LOCUS       NM_007945               4547 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus epidermal growth factor receptor pathway substrate 8
            (Eps8), transcript variant 1, mRNA.
ACCESSION   NM_007945
VERSION     NM_007945.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Dolat,L., Carpenter,V.K., Chen,Y.S., Suzuki,M., Smith,E.P.,
            Kuddar,O. and Valdivia,R.H.
  TITLE     Chlamydia repurposes the actin-binding protein EPS8 to disassemble
            epithelial tight junctions and promote infection
  JOURNAL   Cell Host Microbe 30 (12), 1685-1700 (2022)
   PUBMED   36395759
  REMARK    GeneRIF: Chlamydia repurposes the actin-binding protein EPS8 to
            disassemble epithelial tight junctions and promote infection.
REFERENCE   3  (bases 1 to 4547)
  AUTHORS   Akturk,A., Day,M. and Tarchini,B.
  TITLE     RGS12 polarizes the GPSM2-GNAI complex to organize and elongate
            stereocilia in sensory hair cells
  JOURNAL   Sci Adv 8 (42), eabq2826 (2022)
   PUBMED   36260679
REFERENCE   4  (bases 1 to 4547)
  AUTHORS   Halford,J., Bateschell,M. and Barr-Gillespie,P.G.
  TITLE     Ca2+ entry through mechanotransduction channels localizes BAIAP2L2
            to stereocilia tips
  JOURNAL   Mol Biol Cell 33 (4), br6 (2022)
   PUBMED   35044843
REFERENCE   5  (bases 1 to 4547)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4547)
  AUTHORS   Biesova,Z., Piccoli,C. and Wong,W.T.
  TITLE     Isolation and characterization of e3B1, an eps8 binding protein
            that regulates cell growth
  JOURNAL   Oncogene 14 (2), 233-241 (1997)
   PUBMED   9010225
REFERENCE   7  (bases 1 to 4547)
  AUTHORS   Avantaggiato,V., Torino,A., Wong,W.T., Di Fiore,P.P. and Simeone,A.
  TITLE     Expression of the receptor tyrosine kinase substrate genes eps8 and
            eps15 during mouse development
  JOURNAL   Oncogene 11 (6), 1191-1198 (1995)
   PUBMED   7566980
REFERENCE   8  (bases 1 to 4547)
  AUTHORS   Karlsson,T., Songyang,Z., Landgren,E., Lavergne,C., Di Fiore,P.P.,
            Anafi,M., Pawson,T., Cantley,L.C., Claesson-Welsh,L. and Welsh,M.
  TITLE     Molecular interactions of the Src homology 2 domain protein Shb
            with phosphotyrosine residues, tyrosine kinase receptors and Src
            homology 3 domain proteins
  JOURNAL   Oncogene 10 (8), 1475-1483 (1995)
   PUBMED   7537362
REFERENCE   9  (bases 1 to 4547)
  AUTHORS   Wong,W.T., Carlomagno,F., Druck,T., Barletta,C., Croce,C.M.,
            Huebner,K., Kraus,M.H. and Di Fiore,P.P.
  TITLE     Evolutionary conservation of the EPS8 gene and its mapping to human
            chromosome 12q23-q24
  JOURNAL   Oncogene 9 (10), 3057-3061 (1994)
   PUBMED   8084614
REFERENCE   10 (bases 1 to 4547)
  AUTHORS   Fazioli,F., Minichiello,L., Matoska,V., Castagnino,P., Miki,T.,
            Wong,W.T. and Di Fiore,P.P.
  TITLE     Eps8, a substrate for the epidermal growth factor receptor kinase,
            enhances EGF-dependent mitogenic signals
  JOURNAL   EMBO J 12 (10), 3799-3808 (1993)
   PUBMED   8404850
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166347.2 and AC093120.4.
            
            On Jul 21, 2022 this sequence version replaced NM_007945.3.
