LOCUS       NM_007968               2436 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Ewing sarcoma breakpoint region 1 (Ewsr1), transcript
            variant 2, mRNA.
ACCESSION   NM_007968 XM_988357
VERSION     NM_007968.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Jung,J.W., Kim,H., Park,J., Woo,J., Jeon,E., Lee,G., Park,M.,
            Kim,S., Seo,H., Cheon,S., Dan,K., Lee,J., Ryu,H. and Han,D.
  TITLE     In-depth proteome analysis of brain tissue from Ewsr1 knockout
            mouse by multiplexed isobaric tandem mass tag labeling
  JOURNAL   Sci Rep 13 (1), 15261 (2023)
   PUBMED   37709831
  REMARK    GeneRIF: In-depth proteome analysis of brain tissue from Ewsr1
            knockout mouse by multiplexed isobaric tandem mass tag labeling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2436)
  AUTHORS   Wang,Y., Feswick,A., Apostolou,V., Petkov,P.M., Moser,E.K. and
            Tibbetts,S.A.
  TITLE     Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative
            regulator of B cell responses
  JOURNAL   Proc Natl Acad Sci U S A 119 (32), e2123362119 (2022)
   PUBMED   35921433
  REMARK    GeneRIF: Gammaherpesvirus-mediated repression reveals EWSR1 to be a
            negative regulator of B cell responses.
REFERENCE   4  (bases 1 to 2436)
  AUTHORS   Verdile,V., Svetoni,F., La Rosa,P., Ferrante,G., Cesari,E.,
            Sette,C. and Paronetto,M.P.
  TITLE     EWS splicing regulation contributes to balancing Foxp1 isoforms
            required for neuronal differentiation
  JOURNAL   Nucleic Acids Res 50 (6), 3362-3378 (2022)
   PUBMED   35253879
REFERENCE   5  (bases 1 to 2436)
  AUTHORS   Tian,H. and Petkov,P.M.
  TITLE     Mouse EWSR1 is crucial for spermatid post-meiotic transcription and
            spermiogenesis
  JOURNAL   Development 148 (11) (2021)
   PUBMED   34100066
  REMARK    GeneRIF: Mouse EWSR1 is crucial for spermatid post-meiotic
            transcription and spermiogenesis.
REFERENCE   6  (bases 1 to 2436)
  AUTHORS   Eliazer,S., Spencer,J., Ye,D., Olson,E. and Ilaria,R.L. Jr.
  TITLE     Alteration of mesodermal cell differentiation by EWS/FLI-1, the
            oncogene implicated in Ewing's sarcoma
  JOURNAL   Mol Cell Biol 23 (2), 482-492 (2003)
   PUBMED   12509448
  REMARK    GeneRIF: EWS has a role in mesodermal cell differentiation
REFERENCE   7  (bases 1 to 2436)
  AUTHORS   Sutherland,H.G., Mumford,G.K., Newton,K., Ford,L.V., Farrall,R.,
            Dellaire,G., Caceres,J.F. and Bickmore,W.A.
  TITLE     Large-scale identification of mammalian proteins localized to
            nuclear sub-compartments
  JOURNAL   Hum Mol Genet 10 (18), 1995-2011 (2001)
   PUBMED   11555636
REFERENCE   8  (bases 1 to 2436)
  AUTHORS   Melot,T., Dauphinot,L., Sevenet,N., Radvanyi,F. and Delattre,O.
  TITLE     Characterization of a new brain-specific isoform of the EWS
            oncoprotein
  JOURNAL   Eur J Biochem 268 (12), 3483-3489 (2001)
   PUBMED   11422378
REFERENCE   9  (bases 1 to 2436)
  AUTHORS   Mencinger,M. and Aman,P.
  TITLE     Characterization of TFG in mus musculus and Caenorhabditis elegans
  JOURNAL   Biochem Biophys Res Commun 257 (1), 67-73 (1999)
   PUBMED   10092511
REFERENCE   10 (bases 1 to 2436)
  AUTHORS   Plougastel,B., Mattei,M.G., Thomas,G. and Delattre,O.
  TITLE     Cloning and chromosome localization of the mouse Ews gene
  JOURNAL   Genomics 23 (1), 278-281 (1994)
   PUBMED   7829090
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046434.1, BY272748.1, AK151625.1, CB205713.1 and AI852718.1.
