LOCUS       NM_028731               5556 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus extended synaptotagmin-like protein 2 (Esyt2), mRNA.
ACCESSION   NM_028731 XM_619050
VERSION     NM_028731.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Maruta,T., Hidaka,K., Kouroki,S., Koshida,T., Kurogi,M., Kage,Y.,
            Mizuno,S., Shirasaka,T., Yanagita,T., Takahashi,S., Takeya,R. and
            Tsuneyoshi,I.
  TITLE     Selective optogenetic activation of NaV1.7-expressing afferents in
            NaV1.7-ChR2 mice induces nocifensive behavior without affecting
            responses to mechanical and thermal stimuli
  JOURNAL   PLoS One 17 (10), e0275751 (2022)
   PUBMED   36201719
  REMARK    GeneRIF: Selective optogenetic activation of NaV1.7-expressing
            afferents in NaV1.7-ChR2 mice induces nocifensive behavior without
            affecting responses to mechanical and thermal stimuli.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Jiang,D., Burger,C.A., Casasent,A.K., Albrecht,N.E., Li,F. and
            Samuel,M.A.
  TITLE     Spatiotemporal gene expression patterns reveal molecular
            relatedness between retinal laminae
  JOURNAL   J Comp Neurol 528 (5), 729-755 (2020)
   PUBMED   31609468
REFERENCE   3  (bases 1 to 5556)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 5556)
  AUTHORS   Tremblay,M.G. and Moss,T.
  TITLE     Loss of all 3 Extended Synaptotagmins does not affect normal mouse
            development, viability or fertility
  JOURNAL   Cell Cycle 15 (17), 2360-2366 (2016)
   PUBMED   27399837
REFERENCE   5  (bases 1 to 5556)
  AUTHORS   Sclip,A., Bacaj,T., Giam,L.R. and Sudhof,T.C.
  TITLE     Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and
            Fertile without Obvious Endoplasmic Reticulum Dysfunction
  JOURNAL   PLoS One 11 (6), e0158295 (2016)
   PUBMED   27348751
  REMARK    Erratum:[PLoS One. 2024 Feb 6;19(2):e0298645. doi:
            10.1371/journal.pone.0298645.. PMID: 38319918]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5556)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 5556)
  AUTHORS   Herdman,C., Tremblay,M.G., Mishra,P.K. and Moss,T.
  TITLE     Loss of Extended Synaptotagmins ESyt2 and ESyt3 does not affect
            mouse development or viability, but in vitro cell migration and
            survival under stress are affected
  JOURNAL   Cell Cycle 13 (16), 2616-2625 (2014)
   PUBMED   25486202
  REMARK    GeneRIF: Mouse with deletions are generated:esyt2(-/-)/esyt3(-/-)
            develop normally and are viable and fertile; esyt2(-/-)/esyt3(-/-)
            embryonic fibroblasts show reduced ability to migrate, are less
            resistant to stringent culture conditions and to oxidative stress.
REFERENCE   8  (bases 1 to 5556)
  AUTHORS   Abbott,S.B., Stornetta,R.L., Socolovsky,C.S., West,G.H. and
            Guyenet,P.G.
  TITLE     Photostimulation of channelrhodopsin-2 expressing ventrolateral
            medullary neurons increases sympathetic nerve activity and blood
            pressure in rats
  JOURNAL   J Physiol 587 (Pt 23), 5613-5631 (2009)
   PUBMED   19822543
  REMARK    GeneRIF: ChR2 expression had no discernable effect on the putative
            vasomotor neurons at rest
REFERENCE   9  (bases 1 to 5556)
  AUTHORS   Groer,G.J., Haslbeck,M., Roessle,M. and Gessner,A.
  TITLE     Structural characterization of soluble E-Syt2
  JOURNAL   FEBS Lett 582 (28), 3941-3947 (2008)
   PUBMED   18977228
  REMARK    GeneRIF: Murine E-Syt2 was studied by small-angle X-ray scattering
            (SAXS) providing a three-dimensional image of a protein with three
            C2 domains.
REFERENCE   10 (bases 1 to 5556)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044360.1, AK014813.1, BC059230.1 and AI838753.1.
