LOCUS       NM_001014976            6630 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus extra spindle pole bodies 1, separase (Espl1),
            transcript variant 1, mRNA.
ACCESSION   NM_001014976 XM_484508
VERSION     NM_001014976.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Chen,J., Gao,C., Luo,M., Zheng,C., Lin,X., Ning,Y., Ma,L., He,W.,
            Xie,D., Liu,K., Hong,K. and Han,C.
  TITLE     MicroRNA-202 safeguards meiotic progression by preventing premature
            SEPARASE-mediated REC8 cleavage
  JOURNAL   EMBO Rep 23 (8), e54298 (2022)
   PUBMED   35712867
  REMARK    GeneRIF: MicroRNA-202 safeguards meiotic progression by preventing
            premature SEPARASE-mediated REC8 cleavage.
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Nikalayevich,E., El Jailani,S., Dupre,A., Cladiere,D.,
            Gryaznova,Y., Fosse,C., Buffin,E., Touati,S.A. and Wassmann,K.
  TITLE     Aurora B/C-dependent phosphorylation promotes Rec8 cleavage in
            mammalian oocytes
  JOURNAL   Curr Biol 32 (10), 2281-2290 (2022)
   PUBMED   35385691
REFERENCE   3  (bases 1 to 6630)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6630)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   5  (bases 1 to 6630)
  AUTHORS   Radonova,L., Pauerova,T., Jansova,D., Danadova,J., Skultety,M.,
            Kubelka,M. and Anger,M.
  TITLE     Cyclin A1 in Oocytes Prevents Chromosome Segregation And Anaphase
            Entry
  JOURNAL   Sci Rep 10 (1), 7455 (2020)
   PUBMED   32366979
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6630)
  AUTHORS   Goldring,K., Jones,G.E., Thiagarajah,R. and Watt,D.J.
  TITLE     The effect of galectin-1 on the differentiation of fibroblasts and
            myoblasts in vitro
  JOURNAL   J Cell Sci 115 (Pt 2), 355-366 (2002)
   PUBMED   11839787
REFERENCE   7  (bases 1 to 6630)
  AUTHORS   Pelton,T.A., Sharma,S., Schulz,T.C., Rathjen,J. and Rathjen,P.D.
  TITLE     Transient pluripotent cell populations during primitive ectoderm
            formation: correlation of in vivo and in vitro pluripotent cell
            development
  JOURNAL   J Cell Sci 115 (Pt 2), 329-339 (2002)
   PUBMED   11839785
REFERENCE   8  (bases 1 to 6630)
  AUTHORS   Jager,H., Herzig,A., Lehner,C.F. and Heidmann,S.
  TITLE     Drosophila separase is required for sister chromatid separation and
            binds to PIM and THR
  JOURNAL   Genes Dev 15 (19), 2572-2584 (2001)
   PUBMED   11581162
REFERENCE   9  (bases 1 to 6630)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 6630)
  AUTHORS   Mei,J., Huang,X. and Zhang,P.
  TITLE     Securin is not required for cellular viability, but is required for
            normal growth of mouse embryonic fibroblasts
  JOURNAL   Curr Biol 11 (15), 1197-1201 (2001)
   PUBMED   11516952
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163291.3.
