LOCUS       NM_001252095            1688 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus electron transfer flavoprotein beta subunit lysine
            methyltransferase (Etfbkmt), transcript variant 2, mRNA.
ACCESSION   NM_001252095
VERSION     NM_001252095.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Shimazu,T., Furuse,T., Balan,S., Yamada,I., Okuno,S., Iwanari,H.,
            Suzuki,T., Hamakubo,T., Dohmae,N., Yoshikawa,T., Wakana,S. and
            Shinkai,Y.
  TITLE     Role of METTL20 in regulating beta-oxidation and heat production in
            mice under fasting or ketogenic conditions
  JOURNAL   Sci Rep 8 (1), 1179 (2018)
   PUBMED   29352221
  REMARK    GeneRIF: These results further indicate that METTL20-mediated
            protein methylation is not essential in humans but more likely
            modulates energy production during fasting or under other metabolic
            conditions where beta-oxidation is up-regulated.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1688)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 1688)
  AUTHORS   Rhein,V.F., Carroll,J., He,J., Ding,S., Fearnley,I.M. and
            Walker,J.E.
  TITLE     Human METTL20 methylates lysine residues adjacent to the
            recognition loop of the electron transfer flavoprotein in
            mitochondria
  JOURNAL   J Biol Chem 289 (35), 24640-24651 (2014)
   PUBMED   25023281
REFERENCE   5  (bases 1 to 1688)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY081844.1, BY135669.1 and BC048711.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1, and encodes the longer isoform (a). Variants 1-4 all
            encode isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.227367.1,
                                           SRR5189682.121364.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                BY081844.1         1-51
            52-403              BY135669.1         1-352
            404-1688            BC048711.1         186-1470
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 78.8 cM"
     gene            1..1688
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /note="electron transfer flavoprotein beta subunit lysine
                     methyltransferase"
                     /db_xref="GeneID:320204"
                     /db_xref="MGI:MGI:2443575"
     exon            1..192
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /inference="alignment:Splign:2.1.0"
     exon            193..302
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /inference="alignment:Splign:2.1.0"
     exon            303..711
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /inference="alignment:Splign:2.1.0"
     CDS             419..1186
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /note="isoform a is encoded by transcript variant 2;
                     methyltransferase-like protein 20; protein N-lysine
                     methyltransferase METTL20; ETFB lysine methyltransferase;
                     methyltransferase like 20"
                     /codon_start=1
                     /product="electron transfer flavoprotein beta subunit
                     lysine methyltransferase isoform a"
                     /protein_id="NP_001239024.1"
                     /db_xref="CCDS:CCDS20715.1"
                     /db_xref="GeneID:320204"
                     /db_xref="MGI:MGI:2443575"
                     /translation="MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYL
                     EENTEVTSSGSLTPEIQLRLLTPRCKFWWERADLWPYSDPYWAIYWPGGQALSRYLLD
                     NPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDIDPIAGMAITLNCKLNGLNPFP
                     VLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIGDPGRPQFS
                     GHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP"
     exon            712..842
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1672
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1648..1653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /note="hexamer: AATAAA"
     polyA_site      1672
                     /gene="Etfbkmt"
                     /gene_synonym="4833442J19Rik; ETFB-KMT; Mettl20"
                     /note="major polyA site"
ORIGIN      
        1 ttcggtccgg gcggccccgc gttccgcgtc tcattggttc caaatttcgc cgccttcacg
       61 tacccactac gctgtgtagt tagagccacg ggctcagtct tcctctcccg aaaaaggaga
      121 cctaccgtac ttgttttttt ttttctcctg cacgtgaagg gcgagttcca cttgctgatc
      181 gaataaagtc aggttttgat atttctccac tgctacctgt tgagctcaga agtttttaaa
      241 ctcttggcag agaaaactga atccaatcaa gaacctttag acagtaatgc cagttcaatc
      301 agagatccag aaattccagt gtcaattaag tggaaagatg gaccctcatt ctatctgctt
      361 gggaaacagt ccttattaat agcaacactg acagaacctg aggtttggag atgtactaat
      421 ggctttcagc ctgtgttgga aagctcccag gagcccatgg agtttcctgc aggctgtgaa
      481 caatgggtct cccctgtttc tctggaggac agtggggagc tgtttggatc ctaagatgaa
      541 agcctacctg gaagagaaca ctgaagtcac cagcagtggt agccttaccc cggaaatcca
      601 gttacggctt ttaaccccca ggtgcaagtt ttggtgggaa agggctgacc tgtggcccta
      661 cagtgatccc tactgggcta tttactggcc aggaggccag gctctgtcta ggtatctttt
      721 ggataaccct gctgttgtta gaggaaagtc tgtcttagat cttgggagtg gatgtggagc
      781 tacagccatt gctgccaaga tgagtggagc atcaaagatc ttggccaacg acatagaccc
      841 cattgcagga atggcgatta cactaaattg caaattaaat ggactgaatc cttttcctgt
      901 tttaactaaa aacattttga atacccaaca aggtaagttt gatctcattg ttcttggaga
      961 tatgttttat gatgaagacc ttgcagacag tcttcatctg tggctgcaga actacttttg
     1021 gacccatgga acccgagtac tgattggtga ccctgggagg ccccagttca gtggacatag
     1081 cattcggcat caactgtacc aactagtaga atacacactt ccagagccaa ctcagcaaga
     1141 gaacaatgga ctgaccacaa gtgctgtatg ggattttcat ccctgatttg ttaaagtgct
     1201 ttcaagtatg aatatagttc tgtttgtgtt aagttctaaa aatatcctct gtaggaggta
     1261 ttcaacagtt taaagaatgc aattcagtgg cttgtggtgg attgggcctt gaatactacc
     1321 acttgggagg ctgaagtagg cgcatctctg tgactttgag gacagcttgg tctatagtga
     1381 gttcaaggac agccagagct atatagtaag accctgttcc aaaaatagat agatagataa
     1441 taaagtaaat ttcttcattc ctttgctgtg acaccttgaa taactgtatg gtttattaaa
     1501 gttagttatg gttatgttcc catttcacat accaaggtgt caggatttta taacaaagca
     1561 ctctatcata tacaattgca tatgtagaag gtatatttat atatgtatgt atgtatggat
     1621 gtaatatata taatttgctt cagtattaat aaaatatctc tggaaagata aaaaaaaaaa
     1681 aaaaaaaa
//
