LOCUS       NM_146023               3964 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus ecotropic viral integration site 2 (Evi2), mRNA.
ACCESSION   NM_146023
VERSION     NM_146023.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Church,D.M., Goodstadt,L., Hillier,L.W., Zody,M.C., Goldstein,S.,
            She,X., Bult,C.J., Agarwala,R., Cherry,J.L., DiCuccio,M.,
            Hlavina,W., Kapustin,Y., Meric,P., Maglott,D., Birtle,Z.,
            Marques,A.C., Graves,T., Zhou,S., Teague,B., Potamousis,K.,
            Churas,C., Place,M., Herschleb,J., Runnheim,R., Forrest,D.,
            Amos-Landgraf,J., Schwartz,D.C., Cheng,Z., Lindblad-Toh,K.,
            Eichler,E.E. and Ponting,C.P.
  CONSRTM   Mouse Genome Sequencing Consortium
  TITLE     Lineage-specific biology revealed by a finished genome assembly of
            the mouse
  JOURNAL   PLoS Biol 7 (5), e1000112 (2009)
   PUBMED   19468303
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Trost,M., English,L., Lemieux,S., Courcelles,M., Desjardins,M. and
            Thibault,P.
  TITLE     The phagosomal proteome in interferon-gamma-activated macrophages
  JOURNAL   Immunity 30 (1), 143-154 (2009)
   PUBMED   19144319
REFERENCE   3  (bases 1 to 3964)
  AUTHORS   Zanivan,S., Gnad,F., Wickstrom,S.A., Geiger,T., Macek,B., Cox,J.,
            Fassler,R. and Mann,M.
  TITLE     Solid tumor proteome and phosphoproteome analysis by high
            resolution mass spectrometry
  JOURNAL   J Proteome Res 7 (12), 5314-5326 (2008)
   PUBMED   19367708
REFERENCE   4  (bases 1 to 3964)
  AUTHORS   Villen,J., Beausoleil,S.A., Gerber,S.A. and Gygi,S.P.
  TITLE     Large-scale phosphorylation analysis of mouse liver
  JOURNAL   Proc Natl Acad Sci U S A 104 (5), 1488-1493 (2007)
   PUBMED   17242355
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591174.13 and AK034440.1.
            
            On May 15, 2013 this sequence version replaced NM_146023.3.
            
            Summary: This locus represents naturally occurring readthrough
            transcription between the neighboring Evi2a (ecotropic viral
            integration site 2a) and Evi2b (ecotropic viral integration site
            2b) genes on chromosome 11. The readthrough transcript encodes a
            protein identical to that of the downstream gene product. [provided
            by RefSeq, May 2013].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK034440.1, AK156127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164140 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 14017, 216984
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AL591174.13        171031-171080
            51-3964             AK034440.1         2-3915
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11"
     gene            1..3964
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="ecotropic viral integration site 2"
                     /db_xref="GeneID:101488212"
                     /db_xref="MGI:MGI:5439444"
     exon            1..241
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /inference="alignment:Splign:2.1.0"
     exon            242..462
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /inference="alignment:Splign:2.1.0"
     exon            463..586
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /inference="alignment:Splign:2.1.0"
     exon            587..3964
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /inference="alignment:Splign:2.1.0"
