LOCUS       NM_007943               5193 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus epidermal growth factor receptor pathway substrate 15
            (Eps15), transcript variant 1, mRNA.
ACCESSION   NM_007943
VERSION     NM_007943.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Wang,B., Tang,X., Yao,L., Wang,Y., Chen,Z., Li,M., Wu,N., Wu,D.,
            Dai,X., Jiang,H. and Ai,D.
  TITLE     Disruption of USP9X in macrophages promotes foam cell formation and
            atherosclerosis
  JOURNAL   J Clin Invest 132 (10) (2022)
   PUBMED   35389885
REFERENCE   3  (bases 1 to 5193)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5193)
  AUTHORS   Day,K.J., Kago,G., Wang,L., Richter,J.B., Hayden,C.C., Lafer,E.M.
            and Stachowiak,J.C.
  TITLE     Liquid-like protein interactions catalyse assembly of endocytic
            vesicles
  JOURNAL   Nat Cell Biol 23 (4), 366-376 (2021)
   PUBMED   33820972
  REMARK    GeneRIF: Liquid-like protein interactions catalyse assembly of
            endocytic vesicles.
REFERENCE   5  (bases 1 to 5193)
  AUTHORS   Pereira,M., Ko,J.H., Logan,J., Protheroe,H., Kim,K.B., Tan,A.L.M.,
            Croucher,P.I., Park,K.S., Rotival,M., Petretto,E., Bassett,J.D.,
            Williams,G.R. and Behmoaras,J.
  TITLE     A trans-eQTL network regulates osteoclast multinucleation and bone
            mass
  JOURNAL   Elife 9, e55549 (2020)
   PUBMED   32553114
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5193)
  AUTHORS   Coda,L., Salcini,A.E., Confalonieri,S., Pelicci,G., Sorkina,T.,
            Sorkin,A., Pelicci,P.G. and Di Fiore,P.P.
  TITLE     Eps15R is a tyrosine kinase substrate with characteristics of a
            docking protein possibly involved in coated pits-mediated
            internalization
  JOURNAL   J Biol Chem 273 (5), 3003-3012 (1998)
   PUBMED   9446614
REFERENCE   7  (bases 1 to 5193)
  AUTHORS   Tebar,F., Sorkina,T., Sorkin,A., Ericsson,M. and Kirchhausen,T.
  TITLE     Eps15 is a component of clathrin-coated pits and vesicles and is
            located at the rim of coated pits
  JOURNAL   J Biol Chem 271 (46), 28727-28730 (1996)
   PUBMED   8910509
REFERENCE   8  (bases 1 to 5193)
  AUTHORS   Avantaggiato,V., Torino,A., Wong,W.T., Di Fiore,P.P. and Simeone,A.
  TITLE     Expression of the receptor tyrosine kinase substrate genes eps8 and
            eps15 during mouse development
  JOURNAL   Oncogene 11 (6), 1191-1198 (1995)
   PUBMED   7566980
REFERENCE   9  (bases 1 to 5193)
  AUTHORS   Schumacher,C., Knudsen,B.S., Ohuchi,T., Di Fiore,P.P.,
            Glassman,R.H. and Hanafusa,H.
  TITLE     The SH3 domain of Crk binds specifically to a conserved
            proline-rich motif in Eps15 and Eps15R
  JOURNAL   J Biol Chem 270 (25), 15341-15347 (1995)
   PUBMED   7797522
REFERENCE   10 (bases 1 to 5193)
  AUTHORS   Fazioli,F., Minichiello,L., Matoskova,B., Wong,W.T. and Di
            Fiore,P.P.
  TITLE     eps15, a novel tyrosine kinase substrate, exhibits transforming
            activity
  JOURNAL   Mol Cell Biol 13 (9), 5814-5828 (1993)
   PUBMED   7689153
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB858255.1, BC048783.1, AK083176.1 and AW124983.1.