            
            On Sep 22, 2005 this sequence version replaced NM_028731.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK157352.1, SRR1660819.169851.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-893               AK044360.1         2-894
            894-1228            AK044360.1         896-1230
            1229-1401           AK014813.1         1230-1402
            1402-5534           BC059230.1         93-4225
            5535-5556           AI838753.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 62.65 cM"
     gene            1..5556
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /note="extended synaptotagmin-like protein 2"
                     /db_xref="GeneID:52635"
                     /db_xref="MGI:MGI:1261845"
     exon            1..451
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..2659
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /note="chr2 synaptotagmin; E-Syt2; family with sequence
                     similarity 62 (C2 domain containing), member B; family
                     with sequence similarity 62, member B"
                     /codon_start=1
                     /product="extended synaptotagmin-2"
                     /protein_id="NP_083007.2"
                     /db_xref="CCDS:CCDS26211.1"
                     /db_xref="GeneID:52635"
                     /db_xref="MGI:MGI:1261845"
                     /translation="MSSAGGEGPEAGPGRAGGRSEPEAPGSALSVDLPGLLGQLARSF
                     ALLLPVYALGYLGLSFSWVLLALGLLAWCRRSRGLKASRLCRALALLEDEEQAVRLGV
                     RACDLPAWVHFPDTERAEWLNKTVKHMWPFICQFIEKLFRETIEPAVRGANAHLSTFS
                     FTKVDVGQQPLRVNGVKVYTENVDKRQIILDLQISFVGNCEIDLEIKRYFCRAGVKSI
                     QIHGTMRVILEPLIGDMPLVGALSIFFLRKPLLEINWTGLTNLLDIPGLNGLSDTIIL
                     DIISNYLVLPNRITVPLVSEVQIAQLRFPIPKGVLRIHFIEAQDLQGKDTYLKGLVKG
                     KSDPYGIIRVGNQIFQSKVIKENLSPKWNEVYEALVYEHPGQELEIELFDEDPDKDDF
                     LGSLMIDLIEVEKERLLDEWFTLDEVPKGKLHLKLEWLTLMPDAANLDKVLADIRADK
                     DQASDGLSSALLILYLDSARNLPSGKKINSNPNPLVQMSVGHKAQESKIRYKTSEPVW
                     EENFTFFIHNPRRQDLEVEVKDEQHQCSLGSLRIPLSQLLTSDNMTINQRFQLSNSGP
                     NSTLKMKIALRVLHLEKQERPPDYQHSAQVKRPSVSKEGRKMPIKSQMSASPGTGGAN
                     TAPSTPVMGVDDKPAMEEKPQPPEASPLGHRDLGRSSSSLLASPSHIAAKEPTPSIAS
                     DISLPIATQELRQRLRQLENGTTLGQSPLGQIQLTIRHSSQRNKLIVVVHSCRNLIAF
                     SEDGSDPYVRMYLLPDKRRSGRRKTHVSKKTLNPVFDQSFDFSVSLPEVQRRTLDVAV
                     KNSGGFLSKDKGLLGKVLVVLASEELAKGWTQWYDLTEDGTRPQVIT"
     exon            452..493
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            494..628
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            629..705
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            706..778
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            779..868
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            869..924
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1045
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1222
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1305
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1354
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1444
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1540
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1615
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1702
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1852
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..2226
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2325
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2457
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            2458..2563
