LOCUS       NM_007946               2691 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus eosinophil peroxidase (Epx), mRNA.
ACCESSION   NM_007946
VERSION     NM_007946.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Li,Y., Liu,S., Zhou,K., Wang,Y., Chen,Y., Hu,W., Li,S., Li,H.,
            Wang,Y., Wang,Q., He,D. and Xu,H.
  TITLE     Neuromedin U programs eosinophils to promote mucosal immunity of
            the small intestine
  JOURNAL   Science 381 (6663), 1189-1196 (2023)
   PUBMED   37708282
REFERENCE   3  (bases 1 to 2691)
  AUTHORS   Fernandes,S., Srivastava,N., Pedicone,C., Sudan,R., Luke,E.A.,
            Dungan,O.M., Pacherille,A., Meyer,S.T., Dormann,S., Schurmans,S.,
            Chambers,B.J., Chisholm,J.D. and Kerr,W.G.
  TITLE     Obesity control by SHIP inhibition requires pan-paralog inhibition
            and an intact eosinophil compartment
  JOURNAL   iScience 26 (2), 106071 (2023)
   PUBMED   36818285
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2691)
  AUTHORS   Ahrends,T., Aydin,B., Matheis,F., Classon,C.H., Marchildon,F.,
            Furtado,G.C., Lira,S.A. and Mucida,D.
  TITLE     Enteric pathogens induce tissue tolerance and prevent neuronal loss
            from subsequent infections
  JOURNAL   Cell 184 (23), 5715-5727 (2021)
   PUBMED   34717799
REFERENCE   5  (bases 1 to 2691)
  AUTHORS   Lu,Y., Huang,Y., Li,J., Huang,J., Zhang,L., Feng,J., Li,J., Xia,Q.,
            Zhao,Q., Huang,L., Jiang,S. and Su,S.
  TITLE     Eosinophil extracellular traps drive asthma progression through
            neuro-immune signals
  JOURNAL   Nat Cell Biol 23 (10), 1060-1072 (2021)
   PUBMED   34616019
REFERENCE   6  (bases 1 to 2691)
  AUTHORS   Seykora,J.T., Mei,L., Dotto,G.P. and Stein,P.L.
  TITLE     'Srcasm: a novel Src activating and signaling molecule
  JOURNAL   J Biol Chem 277 (4), 2812-2822 (2002)
   PUBMED   11711534
REFERENCE   7  (bases 1 to 2691)
  AUTHORS   Denzler,K.L., Borchers,M.T., Crosby,J.R., Cieslewicz,G.,
            Hines,E.M., Justice,J.P., Cormier,S.A., Lindenberger,K.A., Song,W.,
            Wu,W., Hazen,S.L., Gleich,G.J., Lee,J.J. and Lee,N.A.
  TITLE     Extensive eosinophil degranulation and peroxidase-mediated
            oxidation of airway proteins do not occur in a mouse
            ovalbumin-challenge model of pulmonary inflammation
  JOURNAL   J Immunol 167 (3), 1672-1682 (2001)
   PUBMED   11466391
  REMARK    GeneRIF: The activities of EPO, an eosinophilic secondary granule
            protein, do not affect the development of allergic pulmonary
            pathologies in the mouse lung at levels comparable to those
            observed in humans with asthma.
REFERENCE   8  (bases 1 to 2691)
  AUTHORS   Macias,M.P., Welch,K.C., Denzler,K.L., Larson,K.A., Lee,N.A. and
            Lee,J.J.
  TITLE     Identification of a new murine eosinophil major basic protein
            (mMBP) gene: cloning and characterization of mMBP-2
  JOURNAL   J Leukoc Biol 67 (4), 567-576 (2000)
   PUBMED   10770291
REFERENCE   9  (bases 1 to 2691)
  AUTHORS   Denzler,K.L., Levin,W.J., Lee,J.J. and Lee,N.A.
  TITLE     The murine eosinophil peroxidase gene (Epx) maps to chromosome 11
  JOURNAL   Mamm Genome 8 (5), 381-382 (1997)
   PUBMED   9107693
REFERENCE   10 (bases 1 to 2691)
  AUTHORS   Horton,M.A., Larson,K.A., Lee,J.J. and Lee,N.A.