            
            On May 22, 2009 this sequence version replaced NM_001014976.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC082603.1, AK129072.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AC163291.3         129592-129714
            124-213             AC163291.3         130059-130148
            214-1269            AC163291.3         131483-132538
            1270-1374           AC163291.3         132718-132822
            1375-1486           AC163291.3         133102-133213
            1487-1623           AC163291.3         135911-136047
            1624-1817           AC163291.3         137251-137444
            1818-2057           AC163291.3         138225-138464
            2058-2202           AC163291.3         138964-139108
            2203-2341           AC163291.3         139197-139335
            2342-2481           AC163291.3         139534-139673
            2482-2616           AC163291.3         142497-142631
            2617-2736           AC163291.3         143899-144018
            2737-2908           AC163291.3         145714-145885
            2909-3037           AC163291.3         146235-146363
            3038-3194           AC163291.3         146482-146638
            3195-3304           AC163291.3         147286-147395
            3305-4293           AC163291.3         148487-149475
            4294-4666           AC163291.3         149818-150190
            4667-4828           AC163291.3         150349-150510
            4829-5042           AC163291.3         152711-152924
            5043-5233           AC163291.3         153002-153192
            5234-5361           AC163291.3         153712-153839
            5362-5484           AC163291.3         153943-154065
            5485-5640           AC163291.3         155624-155779
            5641-5811           AC163291.3         155875-156045
            5812-5916           AC163291.3         156167-156271
            5917-6032           AC163291.3         156454-156569
            6033-6116           AC163291.3         156749-156832
            6117-6281           AC163291.3         156942-157106
            6282-6630           AC163291.3         157307-157655
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 57.45 cM"
     gene            1..6630
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /note="extra spindle pole bodies 1, separase"
                     /db_xref="GeneID:105988"
                     /db_xref="MGI:MGI:2146156"
     exon            1..123
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            124..213
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     CDS             133..6489
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /EC_number="3.4.22.49"
                     /note="Cut1/ESP1 related protein; caspase-like protein
                     ESPL1; extra spindle poles-like 1 protein; separase"
                     /codon_start=1
                     /product="separin"
                     /protein_id="NP_001014976.1"
                     /db_xref="CCDS:CCDS27878.1"
                     /db_xref="GeneID:105988"
                     /db_xref="MGI:MGI:2146156"
                     /translation="MRNFKGVNFATLLCSKEETQQLLPDLKEFLSRSRTDFPSSRTDA
                     ERRQICDTILRACTQQLTAKLDCPGHLRSILDLAELACDGYLLSTPQRPPLYLERILF
