LOCUS       NM_001271587            4598 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus epidermal growth factor receptor pathway substrate 8
            (Eps8), transcript variant 4, mRNA.
ACCESSION   NM_001271587
VERSION     NM_001271587.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Dolat,L., Carpenter,V.K., Chen,Y.S., Suzuki,M., Smith,E.P.,
            Kuddar,O. and Valdivia,R.H.
  TITLE     Chlamydia repurposes the actin-binding protein EPS8 to disassemble
            epithelial tight junctions and promote infection
  JOURNAL   Cell Host Microbe 30 (12), 1685-1700 (2022)
   PUBMED   36395759
  REMARK    GeneRIF: Chlamydia repurposes the actin-binding protein EPS8 to
            disassemble epithelial tight junctions and promote infection.
REFERENCE   3  (bases 1 to 4598)
  AUTHORS   Akturk,A., Day,M. and Tarchini,B.
  TITLE     RGS12 polarizes the GPSM2-GNAI complex to organize and elongate
            stereocilia in sensory hair cells
  JOURNAL   Sci Adv 8 (42), eabq2826 (2022)
   PUBMED   36260679
REFERENCE   4  (bases 1 to 4598)
  AUTHORS   Halford,J., Bateschell,M. and Barr-Gillespie,P.G.
  TITLE     Ca2+ entry through mechanotransduction channels localizes BAIAP2L2
            to stereocilia tips
  JOURNAL   Mol Biol Cell 33 (4), br6 (2022)
   PUBMED   35044843
REFERENCE   5  (bases 1 to 4598)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4598)
  AUTHORS   Biesova,Z., Piccoli,C. and Wong,W.T.
  TITLE     Isolation and characterization of e3B1, an eps8 binding protein
            that regulates cell growth
  JOURNAL   Oncogene 14 (2), 233-241 (1997)
   PUBMED   9010225
REFERENCE   7  (bases 1 to 4598)
  AUTHORS   Avantaggiato,V., Torino,A., Wong,W.T., Di Fiore,P.P. and Simeone,A.
  TITLE     Expression of the receptor tyrosine kinase substrate genes eps8 and
            eps15 during mouse development
  JOURNAL   Oncogene 11 (6), 1191-1198 (1995)
   PUBMED   7566980
REFERENCE   8  (bases 1 to 4598)
  AUTHORS   Karlsson,T., Songyang,Z., Landgren,E., Lavergne,C., Di Fiore,P.P.,
            Anafi,M., Pawson,T., Cantley,L.C., Claesson-Welsh,L. and Welsh,M.
  TITLE     Molecular interactions of the Src homology 2 domain protein Shb
            with phosphotyrosine residues, tyrosine kinase receptors and Src
            homology 3 domain proteins
  JOURNAL   Oncogene 10 (8), 1475-1483 (1995)
   PUBMED   7537362
REFERENCE   9  (bases 1 to 4598)
  AUTHORS   Wong,W.T., Carlomagno,F., Druck,T., Barletta,C., Croce,C.M.,
            Huebner,K., Kraus,M.H. and Di Fiore,P.P.
  TITLE     Evolutionary conservation of the EPS8 gene and its mapping to human
            chromosome 12q23-q24
  JOURNAL   Oncogene 9 (10), 3057-3061 (1994)
   PUBMED   8084614
REFERENCE   10 (bases 1 to 4598)
  AUTHORS   Fazioli,F., Minichiello,L., Matoska,V., Castagnino,P., Miki,T.,
            Wong,W.T. and Di Fiore,P.P.
  TITLE     Eps8, a substrate for the epidermal growth factor receptor kinase,
            enhances EGF-dependent mitogenic signals
  JOURNAL   EMBO J 12 (10), 3799-3808 (1993)
   PUBMED   8404850
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166347.2 and AC093120.4.
            
            On Jul 21, 2022 this sequence version replaced NM_001271587.1.
