LOCUS       NM_001357474            4927 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus glutamyl-prolyl-tRNA synthetase 1 (Eprs1), transcript
            variant 2, mRNA.
ACCESSION   NM_001357474 XM_006497117
VERSION     NM_001357474.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Wu,J., Hollinger,J., Bonanno,E., Jiang,F. and Yao,P.
  TITLE     Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA Synthetase
            Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy
  JOURNAL   Cells 13 (1), 35 (2023)
   PUBMED   38201239
  REMARK    GeneRIF: Cardiomyocyte-Specific Loss of Glutamyl-prolyl-tRNA
            Synthetase Leads to Disturbed Protein Homeostasis and Dilated
            Cardiomyopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4927)
  AUTHORS   Lee,E.Y., Kim,S.M., Hwang,J.H., Jang,S.Y., Park,S., Choi,S.,
            Lee,G.S., Hwang,J., Moon,J.H., Fox,P.L., Kim,S., Lee,C.H. and
            Kim,M.H.
  TITLE     Glutamyl-prolyl-tRNA synthetase 1 coordinates early endosomal
            anti-inflammatory AKT signaling
  JOURNAL   Nat Commun 13 (1), 6455 (2022)
   PUBMED   36309524
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4927)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4927)
  AUTHORS   Vasu,K., Ramachandiran,I., Terenzi,F., Khan,D., China,A., Khan,K.,
            Chechi,A., Baleanu-Gogonea,C., Gogonea,V. and Fox,P.L.
  TITLE     The zinc-binding domain of mammalian prolyl-tRNA synthetase is
            indispensable for catalytic activity and organism viability
  JOURNAL   iScience 24 (3), 102215 (2021)
   PUBMED   33748704
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4927)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 4927)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   8  (bases 1 to 4927)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 4927)
  AUTHORS   Kaiser,E., Hu,B., Becher,S., Eberhard,D., Schray,B., Baack,M.,
            Hameister,H. and Knippers,R.
  TITLE     The human EPRS locus (formerly the QARS locus): a gene encoding a
            class I and a class II aminoacyl-tRNA synthetase
  JOURNAL   Genomics 19 (2), 280-290 (1994)
   PUBMED   8188258
REFERENCE   10 (bases 1 to 4927)
  AUTHORS   Fett,R. and Knippers,R.
  TITLE     The primary structure of human glutaminyl-tRNA synthetase. A highly
            conserved core, amino acid repeat regions, and homologies with
            translation elongation factors
  JOURNAL   J Biol Chem 266 (3), 1448-1455 (1991)
   PUBMED   1988429
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129195.3 and AC131980.9.
            
            On Nov 1, 2017 this sequence version replaced XM_006497117.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.158750.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-200               AC129195.3         15608-15807         c
            201-285             AC129195.3         11236-11320         c
            286-385             AC129195.3         9175-9274           c
            386-542             AC129195.3         7806-7962           c
            543-682             AC129195.3         5794-5933           c
            683-777             AC129195.3         4639-4733           c
            778-853             AC129195.3         1-76                c
            854-904             AC131980.9         196657-196707       c
            905-1097            AC131980.9         195821-196013       c
            1098-1269           AC131980.9         193587-193758       c
            1270-1503           AC131980.9         190448-190681       c
            1504-1588           AC131980.9         189228-189312       c
            1589-1648           AC131980.9         188440-188499       c
            1649-1669           AC131980.9         188181-188201       c
            1670-1780           AC131980.9         181085-181195       c
            1781-1917           AC131980.9         179392-179528       c
            1918-2125           AC131980.9         178291-178498       c
            2126-2238           AC131980.9         177052-177164       c
            2239-2356           AC131980.9         176219-176336       c
            2357-2716           AC131980.9         173890-174249       c
            2717-2950           AC131980.9         168364-168597       c
            2951-3213           AC131980.9         167805-168067       c
            3214-3265           AC131980.9         165322-165373       c
            3266-3475           AC131980.9         164191-164400       c
            3476-3548           AC131980.9         163783-163855       c
            3549-3628           AC131980.9         162324-162403       c
            3629-3730           AC131980.9         162008-162109       c
            3731-3886           AC131980.9         161073-161228       c
            3887-4084           AC131980.9         159607-159804       c
