LOCUS       NM_001164360            3153 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus ER lipid raft associated 1 (Erlin1), transcript
            variant 3, mRNA.
ACCESSION   NM_001164360
VERSION     NM_001164360.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Wright,F.A., Bonzerato,C.G., Sliter,D.A. and Wojcikiewicz,R.J.H.
  TITLE     The erlin2 T65I mutation inhibits erlin1/2 complex-mediated
            inositol 1,4,5-trisphosphate receptor ubiquitination and
            phosphatidylinositol 3-phosphate binding
  JOURNAL   J Biol Chem 293 (40), 15706-15714 (2018)
   PUBMED   30135210
  REMARK    GeneRIF: The erlin2 threonine to isoleucine 65 substitution
            inhibits erlin1/erlin2 complex-mediated inositol
            1,4,5-trisphosphate receptor ubiquitination and
            phosphatidylinositol 3-phosphate binding.
REFERENCE   3  (bases 1 to 3153)
  AUTHORS   Huber,M.D., Vesely,P.W., Datta,K. and Gerace,L.
  TITLE     Erlins restrict SREBP activation in the ER and regulate cellular
            cholesterol homeostasis
  JOURNAL   J Cell Biol 203 (3), 427-436 (2013)
   PUBMED   24217618
REFERENCE   4  (bases 1 to 3153)
  AUTHORS   Pednekar,D., Wang,Y., Fedotova,T.V. and Wojcikiewicz,R.J.
  TITLE     Clustered hydrophobic amino acids in amphipathic helices mediate
            erlin1/2 complex assembly
  JOURNAL   Biochem Biophys Res Commun 415 (1), 135-140 (2011)
   PUBMED   22020079
  REMARK    GeneRIF: 2 MDa erlin1/2 complex is composed of an assemblage of
            lower-order hetero-oligomers, probably heterotrimers, linked
            together by assembly domain hydrophobic residues.
REFERENCE   5  (bases 1 to 3153)
  AUTHORS   Pearce,M.M., Wormer,D.B., Wilkens,S. and Wojcikiewicz,R.J.
  TITLE     An endoplasmic reticulum (ER) membrane complex composed of SPFH1
            and SPFH2 mediates the ER-associated degradation of inositol
            1,4,5-trisphosphate receptors
  JOURNAL   J Biol Chem 284 (16), 10433-10445 (2009)
   PUBMED   19240031
REFERENCE   6  (bases 1 to 3153)
  AUTHORS   Lyssand,J.S., DeFino,M.C., Tang,X.B., Hertz,A.L., Feller,D.B.,
            Wacker,J.L., Adams,M.E. and Hague,C.
  TITLE     Blood pressure is regulated by an alpha1D-adrenergic
            receptor/dystrophin signalosome
  JOURNAL   J Biol Chem 283 (27), 18792-18800 (2008)
   PUBMED   18468998
REFERENCE   7  (bases 1 to 3153)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   8  (bases 1 to 3153)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ074662.1, AK145990.1, BM205368.2 and BE990958.1.
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. All three variants encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011220.1, AK145990.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                CJ074662.1         2-29
            29-3102             AK145990.1         2-3075
            3103-3133           BM205368.2         4-34                c
            3134-3153           BE990958.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 36.7 cM"
     gene            1..3153
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /note="ER lipid raft associated 1"
                     /db_xref="GeneID:226144"
                     /db_xref="MGI:MGI:2387613"
     exon            1..95
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            96..219
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..1153
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /note="SPFH domain family, member 1; protein KE04 homolog;
                     SPFH domain-containing protein 1; endoplasmic reticulum
                     lipid raft-associated protein 1;
                     stomatin-prohibitin-flotillin-HflC/K domain-containing
                     protein 1"
                     /codon_start=1
                     /product="erlin-1"
                     /protein_id="NP_001157832.1"
                     /db_xref="CCDS:CCDS29842.2"
                     /db_xref="GeneID:226144"
                     /db_xref="MGI:MGI:2387613"
                     /translation="MNMTQARLLVAAVVGLVAILLYASIHKIEEGHLAVYYRGGALLT
                     SPSGPGYHIMLPFITTFRSVQTTLQTDEVKNVPCGTSGGVMIYIDRIEVVNMLAPYAV
                     FDIVRNYTADYDKTLIFNKIHHELNQFCSAHTLQEVYIELFDQIDENLKQALQKDLNT
                     MAPGLTIQAVRVTKPKIPEAIRRNFELMEAEKTKLLIAAQKQKVVEKEAETERKRAVI
                     EAEKIAQVAKIRFQQKVMEKETEKRISEIEDAAFLAREKAKADAEYYAAHKYATSNKH
                     KLTPEYLELKKYQAIASNSKIYFGSNIPSMFVDSSCALKYSDGRTGREDSLPPEEARE
                     PSGESPIQNKENAG"
     exon            220..301
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            302..348
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            349..410
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            411..536
