LOCUS       NM_001411593            1911 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus eva-1 homolog A, regulator of programmed cell death
            (Eva1a), transcript variant 3, mRNA.
ACCESSION   NM_001411593 XM_030255337
VERSION     NM_001411593.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Li,L., Krafft,P.R., Zeng,N., Duan,R., Qi,X., Shao,A., Xue,F. and
            Zhang,J.H.
  TITLE     Microglia Autophagy Mediated by TMEM166 Promotes Ischemic Stroke
            Secondary to Carotid Artery Stenosis
  JOURNAL   Aging Dis 15 (3), 1416-1431 (2024)
   PUBMED   37611898
  REMARK    GeneRIF: Microglia Autophagy Mediated by TMEM166 Promotes Ischemic
            Stroke Secondary to Carotid Artery Stenosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1911)
  AUTHORS   Canham,L., Sendac,S., Diagbouga,M.R., Wolodimeroff,E., Pirri,D.,
            Tardajos Ayllon,B., Feng,S., Souilhol,C., Chico,T.J.A., Evans,P.C.
            and Serbanovic-Canic,J.
  TITLE     EVA1A (Eva-1 Homolog A) Promotes Endothelial Apoptosis and
            Inflammatory Activation Under Disturbed Flow Via Regulation of
            Autophagy
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (4), 547-561 (2023)
   PUBMED   36794585
  REMARK    GeneRIF: EVA1A (Eva-1 Homolog A) Promotes Endothelial Apoptosis and
            Inflammatory Activation Under Disturbed Flow Via Regulation of
            Autophagy.
            Erratum:[Arterioscler Thromb Vasc Biol. 2023 Jun;43(6):e230. doi:
            10.1161/ATV.0000000000000163.. PMID: 37224275]
REFERENCE   4  (bases 1 to 1911)
  AUTHORS   Li,J., Chen,Y., Gao,J., Chen,Y., Zhou,C., Lin,X., Liu,C., Zhao,M.,
            Xu,Y., Ji,L., Jiang,Z., Pan,B. and Zheng,L.
  TITLE     Eva1a ameliorates atherosclerosis by promoting
            re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1b
  JOURNAL   Cardiovasc Res 117 (2), 450-461 (2021)
   PUBMED   31977009
  REMARK    GeneRIF: Eva1a ameliorates atherosclerosis by promoting
            re-endothelialization of injured arteries via Rac1/Cdc42/Arpc1b.
REFERENCE   5  (bases 1 to 1911)
  AUTHORS   Martynova,E., Zhao,Y., Xie,Q., Zheng,D. and Cvekl,A.
  TITLE     Transcriptomic analysis and novel insights into lens fibre cell
            differentiation regulated by Gata3
  JOURNAL   Open Biol 9 (12), 190220 (2019)
   PUBMED   31847788
REFERENCE   6  (bases 1 to 1911)
  AUTHORS   Zhong,L., Zhou,J., Chen,X., Liu,J., Liu,Z., Chen,Y. and Bai,Y.
  TITLE     Quantitative proteomics reveals EVA1A-related proteins involved in
            neuronal differentiation
  JOURNAL   Proteomics 17 (5) (2017)
   PUBMED   28044434
  REMARK    GeneRIF: ATP synthesis, oxidative phosphorylation, and the TCA
            cycle, are involved in the EVA1A regulatory network.EVA1A plays an
            important role in neuronal differentiation.
REFERENCE   7  (bases 1 to 1911)
  AUTHORS   Zhang,S., Lin,X., Li,G., Shen,X., Niu,D., Lu,G., Fu,X., Chen,Y.,
            Cui,M. and Bai,Y.
  TITLE     Knockout of Eva1a leads to rapid development of heart failure by
            impairing autophagy
  JOURNAL   Cell Death Dis 8 (2), e2586 (2017)
   PUBMED   28151473
  REMARK    GeneRIF: Eva1a improves cardiac function.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1911)
  AUTHORS   Li,M., Lu,G., Hu,J., Shen,X., Ju,J., Gao,Y., Qu,L., Xia,Y., Chen,Y.
            and Bai,Y.
  TITLE     EVA1A/TMEM166 Regulates Embryonic Neurogenesis by Autophagy
  JOURNAL   Stem Cell Reports 6 (3), 396-410 (2016)
   PUBMED   26905199
  REMARK    GeneRIF: EVA1A deletion results in defective self-renewal and
            differentiation of NSCs.  EVA1A modulates autophagy through the
            PIK3CA/AKT-mTOR pathway.
REFERENCE   9  (bases 1 to 1911)
  AUTHORS   Shalom-Barak,T., Zhang,X., Chu,T., Timothy Schaiff,W., Reddy,J.K.,
            Xu,J., Sadovsky,Y. and Barak,Y.
  TITLE     Placental PPARgamma regulates spatiotemporally diverse genes and a
            unique metabolic network
  JOURNAL   Dev Biol 372 (1), 143-155 (2012)
   PUBMED   22967998
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC171502.2.
