LOCUS       NM_177775               4491 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus extended synaptotagmin-like protein 3 (Esyt3), mRNA.
ACCESSION   NM_177775
VERSION     NM_177775.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Ma,Y., Zhao,R., Guo,H., Tong,Q., Langdon,W.Y., Liu,W., Zhang,J. and
            Zhang,J.
  TITLE     Cytosolic LPS-induced caspase-11 oligomerization and activation is
            regulated by extended synaptotagmin 1
  JOURNAL   Cell Rep 42 (7), 112726 (2023)
   PUBMED   37393619
REFERENCE   3  (bases 1 to 4491)
  AUTHORS   Zhang,Y., Guan,Y., Pan,S., Yan,L., Wang,P., Chen,Z., Shen,Q.,
            Zhao,F., Zhang,X., Li,J., Li,J., Cai,D. and Zhang,G.
  TITLE     Hypothalamic extended synaptotagmin-3 contributes to the
            development of dietary obesity and related metabolic disorders
  JOURNAL   Proc Natl Acad Sci U S A 117 (33), 20149-20158 (2020)
   PUBMED   32747560
  REMARK    GeneRIF: Hypothalamic extended synaptotagmin-3 contributes to the
            development of dietary obesity and related metabolic disorders.
REFERENCE   4  (bases 1 to 4491)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 4491)
  AUTHORS   Tremblay,M.G. and Moss,T.
  TITLE     Loss of all 3 Extended Synaptotagmins does not affect normal mouse
            development, viability or fertility
  JOURNAL   Cell Cycle 15 (17), 2360-2366 (2016)
   PUBMED   27399837
REFERENCE   6  (bases 1 to 4491)
  AUTHORS   Sclip,A., Bacaj,T., Giam,L.R. and Sudhof,T.C.
  TITLE     Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and
            Fertile without Obvious Endoplasmic Reticulum Dysfunction
  JOURNAL   PLoS One 11 (6), e0158295 (2016)
   PUBMED   27348751
  REMARK    Erratum:[PLoS One. 2024 Feb 6;19(2):e0298645. doi:
            10.1371/journal.pone.0298645.. PMID: 38319918]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4491)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 4491)
  AUTHORS   Herdman,C., Tremblay,M.G., Mishra,P.K. and Moss,T.
  TITLE     Loss of Extended Synaptotagmins ESyt2 and ESyt3 does not affect
            mouse development or viability, but in vitro cell migration and
            survival under stress are affected
  JOURNAL   Cell Cycle 13 (16), 2616-2625 (2014)
   PUBMED   25486202
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK086367.1, AK142948.1 and BY754935.1.
            
            On Dec 15, 2006 this sequence version replaced NM_177775.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK086367.1, AK220532.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                AK086367.1         2-80
            80-316              AK142948.1         108-344
            317-2599            AK086367.1         318-2600
            2600-2643           AK086367.1         2602-2645
            2644-4315           AK086367.1         2647-4318
            4316-4491           BY754935.1         500-675
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 51.54 cM"
     gene            1..4491
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /note="extended synaptotagmin-like protein 3"
                     /db_xref="GeneID:272636"
                     /db_xref="MGI:MGI:1098699"
     exon            1..480
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..2817
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /note="E-Syt3; family with sequence similarity 62 (C2
                     domain containing), member C; family with sequence
                     similarity 62, member C"
                     /codon_start=1
                     /product="extended synaptotagmin-3"
                     /protein_id="NP_808443.2"
                     /db_xref="CCDS:CCDS40739.1"
                     /db_xref="GeneID:272636"
                     /db_xref="MGI:MGI:1098699"
                     /translation="MQPEEPCAPSAPGGPDVPERGQRSRDPGPRLSGQLLPELYSFVA
                     RVLFYLAPVYLAGYLGLSVTWLLLGALLWMWWRRNRRGKLGRLEAAFEFLEHEREFIS
                     RELRGQHLPAWIHFPDVERVEWANKIIIQIWPYLSMIMENKIREKLEPKIREKSIHLR
                     TFTFTKLYFGQKCPKVNGVKVHTDKRNRRKVTLDLQICYIGDCEISVELQKIRGGVSG
