LOCUS       NM_133191               3090 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus EPS8-like 2 (Eps8l2), mRNA.
ACCESSION   NM_133191
VERSION     NM_133191.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Braitsch,C.M., Azizoglu,D.B., Htike,Y., Barlow,H.R., Schnell,U.,
            Chaney,C.P., Carroll,T.J., Stanger,B.Z. and Cleaver,O.
  TITLE     LATS1/2 suppress NFkappaB and aberrant EMT initiation to permit
            pancreatic progenitor differentiation
  JOURNAL   PLoS Biol 17 (7), e3000382 (2019)
   PUBMED   31323030
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3090)
  AUTHORS   Giese,A.P.J., Tang,Y.Q., Sinha,G.P., Bowl,M.R., Goldring,A.C.,
            Parker,A., Freeman,M.J., Brown,S.D.M., Riazuddin,S., Fettiplace,R.,
            Schafer,W.R., Frolenkov,G.I. and Ahmed,Z.M.
  TITLE     CIB2 interacts with TMC1 and TMC2 and is essential for
            mechanotransduction in auditory hair cells
  JOURNAL   Nat Commun 8 (1), 43 (2017)
   PUBMED   28663585
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3090)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 3090)
  AUTHORS   Furness,D.N., Johnson,S.L., Manor,U., Ruttiger,L., Tocchetti,A.,
            Offenhauser,N., Olt,J., Goodyear,R.J., Vijayakumar,S., Dai,Y.,
            Hackney,C.M., Franz,C., Di Fiore,P.P., Masetto,S., Jones,S.M.,
            Knipper,M., Holley,M.C., Richardson,G.P., Kachar,B. and Marcotti,W.
  TITLE     Progressive hearing loss and gradual deterioration of sensory hair
            bundles in the ears of mice lacking the actin-binding protein
            Eps8L2
  JOURNAL   Proc Natl Acad Sci U S A 110 (34), 13898-13903 (2013)
   PUBMED   23918390
  REMARK    GeneRIF: Eps8L2 is required for the long-term maintenance of the
            staircase structure and mechanosensory function of auditory hair
            bundles.
REFERENCE   6  (bases 1 to 3090)
  AUTHORS   Bell,S.M., Zhang,L., Xu,Y., Besnard,V., Wert,S.E., Shroyer,N. and
            Whitsett,J.A.
  TITLE     Kruppel-like factor 5 controls villus formation and initiation of
            cytodifferentiation in the embryonic intestinal epithelium
  JOURNAL   Dev Biol 375 (2), 128-139 (2013)
   PUBMED   23266329
REFERENCE   7  (bases 1 to 3090)
  AUTHORS   Disanza,A., Carlier,M.F., Stradal,T.E., Didry,D., Frittoli,E.,
            Confalonieri,S., Croce,A., Wehland,J., Di Fiore,P.P. and Scita,G.
  TITLE     Eps8 controls actin-based motility by capping the barbed ends of
            actin filaments
  JOURNAL   Nat Cell Biol 6 (12), 1180-1188 (2004)
   PUBMED   15558031
REFERENCE   8  (bases 1 to 3090)
  AUTHORS   Offenhauser,N., Borgonovo,A., Disanza,A., Romano,P., Ponzanelli,I.,
            Iannolo,G., Di Fiore,P.P. and Scita,G.
  TITLE     The eps8 family of proteins links growth factor stimulation to
            actin reorganization generating functional redundancy in the
            Ras/Rac pathway
  JOURNAL   Mol Biol Cell 15 (1), 91-98 (2004)
   PUBMED   14565974
  REMARK    Erratum:[Mol Biol Cell. 2019 Sep 1;30(19):2535. doi:
            10.1091/mbc.E03-06-0427-corr.. PMID: 31465253]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163434.5.