                     ILLRNGSTQGSPDTVLRLAQPLHACLVQNSGEAAPQDYEAVTRGSFSLFWKGAEALLE
                     RRAAFSTRLNALSFLVLLEDGSVPCEVPHFASPTACRLVAAYQLYDATGQGLDEADAD
                     FLYEVLSRHLIRVLVGEGGSSPGPLSPQRALCLLEITLEHCRRLCWNHHHRQAARAVE
                     RARNHLEKTSVAPSLQLCQMGVELLEAVEERPGAVAQLLRKAAAVLINSIEAPSPPLR
                     ALYDSCQFFLSGLERGIRRHCGLDAILSLFAFLGGYSSLVRHLREVSEASSKQQQCLL
                     QMHFQGFHLFTGIVYDFAQGCQATELAQLVDGCRSAAVWMLEALEGLSGGELADYLSM
                     TASYTSNLAYSFFSQKLYEEACVISEPVCQHLGSATSGACPEVPPEKLHRCFRLHVES
                     LKKLGKQAQGCKMVTLWLAALKPYSLEHMVEPVTFWVRVKMDASRAGDKELQLQTLRD
                     SLSCWDPETQSLLLREELRAYKSVRADTGQERFNIICDLLELSPEETAAGAWARATYL
                     VELAQVLCYHNFTQQTNCSALDAVQEALQLLESVSPEAQEQDRLLDDKAQALLWLYIC
                     TLEAKMQEGIERDRRAQAPSNLEEFEVNDLNYEDKLQEDRFLYSSIAFNLAADAAQSK
                     CLDQALTLWKEVLTKGRAPAVRCLQQTAASLQILAAVYQLVAKPLQALETLLLLQIVS
                     KRLQDHAKAASSSCQLTQLLLNLGCPSYAQLYLEEAESSLRSLDQTSDACQLLSLTCA
                     LLGSQLCWACQKVTAGVSLLLSVLRDPALQKSSKAWYLLRVQALQVLAFYLSLSSNLL
                     SSALREQLWDQGWQTPETALIDAHKLLRSIIILLMGSDVLSIQKAATESPFLDYGENL
                     VQKWQVLTEVLTCSERLVGRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKG
                     ELELARGDIDLCQSDLQQVLFLLESSTEFGVVTQHPDSVKKVHTQKGKHKAQGPCFPP
                     LSEEEPFLKGPALELVDTVLNEPGPIQSSVNSSPVLKTKPPPNPGFLSHLPSCDCLLC
                     ASPALSAVCLRWVLVTAGVRLATGHKAQGLDLLQAVLTRCPAATKRFTQSLQASLNHR
                     TTPSCVPSLFDEIMAQVYTHLALEFLNQTSEKSLGKVLASGLKFVATRIQSLEIWRAH
                     LLLVQALAKLAHFSCCTSELFASSWGWHPPLVKSLPVLEPAKIRRQKCSGRGRRRIAS
                     VPPPLHNSSQKGLEEEGPPCTPKPPGRARQAGPRVPFTIFEEVHPTKSKLQVPLAPRV
                     HRRAQTRLKVIFSDDSDLEDLVSADTQLVEEPKRRGTASRTRGQTRKGRSLKTDAVVA
                     IESTPGHSSVSGRTRRARKVASRNCEEESPKAPLCVWASQGPEIMRSIPEEEPVDNHL
                     EKSFEILRGSDGEDSASGEKAAAADTGLPVGECEVLRRDSSKAERPVLYSDTEANSDP
                     SPWLPPFSVPAPIDLSTLDSISDSLSIAFRGVSHCPPSGLYAHLCRFLALCLGHRDPY
                     ATAFLVAESISITCRHQLLTHLHRQLSKAQKQQESPELAEHLQRLDLKERPGGVPLAR
                     IQRLFSFKALGSGCFPQAEKESFQERLALIPSGVTVCVLALATLQPGTLSNTLLLTRL
                     EKDNPPITVKIPTAQNKLPLSAVLKEFDAIQKDQKENSSCTEKRVWWTGRLALDQRME
                     ALITALEEQVLGCWRGLLLPCSADPSLAQEASKLQELLRECGWEYPDSTLLKVILSGA
                     RILTSQDVQALACGLCPAQPDRAQVLLSEAVGQVQSQEAPRSQHLVLVLDKDLQKLPW
                     ESTPILQAQPVTRLPSFRFLLSYTVTKEAGASSVLSQGVDPQNTFYVLNPHSNLSSTE
                     ERFRASFSSETGWKGVIGEVPSLDQVQAALTERDLYIYAGHGAGARFLDGQAVLRLSC
                     RAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQ
                     GWLGAGPGAPFLYYASQARQAPRLKYLIGAAPVAYGLPISLQTP"
     exon            214..1269
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1374
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1486
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1623
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1817
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..2057
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2202
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2341
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2342..2481