                     VQLQGTLRVILEPLLVDKPFIGAVTVFFLQKPHLQINWTGLTNLLDMPGINELSDSLL
                     EDLIAAHLVLPNRVTVPVKKGLDVTNLRVPLPCGVIRVHLLEAKKLAQKDNFLGLGGK
                     SDPYAKVSIGLQHCRSRTIYKNLNPTWNEVFEFMVYEVPGQDLEVDLYDEDTDKDDFL
                     GSLQICLGDVMKNRVVDEWFALNDTTSGRLHLRLEWLSLLTDQEALTENDSGLSTAIL
                     VVFLENACNLPRNPFDYLNGEYRAKKLSRFVKNKASRDPSSYVKLTVGKKTFTSKTCP
                     HSKDPVWSQVFSFFVHSVAAEQLCLKVLDDELECALGVLEFPLCRILPCADLTLEQCF
                     QLDHSGLDSLISMRLVLRFLRVEGRELGSPYTGPDALKKGPLFIKKVATNQGCKAPPL
                     NEGLADVTSTSNPASYIKGASKSIDNISAATTDPEPMPEPQGPGPEPKGKDSARGLCE
                     SPGKKKNPATTFLTVPGLHSPGPIKSPRPMSRPAFPFAWPLTRVAPSMSSLNSLASSC
                     FDLTDVSLNTEAGDSRQGRLGEIQLTVRYVCLRHCLRVLVNGCRNLTPCTSSGADPYV
                     RIYLLPERRWASRKKTSVKQKTLEPLFDETFEFFVPMGEVQKRSLDVAVKNSRPLGSH
                     RRKELGKVLIDLSKQDLIKGFSQWYELTADGQPRS"
     exon            481..522
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            523..657
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            658..734
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            735..801
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            802..891
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            892..947
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1068
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1239
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1322
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1371
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1461
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1524
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1587
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1656
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1743
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1893
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2393
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2492
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2624
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2730
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            2731..2780
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..4491
                     /gene="Esyt3"
                     /gene_synonym="D930024E11; D9Ertd280e; Fam62c; mKIAA4186"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagcttgga gaggctggcg gagcaggagc tgcgcggctg cggagtcctc gtgccttgag
       61 gacagatgtg cgccggcata gggggtgcac caacctgagc tggggtgaag cttctagaga
      121 ggacaagccg gcgacagcga gatgcagccg gaggagccct gcgccccgag cgcgcccggc
      181 ggtcctgacg tcccggagag aggccagcgc tcgcgggacc ccggaccgcg cctgtcgggc
      241 cagctactgc ccgagctcta cagcttcgtg gcgcgcgtcc ttttctacct ggcaccggtc
      301 tacctggcgg gctacctggg gctcagcgtc acctggctgc tgcttggcgc cctgctctgg
      361 atgtggtggc gccggaaccg ccgcgggaag ctggggcgct tggaggccgc cttcgaattc
      421 ctggagcacg agcgcgagtt catcagccgt gagctgcggg gccagcattt gccagcctgg
      481 atacatttcc cagatgtgga gcgtgtggag tgggccaata agatcatcat acagatctgg
      541 ccctacctaa gcatgatcat ggagaacaag atccgggaga agctggagcc caagatccgg
      601 gagaagagca tccacctcag aaccttcacc ttcaccaagc tctactttgg gcagaagtgt
      661 cccaaggtga acggcgtcaa ggtacacacg gacaaacgca accggaggaa agtgaccctg
      721 gacctgcaga tctgctacat cggggactgt gagatcagcg tggaacttca gaagatccgg
      781 ggcggtgtga gcggcgtcca gttgcagggg accctgcgtg tcattctgga gcccctgctg
      841 gtggacaagc ccttcatagg agctgtgaca gtgttcttcc ttcaaaagcc gcacctgcag
      901 attaactgga caggcttgac caacctgctg gacatgccgg ggatcaatga gttatcagac
      961 agcctgctgg aggacctcat tgctgcccac ctggtgctgc ccaaccgtgt gactgtgcct
     1021 gtgaagaagg ggctcgatgt aaccaacctg cgcgtccctc tcccctgtgg tgtgatccga
     1081 gtccatttgc tggaggccaa gaagctggcc cagaaggaca acttcctagg gctcggaggc
     1141 aagtcagacc cctacgccaa ggtgagcatt ggcttgcagc attgccggag cagaaccatc
     1201 tacaagaacc tgaaccccac ctggaatgaa gtatttgagt ttatggtgta cgaggtacct