            
            Transcript Variant: This variant (4) contains a distinct 5' UTR and
            initiates translation at an alternate start codon, compared to
            variant 1. The resulting isoform (2) has a distinct N-terminus and
            is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155022.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-344               AC166347.2         130901-131244
            345-419             AC093120.4         185830-185904       c
            420-496             AC093120.4         184126-184202       c
            497-564             AC093120.4         175033-175100       c
            565-726             AC093120.4         173876-174037       c
            727-876             AC093120.4         173596-173745       c
            877-959             AC093120.4         169338-169420       c
            960-1096            AC093120.4         168661-168797       c
            1097-1170           AC093120.4         165951-166024       c
            1171-1297           AC093120.4         163579-163705       c
            1298-1386           AC093120.4         161211-161299       c
            1387-1461           AC093120.4         160786-160860       c
            1462-1610           AC093120.4         158657-158805       c
            1611-1791           AC093120.4         155590-155770       c
            1792-1925           AC093120.4         151896-152029       c
            1926-2034           AC093120.4         147043-147151       c
            2035-2178           AC093120.4         146090-146233       c
            2179-2401           AC093120.4         136725-136947       c
            2402-2582           AC093120.4         130050-130230       c
            2583-2712           AC093120.4         128660-128789       c
            2713-4598           AC093120.4         123752-125637       c
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 66.78 cM"
     gene            1..4598
                     /gene="Eps8"
                     /note="epidermal growth factor receptor pathway substrate
                     8"
                     /db_xref="GeneID:13860"
                     /db_xref="MGI:MGI:104684"
     exon            1..344
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..2826
                     /gene="Eps8"
                     /note="isoform 2 is encoded by transcript variant 4;
                     epidermal growth factor receptor kinase substrate 8"
                     /codon_start=1
                     /product="epidermal growth factor receptor kinase
                     substrate 8 isoform 2"
                     /protein_id="NP_001258516.1"
                     /db_xref="GeneID:13860"
                     /db_xref="MGI:MGI:104684"
                     /translation="MLSQSCLDSCLDEFYLSSAGRGVRAGMNGHMSNRSSGYGVYPSQ
                     LNGYGSSPPYSQMDREHSSRTSAKALYEQRKNYARDSVSSVSDVSQYRVEHLTTFVLD
                     RKDAMITVEDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIS
                     HCQAVVHACSYDSILALVCKEPTQSKPDLHLFQCDEVKANLISEDIESAISDSKGGKQ
                     KRRPEALRMIAKADPGIPPPPRAPAPVPPGTVTQVDVRSRVAAWSAWAADQGDFEKPR
                     QYHEQEETPEMMAARIDRDVQILNHILDDIEFFITKLQKAAEAFSELSKRKKSKKSKR
                     KGPGEGVLTLRAKPPPPDEFVDCFQKFKHGFNLLAKLKSHIQNPSASDLVHFLFTPLN
                     MVVQATGGPELASSVLSPLLTKDTVDFLNYTATAEERKLWMSLGDSWVKVRAEWPKEQ
                     FIPPYVPRFRNGWEPPMLNFMGAPTEQDMYQLAESVANAEHQRKQDSKRLSTEHSNVS
                     DYPPADGYAYSSSMYHRGPHADHGEAAMPFKSTPNHQVDRNYDAVKTQPKKYAKSKYD
                     FVARNSSELSVMKDDVLEILDDRRQWWKVRNASGDSGFVPNNILDIMRTPESGVGRAD
                     PPYTHTIQKQRTEYGLRSADTPSAPSPPPTPAPVPVPLPPSVPAPVSVPKVPANVTRQ
                     NSSSSDSGGSIVRDSQRYKQLPVDRRKSQMEEVQDELFQRLTIGRSAAQRKFHVPRQN
                     VPVINITYDSSPEEVKTWLQSKGFNPVTVNSLGVLNGAQLFSLNKDELRSVCPEGARV
                     FNQITVQKAALEDSNGSSELQEIMRRRQEKISAAASDSGVESFDEGSSH"