     CDS             602..1936
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="ecotropic viral integration site 2B protein;
                     protein EVI2B; Evi2a-Evi2b readthrough"
                     /codon_start=1
                     /product="protein EVI2B precursor"
                     /protein_id="NP_666135.1"
                     /db_xref="GeneID:101488212"
                     /db_xref="MGI:MGI:5439444"
                     /translation="MEFKYLVFIVLCQYLDNTFFSETEAITTEQQSLSTLITPSLYVT
                     TDSQNTAGNALSQTTRFKNISSGQQASPAQITPEQATPAVYVSSSPLTYNITRQAESA
                     VNNSLPQTSPSGFTLTNQPSPSTYNSTGQPPKHLVYTSTQQPPSPAPTSSGKPEVEST
                     HNQPTKSTPTIYLQRDTPPPPPPPLTSEPPSGKGTAHKNNHNAIAAILIGTIIISMLV
                     AILMIILWKYLRKPVLNDQNWAGRSPFADGETPEMCMDNIRESEASTKRASVVSLMTW
                     KPSKSTLLADDLEVKLFESSEHINDTSNLKTDNVEVQINGLSEDSADGSTVGTAVSSD
                     DADLALPPPLLDLDENLPNKPTVTVVSPLPNDSINPQPSPDGLNQVCEEQHSKIQEPF
                     PPPPDSFNVPLSAGDFINNQESAHEAQCQEFSTPDLHPDLTDSLPPPPTELL"
     regulatory      2269..2274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="hexamer: AATAAA"
     polyA_site      2297
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="major polyA site"
     regulatory      2308..2313
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="hexamer: AATAAA"
     polyA_site      2333
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
     regulatory      2496..2501
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="hexamer: ATTAAA"
     polyA_site      2519
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
     regulatory      3589..3594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
                     /note="hexamer: AATAAA"
     polyA_site      3612
                     /gene="Evi2"
                     /gene_synonym="Evi2a-evi2b; Gm21975"
ORIGIN      
        1 ttgtcatccc attgccagct ggacaagtca gtctttgctt tccccgtgac atgtagttga
       61 gttgcagcgg aaaggaaatg tgtcatctgt ggtttggttt taagagtgga aagctagctg
      121 tacatatcct ctttaaaaaa ccctgcagat ttaagtttca cagtctccaa agtattctac
      181 ttaaaataag aagagcagaa ctgaggtggc ttacgaagtg acggctgggc gagctctgaa
      241 gatagcagtg gacctgtagg tatggagcac aaaggacagt acctgcatct tgttttcctg
      301 atgacaacag tgtgggcctc gtcgtcttct ggaacaagac caaactacac acacctgtgg
      361 gctagcagtg tcactgcctc aggttccagt aatcagaatg gctccagcag acatccgagt
      421 gacaataaca caaaccttgt cactcctgcc gtgggtcaca agaagccgtc tcagtgcgct
      481 ggagaccacc gttaatccgc actgaaacac ccctttgctc tctcagccaa atcaccaaca
      541 tgtcctgtta gaacaagaat ttacattctg cgaaagaagt tcacaggaga aaacatctga
      601 aatggaattc aagtatctgg tcttcattgt gctttgtcaa tacctggaca atacgttttt
      661 ctcagagaca gaagcaatta caacagagca gcaatcactg tctactttaa tcacaccgtc
      721 gttatatgtt acaactgatt ctcaaaacac agcagggaat gctttgagtc agacaacaag
      781 attcaagaac atttcttctg gacagcaagc atcacctgcc caaatcactc ctgaacaagc
      841 aacaccagct gtttatgtct cttcaagccc acttacttat aacattacca gacaagcaga
      901 atcagcggtc aacaactcct tgcctcaaac atcaccatct gggttcactt tgaccaatca
      961 gccatcacct tctacctata attctactgg acaaccacca aaacatcttg tctatacttc
     1021 cacacaacag ccaccatcac ctgctcctac ctcttctgga aaaccagaag tagagtctac
     1081 tcataatcag cccacaaaat caacaccaac tatttattta caaagggaca caccaccacc
     1141 accaccacca ccactcactt cagaaccacc aagtggcaaa ggaactgctc ataaaaacaa
     1201 ccacaatgca attgctgcca tattaatcgg tactattata atttctatgt tggtagctat