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..610
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            611..669
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            670..761
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            762..851
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            852..931
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     exon            932..3137
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3113..3118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /note="hexamer: AATAAA"
     polyA_site      3133
                     /gene="Erlin1"
                     /gene_synonym="2810439N09Rik; Keo4; Spfh1"
                     /note="major polyA site"
ORIGIN      
        1 agtaccgggc aggtgggcgg ggccggctgt tggcagcagt tggcggggcc gggtggcggc
       61 tgcacgtgcg ggcgggggcg atgcgtcact gaccggagga acaaggatga atatgactca
      121 agcccggctt ctggtggctg cagtggtggg gttggtggcg atcctcctgt acgcctccat
      181 ccacaagatc gaagagggac acttggccgt gtactacagg ggaggagctt tgctaacgag
      241 ccccagtgga ccaggctatc atatcatgtt gcctttcatt acaacattca gatctgtgca
      301 gacaacacta caaacggatg aagttaaaaa tgtgccttgt ggaacaagtg gtggagtcat
      361 gatctatatt gaccgaatag aagtggttaa tatgttggct ccttatgcag tgtttgacat
      421 tgtgaggaac tatactgcag actacgacaa gactttaatc ttcaataaaa tccaccatga
      481 gctgaaccag ttttgcagtg cccacacact tcaagaagtt tacatagaat tgtttgatca
      541 aatagatgaa aacctgaagc aggccctgca aaaagattta aacaccatgg ccccaggtct
      601 cactatccag gctgtgcgtg ttacaaaacc caaaatccca gaagccataa gaagaaattt
      661 tgaattaatg gaggcagaga agacaaaact tctcatagct gcacagaaac aaaaggtggt
      721 ggagaaagaa gctgagacgg agaggaaaag ggctgttata gaagcagaga agattgcaca
      781 agtagcaaaa attcgatttc aacagaaagt gatggagaaa gaaactgaaa aacgcatttc
      841 tgagattgaa gatgctgcgt tcctggcccg agagaaggca aaagcagatg ccgagtatta
      901 cgctgcacac aaatacgcca cctcaaacaa gcacaaactg accccagagt atctggagct
      961 caagaaatac caggccattg cctcaaacag taagatctac tttggcagca acatccccag
     1021 catgtttgtg gactcctcct gtgctctgaa atactctgat ggtaggactg ggagagaaga
     1081 ctcccttccc ccagaggagg cccgtgagcc ctctggagag agccccatcc aaaacaagga
     1141 gaacgcaggt tgatgcaaga ggtggaaatg ttctcccata tcaagatgcg acccaagggg
     1201 ctaagtggga acagtggtta tgtggactcg taagattcac agagaatgtg tgctctgttg
     1261 tgattctctt gtcatagtcc tggtttgcca gctgactaca ggatagaccc agctgtctgg
     1321 cactcaaacg gtctctgcag ccacagtttt atcaagtatc ctgtatgtgt tcctttgtaa
     1381 accggtactc atgaatgagg gaaagtctga tgctaagata ctgcctgcac tggaatgtca
     1441 aacactatat aacaagctgt ggtttttaaa agctattgaa taatgtttac attggtccct
     1501 gaggacatgt gtgctcagac attcaagagc taggaggcca gagagaagac cttcagaaaa
     1561 cggtaagtta aagaagacaa gtgtcatcag acacttggga cccgggctct ctttaaagtc
     1621 tagtcccggc attcctccat gtgattgaca gccagacctc tgggttccca ggaaattatc
     1681 ttccagttga atgaccattt acttgataca aattgtacct ttctgttttt ctagtcaggt
     1741 tggtggcctg cagggacgcg tactttgcca cccgaccaga ggttcctcga agatattccc
     1801 aatcactagt ttattgcgtt aggagactca gagatataga aagcagctga aatttaaggg
     1861 agataaagcc tgcactgcac caaagctacg ggtccctgtg tttcctctat tcagtgatgt
     1921 catcaacctc actgtcccag cccatgtgtg actaaagtgc ccggttttag ccacagacaa
     1981 ctgcttagat gtcacctctt ggctgaccaa agctgggaca gggctttaac cagacatagg
     2041 agcagtgtgc aattcctgat tcactgcaca gtattatgtc ataattgcag gaattatttt
     2101 ttgtttttaa aactggattt ggggcacatt cattcacccc aacacttcta tctaaaggcc
     2161 aaggttctag ggctgctatg gtcactaaca cactgattct ccttaaagta attctcgaag
     2221 tgtggaacaa agtgaccgag acagcatcct cagtcatctt tgtctccttc cctgggatgc
     2281 agataccgaa gttgcttttc caactttcgc ctccgctagg agatcagaaa gaattcttgt
     2341 gacttcctgg gcagccattg aattcatttt ccatgagaag atgacagagt tagcctgtgg
     2401 ctataggaga tcatgtcatc cagacctttt tgcccatcac attaactttc ctggaatatt
     2461 gtgctgcaca ggtagacctg aatctgccca gcttgttgac agctcttgtg tatactgtgt
     2521 tgaagccaga cagaaaagta atggggccac ttctgaaacc tctcagctgt tgatctcaca
     2581 gcagctaaag ggttgtgcca aacattttat taagaaagta aagcccagat ttgaatgggg
     2641 gttttcccta ggccttatag tatagaggca tttgtaatat ggagaaaata atttttctca
     2701 tttaattata gaaattacct tcaaacagat tttgtgttct ttggcccttc aaatactggt
     2761 gttacattgt tgctgcagat aaatgatgat tgtcgtggga tatctggatc actgagctct
     2821 gtgctttcat tcctagagat gtttctcatt cccatttagt gaaatgctgt tgccccaaag
     2881 tgatggttgt gggatttctt accggtcata ggccccggtg aggagcaggg aagcgccatt
     2941 gtgaaagatt aaagaaagca cttccacttg agctccttat ggagtgagct tccctgtgcc
     3001 cactcagtga actaagtctg accatccttc agggacgttc cttttggtaa atatacactg
     3061 taatctttaa gtctaaattt atatgtgaaa gttaactttt tttaaaaacc taaataaaat
     3121 tattttccta tcagtgaaaa aaaaaaaaaa aaa
//