            4085-4258           AC131980.9         157173-157346       c
            4259-4419           AC131980.9         155271-155431       c
            4420-4498           AC131980.9         151169-151247       c
            4499-4563           AC131980.9         147642-147706       c
            4564-4927           AC131980.9         147198-147561       c
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 89.5 cM"
     gene            1..4927
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /note="glutamyl-prolyl-tRNA synthetase 1"
                     /db_xref="GeneID:107508"
                     /db_xref="MGI:MGI:97838"
     exon            1..200
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..4714
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /EC_number="6.1.1.15"
                     /EC_number="6.1.1.17"
                     /note="isoform 2 is encoded by transcript variant 2;
                     bifunctional aminoacyl-tRNA synthetase;
                     glutamine-proline-tRNA synthetase; bifunctional
                     glutamate/proline--tRNA ligase"
                     /codon_start=1
                     /product="bifunctional glutamate/proline--tRNA ligase
                     isoform 2"
                     /protein_id="NP_001344403.1"
                     /db_xref="GeneID:107508"
                     /db_xref="MGI:MGI:97838"
                     /translation="MAALCLTVNAGNPPLEALLAVEHVKGDVSISVEEGKENLLRVSE
                     TVAFTDVNSILRYLARIATTSGLYGTNLMEHTEIDHWLEFSATKLSSCDRLTSAINEL
                     NHCLSLRTYLVGNSLTLADLCVWATLKGSAAWQEHLKQNKTLVHVKRWFGFLEAQQAF
                     RSVGTKWDVSGNRATVAPDKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAA
                     LLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETI
                     MKYAEKLIQEGKAYVDDTPAEQMKAEREQRTESKHRKNSVEKNLQMWEEMKKGSQFGQ
                     SCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTH
                     ALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGW
                     DDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKLRALCKKVID
                     PVAPRYVALLKKEVVPVNVLDAQEEMKEVARHPKNPDVGLKPVWYSPKVFIEGADAET
                     FSEGEMVTFINWGNINITKIHKNADGKITSLDAKLNLENKDYKKTTKITWLAESTHAL
                     SIPAVCVTYEHLITKPVLGKDEDFKQYINKDSKHEELMLGDPCLKDLKKGDIIQLQRR
                     GFFICDQPYEPVSPYSCREAPCILIYIPDGHTKEMPTSGSKEKTKVEISKKETSSAPK
                     ERPAPAVSSTCATAEDSSVLYSRVAVQGDVVRELKAKKAPKEDIDAAVKQLLTLKAEY
                     KEKTGQEYKPGNPSAAAVQTVSTKSSSNTVESTSLYNKVAAQGEVVRKLKAEKAPKAK
                     VTEAVECLLSLKAEYKEKTGKDYVPGQPPASQNSHSNPVSNAQPAGAEKPEAKVLFDR
                     VACQGEVVRKLKAEKASKDQVDSAVQELLQLKAQYKSLTGIEYKPVSATGAEDKDKKK
                     KEKENKSEKQNKPQKQNDGQGKDSSKSQGSGLSSGGAGEGQGPKKQTRLGLEAKKEEN
                     LAEWYSQVITKSEMIEYYDVSGCYILRPWSYSIWESIKDFFDAEIKKLGVENCYFPIF
                     VSQAALEKEKNHIEDFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRD
                     LPVRLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAADEVLQILELYARVY
                     EELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASGRAIQGATSHHLGQNFSKMCEIV
                     FEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGLVLPPRVASVQVVVIPCGITN
                     ALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYSPGWKFNHWELKGVPVRLEVGP
                     RDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQLNLFTRASEDLKTHMVVSNT
                     LEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPGAPSMGAKSLCIPFNPLC
                     ELQPGAMCVCGKNPAKFYTLFGRSY"
     exon            201..285
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            286..385
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            386..542
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            543..682
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            683..777
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            778..904
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1097
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1269
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1503
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1588
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1648
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1669
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1780
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..1917
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2125
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2238
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2356
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2716
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2717..2950
