaa aggggtggaa aagaggcaag caaaggagac tctcctttcg
      181 atttatgcta accaagctgc aaataaaccc gctggccccg ctgcaatctt ctgcactaga
      241 gtatcaagag agcctgttcc actgaagtac tgaagtcata aagaatgctg ctataaaaaa
      301 ttaataatat gatgaggggt tgccacatct gcaaacttcc gggaagagtc atggggattc
      361 gagtgctccg gttctccctg gtggtcatcc ttgtgttgct gctggtagcg ggggctttga
      421 ccaacttact ccccaacatc aaagaagaca agatgctcac tctgcgtagg gagatcaaat
      481 ccccgagcaa gtcggccctg gattcattca ccctcatcat gcagacatac aacagaacag
      541 acctcttact aagactctta aatcattacc aggcagtacc gagtctgcac aaggtgatcg
      601 tggtgtggaa caatgtggga gagaagggcc cagaggagtt gtggaattct ctagggcctc
      661 acccgatccc tgtcatcttc aaaccacaaa cagcaaacaa aatgagaaac cgactccagg
      721 tcttccctga agtggagacc aacgcggtgt tgatggtaga cgatgacaca ctcattagtg
      781 cccaggacct tgttttcgct ttctcaattt ggcagcaatt tcctgatcag atcataggat
      841 ttgtacctag aaagcacgtc tctacttcct ccgggatcta cagttacgga ggctttgagc
      901 tgcagacacc ggggcccggg aatggcgacc agtactccat ggtgctcatc ggagcctcct
      961 tcttcaacag caaatacctg gaactcttcc agaagcagcc tgcagccgtc cacgcgctga
     1021 tcgatgagac gcagaactgt gacgacatcg ctatgaattt cctcgtcacc aggcacactg
     1081 gcaagccttc agggatattc gtgaaaccca taaacatggt caacttggaa aaagaaacca
     1141 acgggtattc cggaatgtgg caccgggcgg agcactttct gcagagatcc tactgtataa
     1201 ataagctcgt taacatctac gacggcatgc ccttaaagta ctccaacatt atgatttccc
     1261 agtttggttt tccgtatgcc aaccataaaa gtaaaatgtg aaccggaaac aaagacaagc
     1321 ccaaaacacc gcgctgggtt ttcagtggct tctccatgct gtgtgttttt ggttttattt
     1381 ttggtttttt ttttcaagca acactatgaa cttttatcta gtccataagt ctctataata
     1441 gaaacaaaaa aaccatatgc ggtacttcta gaatataaaa cacatggacc ttcaaaagcc
     1501 aagaagttgg ccttaccggg ataggatttc ctgtgaagag cttttgtcca agatggtgac
     1561 attattgttt acatctcgag actgctcctc agtttgtttg agccctggca tttgcctcaa
     1621 ggccctacag caagctgctt ccagagactc acaagagaca attttcagct tttcggtgaa
     1681 ggtcgattgt ttttatttga agcctgaaat gcctgcggta tagggaaaag ccatgtgagg
     1741 agagtttagt ctgaactcag tgttgagagc caggaaaatt cctggttgcc ggtgtctttc
     1801 tggtccatct cacagaactg actgagccca gcacggttct aacgaagctg tcagtgctgg
     1861 tgtctctgct gttacgcctg ttggtgactg ggagtgtaag ttttcatggc tgactgttgt
     1921 ttaattggtt tttattggtt tggtttggtt tggtttttgt gtgagcctac tctgcatgga
     1981 tcactgaaat gtgccagcca tgtttcttat atgtaacttg tatgcaacaa ggaatcagtg
     2041 tgtccctgta atctgtcacc gcatatgacc ttgagaatct acattagttc ttatatttta
     2101 cagaatgtgt tgaataatga ataaatatgt ataaaaataa cttctggaag aatggttaag
     2161 tagaagctag cagaaataaa aaactttagt atcaaagatc tttattatta aagcataggc
     2221 taccttgtga gtatttaata actgtattgc acaaaaatgt ttccatacag aacccaggat
     2281 tttgttagga ttttttttaa gtcccaattc atgacgaaat ttcaattaca tggttgttga
     2341 aaataaaaag gactgagcca tcatgtgaga aggcagtggg atgctctcag gagttcactg
     2401 ttcctaccac acacctctcg ctgtgggagc cagtgtctgt gtcttgcctg ctgtcctttg
     2461 gtttgacctt gttcaccact actgaaaaga aataatctat caggcctctg tgtatcactt
     2521 ttcttcttct cttcatataa ggcgtgaaaa taggagaata ctgaccaccc aagttccact
     2581 gcaagacctt cccgccgtca gttcagcgtc tgtttgtcta acaatgtatg tgtgtgtctt
     2641 aatgcaatca gaaatatatt tcatttgatc ccattaaaac ttatgtcagt tacatgcttt
     2701 gtccacattt ggtaatacac ttttgcttct tggttgtcca catttggtaa tacatttttg
     2761 cttcttggtc atccactgtc aaataaataa acgtcagctg gccactgtgt taataactg
//