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2616
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2736
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..2908
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            2909..3037
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3194
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            3195..3304
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            3305..4293
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            4294..4666
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            4667..4828
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            4829..5042
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5043..5233
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5234..5361
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5362..5484
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5485..5640
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5641..5811
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5812..5916
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            5917..6032
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            6033..6116
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            6117..6281
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
     exon            6282..6630
                     /gene="Espl1"
                     /gene_synonym="Cerp; ESP1; PRCE; SSE"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgaagcgtgc ccacccggtg cttggtggag ctcgggctgg aggacgctgc taggccggtg
       61 aggaacggca gcttcgaggg aattaaggca cggagggtct ggtgagcgcg cgctcaagcc
      121 gcgacttttg ccatgaggaa cttcaaagga gtcaacttcg ccactctgct ctgcagcaag
      181 gaggagaccc agcagctgct gcctgacttg aaggagttcc tgtccagatc ccggactgat
      241 ttccccagca gccgaacaga tgccgaaaga agacaaattt gcgataccat cctgagggct
      301 tgcacccagc agttgactgc caagctcgat tgccctgggc acctgaggag tattttggac
      361 ctggcagagc tggcctgtga tggctatttg ttgtctactc cacagcgccc tcccctctac
      421 ctggaacgca ttctcttcat cctacttcgc aatggttcca ctcagggaag cccagacact
      481 gtgctgcgcc ttgcccagcc tctccatgcc tgcttggttc agaactctgg agaggctgcc
      541 cctcaggact atgaggccgt gacccggggc agcttctctc tgttttggaa gggagcagag
      601 gcactgttgg agcggcgagc tgcgttctca actcggctga atgccttgag cttcttggtg
      661 ctcttggagg atggcagtgt cccctgtgag gtccctcact ttgcttctcc aaccgcctgt
      721 cgtttagttg ccgcctatca gctgtacgat gctacaggac aaggtctaga tgaagctgat
      781 gccgacttcc tgtatgaagt gctttctagg cacctgatca gagtcttagt aggtgagggt
      841 ggtagctctc ccggtcctct ctcgcctcag agggccctct gcctcttgga aatcaccctg
      901 gaacactgcc gtcggctctg ctggaaccac caccaccgcc aggctgccag agcagtggag
      961 agggctcgta atcacctaga gaagaccagt gtagctccta gcctccagtt atgccagatg
     1021 ggggttgagc tgttggaagc tgtagaagaa agaccagggg cagtggccca gcttctgagg
     1081 aaggcagcag ccgttcttat caacagtatc gaggcgccgt cgcccccgct ccgggcattg
     1141 tacgacagct gccagttctt cctttcaggc ctggagagag gcatcaggag gcactgtgga
     1201 cttgatgcca tattgagtct ctttgctttt cttgggggat acagctccct tgtccggcac
     1261 ctgagagaag tctctgaagc atcttccaag cagcagcagt gcttgcttca gatgcacttc
     1321 cagggtttcc acctcttcac cgggattgtg tatgactttg ctcaaggctg tcaggcaact