            
            On May 7, 2009 this sequence version replaced NM_007943.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (A). Isoform A mainly
            localizes to clathrin-coated pits at the plasma membrane.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.91429.1,
                                           SRR1660815.219512.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BB858255.1         2-27
            27-2981             BC048783.1         1-2955
            2982-3932           AK083176.1         2017-2967
            3933-5021           BC048783.1         3905-4993
            5022-5193           AW124983.1         1-172               c
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 51.29 cM"
     gene            1..5193
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /note="epidermal growth factor receptor pathway substrate
                     15"
                     /db_xref="GeneID:13858"
                     /db_xref="MGI:MGI:104583"
     exon            1..170
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..2831
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /note="isoform A is encoded by transcript variant 1;
                     epidermal growth factor receptor substrate 15; protein
                     AF-1p; epidermal growth factor pathway substrate 15"
                     /codon_start=1
                     /product="epidermal growth factor receptor substrate 15
                     isoform A"
                     /protein_id="NP_031969.1"
                     /db_xref="CCDS:CCDS18462.1"
                     /db_xref="GeneID:13858"
                     /db_xref="MGI:MGI:104583"
                     /translation="MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLK
                     KSGLPDLILGKIWDLADTDGKGVLSKQEFFVALRLVACAQNGLEVSLSSLSLAVPPPR
                     FHDSSSPLLTSGPSVAELPWAVKSEDKAKYDAIFDSLSPVDGFLSGDKVKPVLLNSKL
                     PVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTW
                     VVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSALLAHIWSLCDTKGCGK
                     LSKDQFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNITGSSPVADFSAIKE
                     LDTLNNEIVDLQREKNNVEQDLKEKEDTVKQRTSEVQDLQDEVQRESINLQKLQAQKQ
                     QVQELLGELDEQKAQLEEQLQEVRKKCAEEAQLISSLKAEITSQESQISSYEEELLKA
                     REELSRLQQETAQLEESVESGKAQLEPLQQHLQESQQEISSMQMRLEMKDLETDNNQS
                     NWSSSPQSVLVNGATDYCSLSTSSSETANFNEHAEGQNNLESEPTHQESSVRSSPEIA
                     PSDVTDESEAVTVAGNEKVTPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDP
                     FVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFTTSSTDPFSASSNSS
                     NTSVETWKHNDPFAPGGTVVAAASDSATDPFASVFGNESFGDGFADFSTLSKVNNEDA
                     FNPTISSSTSSVTIAKPMLEETASKSEDVPPALPPKVGTPTRPCPPPPGKRPINKLDS
                     SDPLKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSTTTNKEADPSNFANFSAYPSEEDM
                     IEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA"
     exon            171..212
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            213..302
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            303..350
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            351..446
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            447..512
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            513..638
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            639..698
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            699..788
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            789..934
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1091
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1177
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1250
