LOCUS       NP_031972                716 aa            linear   ROD 13-MAY-2025
DEFINITION  eosinophil peroxidase isoform 1 precursor [Mus musculus].
ACCESSION   NP_031972
VERSION     NP_031972.2
DBSOURCE    REFSEQ: accession NM_007946.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 716)
  AUTHORS   Radhouani,M., Farhat,A., Hakobyan,A., Zahalka,S., Pimenov,L.,
            Fokina,A., Hladik,A., Lakovits,K., Brosamlen,J., Dvorak,V.,
            Nunes,N., Zech,A., Idzko,M., Krausgruber,T., Kohl,J., Uluckan,O.,
            Kovarik,J., Hoehlig,K., Vater,A., Eckhard,M., Sombke,A.,
            Fortelny,N., Menche,J., Knapp,S. and Starkl,P.
  TITLE     Eosinophil innate immune memory after bacterial skin infection
            promotes allergic lung inflammation
  JOURNAL   Sci Immunol 10 (106), eadp6231 (2025)
   PUBMED   40184438
REFERENCE   2  (residues 1 to 716)
  AUTHORS   Kim,H.J., Jang,J., Na,K., Lee,E.H., Gu,H.J., Lim,Y.H., Joo,S.A.,
            Baek,S.E., Roh,J.Y., Maeng,H.J., Kim,Y.H., Lee,Y.J., Oh,B.C. and
            Jung,Y.
  TITLE     TLR7-dependent eosinophil degranulation links psoriatic skin
            inflammation to small intestinal inflammatory changes in mice
  JOURNAL   Exp Mol Med 56 (5), 1164-1177 (2024)
   PUBMED   38689088
REFERENCE   3  (residues 1 to 716)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (residues 1 to 716)
  AUTHORS   First,N.J., Parrish,K.M., Martinez-Perez,A., Gonzalez-Fernandez,A.,
            Bharrhan,S., Woolard,M., McLachlan,J.B., Scott,R.S., Wang,J. and
            Gestal,M.C.
  TITLE     Bordetella spp. block eosinophil recruitment to suppress the
            generation of early mucosal protection
  JOURNAL   Cell Rep 42 (11), 113294 (2023)
   PUBMED   37883230
REFERENCE   5  (residues 1 to 716)
  AUTHORS   Li,Y., Liu,S., Zhou,K., Wang,Y., Chen,Y., Hu,W., Li,S., Li,H.,
            Wang,Y., Wang,Q., He,D. and Xu,H.
  TITLE     Neuromedin U programs eosinophils to promote mucosal immunity of
            the small intestine
  JOURNAL   Science 381 (6663), 1189-1196 (2023)
   PUBMED   37708282
REFERENCE   6  (residues 1 to 716)
  AUTHORS   Seykora,J.T., Mei,L., Dotto,G.P. and Stein,P.L.
  TITLE     'Srcasm: a novel Src activating and signaling molecule
  JOURNAL   J Biol Chem 277 (4), 2812-2822 (2002)
   PUBMED   11711534
REFERENCE   7  (residues 1 to 716)
  AUTHORS   Denzler,K.L., Borchers,M.T., Crosby,J.R., Cieslewicz,G.,
            Hines,E.M., Justice,J.P., Cormier,S.A., Lindenberger,K.A., Song,W.,
            Wu,W., Hazen,S.L., Gleich,G.J., Lee,J.J. and Lee,N.A.
  TITLE     Extensive eosinophil degranulation and peroxidase-mediated
            oxidation of airway proteins do not occur in a mouse
            ovalbumin-challenge model of pulmonary inflammation
  JOURNAL   J Immunol 167 (3), 1672-1682 (2001)
   PUBMED   11466391
  REMARK    GeneRIF: The activities of EPO, an eosinophilic secondary granule
            protein, do not affect the development of allergic pulmonary
            pathologies in the mouse lung at levels comparable to those
            observed in humans with asthma.
REFERENCE   8  (residues 1 to 716)
  AUTHORS   Macias,M.P., Welch,K.C., Denzler,K.L., Larson,K.A., Lee,N.A. and
            Lee,J.J.
  TITLE     Identification of a new murine eosinophil major basic protein
            (mMBP) gene: cloning and characterization of mMBP-2
  JOURNAL   J Leukoc Biol 67 (4), 567-576 (2000)
   PUBMED   10770291
REFERENCE   9  (residues 1 to 716)
  AUTHORS   Denzler,K.L., Levin,W.J., Lee,J.J. and Lee,N.A.
