LOCUS       NM_027089               1287 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus equatorin, sperm acrosome associated (Eqtn),
            transcript variant 1, mRNA.
ACCESSION   NM_027089
VERSION     NM_027089.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ito,C., Yamatoya,K., Yoshida,K., Fujimura,L., Sugiyama,H.,
            Suganami,A., Tamura,Y., Hatano,M., Miyado,K. and Toshimori,K.
  TITLE     Deletion of Eqtn in mice reduces male fertility and sperm-egg
            adhesion
  JOURNAL   Reproduction 156 (6), 579-590 (2018)
   PUBMED   30328350
  REMARK    GeneRIF: EQUATORIN-deficient sperm have significantly reduced
            fertility due to sperm-egg adhesion failure.
            GeneRIF: GeneRIF: EQUATORIN associating with SPESP1 is involved in
            sperm-egg adhesion leading to male fertility.
            GeneRIF: Equatorin associating with Spesp1 is involved in sperm-egg
            adhesion to fusion, which is independent of Izumo1, CD9 and Juno.
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Hao,J., Chen,M., Ji,S., Wang,X., Wang,Y., Huang,X., Yang,L.,
            Wang,Y., Cui,X., Lv,L., Liu,Y. and Gao,F.
  TITLE     Equatorin is not essential for acrosome biogenesis but is required
            for the acrosome reaction
  JOURNAL   Biochem Biophys Res Commun 444 (4), 537-542 (2014)
   PUBMED   24480441
  REMARK    GeneRIF: The fertilization and induced acrosome exocytosis rates of
            Eqtn-deficient sperm were dramatically reduced.
REFERENCE   3  (bases 1 to 1287)
  AUTHORS   Ito,C., Yamatoya,K., Yoshida,K., Fujimura,L., Hatano,M., Miyado,K.
            and Toshimori,K.
  TITLE     Integration of the mouse sperm fertilization-related protein
            equatorin into the acrosome during spermatogenesis as revealed by
            super-resolution and immunoelectron microscopy
  JOURNAL   Cell Tissue Res 352 (3), 739-750 (2013)
   PUBMED   23564009
  REMARK    GeneRIF: The present study aims to clarify the morphology
            associated with the integration of the mouse fertilization-related
            acrosomal protein equatorin (mEQT) into the developing acrosome.
REFERENCE   4  (bases 1 to 1287)
  AUTHORS   Hu,X.Q., Ji,S.Y., Li,Y.C., Fan,C.H., Cai,H., Yang,J.L., Zhang,C.P.,
            Chen,M., Pan,Z.F., Hu,Z.Y., Gao,F. and Liu,Y.X.
  TITLE     Acrosome formation-associated factor is involved in fertilization
  JOURNAL   Fertil Steril 93 (5), 1482-1492 (2010)
   PUBMED   19285662
  REMARK    GeneRIF: Acrosome formation-associated factor (Afaf) might play an
            important role in membrane trafficking during acrosome formation
            and participate in fertilization.
REFERENCE   5  (bases 1 to 1287)
  AUTHORS   Yoshida,K., Ito,C., Yamatoya,K., Maekawa,M., Toyama,Y.,
            Suzuki-Toyota,F. and Toshimori,K.
  TITLE     A model of the acrosome reaction progression via the acrosomal
            membrane-anchored protein equatorin
  JOURNAL   Reproduction 139 (3), 533-544 (2010)
   PUBMED   20032212
  REMARK    GeneRIF: Equatorin-based progression model will be useful for
            analyzing not only the behavior of equatorin but also of other
            molecules of interest involved in the acrosome reaction.
REFERENCE   6  (bases 1 to 1287)
  AUTHORS   Yamatoya,K., Yoshida,K., Ito,C., Maekawa,M., Yanagida,M.,
            Takamori,K., Ogawa,H., Araki,Y., Miyado,K., Toyama,Y. and
            Toshimori,K.
  TITLE     Equatorin: identification and characterization of the epitope of
            the MN9 antibody in the mouse
  JOURNAL   Biol Reprod 81 (5), 889-897 (2009)
   PUBMED   19605790
REFERENCE   7  (bases 1 to 1287)
  AUTHORS   Turner,L.M., Chuong,E.B. and Hoekstra,H.E.
  TITLE     Comparative analysis of testis protein evolution in rodents
  JOURNAL   Genetics 179 (4), 2075-2089 (2008)
   PUBMED   18689890
REFERENCE   8  (bases 1 to 1287)
  AUTHORS   Li,Y.C., Hu,X.Q., Zhang,K.Y., Guo,J., Hu,Z.Y., Tao,S.X., Xiao,L.J.,
            Wang,Q.Z., Han,C.S. and Liu,Y.X.
  TITLE     Afaf, a novel vesicle membrane protein, is related to acrosome
            formation in murine testis
  JOURNAL   FEBS Lett 580 (17), 4266-4273 (2006)
   PUBMED   16831425
  REMARK    GeneRIF: Afaf expressed abundantly in the round spermatids,
            localized in the inner and outer membrane of forming acrosome, and
            declined in the maturing acrosomes.
REFERENCE   9  (bases 1 to 1287)
  AUTHORS   Toshimori,K., Saxena,D.K., Tanii,I. and Yoshinaga,K.
  TITLE     An MN9 antigenic molecule, equatorin, is required for successful
            sperm-oocyte fusion in mice
  JOURNAL   Biol Reprod 59 (1), 22-29 (1998)
   PUBMED   9674989
REFERENCE   10 (bases 1 to 1287)
  AUTHORS   Toshimori,K., Tanii,I., Araki,S. and Oura,C.