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1412
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1610
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1811
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1928
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2019
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2055
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2192
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2262
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2263..2339
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2340..2502
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2684
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2685..5183
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5000..5005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5021
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
     regulatory      5152..5157
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5183
                     /gene="Eps15"
                     /gene_synonym="2410112D09Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtgaggaggg aagttgtcga agttcgggga gacgcccggc cgccggcctc gcctacggcc
       61 tgtccctccg cctccttccc gccccgggcc cccgtccgtc cgtccttcct tcccctcccg
      121 tgcatgatgg aaacaccatg gctgcggcag cccagctctc cctgacacag ttgtcaagtg
      181 ggaatcctgt atatgaaaaa tactacagac aggttgaggc aggcaatact ggaagggtgt
      241 tggcgttaga tgctgctgca ttcctgaaaa agtcagggct tccagacttg attcttggaa
      301 agatttggga tttagctgac acagatggca aaggtgtcct gagcaaacaa gaattctttg
      361 ttgctttacg gcttgtggca tgtgctcaga atggactgga agtttcactg agtagcctaa
      421 gtctggctgt tcctccacca agatttcatg actccagcag tccgttgcta accagtgggc
      481 cctcagttgc tgagctcccg tgggctgtaa agtctgaaga taaagccaaa tatgatgcaa
      541 tttttgacag tttaagccca gtggatggat ttttgtctgg tgataaagtg aaaccagtgt
      601 tgctcaactc taagttacct gtggaaatcc ttggaagagt ttgggagttg agtgatattg
      661 accacgatgg aaagctggac agagatgagt ttgcagttgc catgtttttg gtatactgtg
      721 cactggagaa agaacctgtg ccaatgtcct tgcctccagc cttggtgcca ccttctaaga
      781 gaaaaacgtg ggttgtatcc cctgcagaaa aagctaaata tgatgaaatt tttctgaaaa
      841 ctgataagga tatggatgga tatgtgtctg gactggaggt ccgtgaaacc ttcctgaaaa
      901 caggtttacc ttctgccttg ctagcccaca tttggtcact atgtgacaca aagggctgtg
      961 ggaagctttc aaaagaccag tttgccttgg cttttcactt aatcaatcag aagttaataa
     1021 aaggcattga ccctcctcat agtctcactc ctgagatgat tccaccatca gacagatcca
     1081 gtttacaaaa gaacatcaca ggatcaagtc ctgttgcaga tttttctgct attaaggaac
     1141 tagataccct taacaatgaa atagttgacc tgcagaggga aaagaacaat gtggagcagg
     1201 accttaaaga gaaggaagac acagttaagc agaggaccag tgaggttcag gatcttcaag
     1261 atgaagttca aagggagagt attaatctac aaaaactgca ggcccagaag cagcaggtgc
     1321 aggagctcct gggtgaactg gatgagcaga aagcccagct ggaggagcag ctccaggaag
     1381 tcaggaaaaa gtgtgctgag gaggcccagc tgatttcttc cctgaaagca gaaataacta
     1441 gtcaagaatc tcagatctcc agttatgagg aagaactgtt gaaagctaga gaagaactaa
     1501 gtcgcctaca acaagaaaca gcacaattgg aagaaagtgt ggagtcaggg aaggctcagc
     1561 tggaacctct tcagcagcac ctacaagagt cacaacagga aatcagctca atgcaaatga
     1621 gattggaaat gaaagatctg gaaactgata ataaccaatc aaattggagc agtagcccac
     1681 aaagcgttct tgttaatggt gctacagatt actgtagcct cagcaccagc agcagtgaaa
     1741 cagccaactt caacgaacat gctgaaggcc aaaacaacct agagtctgaa cccacacacc
     1801 aggagtcctc agtaagaagt agtcctgaaa tcgcaccttc tgatgtgact gatgaaagtg
     1861 aggctgtgac tgtggctggt aatgagaaag ttactccgag atttgacgat gacaagcact
     1921 caaaagagga agatccattt aatgtagaat caagttcact gacagatgca gttgcagata
     1981 caaacttgga ttttttccag tctgatcctt ttgttggcag tgatcctttc aaggatgatc
     2041 cttttggaaa aattgatcca tttggtggtg accctttcaa aggctcagat ccttttgcgt
     2101 ctgattgctt ctttaagcag acttctactg atccttttac cacttcaagt acggaccctt
