LOCUS       NM_026576               4188 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus Ewing tumor-associated antigen 1 (Etaa1), transcript
            variant 1, mRNA.
ACCESSION   NM_026576
VERSION     NM_026576.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Miosge,L.A., Sontani,Y., Chuah,A., Horikawa,K., Russell,T.A.,
            Mei,Y., Wagle,M.V., Howard,D.R., Enders,A., Tscharke,D.C.,
            Goodnow,C.C. and Parish,I.A.
  TITLE     Systems-guided forward genetic screen reveals a critical role of
            the replication stress response protein ETAA1 in T cell clonal
            expansion
  JOURNAL   Proc Natl Acad Sci U S A 114 (26), E5216-E5225 (2017)
   PUBMED   28607084
  REMARK    GeneRIF: ETAA1 deficiency decreased clonal expansion cell
            autonomously within the responding T cells, causing no decrease in
            their division rate but increasing TP53-induced mRNAs and
            phosphorylation of H2AX, a marker of DNA replication stress induced
            by the ATM and ATR kinases.
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK019965.1, AL627070.16 and AK018594.1.
            
            On May 4, 2018 this sequence version replaced NM_026576.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK019965.1, SRR1660813.113038.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164142
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-666               AK019965.1         2-667
            667-667             AL627070.16        69793-69793
            668-2636            AK019965.1         669-2637
            2637-2859           AK018594.1         1934-2156
            2860-4188           AL627070.16        77242-78570
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 10.44 cM"
     gene            1..4188
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /note="Ewing tumor-associated antigen 1"
                     /db_xref="GeneID:68145"
                     /db_xref="MGI:MGI:1915395"
     exon            1..313
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..2715
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ewing's tumor-associated antigen 1 homolog"
                     /codon_start=1
                     /product="ewing's tumor-associated antigen 1 homolog
                     isoform 1"
                     /protein_id="NP_080852.2"
                     /db_xref="CCDS:CCDS24452.1"
                     /db_xref="GeneID:68145"
                     /db_xref="MGI:MGI:1915395"
                     /translation="MQLKDGGTGMSRRRKHADSPARRSTPHRAAAKNCRPAAEPWLRE
                     SRAACSSQLRRAGTRSARRAQRQAAADGGRSPRGKETPVQIVKMDLLSCTFSSPNDPD
                     GQTDIFWDQNSPMTKQLGKGRKKQISSAYSDEISHIVNRIAPQDEKPVTNSMLGVWIG
                     ETAIPCTPGVAKEKSRVKASCTKLKTKNREKELMKLAQQFDKNMEELDVIQEQDGKNH
                     DFIQMTSKMGHLDNHKDSVQKPSGDVVPEITCTPVKKQMKGDSRISLAKAQDSSQKPF
                     DQNVEAAFNAIFDGSTQMCSGQLSQDLLDAFLNNSKTSLRKKNALLQEEIITTETLLT
                     ENLLNKTPISLSPQIDTTVILNSCVTPCPKTPAAPDTQLDELTANDFEDDWESLLGSE
                     PFLMENAEMLEFVPSTTAQDTCQKAICTSVGENDTITSRTNMNLGGRLRDSKVTLDLP
                     SKTRNGELRNAGEHRFSSHPGDESRKVPFTGNKVSFEKSVTSIVSKDEDYVAVSNLEK