            
            Transcript Variant: This variant (1) encodes isoform 1. Variants 1,
            2, 3, and 6 all encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166156.1, SRR7652917.670780.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               AC166347.2         52682-52894
            214-288             AC166347.2         110454-110528
            289-368             AC093120.4         185830-185909       c
            369-445             AC093120.4         184126-184202       c
            446-513             AC093120.4         175033-175100       c
            514-675             AC093120.4         173876-174037       c
            676-825             AC093120.4         173596-173745       c
            826-908             AC093120.4         169338-169420       c
            909-1045            AC093120.4         168661-168797       c
            1046-1119           AC093120.4         165951-166024       c
            1120-1246           AC093120.4         163579-163705       c
            1247-1335           AC093120.4         161211-161299       c
            1336-1410           AC093120.4         160786-160860       c
            1411-1559           AC093120.4         158657-158805       c
            1560-1740           AC093120.4         155590-155770       c
            1741-1874           AC093120.4         151896-152029       c
            1875-1983           AC093120.4         147043-147151       c
            1984-2127           AC093120.4         146090-146233       c
            2128-2350           AC093120.4         136725-136947       c
            2351-2531           AC093120.4         130050-130230       c
            2532-2661           AC093120.4         128660-128789       c
            2662-4547           AC093120.4         123752-125637       c
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 66.78 cM"
     gene            1..4547
                     /gene="Eps8"
                     /note="epidermal growth factor receptor pathway substrate
                     8"
                     /db_xref="GeneID:13860"
                     /db_xref="MGI:MGI:104684"
     exon            1..213
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            214..288
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            289..368
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     CDS             310..2775
                     /gene="Eps8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     epidermal growth factor receptor kinase substrate 8"
                     /codon_start=1
                     /product="epidermal growth factor receptor kinase
                     substrate 8 isoform 1"
                     /protein_id="NP_031971.2"
                     /db_xref="CCDS:CCDS20665.1"
                     /db_xref="GeneID:13860"
                     /db_xref="MGI:MGI:104684"
                     /translation="MNGHMSNRSSGYGVYPSQLNGYGSSPPYSQMDREHSSRTSAKAL
                     YEQRKNYARDSVSSVSDVSQYRVEHLTTFVLDRKDAMITVEDGIRKLKLLDAKGKVWT
                     QDMILQVDDRAVSLIDLESKNELENFPLNTISHCQAVVHACSYDSILALVCKEPTQSK
                     PDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPEALRMIAKADPGIPPPPRAPAP
                     VPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARIDRDVQILNHI
                     LDDIEFFITKLQKAAEAFSELSKRKKSKKSKRKGPGEGVLTLRAKPPPPDEFVDCFQK
                     FKHGFNLLAKLKSHIQNPSASDLVHFLFTPLNMVVQATGGPELASSVLSPLLTKDTVD
                     FLNYTATAEERKLWMSLGDSWVKVRAEWPKEQFIPPYVPRFRNGWEPPMLNFMGAPTE
                     QDMYQLAESVANAEHQRKQDSKRLSTEHSNVSDYPPADGYAYSSSMYHRGPHADHGEA
                     AMPFKSTPNHQVDRNYDAVKTQPKKYAKSKYDFVARNSSELSVMKDDVLEILDDRRQW
                     WKVRNASGDSGFVPNNILDIMRTPESGVGRADPPYTHTIQKQRTEYGLRSADTPSAPS
                     PPPTPAPVPVPLPPSVPAPVSVPKVPANVTRQNSSSSDSGGSIVRDSQRYKQLPVDRR