     2161 tcagtgcatc cagcaacagc agtaatacat cggtagaaac ttggaagcac aatgacccat
     2221 ttgctcctgg tggaacagtt gttgctgcag cgagtgattc agccacagac ccttttgctt
     2281 ctgttttcgg aaatgaatca tttggagatg gatttgctga cttcagcaca ttatcaaagg
     2341 tcaacaatga agatgctttt aatcctacca tatcaagttc taccagcagt gtgaccattg
     2401 caaaacctat gttagaggaa acagccagca agagtgaaga tgtgcctcca gcactgccgc
     2461 ccaaagttgg cactccaaca agaccttgcc cgccaccccc tgggaaaaga cccatcaaca
     2521 aattggattc ttctgatccc cttaaactga atgatccatt tcagcctttc ccaggcaatg
     2581 atagtcccaa agaaaaagat cctgatatgt tttgtgatcc attcacttct tctaccacta
     2641 ccaataaaga ggctgaccca agcaattttg ctaacttcag tgcttatccc tctgaagaag
     2701 atatgattga atgggcaaaa agggaaagtg agcgggaaga agaacagagg cttgccagac
     2761 taaatcagca ggagcaagaa gacttggaac tggccattgc acttagcaaa tctgagatct
     2821 cagaagcatg aagagttatc tgtcctttgt cagcagtaca gtgctctctg gaacactgaa
     2881 gctatttacc atgtgcatca aactacctat gagcatggga tacaaaaggt ttgagattcc
     2941 tagaaatgtg acaaaagtct agtttgtttt tttttttttt tttggggggg ggtgctattt
     3001 caaatgtgtc ttttattttt tcttccaaaa gcagtaccct aattaaacgg ctttgcctag
     3061 acccctgttc tggtgtgcat ttatagtgag ctcttgcctg cttgcactag cccctgtaaa
     3121 gctagagcat caagcctctg ccttctggaa cctagcagat gtagttttgc gctgtgctac
     3181 ccttgtcctg tagttactat gacaacgact ggagttctga aagtgtgcaa gtattctccc
     3241 tgagctgagt gcttgaagga gggggaggga aagagtatcc tgtttcttct gatcggtaca
     3301 aattagtcag tttaaagtgc attatatgta cttctggaga aaatatagtt aagttactta
     3361 gagacagggg aaaatattct gcatagatct actccactat tgaaaactgg ataagttaaa
     3421 ccattattgc tccagccgca agtaattcct aaataaaagt attaaagcct tgtatatgaa
     3481 acatgttttt agaacaattt tctctggact taaagaattt tacgattaac agatacctca
     3541 cgacatgttt cctagttaaa aacaaacaaa ccaatttctt acaaataaaa tttgtaagga
     3601 tatgttcatt ttaagtgcta gtgaggaaaa tttgtaactt aaaatataac ctatccgttt
     3661 aagcaaacac ttagcctgta cccattttgc ctttgtggct gcactgaccg tagttcataa
     3721 gtgacctgaa gtctaggaag ctagaattgg aaggtacttt ggtattcatt atgtgttttg
     3781 tttctttagt gaaggcccag ctatttgcag tgatacaaat ggaagaaata acatttgtta
     3841 tatcctctta ctttctgtcc atctttgtag tttaaatttc tttatttgct ttatttgtct
     3901 caccaataaa attgacaagc taccccaccc ccaaaaaaat cctagttttt aaattgagag
     3961 aaaaataaac caagttgcca aattatcaga gccctacttt tgttaaaagc ccatcttctg
     4021 ctgaactggc ctgacagaca gctggatttt aaatggattt gaagtaaact ataatttgta
     4081 attttttaga taagagttac taatttagtg tttgtaatac caagataaac aaaaatgcac
     4141 ctacaaacta tttaaaattc tttaggatag ccaagttaag gtggtattga actgttgtta
     4201 gagtaattta ctgcctggcc tataagtaaa ttctagtgtc taatcagacc attttgaaat
     4261 ggtgcttttt tttcttttcc acatactatt ggatagctgg gatttaaaac aaacttcaac
     4321 cactttcttg cactgctaac ttttttctac ttttgtaaat aaatatttca gcataaaagt
     4381 taaaacatga agtaagggct gaatcgtaat cacaaggctt taattgtgat aactaattcc
     4441 gtggcctaag tgcttgctgc aaagggtgat gagcaggtca ggttgggtgg tgttcctgtt
     4501 tgccccttcc tctcactcct caggagaaag gtaagttgac ttgaataacc ttcgtttgca
     4561 ctgagaaaag tgaaaaatgt ttgaaatgct ttaatttttt ttaataaaaa acactctgga
     4621 aatattaaat atctataact tataaacagc aacttttgat gtgtagtaag tgtgccctaa
     4681 tcttctgtgc gcttagctgg gtcatagagg ttcttatcct ttgttaagat gtgtgtgctc
     4741 agtgggccaa tatcatacta aagtatgtaa tatgacataa acatatctat atctccatgc
     4801 ttgagtgtat agaatgaatg tcttaagagc catttgtgat gttttatggt gttgaccctg
     4861 gctccaaagc ctgcgcttga aacaaagaca gagttttaac catcttcctt ttatgtactt
     4921 gttcagcttt atctgtgctc tcttcataga tgttctctca tgtttcaaac ttataaatta
     4981 ctgttgtaat tatgaagata ataaacttta ataagtaaaa atgtgtacta tatcattttg
     5041 cattgaaact ttgtgctgtt cttgatcgtg tgcatatggt aggaggaggg caagttgcca
     5101 tcagcataca ttatcctgta taaagggttc ttacagtact atagagactt aaataaatgt
     5161 tgaatgctaa ttctataaac aaaaaaaaaa aaa
//