  TITLE     Cloning of the murine eosinophil peroxidase gene (mEPO):
            characterization of a conserved subgroup of mammalian hematopoietic
            peroxidases
  JOURNAL   J Leukoc Biol 60 (2), 285-294 (1996)
   PUBMED   8773591
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB843302.1, D78353.1, L77979.1
            and BB137038.1.
            
            [WARNING] On Jun 6, 2024 this sequence was replaced by NM_007946.3.
            
            On Apr 27, 2007 this sequence version replaced NM_007946.1.
            
            Summary: This gene encodes a member of the peroxidase superfamily
            of enzymes that is produced by eosinophils and plays a critical
            role in eliminating tissue-invasive parasites. The encoded
            preproprotein undergoes proteolytic processing to generate a
            heterodimeric enzyme that forms a predominant component of the
            intracellular granules of eosinophils. Mice lacking the encoded
            protein exhibit resistance to ulcerative colitis induced by dextran
            sulfate. [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052881.1, D78353.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 BB843302.1         1-5
            6-926               D78353.1           12-932
            927-1245            L77979.1           911-1229
            1246-1662           D78353.1           1252-1668
            1663-2682           L77979.1           1647-2666
            2683-2691           BB137038.1         267-275
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 52.25 cM"
     gene            1..2691
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /note="eosinophil peroxidase"
                     /db_xref="GeneID:13861"
                     /db_xref="MGI:MGI:107569"
     exon            1..201
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..2273
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /EC_number="1.11.1.7"
                     /codon_start=1
                     /product="eosinophil peroxidase preproprotein"
                     /protein_id="NP_031972.2"
                     /db_xref="CCDS:CCDS25220.1"
                     /db_xref="GeneID:13861"
                     /db_xref="MGI:MGI:107569"
                     /translation="MMQQLLALVGALATLILTQHAEGTAPASPSPVEISVLRDCIAEA
                     KLLVDTAYNHTQKSIMQRLRSGSASPMDLLAYFKQPVAATRRVVQAADYMHVALGLLE
                     ERLQPRGSRPFNATDVLTEPQLRLLSQASGCALQDQAERCSNKYRTITGRCNNKKHPW
                     LGASNQALARWLPAEYEDHRSLPFGWTPGKRRNGFLLPLVRDVSNQIVRFPSKKLTSD
                     RGRALMFMQWGQFIDHDLDFSPESPARVAFSMGVDCEKTCAQLPPCFPIKIPRNDPRI
                     KNQRDCIPFFRSAPACPQNRNKVRNQINALTSFVDASMVYGSEVTLALRLRNRTNFLG
                     LLATNQRFQDNGRALLPFDNLHEDPCLLTNRSARIPCFLAGDTRSSETPKLTALHTLF
                     VREHNRLAAELRRLNPHWSGDKLYNEARKIVGAMVQIITYRDFLPLVLGRARIRRTLG
                     PYRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYRASAPNSHVPLSSVFFASW
                     RIIHEGGIDPILRGLMATPAKLNRQDSMLVDELRDKLFQQVRRIGLDLAALNMQRSRD
                     HGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLRLYKTPDNIDIWVGAIAEPL
                     LPGARVGPLLACLFENQFRRARDGDRFWWQKWGVFTKRQRKALRRISLSRIVCDNTGI
                     TTVSRDIFRANIYPQGFVSCSRIPKLNLSAWRGK"
     exon            202..295
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            296..471
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            472..589
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            590..719
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            720..926
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1245
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1406
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1662
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1833