     exon            345..419
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            420..496
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            497..564
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            565..726
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            727..876
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            877..959
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1096
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1170
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1297
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1386
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1461
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1610
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1791
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1925
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2034
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2178
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2401
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2402..2582
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2583..2712
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2713..4598
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4123..4128
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: AATAAA"
     regulatory      4147..4152
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: AATAAA"
     polyA_site      4148
                     /gene="Eps8"
                     /note="major polyA site"
     polyA_site      4170
                     /gene="Eps8"
                     /note="major polyA site"
     regulatory      4351..4356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: GATAAA"
     polyA_site      4372
                     /gene="Eps8"
     regulatory      4575..4580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps8"
                     /note="hexamer: AATAAA"
     polyA_site      4598
                     /gene="Eps8"
ORIGIN      
        1 atagtaacca cagctagcat taacctagca cccagaccga gttctccatc aatgcttccc
       61 attgttattt gctgctctgt tagtctcccc acctcgggga agggctttac ctcctcagcg
      121 gtggtgactt ccttgtttaa aagaggagga agtcttagct gaggcctggg agagtccaaa
      181 ggagagactt tgatgagtat agcggaacac cgaaactgaa gcagtcccac cctctcggtg
      241 tatgaaccaa aggtcactgg acacatccgt gagcagggag cgatgctctc tcagtcttgc
      301 cttgacagtt gcttggacga gttttacctc agcagtgcag gaagaggcgt gagagccggc
      361 atgaatggtc atatgtctaa ccgctccagt gggtatggag tctacccttc tcaactgaat
      421 ggttacggat cttcaccacc ctattcccag atggacagag aacacagctc aagaacaagt
      481 gcaaaggccc tttatgaaca aaggaagaac tatgcccgag acagtgtcag cagtgtgtcg
      541 gacgtgtccc agtaccgcgt ggaacacttg accaccttcg tgctggatcg gaaagatgca
      601 atgatcactg tcgaggacgg aataagaaag ctgaagttgc tggatgccaa gggcaaagtg
      661 tggactcaag atatgattct ccaagtggat gaccgagctg tgagcctgat tgacttagag
      721 tcaaagaatg aattggagaa ttttcctcta aacacaatct cgcattgtca agcagtggta
      781 catgcatgca gctatgactc cattctcgcc ttggtatgca aagagccaac gcagagcaag
      841 ccagaccttc accttttcca gtgtgatgag gttaaggcaa acctaattag tgaagatatc
      901 gaaagtgcaa tcagtgacag taaaggtggg aaacagaaga ggcggccgga ggccctgagg
      961 atgattgcca aagcagatcc tggcatccct cctcctccca gagctcctgc ccctgtgcca
     1021 ccagggactg tcacacaggt ggacgttagg agtcgcgtag cagcctggtc tgcctgggca
     1081 gctgaccagg gcgacttcga gaagccccgg cagtaccacg agcaagaaga gacgcccgag
     1141 atgatggcag cccggatcga cagggatgtg caaatcttaa accatatttt ggatgacatt
     1201 gaatttttta tcaccaaact ccaaaaagcc gccgaagcgt tttctgagct ttctaaaagg
     1261 aagaaaagta agaaaagtaa aaggaaagga cctggagagg gcgttttaac actgagggca
     1321 aaaccgccac ctcctgatga atttgttgac tgtttccaga agtttaaaca tggattcaac
     1381 cttctggcca agttgaagtc ccatatccag aacccgagtg cttcagatct ggttcatttt
     1441 ttgtttactc cactaaatat ggtggtccag gcaacaggtg gccccgaact ggccagttcg