            
            On Apr 21, 2018 this sequence version replaced NM_007968.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK034755.1, AK151625.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AK046434.1         2-9
            9-37                BY272748.1         1-29
            38-2208             AK151625.1         1-2171
            2209-2269           CB205713.1         535-595
            2270-2436           AI852718.1         1-167               c
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.22 cM"
     gene            1..2436
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="Ewing sarcoma breakpoint region 1"
                     /db_xref="GeneID:14030"
                     /db_xref="MGI:MGI:99960"
     exon            1..88
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..2043
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="isoform 2 is encoded by transcript variant 2; Ewing
                     sarcoma homolog; RNA-binding protein EWS"
                     /codon_start=1
                     /product="RNA-binding protein EWS isoform 2"
                     /protein_id="NP_031994.2"
                     /db_xref="CCDS:CCDS24396.1"
                     /db_xref="GeneID:14030"
                     /db_xref="MGI:MGI:99960"
                     /translation="MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTY
                     GQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPTAPQAYSQPVQGYGTGTYDSTT
                     ATVTTTQASYAAQTAYGTQPAYPTYGQQPTATAPTRPQDGNKPAETSQPQSSTGGYNQ
                     PSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPS
                     SYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMG
                     VYGQESGGFSGPGENRSLSGPDNRGRGRGGFDRGGMSRGGRGGGRGGLGAGERGGFNK
                     PGGPMDEGPDLDLGLPIDPDEDSDNSAIYVQGLNDNVTLDDLADFFKQCGVVKMNKRT
                     GQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPM
                     NSMRGGMPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNP
                     SGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGG
                     PGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPL
                     MEQMGGRRGGRGGPGKMDKGEHRQERRDRPY"
     exon            89..125
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            126..177
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            178..301
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            302..488
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            489..656
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            657..868
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1049
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1084
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1117
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1236
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..1366
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1489
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1490..1652
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1750
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..2003
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2420
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2391..2396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="hexamer: AATAAA"
     polyA_site      2420
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="major polyA site"
ORIGIN      
        1 gcctgcgcag tgcggcgcct agggggaacg cgagaaggag acggacgttg agagaacgag
       61 gaggaaggcg agaaaatggc gtccacggat tacagtacct atagtcaagc tgcagcccag
      121 cagggctaca gtgcttacac cgcccagcca actcaaggat atgcacagac cacccaggca
      181 tatgggcaac aaagctatgg aacctatgga cagcctactg atgtcagcta tactcaggct
      241 cagaccactg ccacctacgg gcagactgca tatgcaactt cttacggaca gcctcccact
      301 ggttatagca ctccaactgc cccgcaggcg tacagccagc ctgtgcaggg atatggcact
      361 gggacttatg acagcaccac tgctacagtc accacaacgc aggcctctta cgcagctcag
      421 actgcatatg gcacccagcc tgcctacccc acctatggcc agcagccaac agccaccgca
      481 cctaccagac cacaggatgg taacaagcct gctgagacta gtcaacctca atctagcaca
      541 gggggttata accaacccag cctaggatat ggacagagta actacagcta tccccaggta
      601 cctgggagct acccaatgca gccagtcacc gcacctccat cttatcctcc taccagctac
      661 tcctcttcac agccgactag ttacgatcag agcagttact ctcagcagaa cacctatggg
      721 cagccgagca gctatggaca acagagtagc tatggtcaac aaagcagcta tgggcagcag
      781 cctcctacta gttacccccc tcagactgga tcctacagcc aggctccaag tcaatatagc
      841 caacagagca gcagctacgg gcagcagagt tcattccgac aggaccaccc cagtagcatg
      901 ggtgtttatg ggcaggagtc tggaggattt tccggaccag gagagaaccg gagcttgagt
      961 ggccctgata accggggcag gggaagaggg ggatttgatc gtggaggcat gagcagaggt
     1021 gggcggggag gaggacgcgg tggactgggc gctggagagc gaggtggctt caataagcct
     1081 ggtggaccca tggatgaagg accagatctt gatctaggcc ttcctataga tcccgatgaa
     1141 gactctgaca acagtgcaat ttatgtgcaa ggattaaatg acaatgtgac tctggatgat
     1201 ctggcagact tctttaagca gtgtggggtt gtcaagatga acaagagaac tggacaaccc
     1261 atgatccata tctacctgga taaggagaca ggaaagccta aaggggacgc cacagtgtcc
     1321 tatgaagatc caccaactgc aaaggctgcc gtggaatggt ttgatgggaa agattttcaa
     1381 ggaagcaaac ttaaagtgtc tcttgcccga aagaagcctc caatgaacag catgcgggga
     1441 ggcatgccac ctcgtgaggg cagggggatg ccaccaccac ttcgtggagg tcctggtggc
     1501 ccaggaggcc ctggaggacc catgggtcgc atgggaggcc gtggaggaga cagagggggc
     1561 ttccctccaa gagggccccg aggctccaga ggaaacccct ctggaggagg aaatgtccag
     1621 caccgagctg gagactggca gtgtcccaat ccgggctgtg gaaaccagaa cttcgcttgg
     1681 agaacagaat gcaaccagtg taaggcccct aagcccgagg gcttcctccc gccacccttt
     1741 ccacctccgg gtggtgatcg tggacgaggt ggccctggtg gcatgcgagg aggaagagga
     1801 ggactcatgg accgtggtgg tcctggagga atgttcagag gtggcagagg tggagacaga
     1861 ggaggcttcc gaggtggccg tggaatggac cgaggtggct ttggtggagg aagacgaggt
     1921 ggtcctgggg ggcctcctgg acctttaatg gaacagatgg gaggaagaag aggcggacgt
     1981 ggaggacctg ggaaaatgga taaaggcgag caccgtcagg aacgcagaga ccggccctac
     2041 tagagacctg cagagctgca ttgacgacca gatttatttt ttaaaccaga aaatgtttta
     2101 aatttataat tccatattta taatgttggc gacaacatta tgattattcc ttgtctgtac
     2161 tttagtattt tcaccatttg tgaagaaaca ttaaaacaag ttaaatagta gtgtgctgag
     2221 tttttttttg ttgttgttgt tttttgattt gttctttttt ttttcctttt taagatggtt
     2281 gttttaagac ttaacaatgg gaaccccttg tgagcatgct cagtatcatt gtggagaacc
     2341 aagagggcct ctaattgtaa caatgttcat ggttgtgatt ttttttttta aataaaattc
     2401 caaatgttta taaactgtca aaaaaaaaaa aaaaaa
//