     1261 actcatgatt atactgtgga aatacttgag gaagccagtc ttaaatgatc aaaactgggc
     1321 aggtaggtct ccatttgctg atggagaaac accagaaatg tgtatggata acatcagaga
     1381 gagtgaggca tccacaaagc gtgcatcagt tgtctcactt atgacctgga aaccaagtaa
     1441 aagcacactg ctagcagatg atttagaagt taagttgttt gaatcaagtg aacacattaa
     1501 tgataccagc aacctcaaga ctgataatgt agaagttcaa ataaatggct tatcagaaga
     1561 cagtgctgat gggtcaacag ttggtacagc cgtgtcttca gatgatgcag atctggccct
     1621 gccacctccc cttcttgatt tggatgagaa cctaccaaac aagcccacag tgacagttgt
     1681 atctccttta ccaaatgatt ctatcaatcc ccaaccatct ccagatggtc taaatcaagt
     1741 gtgtgaagaa cagcattcca agatccaaga gccattcccg ccaccccctg actcatttaa
     1801 tgtgcctctg tcagcaggag attttataaa caatcaagag tcggcccacg aggctcaatg
     1861 ccaggagttc tctactcctg acctccatcc agacctcaca gactccctgc cacctccacc
     1921 tacagagctg ctgtaaagtg acagctggct ttctagactg ccctccatcc tgagatggca
     1981 ccgacacgtt cctgtcttcg tggtatggca cacaggacat gacacctggc acctgagaga
     2041 ctctttaggg actacctgac ccctgactga agcccacgta cacgggtaga ttattacagt
     2101 ggtatgctta ccagctacag aagtaatttt taaatcagag tgccttttat atatgttaat
     2161 gatagatatt tcaataactt aatcactttg gaaacatatg atctacatat acttattatt
     2221 acccaataga tttcccccga aaagctgtat ataaacatat cttaaataaa taaaattcta
     2281 ttttttccac ctcaaaattt ggggtataat aaaaatggaa atttttaaat ggtcaaagtt
     2341 ttaatacttc aaatattaag taaatatttt catttataga gattaaacac acagctacca
     2401 aactgtatta aaacatgaag tctgagtgct gtgtttggga tacatttacc ctgaaaaagt
     2461 gagcacttac cataaaattt tcttttttct ttataattaa aaattttatg tatataagag
     2521 ttttgcctga atatgtgtgt atatattcat atagtcacac acacacacat atatataaga
     2581 cagaagatgg cactactaac atttgccaat gccccgtgag tcctggaact gactccaagt
     2641 cctctgtaag tgctcttaac caccgagccc ctgcaactcc gttcttcccc tcctcttcct
     2701 cctcctcctc ttcctcctcc tcttcctctt caaatgtttg gggcgtgggg gtggaggtca
     2761 aagagatagg ctgtatttgg ggttagaaga tgacttgtgg gagttgattt ttttcttcca
     2821 cctcaaggat aaaattcaga actcaagtca tgagacctga cagcaagcaa cctttatcct
     2881 ctaagcccat ctcactgccc ccaaaatgta aacatttttc taaaatgtta gggaagcata
     2941 aactaaccac ttagacaaaa atcaaatgat atggaagacg ggagaaaaaa atagaagtgg
     3001 gctgtatctg tgtgtctctt cccacacatg catgatacca gcctgtgtga gggtgtctgt
     3061 ggagaccaca gaggatacca gccatcctgg aactgaagtc ccaggaagtt atgagctgct
     3121 gtctaacacg ggtactggga actgaacttg ggtcctatag gacagcagca agttctctaa
     3181 agtactgacc catcactgca gccccagcct ccttttgtaa atgcaggaat gtaatcatca
     3241 cctgaaggtt gttgagtatg aggcactcag aacagaagtg ggcacaactt ttgttaaagt
     3301 ggaggcggca gtgggtactg gcctaccttt tgcagatgtc aaatgttcat ctgcccaaca
     3361 agccatcctt ctcttattat tattacaagt ccaagaaagg acagttgctt agagcacaga
     3421 ttttggcact tttcaagata gtatatagta tttattggta acattaatgt tcaaatacta
     3481 aaaatcccat gtagaataaa aacaagaagg ttctcgggca gtggagtttt actactactg
     3541 ctactattac tactacatac tactactatt atttagtata taattatcaa taaacaatta
     3601 ttatacatat tataaataca tgtaaattta ttattgttgt tgtttgtgtt ctgagacagg
     3661 agtcctctgg tagccctggc tgtcctggca ctctctctct atagacccac tgtcctcaaa
     3721 ctcacagata cacctgcctc tgtctcccaa tctttgtgat taacggtgtg acctttaaac
     3781 accttaaaag acactgggct taagtgttat tttacataca atacttacct tacttcttac
     3841 aagccacctc ttactgataa cacttttttt atctacagaa acacctactt caagcatgat
     3901 ggcgtttcat tttattacat tggttctttt ctgtcattca caataaaatt tctcacgtgt
     3961 cttc
//