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2613
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..5541
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5512..5517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /note="hexamer: AATAAA"
     polyA_site      5534
                     /gene="Esyt2"
                     /gene_synonym="2410017M09Rik; 4921504I16Rik; CHR2SYT;
                     D12Ertd551e; Fam62b"
                     /note="major polyA site"
ORIGIN      
        1 gtggagcggt ggagcccgcg gtccccctca gtggggcgct ggcgcgcggg ctagagcgac
       61 ctgcggtccg tgggagagcc gcgcccgcct cggcagggag gagtgctcag cgcggctgga
      121 gatgagtagc gcggggggcg aggggccgga ggccggtcct ggccgggctg ggggccgctc
      181 cgagcccgag gccccgggca gcgctctgag tgtggacttg ccggggctgc tggggcagct
      241 ggcgcgcagc ttcgcgctgc tgctgccggt gtacgccctg ggctacctgg ggctgagctt
      301 cagttgggtg ctgctggcgc tcgggctgct cgcctggtgc cgccggagcc gcggcctcaa
      361 ggccagccgc ctgtgccgcg cgctggcgct gttggaggac gaggagcaag ctgtgcgcct
      421 gggagtgcgc gcctgcgacc tgcccgcctg ggttcatttt ccagacactg aaagagcaga
      481 atggctaaat aagactgtaa agcacatgtg gccctttatt tgtcagttta tcgagaagct
      541 ctttcgagaa accatagagc cagctgtgcg tggagcaaat gctcacctca gcaccttcag
      601 cttcacaaaa gtggatgtgg gtcagcagcc cctgagagtc aatggtgtta aggtttatac
      661 tgaaaatgta gacaaaagac aaattattct ggaccttcag attagttttg taggaaattg
      721 tgagattgat ttggagatca aaagatattt ttgtagagct ggtgtgaaaa gtattcagat
      781 ccatgggaca atgcgggtga tactggagcc cctgattgga gacatgcctt tagttggagc
      841 tttgtccatc ttcttcctta ggaaaccact tttagagatt aactggacag ggctgactaa
      901 ccttctggac atccctggac tgaatggttt atctgatact atcattttgg atataatatc
      961 aaactatcta gtgcttccca atcgaatcac cgttcctctt gtcagtgaag ttcaaatagc
     1021 tcagctgcgg tttcccatac caaagggtgt cctaaggatt cactttattg aagctcaaga
     1081 tcttcagggc aaagacacct accttaaagg ccttgtcaag ggaaaatcag acccctatgg
     1141 aattatccgg gtgggcaacc aaatcttcca gagcaaagtc atcaaagaga accttagtcc
     1201 aaaatggaat gaagtgtatg aggctttagt gtatgaacac cctggacaag aattggagat
     1261 tgaactgttt gatgaagatc cagacaagga tgatttctta ggaagtctta tgattgatct
     1321 tattgaagtt gagaaggagc gccttttaga tgaatggttc actctggacg aggttcccaa
     1381 aggaaagctg catttgaagt tagagtggct cacactgatg cctgatgctg caaaccttga
     1441 caaggtgctg gcagacatca gagctgacaa ggaccaagcc agtgatggcc tttcatctgc
     1501 actgctgatc ttgtatttgg attctgcaag gaaccttccg tcagggaaga aaataaacag
     1561 caacccaaat cctcttgtcc agatgtcagt cggtcacaag gctcaagaga gtaagattcg
     1621 atacaaaact agtgagcctg tatgggagga aaacttcact ttcttcattc ataaccccag
     1681 gcgccaggac cttgaggttg aggttaaaga tgagcagcat cagtgttctc tggggagcct
     1741 gaggatccca ctcagtcaac tgcttacaag tgacaacatg actataaacc agcggttcca
     1801 gcttagcaac tcgggtccaa acagcacctt aaaaatgaag attgccctcc gggtcctcca
     1861 tcttgaaaag caagaaaggc ctccagacta ccagcattca gctcaagtga aacggccctc
     1921 tgtctccaaa gaagggagga aaatgcctat caaatctcag atgtctgcgt cgccaggcac
     1981 cggtggtgcc aacactgctc cgtcaacacc agtcatggga gtcgatgata agcctgccat
     2041 ggaggagaag ccccagcccc ctgaggccag ccctctcggg catcgtgacc taggcaggag
     2101 ctcctccagc ctcttggcct ctccaagcca catcgcagcc aaggaaccca ccccaagcat
     2161 tgcctcagac atatcactgc ccattgccac tcaggagctg agacaaaggc tacgtcaact
     2221 tgaaaatggg acaaccctgg gacaatctcc tctggggcag atccagctga ctatccggca
     2281 cagctcccaa aggaataagc tcatcgtggt tgtgcactcc tgcagaaacc tcattgcctt
     2341 ctcggaagat ggctctgacc catatgtccg catgtattta ttaccagaca agagacgctc
     2401 aggaagaagg aaaacacatg tatcaaagaa gacattgaac cctgtgtttg atcagagctt
     2461 cgacttcagt gtttcgctgc ctgaggtgca aaggagaaca ttggatgtgg ctgtgaagaa
     2521 cagtggtggc ttcctgtcca aagataaagg gcttcttggc aaagtgctgg ttgttctggc