                     VKEDSRNKCILNKHSSNKSSSYTRYPSKQSSELGVNLPLQVPTTDPFDSVFLGKENIV
                     CSTNQSHGSKLNSSFDDWNDPLLASEMVEACHRLEATWDAGEVDDDLFCQACDDIERL
                     TQQENKGSEESESVSYTSTRGSRSSSTASKQASQSAPSKHWNVVSSAVPLSLANKSQM
                     SKPVTVQKRGRCGDGPNILDATNLSVCSKNSSDNKRGPVQVNSSKFVLGGSSNLNVNL
                     GLMSTKIATNMKLSTQQLSHNSLADTAQNDNKILKLPKFTFKKKNPQLNQNHLVGSVP
                     VGKISEDLGKRETVNSLLEANQQQSSINYSESLKPSSPDEEERNRKYSPEEIQRKRQE
                     ALVRRKAKALHTVQSAPISLP"
     exon            314..439
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            440..516
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            517..629
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            630..2590
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2591..4188
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3028..3033
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3052
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /note="major polyA site"
     regulatory      4163..4168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4188
                     /gene="Etaa1"
                     /gene_synonym="5730466H23Rik"
ORIGIN      
        1 cgacttgcaa agatggcgct gcacggtggc gcgcgggctc tctgtcgagc agttgccctg
       61 ctccgctgta gcaaaatgtg aatgcagctg aaggacggtg gcacgggaat gagtcggcga
      121 aggaagcatg cggacagccc tgcccggagg agcacgccgc acagggcggc tgctaagaac
      181 tgccgcccgg cggcagagcc gtggctgcgg gagtccaggg cggcctgcag ctcccagctc
      241 cgccgggcgg gaacgcggtc tgcgcggcgg gcgcagcggc aggcagcggc tgacggcggg
      301 cgcagtcccc ggggaaagga gacgccagta caaatcgtga aaatggattt attatcctgt
      361 accttcagtt ctccaaatga tccagatgga caaactgata tcttctggga tcaaaattct
      421 ccaatgacaa agcagttagg taaaggaaga aaaaagcaga tttccagtgc atacagtgat
      481 gagatttcac atattgttaa ccgtattgct cctcaggacg agaaaccagt gacaaattcc
      541 atgctgggag tatggattgg tgaaactgct attccttgta ctcctggcgt agcaaaagag
      601 aagtcaagag tgaaagccag ctgcacaaaa ttaaaaacaa aaaatcgaga aaaagaactt
      661 atgaaattgg cccaacagtt tgataaaaat atggaagaac ttgatgtgat ccaagagcaa
      721 gatgggaaga atcatgattt tatccagatg acttcaaaaa tgggacattt agataaccat
      781 aaagattctg tgcagaagcc gtcaggtgat gtagttcctg aaatcacttg tactcctgtg
      841 aagaagcaaa tgaaaggaga cagtagaata tccttggcaa aggcacaaga cagcagtcag
      901 aaaccctttg accaaaatgt tgaagcagcc tttaatgcta tatttgatgg ttccactcag
      961 atgtgtagtg gacagctaag ccaagatctg ttagatgctt ttttgaacaa tagtaaaact
     1021 tccttgagaa agaaaaatgc cttactacaa gaggaaataa ttactactga aactttgctc
     1081 actgaaaacc tgctaaataa aactccaata tcactttccc ctcaaataga tactaccgta
     1141 atactaaact catgtgtgac tccctgtcct aagacaccag cagcacctga tacacagctt
     1201 gatgagttga ccgctaatga ttttgaggat gattgggaaa gcttactagg cagtgaaccc
     1261 ttccttatgg aaaatgctga aatgcttgaa ttcgttcctt ctacaactgc ccaagatact
     1321 tgtcagaagg caatttgtac ctcagttggt gaaaatgata caatcacatc aagaacaaat
     1381 atgaatctag gtggaaggtt aagagattca aaagtaacac tggatcttcc ctcaaagaca
     1441 cgaaacggag agctaagaaa tgctggagaa cataggtttt catcacatcc aggtgatgaa
     1501 tcaaggaagg taccattcac aggaaataaa gtgagttttg agaaatctgt cactagcata
     1561 gttagtaaag atgaagatta tgttgctgtc tctaatcttg aaaaagtgaa agaagatagt