            
            On Jan 12, 2022 this sequence version replaced NM_133191.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005492.1, SRR7974084.22460.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                AC163434.5         135212-135289
            79-160              AC163434.5         138124-138205
            161-216             AC163434.5         138286-138341
            217-281             AC163434.5         139157-139221
            282-443             AC163434.5         151177-151338
            444-593             AC163434.5         151876-152025
            594-673             AC163434.5         152101-152180
            674-822             AC163434.5         152323-152471
            823-893             AC163434.5         152556-152626
            894-1020            AC163434.5         152768-152894
            1021-1109           AC163434.5         153278-153366
            1110-1184           AC163434.5         153473-153547
            1185-1333           AC163434.5         153774-153922
            1334-1466           AC163434.5         154017-154149
            1467-1579           AC163434.5         154436-154548
            1580-1685           AC163434.5         156553-156658
            1686-1805           AC163434.5         157233-157352
            1806-1878           AC163434.5         157612-157684
            1879-2059           AC163434.5         157811-157991
            2060-2192           AC163434.5         158079-158211
            2193-3090           AC163434.5         158354-159251
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 86.66 cM"
     gene            1..3090
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /note="EPS8-like 2"
                     /db_xref="GeneID:98845"
                     /db_xref="MGI:MGI:2138828"
     exon            1..78
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            79..160
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..2306
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /note="epidermal growth factor receptor pathway substrate
                     8-like protein 2; EPS8-like protein 2; epidermal growth
                     factor receptor pathway substrate 8-related protein 2;
                     EPS8 related protein 2"
                     /codon_start=1
                     /product="epidermal growth factor receptor kinase
                     substrate 8-like protein 2"
                     /protein_id="NP_573454.2"
                     /db_xref="CCDS:CCDS22009.1"
                     /db_xref="GeneID:98845"
                     /db_xref="MGI:MGI:2138828"
                     /translation="MSQSASMSCCPGAANGSLGRSDGVPRMSAKDLFEQRKKYSNSNV
                     IMHETSQYHVQHLATFIMDKSEAIASVDDAIRKLVQLSSKEKVWAQEVLLQVNDKSLR
                     LLDVESQEELENFPLPTVQHSQTVLNQLRYPSVLLLVCQDSDQNKPDIHFFHCDEVEA
                     ELVQEDIESALADYRLGKKMRPQTLKGHQEKIRQRQSILPPPQSPAPIPFQRQPGDSP
                     QAKNRVGLPLPVPFSEPGYRRRESQDEEPRAVLAQRIEKETQILNCTLDDIEWFVARL
                     QKAAEAFKQLNQRKKGKKKNKKGPAEGVLTLRARPPSEGEFVDCFQKTKLAINLLAKL
                     QKHIQNPSAAELVHFLFGPLDLIINTCGSPDIARSVSSPLLSTDAVSFLRGHLVPKEM
                     TLWESLGETWMRPRSEWPREPQVPLYVPKFRSGWEPPLDVLQEAPWEVEGLASVPSDQ
                     LTPKNRLSVRHSPKHSLSSESQAPEDIAPPGSSPHANRGYQPTPAMTKYVKILYDFTA
                     RNANELSVLKDEVLEVLEDGRQWWKLRNRSGQAGYVPCNILAEARQEDVGAPLEQSGQ
                     KYWGPASPTHKLPPIFAGNKEELIHHMDEVNDELMKKISHIKTQPQRNFRVERSQPVH
                     LPLTFESGPDEVRAWLEAKAFSARIVENLGILTGPQLFSLNKEELKKVCGEEGSRVYS
                     QLTVQKAFLEKQQSGSELEKLMSKIRRAEDSYTSQHTSPESEGAPHL"
     exon            161..216
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            217..281
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            282..443
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            444..593
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            594..673
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            674..822
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            823..893
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1020
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1109
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1184
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1333
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1466
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1579
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1685
