LOCUS       NM_001283063            3224 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus Ewing sarcoma breakpoint region 1 (Ewsr1), transcript
            variant 4, mRNA.
ACCESSION   NM_001283063
VERSION     NM_001283063.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Jung,J.W., Kim,H., Park,J., Woo,J., Jeon,E., Lee,G., Park,M.,
            Kim,S., Seo,H., Cheon,S., Dan,K., Lee,J., Ryu,H. and Han,D.
  TITLE     In-depth proteome analysis of brain tissue from Ewsr1 knockout
            mouse by multiplexed isobaric tandem mass tag labeling
  JOURNAL   Sci Rep 13 (1), 15261 (2023)
   PUBMED   37709831
  REMARK    GeneRIF: In-depth proteome analysis of brain tissue from Ewsr1
            knockout mouse by multiplexed isobaric tandem mass tag labeling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3224)
  AUTHORS   Wang,Y., Feswick,A., Apostolou,V., Petkov,P.M., Moser,E.K. and
            Tibbetts,S.A.
  TITLE     Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative
            regulator of B cell responses
  JOURNAL   Proc Natl Acad Sci U S A 119 (32), e2123362119 (2022)
   PUBMED   35921433
  REMARK    GeneRIF: Gammaherpesvirus-mediated repression reveals EWSR1 to be a
            negative regulator of B cell responses.
REFERENCE   4  (bases 1 to 3224)
  AUTHORS   Verdile,V., Svetoni,F., La Rosa,P., Ferrante,G., Cesari,E.,
            Sette,C. and Paronetto,M.P.
  TITLE     EWS splicing regulation contributes to balancing Foxp1 isoforms
            required for neuronal differentiation
  JOURNAL   Nucleic Acids Res 50 (6), 3362-3378 (2022)
   PUBMED   35253879
REFERENCE   5  (bases 1 to 3224)
  AUTHORS   Tian,H. and Petkov,P.M.
  TITLE     Mouse EWSR1 is crucial for spermatid post-meiotic transcription and
            spermiogenesis
  JOURNAL   Development 148 (11) (2021)
   PUBMED   34100066
  REMARK    GeneRIF: Mouse EWSR1 is crucial for spermatid post-meiotic
            transcription and spermiogenesis.
REFERENCE   6  (bases 1 to 3224)
  AUTHORS   Eliazer,S., Spencer,J., Ye,D., Olson,E. and Ilaria,R.L. Jr.
  TITLE     Alteration of mesodermal cell differentiation by EWS/FLI-1, the
            oncogene implicated in Ewing's sarcoma
  JOURNAL   Mol Cell Biol 23 (2), 482-492 (2003)
   PUBMED   12509448
  REMARK    GeneRIF: EWS has a role in mesodermal cell differentiation
REFERENCE   7  (bases 1 to 3224)
  AUTHORS   Sutherland,H.G., Mumford,G.K., Newton,K., Ford,L.V., Farrall,R.,
            Dellaire,G., Caceres,J.F. and Bickmore,W.A.
  TITLE     Large-scale identification of mammalian proteins localized to
            nuclear sub-compartments
  JOURNAL   Hum Mol Genet 10 (18), 1995-2011 (2001)
   PUBMED   11555636
REFERENCE   8  (bases 1 to 3224)
  AUTHORS   Melot,T., Dauphinot,L., Sevenet,N., Radvanyi,F. and Delattre,O.
  TITLE     Characterization of a new brain-specific isoform of the EWS
            oncoprotein
  JOURNAL   Eur J Biochem 268 (12), 3483-3489 (2001)
   PUBMED   11422378
REFERENCE   9  (bases 1 to 3224)
  AUTHORS   Mencinger,M. and Aman,P.
  TITLE     Characterization of TFG in mus musculus and Caenorhabditis elegans
  JOURNAL   Biochem Biophys Res Commun 257 (1), 67-73 (1999)
   PUBMED   10092511
REFERENCE   10 (bases 1 to 3224)
  AUTHORS   Plougastel,B., Mattei,M.G., Thomas,G. and Delattre,O.
