LOCUS       NM_172857               6042 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus exonuclease 3'-5' domain containing 1 (Exd1),
            transcript variant 2, mRNA.
ACCESSION   NM_172857
VERSION     NM_172857.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Olotu,O., Dowling,M., Homolka,D., Wojtas,M.N., Tran,P.,
            Lehtiniemi,T., Da Ros,M., Pillai,R.S. and Kotaja,N.
  TITLE     Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery
            and exonuclease domain-containing proteins EXD1 and EXD2 in mouse
            spermatocytes
  JOURNAL   Andrology 11 (4), 710-723 (2023)
   PUBMED   36624638
  REMARK    GeneRIF: Intermitochondrial cement (IMC) harbors piRNA biogenesis
            machinery and exonuclease domain-containing proteins EXD1 and EXD2
            in mouse spermatocytes.
REFERENCE   3  (bases 1 to 6042)
  AUTHORS   Pandey,R.R., Homolka,D., Olotu,O., Sachidanandam,R., Kotaja,N. and
            Pillai,R.S.
  TITLE     Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via
            Its Interaction with TDRD12
  JOURNAL   Cell Rep 24 (13), 3423-3432 (2018)
   PUBMED   30257204
  REMARK    GeneRIF: EXD1 enhances MIWI2 piRNA biogenesis via a functional
            interaction with TDRD12.
REFERENCE   4  (bases 1 to 6042)
  AUTHORS   Yang,Z., Chen,K.M., Pandey,R.R., Homolka,D., Reuter,M.,
            Janeiro,B.K., Sachidanandam,R., Fauvarque,M.O., McCarthy,A.A. and
            Pillai,R.S.
  TITLE     PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from
            Cytosolic Targets of the Mouse piRNA Pathway
  JOURNAL   Mol Cell 61 (1), 138-152 (2016)
   PUBMED   26669262
  REMARK    GeneRIF: piRNA biogenesis triggered by PIWI slicing, and promoted
            by EXD1, ensures that the same guides instruct PIWI proteins in the
            nucleus and cytoplasm.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844536.13 and AL844862.12.
            
            On Jun 7, 2022 this sequence version replaced NM_172857.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030132.1, BC150662.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-621               AL844536.13        8645-9265           c
            622-715             AL844536.13        8427-8520           c
            716-901             AL844536.13        2997-3182           c
            902-970             AL844862.12        165024-165092       c
            971-1025            AL844862.12        164123-164177       c
            1026-1153           AL844862.12        163748-163875       c
            1154-1212           AL844862.12        158442-158500       c
            1213-1299           AL844862.12        155089-155175       c
            1300-1404           AL844862.12        153846-153950       c
            1405-1485           AL844862.12        153684-153764       c
            1486-1629           AL844862.12        149572-149715       c
            1630-1821           AL844862.12        148523-148714       c
            1822-6042           AL844862.12        141505-145725       c
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.97 cM"
     gene            1..6042
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /note="exonuclease 3'-5' domain containing 1"
                     /db_xref="GeneID:241624"
                     /db_xref="MGI:MGI:3045306"
     exon            1..621
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            622..715
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            716..901
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..970
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1025
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1153
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1212
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1299
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1404
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1485
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1629
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1821
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..6042