                     KSQMEEVQDELFQRLTIGRSAAQRKFHVPRQNVPVINITYDSSPEEVKTWLQSKGFNP
                     VTVNSLGVLNGAQLFSLNKDELRSVCPEGARVFNQITVQKAALEDSNGSSELQEIMRR
                     RQEKISAAASDSGVESFDEGSSH"
     exon            369..445
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            446..513
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            514..675
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            676..825
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            826..908
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1045
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1119
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1246
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1335
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1410
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1559
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1740
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1874
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1983
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2127
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2350
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2351..2531
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2661
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..4547
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4072..4077
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: AATAAA"
     regulatory      4096..4101
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: AATAAA"
     polyA_site      4097
                     /gene="Eps8"
                     /note="major polyA site"
     polyA_site      4119
                     /gene="Eps8"
                     /note="major polyA site"
     regulatory      4300..4305
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: GATAAA"
     polyA_site      4321
                     /gene="Eps8"
     regulatory      4524..4529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: AATAAA"
     polyA_site      4547
                     /gene="Eps8"
ORIGIN      
        1 acacacggcc cgggcggcgg gcgaagcggg ctcccggggc gctgggcgca gggcgcaggg
       61 cgcagggcgc ggggcaagcc ccagcggcgt gtctgcagcg gggcgcggcg ggcgctccag
      121 ctccgggatc tttctccctc cgtcacctcc ctcgcgtcta gggaggtcgt ggcactccct
      181 gaggagcgcg gctgctcgga gggcggatcc taggctgtct atatgtgata cagcacgcac
      241 tgcattgaag tggcagcaca agaatcggag attattttca tctctgagaa cagaggcgtg
      301 agagccggca tgaatggtca tatgtctaac cgctccagtg ggtatggagt ctacccttct
      361 caactgaatg gttacggatc ttcaccaccc tattcccaga tggacagaga acacagctca
      421 agaacaagtg caaaggccct ttatgaacaa aggaagaact atgcccgaga cagtgtcagc
      481 agtgtgtcgg acgtgtccca gtaccgcgtg gaacacttga ccaccttcgt gctggatcgg
      541 aaagatgcaa tgatcactgt cgaggacgga ataagaaagc tgaagttgct ggatgccaag
      601 ggcaaagtgt ggactcaaga tatgattctc caagtggatg accgagctgt gagcctgatt
      661 gacttagagt caaagaatga attggagaat tttcctctaa acacaatctc gcattgtcaa
      721 gcagtggtac atgcatgcag ctatgactcc attctcgcct tggtatgcaa agagccaacg
      781 cagagcaagc cagaccttca ccttttccag tgtgatgagg ttaaggcaaa cctaattagt
      841 gaagatatcg aaagtgcaat cagtgacagt aaaggtggga aacagaagag gcggccggag
      901 gccctgagga tgattgccaa agcagatcct ggcatccctc ctcctcccag agctcctgcc
      961 cctgtgccac cagggactgt cacacaggtg gacgttagga gtcgcgtagc agcctggtct
     1021 gcctgggcag ctgaccaggg cgacttcgag aagccccggc agtaccacga gcaagaagag
     1081 acgcccgaga tgatggcagc ccggatcgac agggatgtgc aaatcttaaa ccatattttg
     1141 gatgacattg aattttttat caccaaactc caaaaagccg ccgaagcgtt ttctgagctt
     1201 tctaaaagga agaaaagtaa gaaaagtaaa aggaaaggac ctggagaggg cgttttaaca
     1261 ctgagggcaa aaccgccacc tcctgatgaa tttgttgact gtttccagaa gtttaaacat
     1321 ggattcaacc ttctggccaa gttgaagtcc catatccaga acccgagtgc ttcagatctg
     1381 gttcattttt tgtttactcc actaaatatg gtggtccagg caacaggtgg ccccgaactg