     2581 atctgaagaa ctcgccaagg gctggaccca gtggtatgac ctcacagaag atgggacaag
     2641 gccacaggtg ataacgtagc tacgctggac gtaggtctat ttttcagtgt atcacgtggc
     2701 acctcaacat ggaacacacc ttctcgcaga tgtaccaatg ctatttttat aattttaagt
     2761 attgagttat acatacctta ataattttat aaaacttggc attgtaggaa aatttggcca
     2821 atattattgt tgaatctccc tgttgagctt cctccagtgc ttcaccagtg gtgtagtgat
     2881 gtgtttcctg tgctgtgtgg agctctgtca gctgcacctg ctggaaagca gatacctgga
     2941 cttgtttctg ccttgtcctg catcctggag gtgcactaca ccatgtaaat agattctttt
     3001 ttataatatc tacatgctgg tttggataaa gaagaaaatg aattaaggaa atatgtattt
     3061 tcttctaaga cttattacat tctgtgacaa attaatcatt ttcatctttt cagcaaaatg
     3121 ttctcataga tctgttgtgt gatatggctt tttaaaggaa aaaatggaaa gattattaaa
     3181 tattagaatt tttatgtcta ttatgaacga taaaacaaat ttcaaattac taacattatt
     3241 ataaatagga aaatacagtg agaagtcagt gaatggctaa ttttaaaagt tgtataactg
     3301 agtactggac aataccgaga gcattgtcaa ggctctggag aatcacagta tactcatctg
     3361 aaatgtatta cataagaaag gaatgtgctg tttatttgtg taattgtttg ttataacaga
     3421 ctcatgagta gtagagagat gcacacatgc taggcgttgt acctcacatc tgtaatccca
     3481 gggtatggga ggccacacca agaagaccca agtgttaaag gccagcctgg gctacacctc
     3541 aaaactctgt cttgggaaaa agaaacatac attatatttt gcccaaagca tattaggata
     3601 taaggtgtta tcaagagtga tgcaagctta atttgttttg tccttgtttc ttaccctagg
     3661 aagacaggtc tgttgttact gtaaagaggt gaaatgggat ggatgcatag taacaaaggg
     3721 tgctgagcta aagacgttag ttagagcact gcacttgctc ttgatgggag aacctggcca
     3781 taagtccaca cacaggacag acaccttccc gagcttcccc tcatacgtga caaaagatcc
     3841 tgcgcagtgc cagcaagctg ctgtaaggct tgagatttcc tggatatcct cattttgtgc
     3901 catgccagat gttaatacaa tttttatttt ggaatccttt tatttttaga ttgggaaaag
     3961 catgttttgt atgtaagaga tctcaaacct cttgtgaggt gaggctatgt cacagtatta
     4021 taagctatgc ttgtctgtaa gcaaaagatg cacaaatgta ggaagatttt tttttcagta
     4081 tgcattttaa cctctaggat gaaaagggct tctcagctat gagatcattt aaaactatgt
     4141 acacttatag caggaaaata cagctttgta atcaaaattg gagtagttcc attcacatgc
     4201 ttcttggaat taaacctatg ggaagcttat gtgaccaaac ataccaaagt gcctgtgatg
     4261 aatatcgagt gctccttttg cactctcctc ctgtctaaaa gatgaaagtg tgcctcagtt
     4321 tgtaatgtaa cacgtacagt tgtaacacag tctgtggctc ttcacagcac accccaggta
     4381 gtcataccgt cagagtaatc tctctcctgc catctgacac cttcctctga atcctgttta
     4441 taaatctttt ttcattagtg agtaggattg tgagaaagtt tgtgtaggta cacacatgaa
     4501 catatatgca cataaaatat tcaagggtgg cattttagtt gcaggagaaa ggcaggttat
     4561 attaaaacta gttgcttgac catggtatta gctttttttt tccttaagtg tgtttttaat
     4621 taagtccatt taagagcttc tgtctataaa agagattttt ttcctgaaat tgtatcatgg
     4681 caatcgtttt ttcaccaaca acctatctat cttagttttg catccataga cttagttaag
     4741 tgtgtatttt atttaatgga atgcttttca taactaatta ggcaaattga gtagagttaa
     4801 gggtacaatc ccctaataaa tcagtatgat ccctactaca gtcacaaaag ctggtgccaa
     4861 tgagttcaga tacagtgccc ccacgtgcat catctgagtc ttcaagtagc atctccagtc
     4921 taagtggaaa ggagacagaa ggtctagtaa tactagtcca cttagttgta gttctgagtg
     4981 ctgcatgagg agggctgact tgccatcaag gcacactcca gtgtcctaaa gatataaaat
     5041 agatagatac ttttagttct caaaatgtta acttttagtt tttaatgact tacctaggaa
     5101 aaaagaatgt tacattttct actttgagcc aaaccaatga gggaaattag tgatttgaag
     5161 tacagctctc ctaacttatt catgtggtgg cttatcctgt gccttaaagt acaaaggtgt
     5221 tttcaagttt caggtgaaaa cagaagcagc cttgcaacca tatgtactac catttgtatt
     5281 ttgcactcta tatttcttgt acaggcagtc cctatgcaga ggtgtttagg attagtttaa
     5341 aaaacctcag gcagatacag agagtgcagc ctgctgcagt gaatggtccc atctggatta
     5401 cccactgtag ggtaatagag ggtggttctt gttttttgtt gtttgtttgt ttgttttaat
     5461 catagaatgt cctttttgct taattttata taacagcaca tgtatttatt caataaaact
     5521 tttatgttag tgtactcgta aaaaaaaaaa aaaaaa
//