  TITLE     Cloning and chromosome localization of the mouse Ews gene
  JOURNAL   Genomics 23 (1), 278-281 (1994)
   PUBMED   7829090
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046434.1, BY272748.1 and AK147909.1.
            
            On Apr 21, 2018 this sequence version replaced NM_001283063.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147909.1, AK146791.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AK046434.1         2-9
            9-51                BY272748.1         1-43
            52-3224             AK147909.1         1-3173
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 3.22 cM"
     gene            1..3224
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="Ewing sarcoma breakpoint region 1"
                     /db_xref="GeneID:14030"
                     /db_xref="MGI:MGI:99960"
     exon            1..88
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1053
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="isoform 4 is encoded by transcript variant 4; Ewing
                     sarcoma homolog; RNA-binding protein EWS"
                     /codon_start=1
                     /product="RNA-binding protein EWS isoform 4"
                     /protein_id="NP_001269992.1"
                     /db_xref="GeneID:14030"
                     /db_xref="MGI:MGI:99960"
                     /translation="MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTY
                     GQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYSTPTAPQAYSQPVQGYGTGTYDSTT
                     ATVTTTQASYAAQTAYGTQPAYPTYGQQPTATAPTRPQDGNKPAETSQPQSSTGGYNQ
                     PSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSSQPTSYDQSSYSQQNTYGQPS
                     SYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMG
                     VYGQESGGFSGPGENRSLSGPDNRGRGRGGFDRGGMSRGGRGGGRGGLG"
     exon            89..125
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            126..177
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            178..301
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            302..488
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            489..656
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            657..868
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     exon            869..3224
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2859..2864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="hexamer: ATTAAA"
     polyA_site      2888
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="major polyA site"
     regulatory      3138..3143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
                     /note="hexamer: TATAAA"
     polyA_site      3155
                     /gene="Ewsr1"
                     /gene_synonym="Ews; Ewsh"
ORIGIN      
        1 gcctgcgcag tgcggcgcct agggggaacg cgagaaggag acggacgttg agagaacgag
       61 gaggaaggcg agaaaatggc gtccacggat tacagtacct atagtcaagc tgcagcccag
      121 cagggctaca gtgcttacac cgcccagcca actcaaggat atgcacagac cacccaggca
      181 tatgggcaac aaagctatgg aacctatgga cagcctactg atgtcagcta tactcaggct
      241 cagaccactg ccacctacgg gcagactgca tatgcaactt cttacggaca gcctcccact
      301 ggttatagca ctccaactgc cccgcaggcg tacagccagc ctgtgcaggg atatggcact
      361 gggacttatg acagcaccac tgctacagtc accacaacgc aggcctctta cgcagctcag
      421 actgcatatg gcacccagcc tgcctacccc acctatggcc agcagccaac agccaccgca