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1805
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1878
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2059
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2192
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..3090
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3067..3072
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /note="hexamer: AATAAA"
     polyA_site      3090
                     /gene="Eps8l2"
                     /gene_synonym="Eps8r2"
                     /note="major polyA site"
ORIGIN      
        1 agaggctctg acagacagac ggcggacaag ctgaccgagg ggcctaacca agcacaggcg
       61 ctgagggacg gagcgcaggt ctgccctccc tgacactggc taccactggg aacgtcatga
      121 gccagtcagc atccatgagc tgctgcccag gagctgccaa cggcagtctg ggccggtctg
      181 atggtgtgcc caggatgagc gcaaaggacc tgtttgagca gaggaagaag tactccaact
      241 ccaacgtcat catgcatgag acttcacagt atcacgtgca gcacctggcc acattcatca
      301 tggacaagag tgaggctatt gcctccgtgg atgacgccat ccggaagctg gtacagctga
      361 gctccaagga gaaggtctgg gcccaggaag tcctgctgca ggtgaacgac aagtcactgc
      421 gactgctgga tgttgagtcc caggaagagc tggagaactt tccgctgccc actgtgcagc
      481 acagccagac agtgctaaac cagctgcgct acccctctgt gctgctgctg gtgtgccagg
      541 actcagacca gaacaagccc gacatccact tctttcactg tgacgaggtg gaggcggaac
      601 ttgtgcagga agacattgag agtgctctgg ctgactaccg gctggggaag aagatgagac
      661 cacagaccct aaagggacac caggagaaga tccgccagcg gcagtctatc ttaccccctc
      721 cacagagccc agcccccatt cccttccagc gacagccagg agattcccct caggccaaga
      781 accgtgtggg cctgcccttg ccagtgccat tcagtgaacc aggctatcgc aggagggaat
      841 cccaggacga ggagccacgg gctgtgctgg ctcagagaat agagaaggag acgcaaatcc
      901 ttaactgcac cctggatgat attgaatggt tcgtggccag gctgcagaag gcagctgagg
      961 ctttcaagca gttgaaccag cggaaaaagg ggaagaagaa gaacaagaag gggccagcag
     1021 agggtgtcct tacacttcgg gcacgccccc cctcggaggg agagtttgtt gactgtttcc
     1081 agaaaaccaa gctggccatc aacctgctgg cgaagctgca gaagcacatc caaaacccta
     1141 gtgcagccga gctggtgcac ttcctcttcg ggcctctgga tctgatcatc aacacatgtg
     1201 gcagccccga catagcacgc tctgtctcca gccctttgct gtccactgac gccgtgagct
     1261 tcttacgtgg ccacttggtc cccaaagaga tgacactgtg ggaatcactg ggggagacct
     1321 ggatgaggcc tcgctctgaa tggccccggg agccacaggt gccactctat gtgcccaagt
     1381 tccgcagcgg ctgggaacca cccctggatg tcctacagga ggctccctgg gaggtagaag
     1441 gactggcatc tgtgcccagt gatcagctga cccccaagaa tcgactatct gtacgacatt
     1501 ccccaaagca cagcctctcc tccgagtccc aagccccaga ggacattgca ccaccaggca
     1561 gctccccaca tgctaacagg ggctaccagc caacaccagc catgaccaag tacgtcaaga
     1621 tcctctatga tttcacagcg cgaaacgcca atgagctatc tgtgctcaag gacgaggtcc
     1681 tagaggtgct ggaggacggc cggcagtggt ggaagctacg aaatcgcagt ggccaggcgg
     1741 gctatgtgcc ctgcaacatc ctggctgaag ctcggcagga ggacgtgggt gcccccttgg
     1801 aacagtctgg ccagaaatac tggggtccag ccagccccac ccacaaactg cccccaattt
     1861 tcgcagggaa caaagaagag ctaatccacc acatggacga ggtcaatgac gagctcatga
     1921 aaaagatcag ccacatcaag acacagccgc agcgcaactt ccgcgtggag cgcagccagc
     1981 cagtgcacct gccgcttacc ttcgagtcag gtccggatga ggtccgcgcc tggctggaag
     2041 ccaaggcctt cagtgccagg atcgtggaga acctgggcat cttaactgga ccccagctct
     2101 tctccctcaa caaggaggaa ctgaaaaaag tgtgcgggga agagggcagt cgcgtgtaca
     2161 gccagctcac ggttcagaag gccttcctgg agaaacaaca aagtgggtca gaactggaga
     2221 agctcatgag caagatcagg agggccgagg acagctatac cagccaacac acttcaccag
     2281 agtcagaggg ggcgccgcac ctgtaatggg cccaacccaa atgcatggag tattattttt
     2341 atatgtgtat tattttatac caaggacata gatgggctgg gtgtaactgg cttacctgcc
     2401 ctccaggcag agtcctgtgt atgagcctag ctacacctgt gggtagctct tttaccaccc
     2461 gagccctacc cccaacccaa tactcttctt ccaaaaccaa ggccagcctc agccaaacct
     2521 gtacctggca gtgccagtcc cttctcaacc tgctgccaag gccccaggac tctcccggtg
     2581 ggcaaggcct cttcccctgg tcttcttggt gcacatgaag gtccttgttc cttacaatgc
     2641 tgggccccga ctctgcagct ctcccttgtg ttgcttgttt ctacattcac gttttcctgc
     2701 atccgctgta ccccaattgc aggatgggcc tgagccctca gctgaccagc ctggtgtcct
     2761 tgcctacata tttgcccact cccttcacgc tgctagcatc ccaggaacaa cctggtcagg
     2821 ggctccagga caggaggcct tgggtgacct gctggtatag agtatgctgt attctttctt
     2881 ctttgagacc taagaatgaa ccagacatag gttctgactt caaatgtccc atcccaccca
     2941 aagagcctgt gataacagat gtctctggct atttccacca gcctgttcgc tggtgtctgg
     3001 tgcgtttccc agctctggat ggagccccag acccagagat gagagtgggg actccacagc
     3061 tatgtcaata aaccagatgt ctcctagcaa
//