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3117..3122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /note="hexamer: AGTAAA"
     polyA_site      3139
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /note="major polyA site"
     regulatory      3445..3450
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /note="hexamer: ATTAAA"
     polyA_site      3464
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
     regulatory      6018..6023
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
                     /note="hexamer: AATAAA"
     polyA_site      6042
                     /gene="Exd1"
                     /gene_synonym="4932702D22Rik; Exdl1; mExd1"
ORIGIN      
        1 gagcccggaa tgaatcagcc cggaggctgc gggagtccga agggaagcag gccccgcccc
       61 gcggaaccag ggcagccgcc ggacttaaag cggcgatacc gctgtggcgc tagggactgc
      121 acttaaaagg gcagtgcggt ctcgccactg tgttcgaaca aaccagaggc aaactcacta
      181 gggtagccag caggtttttc acaggactca ggtaccagga gtcccaccct acaggtgacg
      241 gagactcagg agagaaacca aacccacgaa acgagggaca aaaccctaag gtggatcccc
      301 agagtagtgc agtgcactct ttcttgctcg ctctccatct ctctgtatct ctctatctct
      361 ttttctccct cctttctcct ctcctctcct ctcactctca ctcccctggt cctaagaata
      421 gggagtccac agccactgtg gcccagcacg cctagagact gcatctttcc tgcgctcctc
      481 cccccggccg cagggggcgc tgagagccgc ctggggtttg catggctcct ggcagcatct
      541 tttctgctct ctctaccccg gccgcagggg gcgctgggaa tgggctcgag gctgcgcgct
      601 gattggctcc tccggaaaga gtagtccctc gttagtttaa ggaactcacc atttgactag
      661 aattaaggtc ggaatttctc aaactccttt gctgtgggga cagaggaccc tgaagggtct
      721 ggatgcttaa agaagactct ttgaaggctg cttctggaac acaaaagcat ggatcccagc
      781 agtgactacc atttcctcaa ccagattttg tggagaaggg tgaagctcac actggtgtct
      841 ggcatctttg agggcgtgct ccagcacgtg gatcctaaca agattgttgt cctgaaaaac
      901 gtgaggaatg cagagtcggg ccgcagcgtc ccaggggtga aagtgttctt cgggcacgag
      961 atcttgaatg tggaactaat ggatgaagca gaaggtgcat cgggggagaa ggcatccgct
     1021 gttagcataa atacagaacg agctggaatg gagaaagtga aaaatgaaga cgtcaatgtc
     1081 tgtgagccgg cttcacctgc accagaggtg ccaaccagct ctctcctcag tgacctcaag
     1141 tactgcccgt cagaggaaga ggaggtgaca tacacagtca ttgatcagtt ccagcagaag
     1201 tttggtgctg caatgctcca catcaagaag cagagtgtcc tgagtgttgc tgccgaaggc
     1261 gccaatgtgt gtcgccatgg gaaactctgt tggcttcagg tggccacaaa cagccgagtt
     1321 tacttgtttg atattttcct tctgggaagt cgtgctttca ataatggact tcagatgatc
     1381 ttagaagaca agagaatctt aaaggttatc cacgattgtc gctggctctc tgactgcctg
     1441 tctcatcagt atggaatcat gctgaacaat gtctttgata cacaggtagc cgacgtcctt
     1501 caattttcta tggaaacggg tggctttctt ccaaactgta tcagtacttt gcaggagagt
     1561 ttaatcagac accttaaagt tgctcccaga taccttttct ttttagaaga gagacagaaa
     1621 cgtattcagg aaaatccaga aatatggttg acaagacctc ttccaccctc tttattgaaa
     1681 atcttggccc tggaaacaac ctacctgctt ccacttcgat tggtgctcct ggatgaggtg
     1741 atgtcagact taaccacact ggtggatggg tacctgaaca cctaccggga gggctctgca
     1801 gaccggcttg caggcacaga gcctgcgtgt atggagctgc cagcagagct tcttcaactc
     1861 caggacttcc agaaacagcg cagagagcga gctgtgaagg agtacagagt gaacgcacgg
     1921 ggcctcctca taaggactcc actgcaccca aaggagccga cagcttgcac agcagggaaa
     1981 gaggagcgag tccagggttt cttattttat aagacggatg gtggagacca agttccacgc
     2041 tttttatgtc ccaaatctca tgaggatgag aagtttttgg ataaagaatc taaacaaacc
     2101 acagcaaagt ctcagattgt gcctcccagg aaggaaggcg aagcccataa ggactctaag
     2161 aacaaaccgg ggtgttggga gtcagcgggg ccagaagacc cgagagccca gaaagcccac
     2221 gccctgcctc ccacgtgggc atctcagtcc cagttttctt tgaaagagga gatagagcag
     2281 ttgacagtgg tgggaaataa gggagcttta acaagcccaa aagaaggcgc tttggtgtcg
     2341 ccttctctcc ttcaggaaac ctgggaggca ccaactgaca ccttccatct tcctgaaaaa
     2401 gctgaggtct ctacacttcc tccctgtcca gccctggaga agacagactc atggataagt
     2461 ccatctttaa atctgtttta ggccaacaga gaccctgtga gtttctcatg gggtcggttg
     2521 gttaggtctc agccacaggc ttctgggcac atggtggcct tagccacagg cttctgggca
     2581 catggtggcc ttaggcttag tgctgaagcc tgctgtctgc attaggaggg gcctaagaag
     2641 agcagtaatg ggtgaggtca aacatgccat tccatcaatg tatgaggaaa tcgtctctgt
     2701 agctgaccca tcttatcttt agctcggact cagtgtctgg aagaatctgg ttcagggatg