      481 cctaccagac cacaggatgg taacaagcct gctgagacta gtcaacctca atctagcaca
      541 gggggttata accaacccag cctaggatat ggacagagta actacagcta tccccaggta
      601 cctgggagct acccaatgca gccagtcacc gcacctccat cttatcctcc taccagctac
      661 tcctcttcac agccgactag ttacgatcag agcagttact ctcagcagaa cacctatggg
      721 cagccgagca gctatggaca acagagtagc tatggtcaac aaagcagcta tgggcagcag
      781 cctcctacta gttacccccc tcagactgga tcctacagcc aggctccaag tcaatatagc
      841 caacagagca gcagctacgg gcagcagagt tcattccgac aggaccaccc cagtagcatg
      901 ggtgtttatg ggcaggagtc tggaggattt tccggaccag gagagaaccg gagcttgagt
      961 ggccctgata accggggcag gggaagaggg ggatttgatc gtggaggcat gagcagaggt
     1021 gggcggggag gaggacgcgg tggactgggg taagagcaaa accttttctc cttttatcta
     1081 attttgtttc atccatagga ttttcaatgg aaagaaggga ctgaaagaca taagaaattt
     1141 atcccacatt tccatggaca atctattgcg gtcaagctat ctcctaaaac atggaaatgt
     1201 catttaagtg gcagtttgct tttttccctg ccagtaacca ttgttgggct ggggtgaaca
     1261 aagaatggct ttgaaactag agctttcagt ccccttcatg gttttcagag gtgagagcct
     1321 ctttgggtaa tagatttggg atggaacaca cacaaccctt ctaataaccc tagcttttaa
     1381 ctaatgtcct gaacggttgt cttgaaatac ctggtgtatg tactgcagaa aaggaatgca
     1441 gctgttttcc aatgtttgta cccagatttg ttgatcatga tgaagactga ctgacctcat
     1501 gacccttaaa ccatggcctg aggcgccccg atgtgtagtt aagttagagc tgagaacaac
     1561 cgtaatagcc ttgttacaga gtaatgtata tgaatatagc atcgctcgct attggagatt
     1621 ttaaaagtct aaattggttt gaccattgtg attgatggaa caatctggtt tagaaaactg
     1681 tttagatcag aaccatgacc ccgctgcctc tgcctcccct tccctcccct ccacccctct
     1741 tccctttctg gtgtctgtac tttgatgaag gtgaagtatg atgttggtgg ttaacataat
     1801 cagatttagt caatgtgtgt gttctgtccc tatataaact agtaaaacaa agttatgcag
     1861 aatgttgtag tgagtataca tttgattaac tgttaaagaa aaactagcaa ttctttttta
     1921 tagaacaaac ttcaacacag attaagttga cctattcatt tattagtaaa tttctgtact
     1981 actgaaagtt agtattttgt ttattctgca taactttaat aaagtctcca gtgaaatctt
     2041 tattggaaaa aagtgtttat aaaaatacat gcaaattttt atgaataatt tttgacaatg
     2101 taaaattggt cttcagattt ggagaatatc attccattaa taaaggtttc acgtctttat
     2161 ggatttttaa aaatagacat agacacttga aattactatc atcacgtctc aaatcaccta
     2221 gaattcttag tatttttcaa ttgcaaaagc aatttgaagt ttattcatct taatactcca
     2281 gaagtgatga ttctaccgac tttgttagat tgctattaga ggggcccttg tttgtggatg
     2341 agaagtctct cctggagcca tagaagagac catggcctac cccataggat tttaacagaa
     2401 ctgtgaagca gtctgttgga tcctgtgagg acaggacact gggcagtccc ctttcccttt
     2461 ttttattgtg gtgatggttt tcagtgtctg ctgcaggtaa ggcgctcaat gttgttaaca
     2521 tgcccttttt cattgcctca gtgtttgata tttttcattg gctgttaaac gtggaaactt
     2581 tttaacatgc tttgtcgcat ttatatgcta caggttacaa agtgagagcc ttgtatacac
     2641 ttcaatactt aaaaagtacc cgtactcagt actcagccgg cagcataatg aaaagtggga
     2701 ctagacacgg tgtccatatg gagaggaaaa atatacatag aatattttaa acaaaatgta
     2761 ttcattgtat aaatggaatc cttctgtaac tttggtaact gcatacttgt tgtttggtaa
     2821 tgaaccagag gaggtataat actctagaat tgtgtaacat taaagtgtaa actttgtgtt
     2881 taaagagaga gagagcattt tatggtgtgt acttttaaca aggagtaaag ctgcatgcaa
     2941 cagcttggac tgtgtatcta ggtattaaag gtgcaatact ggttaggaga gagcctcagg
     3001 gctgccgact ggggagctgc ctctattcac tactagaaaa gactgccttc ccctccctcc
     3061 ccctcaaaaa aggccacaat ctattccctc tcctctgtgt atagtatgta tattgccgta
     3121 gtcttaactg gtatgtctat aaagttgcac ccccagtccg ctgagccttg gtatttattg
     3181 taaattctcc tttcacagta actattaaat ccattagggc cctg
//