     1621 cgcaataagt gcatacttaa taaacattcc tctaacaagt ctagttcgta cacaaggtat
     1681 cctagtaaac aaagttccga gcttggtgtt aatctgcctt tgcaagtacc cactactgat
     1741 ccatttgatt ctgtgttctt gggcaaagaa aatattgtgt gtagcacaaa tcagtctcat
     1801 ggatcaaagt tgaattcttc ctttgacgac tggaatgatc cgctgttggc cagtgaaatg
     1861 gttgaagcat gccatcggtt agaggctacc tgggacgcag gcgaggtaga tgatgatttg
     1921 ttctgtcagg cttgtgatga cattgaaaga ctaactcagc aagaaaacaa gggcagcgag
     1981 gagtcagaga gtgtaagtta tacttccact cgtgggtcca gaagtagctc tactgcatct
     2041 aaacaagcaa gccagtcggc gccatctaaa cattggaatg ttgtcagctc tgcagtgcca
     2101 ttgtctttag caaataaatc acagatgagt aagccagtga cagtacagaa aagaggcaga
     2161 tgtggagatg ggcccaatat tttggatgct acaaatttgt ctgtgtgttc taagaattca
     2221 agtgataata agcgtggacc agtacaagtg aatagctcaa aatttgttct tggaggaagt
     2281 tcaaatttga atgtaaattt aggtcttatg agcacaaaaa ttgctactaa tatgaagttg
     2341 agtactcagc agctatccca caacagctta gcagatacag ctcagaatga caacaaaata
     2401 ctgaagttac caaaattcac atttaagaag aaaaatcctc agttaaatca aaatcatcta
     2461 gtgggaagtg tgcctgttgg caaaatctca gaggatttag gaaaaaggga aactgtcaat
     2521 tcattgcttg aagctaatca gcagcaatct tcaataaact attctgagtc tttgaaacca
     2581 tcttccccag atgaagaaga gagaaatagg aagtattctc ctgaagaaat tcaacgaaaa
     2641 aggcaggaag cactggttcg aagaaaggcc aaagctttgc atactgtgca gtcagctccc
     2701 atttcactgc cttgatgaaa gtcgagttag atggtttcac aactattaat aactatctgt
     2761 gataggactt ttgttctgat ttctctgtga gaaatgtaat actgacttat aaagcctgag
     2821 cttctacttt attatttaat aaatcaatgt ttacagtggt aaatgaaacc ttttcagaga
     2881 tgtatgtgaa ttatgtatat aagtttttga aaagttagca gttatgtata tacagaggaa
     2941 agtacttgtc tttattaaat ggtagcagat gagcatcgaa aatagctata aagcttctgt
     3001 cgaacgaagg ctttctctag cacaaagatt aaaattgcta agtaaacaac aacttggttt
     3061 tttgtgtcat tatcagttaa ggttttttat acattgtcaa tttttaacta atattcccat
     3121 agtctaactg gtcttgctga aaagtagaga atttcttttt attttcaaaa aaattgtgtt
     3181 taattgctgc ccagaagtag ctaactttaa tatttctaac atgtgattca ataggaaaat
     3241 atttcactgt taggaaaccc acagactagg cattaagtct agtttagcaa aaagaaaaaa
     3301 aaagctttat tatgtaagta ataaagcatc gttgacattt agaattggta gaaacatcgc
     3361 ctctggtcga gcttctctat catataggtt agacagaaca attcaagctg ttctttttct
     3421 ttgtgtacat gtgtgtttga gtcagggtct tgctctgtgg cccagactgg ccttcaactc
     3481 tctctgttac ccaaatggcc ccacactatc agtgatcctc ttgcctcagc ctccctgtgc
     3541 ttttaagtac agatgtgtgc caccatgccc aacttactgt gctactttca gagtcacaga
     3601 caaggtcagc ctgtccttgt gtgagacaat cttaactgtt gcatgacttg cttcagtctg
     3661 ttcgtactta aaataactgg aaggcacaga aacactttaa agtacattaa aggctgcttg
     3721 ttagcagtac ttacatacat tctttatctt aaagaactaa ctaatctttc caggattcaa
     3781 aattagactt tctattctca cttttcttat cactctaatt tcccttctaa ttaattattt
     3841 aacttaagtg tttttccctc tgtcatcccc ccctccctcc ccttttggct tttaaacaat
     3901 gagccagtaa taaagccttt aaactaaagg gggcgggggt ggcagtaagg atgctagtag
     3961 ttttttaaat gctttttaaa taatatgaag aaaattatag atatgaagta ttcattttga
     4021 tctgatgttc actactgttc aggtgtctta gagctttgac aaactgatag ttatgtatca
     4081 tttttcaaat gttattttta tatatacagt aaaacagatt ttagtttccc tgtattatca
     4141 aagagtatat tataaagacc taaataaatg ttttaagtta ttatgtaa
//