  TITLE     Characterization of the antigen recognized by a monoclonal antibody
            MN9: unique transport pathway to the equatorial segment of sperm
            head during spermiogenesis
  JOURNAL   Cell Tissue Res 270 (3), 459-468 (1992)
   PUBMED   1486600
  REMARK    GeneRIF: MN9, a monoclonal antibody raised against mouse
            spermatozoa, specifically recognizes the equatorial segment of
            sperm head in several mammalian species, including humans. The
            immunoreactive bands were 38 kDa and 48 kDa in mouse, and 48 kDa in
            rat.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB572592.1, AK006446.1, AK016738.1, BE632290.1, AK016313.1 and
            BB018628.1.
            
            On Mar 15, 2014 this sequence version replaced NM_027089.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089517.1, EU828781.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                BB572592.1         2-50
            50-730              AK006446.1         1-681
            731-768             AK016738.1         702-739
            769-806             BE632290.1         288-325
            807-929             AK006446.1         758-880
            930-1171            AK016738.1         778-1019
            1172-1285           AK016313.1         1016-1129
            1286-1287           BB018628.1         236-237
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 43.34 cM"
     gene            1..1287
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /note="equatorin, sperm acrosome associated"
                     /db_xref="GeneID:67753"
                     /db_xref="MGI:MGI:1915003"
     exon            1..167
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..1105
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; acrosome formation associated factor;
                     sperm-specific single transmembrane domain protein; MN9
                     antigen"
                     /codon_start=1
                     /product="equatorin isoform 1 precursor"
                     /protein_id="NP_081365.2"
                     /db_xref="CCDS:CCDS18362.1"
                     /db_xref="GeneID:67753"
                     /db_xref="MGI:MGI:1915003"
                     /translation="MDFILLIILSGVFLPDIISLQPIVGQEPGVTLSDEEQYYADEEN
                     NTDGNSVALHKLEENEMDTPANEKTGNYYKDIKQYVFTTPNIKGSEVSVTATTNLEFA
                     VKKNYKASKPTASGEEEKPSESSRKTSTPNIPAFWTILSKAVNETAVSMDDKDQFFQP
                     IPASDLNATNEDKLSELEEIKLKLMLGISLMTLVLLIPLLIFCFATLYKLRHLRDKSY
                     ESQYSINPELATLSYFHPTEGVSDTSFSKSADSNSYWVHNSSEMRRSRTRRSKSKPMD
                     FSAGSNQTVLTDESSFLPPEETRFLLPEEPGKELIVERGPMQAMNEIDAQLLLNKEGS
                     PSN"
     exon            168..326
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     exon            327..410
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     exon            411..473
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     exon            474..518
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     exon            519..578
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     exon            579..732
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     exon            733..1287
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1243..1248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /note="hexamer: AATAAA"
     polyA_site      1262
                     /gene="Eqtn"
                     /gene_synonym="1700028B15Rik; 4930579C15Rik; Afaf; Eqtl;
                     Eqts"
                     /note="major polyA site"
ORIGIN      
        1 aatgtcctat tgagacttag gacctgctgg gccacaagaa gaaagtcctg aggagacagt
       61 agattgctgg ataaatcaca ccaatttccc aatggatttt atactgttga tcattttatc
      121 tggggttttc ctcccagaca ttatcagtct acagcccatt gtgggacagg agcctggtgt
      181 gacactttca gatgaagaac agtattatgc agatgaagaa aataatacag atgggaattc
      241 tgttgcttta cataaattgg aagagaatga aatggacact cctgccaatg agaaaactgg
      301 caattattat aaagacataa aacaatatgt gttcaccaca ccaaacataa aaggttctga
      361 agtatctgtg actgccacaa ctaacctgga atttgctgtg aagaagaact ataaagccag
      421 caaaccaact gccagtggag aagaagaaaa acctagtgaa tcctctcgta aaacctcaac
      481 ccccaatata cctgcatttt ggacaatatt atctaaagct gtaaatgaaa cagcagtgag
      541 catggatgat aaagatcaat tttttcaacc aattccagcc tcagatttga atgctaccaa
      601 tgaagacaaa ctgtcagagc tcgaggaaat caagctgaag ttaatgctgg ggatctcgct
      661 gatgacactg gttctgctaa tccccctctt gatattctgc tttgccacac tgtacaaact
      721 gagacaccta cgtgacaaaa gttatgagag tcaatactcc atcaacccag agctggcaac
      781 tctatcttac ttccatccaa cagaaggtgt gtcagataca tctttttcga aaagtgcaga
      841 cagcaactct tattgggtcc acaattcttc agagatgagg cgatcaagga caagaaggtc
      901 aaaatctaaa cctatggatt tttctgcagg ctccaatcaa acagtcttaa ccgacgagtc
      961 aagcttcctc cctcctgagg agacaagatt cctccttcct gaggagccgg gtaaggagct
     1021 catcgttgag agaggcccta tgcaggcaat gaatgagatt gatgcccagc tgttgttgaa
     1081 caaggaagga tcaccgagta attaactata agtatttggt tataaaaaca gatctttgtg
     1141 tgtcacataa atgttctcat tttgatactg cacatttcag agaaatatat ctacatttct
     1201 acgtcctata tctacattgt ctgacatcct aaggacatat tcaataaaaa ctatcacagt
     1261 tatttttata agaattattt aaatgaa
//