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2071
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2284
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2691
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2670..2675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /note="hexamer: AATAAA"
     polyA_site      2689
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /note="major polyA site"
ORIGIN      
        1 gagaagagta agaagaaaga aaccatcaca ggacctctaa gggagagagg aattgtacac
       61 tcacctccgt ggcatctgtg ggcattgctt gcctgcttag ctgtagtggg ggttcagctt
      121 gcatgatgca gcagcttctg gccctggtag gggccttagc cacactcatc ctcacccaac
      181 acgctgaagg cactgcccca gcttccccca gtccagtgga gatttcagtc cttcgagact
      241 gcatagcaga ggctaagctg ctggtggaca ctgcctacaa tcacacacag aaaagcatca
      301 tgcaacgtct tcgcagtggc tcggccagcc ccatggacct cctggcctac ttcaaacagc
      361 cagtagcagc caccagaaga gtggttcagg ccgctgatta tatgcatgtg gccttagggc
      421 tgctagaaga gaggctgcag ccacggggat ctagaccctt caatgccact gatgtgctca
      481 cagaaccaca gctgcgcctg ctgtcccagg ccagtggctg tgctctccag gaccaggctg
      541 agaggtgcag caacaagtac cgaaccataa cagggagatg caacaacaag aagcatccct
      601 ggctgggagc ctccaaccag gccctggccc gctggctgcc agctgagtat gaagaccaca
      661 ggtctctccc ctttggctgg acccctggaa agaggcgtaa tggcttcctg cttcctcttg
      721 tccgggatgt ctccaatcag attgtgcgct tccccagcaa gaaactgacc tcagaccgcg
      781 gccgggccct tatgttcatg cagtggggcc agttcatcga ccatgacctg gacttctccc
      841 cggagtcccc agccagagtg gccttcagca tgggtgtgga ctgtgagaag acctgtgccc
      901 agctgccccc ctgcttcccc atcaagatcc cacgcaatga tccccgcatc aagaaccagc
      961 gcgactgcat ccccttcttc cgctctgccc cggcgtgccc ccaaaacagg aacaaagtcc
     1021 gcaaccagat caacgcactt acatccttcg tggacgccag catggtgtat ggcagcgagg
     1081 tgaccctcgc cctgcggctc cgtaatagga ccaacttcct ggggctgctg gccacgaacc
     1141 agcgattcca ggacaacggt cgagccctac tgccttttga caacctgcat gaggacccct
     1201 gtctcctgac taaccgctct gcacgcatcc cctgcttcct ggcaggtgac actcggtcaa
     1261 gtgaaacccc caaactgacg gccttgcaca cgctgtttgt gagagaacac aatcgcctag
     1321 ccgctgagct gaggcgcctg aatccccact ggagtggaga caagctgtac aacgaagccc
     1381 ggaagatcgt gggagccatg gtccagatca tcacctaccg agacttcctg cctctggttc
     1441 tgggcagggc ccggatcaga agaactttgg gtccctacag agggtattgc tccaatgtgg
     1501 acccccgtgt agccaatgtt ttcaccctgg ccttccgctt tggccacacc atgcttcagc
     1561 ccttcatgtt ccgcctggat agccagtatc gggcctcggc acccaactcc cacgtcccgc
     1621 ttagctctgt attctttgcc agctggcgaa tcatacatga aggtggcatc gaccctatcc
     1681 tccgaggtct catggccacc ccagctaagc tgaaccggca ggattccatg ctggtggatg
     1741 agctccgcga caagctgttt cagcaagtga gaaggatcgg gctggatctg gcagctctga
     1801 acatgcagcg cagccgggac cacggccttc caggatacaa tgcctggaga cgcttctgtg
     1861 ggctttccca gccccgaaac ttggcccagc ttagtcgggt gctaaaaaat caggatttgg
     1921 caaggaagtt tctgagactg tacaagacgc ccgacaacat tgacatctgg gtcggagcca
     1981 ttgcggagcc tctcctgccg ggggcccgag tggggccgct tctggcttgt ctttttgaga
     2041 accagttcag gagagccaga gatggagaca gattctggtg gcagaaatgg ggagttttca
     2101 ccaaaagaca acgcaaagcc ctgaggcgaa tctctttatc tcggattgta tgcgacaata
     2161 ctggcatcac cacggtttcg agggacatct tcagggctaa catctaccca cagggttttg
     2221 tgagctgtag ccgtattccc aagttgaacc tatcagcctg gcgaggcaaa tgagggttct
     2281 gcaggattct acgccaaaac cccaagcctt gaagatgagg gtaacgggga gatcatgagg
     2341 ctgctttctg ccctgcgaac cactgtttcc tgctagagct tttgctggtt aaactcagag
     2401 aaggcttttt aggttgagag cgtcaagggt gctatggcaa caggcccact tacctcggga
     2461 acctcttaga tagcacatta tgaatcagca ccaggtgtaa gagcctatga gcagagccat
     2521 gaggctttga ggtcccagca agacactgcg ggagggcttc cagcacccct gccctcagca
     2581 tgacaggaag agctcacaat cctgcctctt agccatccac catcttcctt gggacctgaa
     2641 ggaacagcag cactctctct acttctacca ataaaacaat gcaagagcag g
//