     2761 ctgtagggcc gctcttgtgg gtggcagaga gctggggcag ctgcacagac ccccacttaa
     2821 gtctgtaaca tcaccagaaa agaaaaggtg tagggtttta tctgtttggg tttgttttgc
     2881 ccttagattg acgtagatct aaacacatct tacttcccta caggttagaa atgtattctg
     2941 tcaacttcac aagtactgct cttccttaag accttaagtg aactgtgaac ttttgtgttt
     3001 tgtatcaggg tttgggaaca ggtgatgtca ctgtagaggg tatttgctca actttcttta
     3061 tattgcatta atcttaagag agcagggtgc tagcttctct gttgttattt ccttctagta
     3121 aatgtaaaat ttgagttcat tggactgacc tagaggtgtt acacttcctg agagaaaaaa
     3181 aaaaagcaac ctgaaatgag ggcatttgtg ggacgctcac acgtcaataa gcctgctttg
     3241 gcatcagatc atctcttttt ctctttctct ctctctctct ctctctctct ctctttctct
     3301 ctctctctct ctcactctct ctctctctct ctctctttgg tttttcgaga cagggtttct
     3361 ctgtatatcc ctggctgtcc tggaactcac tctatagatc aggctggcca gaaatctgcc
     3421 tgcctctgcc tcccgagtac tgggattaaa ggtgtgcgcc accacgcccg gctctttttc
     3481 tcttttttga gtgagtataa agattttagg gtaatatctg aacttcttct tttttttttt
     3541 aaagaattat ttacttattt tatgtatatg aatacactgt agctgtcttc agacacacca
     3601 gaaaggggtg ttagatctca ttatgggtgg ttgtgagcca ccatatggga attgggattt
     3661 gaactaatga actctggaag agcagtcagt gctcttaacc actgagccat ctctccagcc
     3721 catatctgaa cttgtaggtc tagagtcttg tttagaaatc tgtattctgc cgggcagtgg
     3781 tggcacatgc ctttaatccc agcacttggg aggcagaggc aggcggattt ctgagttcga
     3841 ggccagcctg gtctcgaaac agagaaaccc tgtttcgaaa aaaacagaaa aaaaagaaaa
     3901 gaaaagaaaa ataagaaatc tgtattctac ctagatacag tgatgcatat ctctaaggcc
     3961 agcactcagg aggttagaca gagatggatg ggcatttgag gccaacttag gcctcaatct
     4021 ccagttatag gactgtcggt gcaccttggg gatacaggga cagtccagaa ttagcattgg
     4081 acaaccaatt gttttatgac tgtctcagat tttcagaacc aaatcatgac atagtggcca
     4141 tccctgtaat cccagcactc tcatgagagg gcaaagcaag agaatccaaa actcaaggcc
     4201 gttctcagct gtgtactgtg tcccacgcgg gcacaggaga cctatttcaa aaatcaaact
     4261 aaatgaacta ctcttttttt tctttaaaga tttatttatt tattatatat gtctacattg
     4321 tagctgtcct cagacactcc agaagagggc atcagatttc gttacggatg gttgtgagcc
     4381 accatgtggg tgctgggatt tgaactcagg accttcgaaa gagcagttgg tgctcttaac
     4441 tgctgagcca tctctccagc ccatgagagg ggattttaaa atgaaagttt tcaaattttt
     4501 tattttctgt gtatgagtgt tctgcctgca tgtattatct gtacaccatg tgctctcaga
     4561 aaccatggga agaccaggca gtggtggcac acaactttga tctagggctt gggaggcaag
     4621 acagatctct aagtttaagg ccaggctggt ctacagagtg agtttcagga cagccagggc
     4681 tacagaggga aaaaggaagc agaggcagac agtggacccc ccaaccctaa acaagacatt
     4741 tctaccattt cctctgaagc tcagggaacg tggcagaaca ggacatagaa ggatgagcca
     4801 gggactacag ggctacaaat acctttttct ggacaagaca gagccattgc atcacaaatt
     4861 tacaacagct gcaaatgact acacaagaga gggctgtcac ttaaagtaag aaaaaagatc
     4921 cacatgcctt tgaatcccag tgaatctgag tttgaggcta gcctggtcta cagagcaagt
     4981 tccaggacgt ccaggactac acagagagat cctgtcttga aaaagaaacc aatatctgag
     5041 taaggctgag cctgtcagtg aagcctgggt ggaggagggc ctcaggccta cctctccgtg
     5101 ctgacagatt gagtgggcca ggaaccacta ccgttagtac acaagcaatg tcacaaatga
     5161 cagtgacccc atcaggctcc ccggtgggta gttccaaacc agaggtcata cagttgaccc
     5221 tggttaaact caaaaaaggc caaaagctat gaatctgtta aaggagctgg taaggatgag
     5281 agaaggtgtg tgtgtatgaa tcacaatacc ttaaatgaca ctgtcaaaga acagaatcaa
     5341 taaagtgatg tatggatggc atccagacaa gctgcccagt cagtccataa ctgctaatcc
     5401 accatccctt ccctcctctc ccttcccgga ttgctggccc tcggggctct tgcccttgct
     5461 ttgttgttat tacagagttc ttctttaaag tacttcagct ttagctgtca ggtggctttc
     5521 aagttgtaaa cagtgatgca cttgggggaa tttgcgagct ccagaatgca gaagtagtca
     5581 ctgaggctct gtggcggcat cagagcccca ggcttcgctc actggcctct agtttacact
     5641 gtcatcagat gatcccagca gctgtttaca tctaacagct gtcactgttg ctcctaaaga
     5701 aaaggaagag ggggattcaa aattaaaatt ttatggcaac aaaaagagag gatgaaatgg
     5761 gctgcaggta ggactctagt tactgcttag atgctgttct ctttgcagat tatttacttc
     5821 tttatatgtg tagggttgtt gtgtatgtaa gtcagtgtac cccatgcacg tagtacttag
     5881 ggaggccaga gaagagcact ggctctgcta ttgctggagt cgtggatgct ggaaaggtct
     5941 cctggaagag tagccaatac tcgtaactgc taatcagtct ctccagctgc cacccattgt
     6001 cttcttgctg cattctgaat aaacattttc ttttttaaaa aa
//