            
            On Aug 29, 2022 this sequence version replaced XM_030255337.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.609627.1,
                                           ERR3363658.3594529.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-224               AC171502.2         81040-81263
            225-418             AC171502.2         83677-83870
            419-606             AC171502.2         110417-110604
            607-1911            AC171502.2         131156-132460
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 35.81 cM"
     gene            1..1911
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /note="eva-1 homolog A, regulator of programmed cell
                     death"
                     /db_xref="GeneID:232146"
                     /db_xref="MGI:MGI:2385247"
     exon            1..224
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /inference="alignment:Splign:2.1.0"
     exon            225..418
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /inference="alignment:Splign:2.1.0"
     exon            419..606
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /inference="alignment:Splign:2.1.0"
     CDS             507..977
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /note="transmembrane protein 166; protein FAM176A; family
                     with sequence similarity 176, member A; eva-1 homolog A"
                     /codon_start=1
                     /product="protein eva-1 homolog A"
                     /protein_id="NP_001398522.1"
                     /db_xref="GeneID:232146"
                     /db_xref="MGI:MGI:2385247"
                     /translation="MKLPLSPSTEPVATEPLGMALLSSILAAWSYISENPERAALYFV
                     SGVCIGLFLTLAALVMRISCHTDCRRGPRRRCLQDRECSDSSDSEDGSEDTASDLSVR
                     RHRRFERTLNKNVFTSAEELERAQRLEERERIIREIWMNGQPEVPGTRSLNRYY"
     exon            607..1911
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1889..1894
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /note="hexamer: AATAAA"
     polyA_site      1911
                     /gene="Eva1a"
                     /gene_synonym="Fam176a; Tmem166"
                     /note="major polyA site"
ORIGIN      
        1 aggctcctct agagtgtgca gggctggagg agtccagagg ctgggtgtgc gcgttccgtg
       61 ctcagccctg attggggagg gccgccccaa attgctctcc ggcgcgcggg tgccccagcc
      121 acagggcgca gcagcacact gccgtgccct tccctaggtg atctgatcca ctccaggccc
      181 ccctgctcgg tccccggaag tacgcgccag tcgtcctaga gaagaccgag tgcctgcccc
      241 gtgccatgct cactccatga attttgactc gctcgatatt gtatcatcac tacgaagttc
      301 attgcagaag cacagagagg ctgcaacatt tgcctcacat cacacagctc attagtgctg
      361 gggccaacat tcacataaac ctttggattt cagaacacac actcttcttg ctacaaagga
      421 gagagggagg ggctgcactg agcctgaaga gagagagggg ctgcactgag cctgaagaca
      481 tatccaggag ctcttctcca tgccacatga agctgcccct cagccccagc acggaaccag
      541 tggccacgga gcccttggga atggctctgc tcagcagcat cctggcagcc tggtcttata
      601 tctcagaaaa ccctgagcga gccgctctgt actttgtctc tggagtgtgc attggcctgt
      661 tcctgacctt ggccgccttg gtgatgagga tctcctgcca cacggactgc cggcgaggtc
      721 cccggaggag gtgcttgcag gaccgcgagt gcagcgacag cagcgacagc gaggatggta
      781 gcgaggacac agcctctgac ctctcggtgc gcagacaccg ccgcttcgag aggactttga
      841 ataagaacgt gttcacctct gcagaggagc tggagcgcgc acagcgcttg gaggagcgcg
      901 aacgaatcat cagggagatc tggatgaacg ggcagcctga ggtgccgggc accaggagcc
      961 tgaaccgata ctactagggc tcagtgtgca ccccagaacc tctgcagatc gccgggatga
     1021 gagtgggttc tgcaggcaag gacgatgtgc aagagctgaa accagctctg ttaatgtggc
     1081 tggagagtgc atagcgccag ccagcctgtt tccccaggag aatggggatt tgctttagat
     1141 ccttgcctct ggagaaatag acaacccagg cagtggcatc tactctttca ggtcttgaca
     1201 gccacctggg cacaccagcc caatgctttc aggccttgga aaggtgctga ggaattcctc
     1261 tccaaccatg tgacagcgat gggacaagct gaaagtcctt ccgaagcgct cagaagtttg
     1321 ggcagactta tcctgtgcta ttattccttt ataaaaggaa aaaaaaatct catagctgga
     1381 gagctggggc atcagggtga ccgtaaccca gaaaatgtaa ttttctaaag gactccgtga
     1441 agaggaaatg gttttgattg agatagcatg atccatgcta attatttaaa gctaattttt
     1501 aaaacagaaa aaaaatcttg acaacctctg cattttaact gacacctcag ggattaaaat
     1561 cctatctttt agtctaggtc ccacctcatt catgaagatg aatagaactt actgtattat
     1621 gagatgtatt ggagacttag gatacatcaa taacctcaga ggataaaggg ttttattttt
     1681 tagtactcta taaactatgt attgacatgt gtatttgaaa atgctgcata aaaatagaaa
     1741 ccgtgtgtcc tttccttttg gagagaaaag tctcatgact tcagagactc atgatattta
     1801 aataatattt tgtttaaaaa aatagtttgg atttccaaga atggaagcca gaggtgagaa
     1861 ttaaatccaa aattagaatg gtatcgaaaa taaacattcc atgaaaatct t
//