  TITLE     The murine eosinophil peroxidase gene (Epx) maps to chromosome 11
  JOURNAL   Mamm Genome 8 (5), 381-382 (1997)
   PUBMED   9107693
REFERENCE   10 (residues 1 to 716)
  AUTHORS   Horton,M.A., Larson,K.A., Lee,J.J. and Lee,N.A.
  TITLE     Cloning of the murine eosinophil peroxidase gene (mEPO):
            characterization of a conserved subgroup of mammalian hematopoietic
            peroxidases
  JOURNAL   J Leukoc Biol 60 (2), 285-294 (1996)
   PUBMED   8773591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL606805.21.
            
            On Apr 27, 2007 this sequence version replaced NP_031972.1.
            
            Summary: This gene encodes a member of the peroxidase superfamily
            of enzymes that is produced by eosinophils and plays a critical
            role in eliminating tissue-invasive parasites. The encoded
            preproprotein undergoes proteolytic processing to generate a
            heterodimeric enzyme that forms a predominant component of the
            intracellular granules of eosinophils. Mice lacking the encoded
            protein exhibit resistance to ulcerative colitis induced by dextran
            sulfate. [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052881.1, D78353.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 52.25 cM"
     Protein         1..716
                     /product="eosinophil peroxidase isoform 1 precursor"
                     /EC_number="1.11.1.7"
                     /calculated_mol_wt=79498
     sig_peptide     1..18
                     /note="/evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P49290.2)"
                     /calculated_mol_wt=1900
     Site            53
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P49290.2)"
     Site            114
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P49290.2)"
     mat_peptide     141..251
                     /product="Eosinophil peroxidase light chain.
                     /id=PRO_0000023643"
                     /note="propagated from UniProtKB/Swiss-Prot (P49290.2)"
                     /calculated_mol_wt=12836
     mat_peptide     252..716
                     /product="Eosinophil peroxidase heavy chain.
                     /id=PRO_0000023644"
                     /note="propagated from UniProtKB/Swiss-Prot (P49290.2)"
                     /calculated_mol_wt=53413
     Region          288..700
                     /region_name="myeloperoxidase_like"
                     /note="Myeloperoxidases, eosinophil peroxidases, and
                     lactoperoxidases; cd09824"
                     /db_xref="CDD:188656"
     Site            order(303,574,577)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188656"
     Site            order(307,311,313)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:188656"
     Site            order(328,364,456)
                     /site_type="other"
                     /note="putative glycosylation site [posttranslational
                     modification]"
                     /db_xref="CDD:188656"
     Site            328
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P49290.2)"
     Site            364
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P49290.2)"
     Site            order(378,381,468,471..472,475,478,504,556,563)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:188656"
     Site            378
                     /site_type="other"
                     /note="Transition state stabilizer.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00298; propagated
                     from UniProtKB/Swiss-Prot (P49290.2)"
     Site            order(432,435..436,440,467,471,473,630,634,638..639,
                     642..643,646)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:188656"
     Site            709
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P49290.2)"
     CDS             1..716
                     /gene="Epx"
                     /gene_synonym="EPO"
                     /coded_by="NM_007946.3:119..2269"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS25220.1"
                     /db_xref="GeneID:13861"
                     /db_xref="MGI:MGI:107569"
ORIGIN      
        1 mmqqllalvg alatliltqh aegtapasps pveisvlrdc iaeakllvdt aynhtqksim
       61 qrlrsgsasp mdllayfkqp vaatrrvvqa adymhvalgl leerlqprgs rpfnatdvlt
      121 epqlrllsqa sgcalqdqae rcsnkyrtit grcnnkkhpw lgasnqalar wlpaeyedhr
      181 slpfgwtpgk rrngfllplv rdvsnqivrf pskkltsdrg ralmfmqwgq fidhdldfsp
      241 esparvafsm gvdcektcaq lppcfpikip rndpriknqr dcipffrsap acpqnrnkvr
      301 nqinaltsfv dasmvygsev tlalrlrnrt nflgllatnq rfqdngrall pfdnlhedpc
      361 lltnrsarip cflagdtrss etpkltalht lfvrehnrla aelrrlnphw sgdklynear
      421 kivgamvqii tyrdflplvl grarirrtlg pyrgycsnvd prvanvftla frfghtmlqp
      481 fmfrldsqyr asapnshvpl ssvffaswri iheggidpil rglmatpakl nrqdsmlvde
      541 lrdklfqqvr rigldlaaln mqrsrdhglp gynawrrfcg lsqprnlaql srvlknqdla
      601 rkflrlyktp dnidiwvgai aepllpgarv gpllaclfen qfrrardgdr fwwqkwgvft
      661 krqrkalrri slsrivcdnt gittvsrdif raniypqgfv scsripklnl sawrgk
//