     1441 gccagttcgg tactcagccc actgttgaca aaagacacag ttgatttctt aaactacaca
     1501 gccactgcgg aggaacggaa gctgtggatg tcactgggag atagttgggt gaaagtgaga
     1561 gcagagtggc cgaaagaaca gttcatccca ccttacgtcc cgaggttccg caacggctgg
     1621 gagcccccga tgctgaactt catgggcgcg cccacagagc aagacatgta tcaactggcc
     1681 gagtccgtgg ccaacgcaga acaccagcgc aaacaggaca gcaagaggct gtccacagag
     1741 cattccaatg tgtccgacta tcctccagcc gacggatatg cgtacagtag cagcatgtac
     1801 cacagaggac cacatgcaga ccacggggag gctgccatgc ctttcaagtc aactcctaat
     1861 caccaagtag ataggaatta tgacgcagtc aaaacacaac ccaagaaata cgccaaatcc
     1921 aagtacgact ttgtggcgag gaacagcagc gagctctcgg ttatgaaaga tgatgtctta
     1981 gagatactcg acgatcgaag gcagtggtgg aaagtccgga atgccagtgg agactctggg
     2041 tttgtgccaa ataacattct ggatatcatg agaactccgg aatctggagt ggggcgcgct
     2101 gaccccccat acacacatac catacagaaa caaaggacgg aatacggcct gagatcagct
     2161 gacactcctt ctgccccatc accccctcca acgccagcac ccgttccggt cccccttcca
     2221 ccttctgtac cagcacccgt ttctgtgccc aaggtcccag ccaatgtcac ccgccagaac
     2281 agcagctcca gtgacagtgg gggcagcatt gtgcgggaca gccagagata caaacaactc
     2341 ccagtggacc gaaggaagtc ccagatggaa gaggttcagg atgagctctt ccagaggctg
     2401 accatcgggc gcagtgctgc acagaggaag ttccacgtgc cacggcagaa cgttccagtg
     2461 atcaatatca cttatgactc ctcaccggaa gaagtaaaga cttggctgca gtcaaaggga
     2521 ttcaaccccg tgactgtcaa tagcctcggg gtgttgaacg gagcacaact cttttctctc
     2581 aacaaagacg aactgaggtc tgtctgcccg gaaggtgcca gagtctttaa ccaaatcact
     2641 gttcagaaag ctgctttgga ggacagtaat ggaagctccg agttacaaga gatcatgcgg
     2701 agacggcagg agaagatcag cgccgctgcg agcgactcgg gagtggagtc tttcgatgaa
     2761 gggagcagcc actgagtcca tgaacttcct tattcttggt gtggtcattg aacagtgatg
     2821 gacatgcttt gttttaagaa gccttgaagg gaatgtcaaa gctgtcgtct tggtatatgt
     2881 aatttatcgc catataaaga aacagtatat gcctgagtaa gcagaggacc cgctgcttct
     2941 gtgcacatta gtttgattaa aactgagaag cgggtaggtg agatggctca gcaagtaaag
     3001 gtgcttgctg ccaagcccaa tgacccaagt tcgagtccct gggtctacat ggtaggagag
     3061 agctggcttc tgcaagttgt cctctgatca ccacacataa ataaataaca aatgtaattt
     3121 acaaactttt aaaagaaaat gtaatttaaa aagccagacg ttctagactg ttctgggctt
     3181 gggaaatatt ttttttcact ttcctaagat gtactttcct ttgctacatt aattatcgca
     3241 gccttgttcg atgatctaag tggggatatt tgacaatggc agatttattc attgcaacaa
     3301 ggaaagacac agccattgat gaaaaaaaag aaagtctcag ctttcagtga ctgggatacc
     3361 tgttgtccag ggaggaggct cagttagact accctctgct tacttgaggt ctgacatgcc
     3421 caatgagagt gtatttagct ttatttaaag ttcttaatgc caacagtttt aaaaatcaca
     3481 tttaaatgaa ctgtacaagg tagccagacc ttgaatgtat gaatagacta tataatatgt
     3541 ccccgagaaa ctttgttact ctcagctctg ttgattgcga aatcttgcat agattatgct
     3601 ttgatttagt ttcttttctg cttattgtgt aaaatgtaat taaacatgag aaagcacgag
     3661 tcaagagtaa ggcatatgca ctcacaatca atcaaagtag cccgtgtcgg tgtttattac
     3721 agtattttgc ttaatgaagg cctcagttat aaatattgat ataaataagt agtcaaagaa
     3781 tatcgggcca tcttatgtgt tttatctgtg taacagggac acttaattta catgtacttt
     3841 aaccccatga aagagtctag atagagagaa gacacatagc taaccttcaa caaatgttat
     3901 ttttggaaac acaggttttg tcatgaaacg taatgttatt tcacatatat aaaaaaaaga
     3961 tgttatgtaa gaatgttgta tattttaaca taagtccatt tagagagatt atctagattc
     4021 attaattttt catagtgcct ttttcacaaa agtcagctgg agagagtctg caataaacag
     4081 tatttgctgt atattaataa atagcttcta tctcatcaac accagaatgt ttgcttggta
     4141 gaatgagcat ttgggtcgaa cagaatgaga aaagtcccct gtgaaaggag gctccagtgt
     4201 gtgtctgtgc agagagtggc ctaggatact gccccgtgtt cccctgtttt ctgtctccct
     4261 tggtttctca tccctgcttc ctaccatcag ttcagagttg ataaaaacga gtgacaacca
     4321 acgcagactg catttgctat tgataactgc tagcaacttc tcagggccaa gtttccagac
     4381 gatgaagggc atggcagcct tgttttccat tcctgtctcc ccatcatcct tagcagatgt
     4441 gagatgagaa agaactagtt tggatgtctt atattctgta agcccttcat taaaagaaca
     4501 aagaggaaag ctctaaaagg caaaataaaa ttcaaatatt atgatca
//