     1261 gggcaggacc tggaggtgga cctgtatgat gaggatactg acaaggatga cttcctgggc
     1321 agcctgcaga tctgccttgg agatgtcatg aagaacagag tggtggatga gtggtttgcc
     1381 ctgaatgaca cgaccagtgg gcggctgcac ctgcggctgg agtggctctc actactcact
     1441 gaccaggaag cgctgacgga gaatgatagt ggcctctcca ctgccatcct cgtggtcttc
     1501 ttagagaatg cctgcaacct gccgagaaac ccttttgact atctgaatgg tgaatatcga
     1561 gccaaaaaac tctccaggtt tgtcaaaaat aaggccagca gagacccttc ttcatacgtc
     1621 aagctcactg taggcaagaa gacctttaca agtaagactt gtccccacag caaggaccct
     1681 gtgtggagcc aggtgttctc cttctttgtg cacagcgtgg cagccgagca gctctgcctg
     1741 aaggtgctgg acgatgagct agagtgtgct ctgggagtgc tggagttccc cctgtgccgg
     1801 atcctcccct gtgctgacct caccctcgaa cagtgcttcc agctggatca ctcaggcctg
     1861 gacagcctca tctccatgag gctggtgctt cggttcctac gtgtggaggg acgagagctg
     1921 ggaagcccat acactggacc cgacgctcta aagaaaggtc ctctgttcat caagaaggtg
     1981 gccaccaacc agggttgtaa agccccaccc ctaaacgaag gccttgcaga tgtgacaagc
     2041 acctcgaacc ctgcctctta catcaaagga gcctccaaga gcattgacaa tatcagcgca
     2101 gccaccacag accctgaacc tatgcctgag ccccaggggc caggcccgga gcccaaaggc
     2161 aaggacagtg ccagagggct ctgtgagtcc cctggaaaga agaagaaccc agctaccacc
     2221 ttcctgactg tcccaggtct ccactctcca gggcccatca agtcacccag acccatgagc
     2281 cgccctgcct tcccattcgc atggccactc acgagggtgg ctcccagcat gtcctcactc
     2341 aactccctgg cctcttcctg ctttgacctg acagatgtca gcctcaacac tgaagctggg
     2401 gattccaggc agggacggct gggtgagatt cagctcacag tgcgctatgt gtgtctccga
     2461 cactgtctca gagtgctggt caacggctgc agaaacttaa caccatgcac cagcagtgga
     2521 gctgacccct acgtccgcat ctacctgctg ccagagagaa gatgggcgag tcggaagaag
     2581 acctcagtaa aacagaagac cctggagccc ctattcgatg agacgtttga gttttttgtc
     2641 cccatgggag aagtgcagaa gagatcactg gatgtcgcag tgaaaaacag caggcctctc
     2701 ggctcacaca gaaggaagga gttggggaaa gtgctgattg acttatcaaa gcaagatctg
     2761 attaagggct tctcacagtg gtatgaactg actgcagatg gacagcccag aagctgacca
     2821 tgagctctga gctccagtgt tagaaagtgt atatgtgtat ttttgtcatt taaatcagaa
     2881 catccatttg atggaaatat acactgtatg aactcttaac tacatgatac tacatggcta
     2941 gtagaggaag gtcatgtaaa agcagggact gtttctgtta atttggatta attcaaatcc
     3001 acagagaaac tgtggtattt gtatctctaa tgaggctcca aaacctcaga ggtgactttt
     3061 cactttgttc acatcagtgc cctgtcccca gcacggggat gtgggggagg gagcctgttt
     3121 agagtaactc ctgggcagtt actgagcaca ggagagcact cttgtggaaa taaaaagcat
     3181 ccaaagtcaa agccacccag agctaaccct ggttgcttgt aagcctacat ttgccccagg
     3241 ttctgacgtg gagctgcagt gtattttcct aggggcaaca gtgtgaagag ttacatgctc
     3301 tgggaaagcc taggggacaa acctctggta tgtggcatta tttccatagg aaattttgag
     3361 cccagcccca aaccaaggtc tatgtctgaa ctctaggttc aggaggctgc aaaacagtta
     3421 attcctagga ggaaacggag cctttatatt ctgttaggca agggtgagac gaagtcctgc
     3481 tgttcgccca aagacatgtt aagaggaaaa tatgtacatt atgtatttaa acaagtgcct
     3541 tttacacatt tcagtttgta agaccagata gacttctgtt ctgtttctag ggtaagccac
     3601 ccattcagac aataatccct gctatgttcc tgttgtcttc aatgatgctt ttccttttta
     3661 tttattatta ttctgtgtag gatgtcacgt gtcacagcac acgtgggcat caaacacttt
     3721 ctgtaggttc tgggcaggct tgtgcaacag atgacctttg ccctctgagc cagccatccc
     3781 tgatgctctg taaagaatgc aacagagcgg ttatggcggc aaatgtcttt agtcccagcc
     3841 ctgggaggct gaggcaggta gtctctgtga gttcaggcca gacaacacta caatgtatga
     3901 ctgagtctca acaaaaatgg caatgttctg cctgggcctg gtcccagaac ttgggaggct
     3961 gaggctggcc tgccttaata acacaatagt gttcactctg acttgtaggt tatgtgttat
     4021 ttattataga ctgtaatatt catagttcca actcccctat caagtgtgag atgtggtttc
     4081 tgctactgta ttcttgcgca gatgtgtgtt cattccgcag catcctgagg acacagaaca
     4141 gtccagatac tgtgccgtcc tttccaatgg ccagtccata ctggaatggt ctcttcctgt
     4201 gtagcaccgt ccattttaac aattctcagt gtaggcaggt ggatctctga gtccgaggcc
     4261 agcctggtct acagaggaac ttctgggaca ggctacatag agaaaccctg tttccaaaac
     4321 aaaaacaaaa acaaaaaaca attcttagtg ttgtaacatt agttttttaa tgtttacaag
     4381 ctctgtactt ggttacatac taagcaacta cactactttg ataaatgttt tagactatat
     4441 cccaattgaa ccacagacta cttgatgcat aataaaagtt ttgtaaatat t
//