     1381 gagcttgctc agctagtgga cggttgcaga tctgctgctg tctggatgtt ggaggcctta
     1441 gagggcctgt cgggtggaga gctggctgac tacctgagca tgactgcctc ttacaccagc
     1501 aacttggcct acagcttctt tagtcagaag ctctatgagg aggcctgcgt catctcagag
     1561 ccagtctgtc agcacttggg ctcggcaacg tcgggtgctt gtcctgaagt gcctcctgag
     1621 aagctacata ggtgcttcag gctgcatgtg gagagtttga agaaactggg taaacaggcc
     1681 cagggctgca agatggtgac cttatggctg gcagctctga aaccctacag tctggaacac
     1741 atggtcgagc cagtcacttt ctgggttcga gtcaaaatgg atgcatccag ggcaggagac
     1801 aaggagctac aacttcagac tttgcgagac agcctaagct gctgggaccc agagacacag
     1861 tctctgcttc tcagggagga gctgcgggcc tacaagtcag tacgggctga cactgggcag
     1921 gagcgcttca acatcatctg tgatctgctc gagctgagcc ctgaagagac agcagctgga
     1981 gcctgggcac gagctaccta cctggtggaa ctggcccaag tgctgtgcta ccacaacttt
     2041 acccagcaga ccaactgctc tgccttggat gctgtccagg aagccctgca gcttctggag
     2101 tccgtgagtc ctgaggccca ggagcaggat cgacttctgg atgataaagc acaggccttg
     2161 ttatggcttt acatctgtac cttagaggcc aagatgcagg aaggtattga gcgggatcgg
     2221 agagcccagg cccctagtaa cttggaggaa tttgaagtca atgacctgaa ctatgaagat
     2281 aaactgcagg aagatcgttt tctgtatagt agcattgcct tcaatctggc tgctgatgca
     2341 gctcagtcca aatgcctgga ccaggccctg actctgtgga aagaagtgct caccaagggg
     2401 cgagccccag ctgtgcggtg tctccagcag actgcagcct ccctgcaaat cctagcggcc
     2461 gtctatcagt tggtggcaaa gcccttgcag gctctggaga ccctcctgct cctgcagatt
     2521 gtatctaaga gactgcagga ccacgcaaag gcagccagct cttcctgcca gcttacccag
     2581 ctactcttga accttggctg tcccagctat gcgcagttgt atctggaaga ggcggagtca
     2641 agcctgagga gcctggacca gacaagtgac gcgtgccagc tgctgtccct gacctgtgct
     2701 ctgcttggaa gtcagctctg ctgggcttgc cagaaggtaa ctgcaggcgt ttctttgttg
     2761 ctttctgtgc ttcgggatcc tgccctccag aagtcatcta aggcttggta cttgctacgt
     2821 gtccaggccc tgcaagttct ggctttttac ctcagtctct cgtccaacct cctctcaagt
     2881 gccctgcgtg agcagctctg ggatcaaggc tggcagaccc cggagacggc gctgattgat
     2941 gcccacaagc tcctcagaag catcatcatt ctgctcatgg gaagtgatgt gctctccatt
     3001 cagaaagcag ccaccgagtc gccctttctg gactacggtg agaatctggt gcaaaaatgg
     3061 caggttctca cagaggtgct gacctgctcg gagaggctgg tcggccgcct gggtcgcctg
     3121 gggaacgtga gtgaggccaa ggccttttgc ttggaggccc taaaacttac gactaagctg
     3181 cagatacctc gacagtgtgc cctgttcctt gtactgaagg gtgagctgga gctggcccgg
     3241 ggtgacattg acctctgtca gtcagacttg cagcaggttc tcttcttgct cgagtcttcc
     3301 acagagtttg gtgttgtgac ccagcatcca gactcagtga agaaggtgca cacgcagaaa
     3361 gggaagcata aggctcaagg cccatgtttc ccacccctgt cagaggagga gcccttcctg
     3421 aaaggtcctg cccttgagct ggtggacact gtgctcaacg aacctggtcc catccagtcc
     3481 tctgtaaact cctccccagt cctgaaaacc aagccaccac ccaaccctgg cttcctgtct
     3541 cacttgccca gctgtgactg cttactgtgt gccagccctg ccctctcagc agtctgtctg
     3601 cgctgggtgc tggtcactgc cggagtgagg ctggccaccg gtcacaaagc tcagggtctg
     3661 gatctgctgc aagctgtgct gacgcgttgc cctgcagcca ccaagcgctt cacacagagt
     3721 ctccaagctt ctctgaatca cagaacaacc ccctcttgtg ttcccagcct ctttgatgag
     3781 atcatggctc aagtgtacac gcacttggca ttggagttcc tgaatcagac atctgaaaag
     3841 agcctgggga aggtcctggc gtcggggctg aagtttgtgg caacacggat acagagcttg
     3901 gagatctgga gagcccacct gctcttggtt caggcccttg caaagctggc ccacttcagc
     3961 tgctgtactt ccgaactctt tgcaagctcc tggggctggc acccaccatt agtaaaaagt