     1501 gtactcagcc cactgttgac aaaagacaca gttgatttct taaactacac agccactgcg
     1561 gaggaacgga agctgtggat gtcactggga gatagttggg tgaaagtgag agcagagtgg
     1621 ccgaaagaac agttcatccc accttacgtc ccgaggttcc gcaacggctg ggagcccccg
     1681 atgctgaact tcatgggcgc gcccacagag caagacatgt atcaactggc cgagtccgtg
     1741 gccaacgcag aacaccagcg caaacaggac agcaagaggc tgtccacaga gcattccaat
     1801 gtgtccgact atcctccagc cgacggatat gcgtacagta gcagcatgta ccacagagga
     1861 ccacatgcag accacgggga ggctgccatg cctttcaagt caactcctaa tcaccaagta
     1921 gataggaatt atgacgcagt caaaacacaa cccaagaaat acgccaaatc caagtacgac
     1981 tttgtggcga ggaacagcag cgagctctcg gttatgaaag atgatgtctt agagatactc
     2041 gacgatcgaa ggcagtggtg gaaagtccgg aatgccagtg gagactctgg gtttgtgcca
     2101 aataacattc tggatatcat gagaactccg gaatctggag tggggcgcgc tgacccccca
     2161 tacacacata ccatacagaa acaaaggacg gaatacggcc tgagatcagc tgacactcct
     2221 tctgccccat caccccctcc aacgccagca cccgttccgg tcccccttcc accttctgta
     2281 ccagcacccg tttctgtgcc caaggtccca gccaatgtca cccgccagaa cagcagctcc
     2341 agtgacagtg ggggcagcat tgtgcgggac agccagagat acaaacaact cccagtggac
     2401 cgaaggaagt cccagatgga agaggttcag gatgagctct tccagaggct gaccatcggg
     2461 cgcagtgctg cacagaggaa gttccacgtg ccacggcaga acgttccagt gatcaatatc
     2521 acttatgact cctcaccgga agaagtaaag acttggctgc agtcaaaggg attcaacccc
     2581 gtgactgtca atagcctcgg ggtgttgaac ggagcacaac tcttttctct caacaaagac
     2641 gaactgaggt ctgtctgccc ggaaggtgcc agagtcttta accaaatcac tgttcagaaa
     2701 gctgctttgg aggacagtaa tggaagctcc gagttacaag agatcatgcg gagacggcag
     2761 gagaagatca gcgccgctgc gagcgactcg ggagtggagt ctttcgatga agggagcagc
     2821 cactgagtcc atgaacttcc ttattcttgg tgtggtcatt gaacagtgat ggacatgctt
     2881 tgttttaaga agccttgaag ggaatgtcaa agctgtcgtc ttggtatatg taatttatcg
     2941 ccatataaag aaacagtata tgcctgagta agcagaggac ccgctgcttc tgtgcacatt
     3001 agtttgatta aaactgagaa gcgggtaggt gagatggctc agcaagtaaa ggtgcttgct
     3061 gccaagccca atgacccaag ttcgagtccc tgggtctaca tggtaggaga gagctggctt
     3121 ctgcaagttg tcctctgatc accacacata aataaataac aaatgtaatt tacaaacttt
     3181 taaaagaaaa tgtaatttaa aaagccagac gttctagact gttctgggct tgggaaatat
     3241 tttttttcac tttcctaaga tgtactttcc tttgctacat taattatcgc agccttgttc
     3301 gatgatctaa gtggggatat ttgacaatgg cagatttatt cattgcaaca aggaaagaca
     3361 cagccattga tgaaaaaaaa gaaagtctca gctttcagtg actgggatac ctgttgtcca
     3421 gggaggaggc tcagttagac taccctctgc ttacttgagg tctgacatgc ccaatgagag
     3481 tgtatttagc tttatttaaa gttcttaatg ccaacagttt taaaaatcac atttaaatga
     3541 actgtacaag gtagccagac cttgaatgta tgaatagact atataatatg tccccgagaa
     3601 actttgttac tctcagctct gttgattgcg aaatcttgca tagattatgc tttgatttag
     3661 tttcttttct gcttattgtg taaaatgtaa ttaaacatga gaaagcacga gtcaagagta
     3721 aggcatatgc actcacaatc aatcaaagta gcccgtgtcg gtgtttatta cagtattttg
     3781 cttaatgaag gcctcagtta taaatattga tataaataag tagtcaaaga atatcgggcc
     3841 atcttatgtg ttttatctgt gtaacaggga cacttaattt acatgtactt taaccccatg
     3901 aaagagtcta gatagagaga agacacatag ctaaccttca acaaatgtta tttttggaaa
     3961 cacaggtttt gtcatgaaac gtaatgttat ttcacatata taaaaaaaag atgttatgta
     4021 agaatgttgt atattttaac ataagtccat ttagagagat tatctagatt cattaatttt
     4081 tcatagtgcc tttttcacaa aagtcagctg gagagagtct gcaataaaca gtatttgctg
     4141 tatattaata aatagcttct atctcatcaa caccagaatg tttgcttggt agaatgagca
     4201 tttgggtcga acagaatgag aaaagtcccc tgtgaaagga ggctccagtg tgtgtctgtg
     4261 cagagagtgg cctaggatac tgccccgtgt tcccctgttt tctgtctccc ttggtttctc
     4321 atccctgctt cctaccatca gttcagagtt gataaaaacg agtgacaacc aacgcagact
     4381 gcatttgcta ttgataactg ctagcaactt ctcagggcca agtttccaga cgatgaaggg
     4441 catggcagcc ttgttttcca ttcctgtctc cccatcatcc ttagcagatg tgagatgaga
     4501 aagaactagt ttggatgtct tatattctgt aagcccttca ttaaaagaac aaagaggaaa
     4561 gctctaaaag gcaaaataaa attcaaatat tatgatca
//