     4021 ctgccagtct tagagcctgc taaaattcgg agacaaaaat gctctggacg gggccgccgg
     4081 cggatagcct ctgttccccc acccctccat aacagctctc agaaaggtct ggaagaggaa
     4141 gggccacctt gtacccctaa gcctccaggt cgggctaggc aagctggccc tcgtgtccct
     4201 ttcaccatat ttgaagaagt ccaccccacc aagagcaagc tccaggttcc cttggctccc
     4261 agggtccaca ggcgggccca gactcgcctc aaggtgatct tcagtgatga cagtgacctg
     4321 gaagaccttg tctcagctga cacacaactt gtagaggagc ctaagaggcg agggactgct
     4381 tcccgtaccc ggggccaaac taggaaggga cgtagcttaa agacagatgc ggtggttgct
     4441 atagaaagta cccctggaca ttccagtgtc agtgggagga ctcggagggc caggaaggtg
     4501 gcttcaagaa attgtgaaga agagagtccc aaagcacccc tctgtgtctg ggccagccag
     4561 ggacccgaga tcatgagaag tatccctgaa gaggagccag tggacaacca tctggaaaaa
     4621 agcttcgaga ttctcagggg ttctgatggg gaagactcgg cctcaggtga gaaggcggca
     4681 gctgcagaca ctggtctacc cgtaggagag tgtgaggtgt tgagacggga ctccagcaag
     4741 gcagagagac ctgtcttgta ctcagacacg gaggccaata gtgaccctag tccttggctc
     4801 ccacccttct cagttcctgc acccattgat ctttctaccc tggattccat ttctgactca
     4861 ttgagcattg ctttccgtgg tgttagtcac tgccctccaa gtgggctcta tgctcacctc
     4921 tgccgcttct tggccctgtg tctgggccac cgagatcctt atgccactgc gttccttgtt
     4981 gctgagtcta tctccatcac ctgccgtcac cagctgctca cccacttgca ccgacagctc
     5041 agcaaggccc agaagcagca agaatcacct gaactggcag agcatctgca gaggctggac
     5101 ctgaaggaga ggcccggagg tgtcccactg gcccgtatcc agcgcctctt ttcctttaag
     5161 gctttgggat ctggctgctt cccccaggca gagaaggaga gtttccagga gcgcctggct
     5221 ctgatcccta gtggggtgac tgtctgtgtg ttggctctgg ccaccctcca gcctggaacc
     5281 ttgagcaaca ctcttttact gacccgcctg gaaaaggaca accccccaat tactgtgaag
     5341 atccccactg cccaaaataa gctccctcta agcgcagtgc tgaaagagtt tgatgccatc
     5401 cagaaggacc aaaaagaaaa cagcagctgt actgagaaac gagtatggtg gacgggccgg
     5461 ctggcactgg accaaaggat ggaggcactc atcaccgccc tggaggaaca ggtgctgggc
     5521 tgctggaggg ggctgctgct gccatgcagt gctgacccca gccttgccca ggaggcctcc
     5581 aaactacaag agctgctgcg ggaatgtggc tgggaatatc ctgactccac tctcctgaaa
     5641 gtcatcctta gtggggccag aatcctcacc agccaggatg ttcaggcctt ggcgtgtggg
     5701 ctgtgcccag cccagcctga ccgagcccaa gtgctcctga gcgaggcagt aggacaggtt
     5761 cagagccagg aggcccctcg aagtcaacat cttgtattgg tgttagacaa ggacctgcaa
     5821 aagctgcctt gggaaagcac gcccatcctc caagcacagc cggtcacccg gctgccctcc
     5881 ttccgcttcc tgctcagcta cacagtcact aaagaggctg gagcctcatc agtgctgagc
     5941 caaggtgttg atccacagaa taccttctat gtgctaaacc ctcacagtaa cctgtcgagc
     6001 acagaggaga gatttcgagc cagttttagc agtgaaactg gctggaaagg agtgattggg
     6061 gaagtgccaa gcctcgatca ggtgcaggct gccctgacag agcgggattt atatatctac
     6121 gcagggcacg gagccggtgc ccgcttcctt gatgggcagg ctgtcctgcg gctgagctgc
     6181 cgggcggtgg ccctgctgtt cggctgcagc agtgcagccc ttgccgtgca tggaaacctg
     6241 gagggagctg gtatcgtgct caagtacatc atggctggct gccccttgtt tctaggtaat
     6301 ctctgggatg tgactgaccg tgacattgac cgttacacag aggctttgct gcagggctgg
     6361 ctgggagcag gcccaggggc tccctttctc tactatgcca gccaagcccg ccaggcccct
     6421 cgactcaagt acctcattgg tgctgcacct gtagcctacg gcctacctat ctctctgcag
     6481 acaccctgaa gctgtcttgt ctatggtaga agctgtgtga ctacctccaa acttggtttt
     6541 gattatttag aatttttaaa gtgattttcc ctggtgtttt gtatgaaata ttttctttaa
     6601 tgtgacctta ataaagatat tccaagcctc
//