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3213
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3214..3265
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3266..3475
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3476..3548
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3549..3628
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3629..3730
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3886
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3887..4084
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4085..4258
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4259..4419
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4420..4498
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4499..4563
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4564..4927
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4899..4904
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /note="hexamer: AATAAA"
     polyA_site      4922
                     /gene="Eprs1"
                     /gene_synonym="2410081F06Rik; 3010002K18Rik; Eprs; Qprs"
                     /note="major polyA site"
ORIGIN      
        1 gtagcgtgcg caccgactcc ggcggtggcg gcccgtgggg aggagtcagg gctgcggtgc
       61 cttcggtcgc gcgcacacgt tgcatctcct tgcttccacc tgaaccctcg ccgccaggaa
      121 ggcagcccga cgagtagacc gcagctcctg ggagatggcg gcgctgtgcc tcacggtgaa
      181 cgccggaaac cctccgctgg aagctctgct ggcggtggaa catgtgaaag gtgatgtcag
      241 catttctgtg gaagaaggga aagagaatct tcttcgggtt tctgagactg tggcattcac
      301 tgacgtgaat tcaatcctgc gctacctggc tagaattgca actacgtctg ggctgtatgg
      361 gactaacctg atggagcaca ctgagattga tcattggttg gagtttagtg ctacaaagtt
      421 gtcttcgtgt gataggctga cttctgcaat caacgagctt aatcattgcc tgtctctgag
      481 aacataccta gttgggaact ccttgacctt agcagatcta tgtgtttggg ccaccctaaa
      541 aggaagtgct gcatggcagg aacacttgaa acagaacaaa actctagtcc acgttaaacg
      601 ctggtttggc tttcttgagg cccagcaggc cttccgctca gttggcacca agtgggatgt
      661 ttcaggaaac agagctacag tggcacctga caaaaaacaa gatgttggga aattcgtgga
      721 gcttccaggt gcagagatgg gaaaggtgac agtcagattc cccccagagg ctagtggtta
      781 cttacacatt gggcatgcga aagctgctct tctgaaccag cactaccagg ttaactttaa
      841 agggaaactg atcatgagat ttgatgacac aaacccagaa aaggaaaagg aagattttga
      901 gaaggtcatc ttggaagatg ttgcaatgct gcatattaaa ccagatcagt ttacttacac
      961 ttcagatcat tttgaaacta taatgaagta tgcagagaaa ttaattcaag aagggaaggc
     1021 ttatgtggat gacactcctg cagaacagat gaaggcggaa cgtgaacaga ggacagaatc
     1081 caaacacagg aagaactctg ttgagaagaa tctacagatg tgggaagaaa tgaaaaaagg
     1141 aagccagttt ggtcagtcct gttgcctgcg agcaaaaatt gacatgagta gtaacaatgg
     1201 gtgtatgaga gacccgaccc tttaccgatg caaaatccag ccccacccaa ggactgggaa
     1261 taaatacaat gtttatccaa catatgactt tgcctgccct atagtggaca gcattgaagg
     1321 tgttactcat gccttgagga caacagagta ccacgaccga gatgaacaat tttattggat
     1381 cattgaagct ttaggcataa ggaaaccata catctgggag tacagtcgct taaatctcaa
     1441 caacaccgtg ctgtccaaaa gaaagctcac gtggtttgtc aatgaaggac tggtagatgg
     1501 atgggatgac ccacggtttc ctacagttcg tggagttctg agaagaggga tgacagttga
     1561 ggggctgaag cagttcattg ctgctcaggg ctcctcaagg tctgttgtga acatggaatg
     1621 ggacaaaatc tgggcgttca acaaaaagct gcgagctctc tgtaagaagg ttattgaccc
     1681 agtggctcca cggtacgttg cattgctgaa gaaggaagtc gtcccagtga atgtcctgga
     1741 tgcgcaggag gagatgaagg aagtagccag acaccccaag aatcctgatg ttggtttgaa
     1801 gcctgtgtgg tacagtccca aggttttcat agaaggggcc gatgcagaga ctttctcaga
     1861 gggtgagatg gtcacattta taaactgggg caacatcaac attactaaaa tacacaaaaa
     1921 tgcagatgga aaaattacat ctctagatgc aaaattgaat ttggaaaaca aagactacaa
     1981 gaaaaccacc aagatcactt ggcttgctga gagcacacac gctctctcga tcccagccgt
     2041 ctgtgtcact tatgagcact tgatcacaaa gccagtgctg ggaaaagatg aggacttcaa
     2101 gcagtacatc aacaaagaca gtaagcatga agagctaatg ctaggagatc cttgccttaa
     2161 agatctgaaa aaaggagaca tcatacaact gcagaggaga gggttcttca tatgcgacca
     2221 gccatacgaa cctgtcagcc catatagttg cagagaagct ccatgcatcc tgatatacat
     2281 acctgatggg cacacaaagg aaatgccaac atccggatca aaggaaaaga ccaaagtgga
     2341 aatctcaaaa aaggagacaa gctctgctcc taaagaaaga ccagcacctg ctgtgagttc
     2401 tacttgtgct acagctgagg attcctccgt cctctacagc agagtggcgg tgcaggggga
     2461 tgtggttcgt gaattgaaag ccaagaaagc gccaaaggaa gatatagacg cagctgtgaa
     2521 gcagcttttg actttgaaag ctgaatataa agagaaaact gggcaggagt ataaacctgg
     2581 gaacccctct gctgcagcag tacagactgt ttctacaaag tcatcatcaa acactgtgga
     2641 aagtacatct ttatacaata aagtcgctgc ccaaggggag gtggtccgaa agctgaaagc
     2701 ggaaaaggct cctaaggcta aagtaactga agctgtggaa tgtttgctgt ccctgaaagc
     2761 tgagtataaa gaaaagactg ggaaggacta tgtacctggc cagcccccag catcgcagaa
     2821 ctcacattca aaccctgtca gtaatgctca gcctgctgga gcggagaaac cagaagccaa
     2881 agtgctattt gacagagtgg cttgtcaagg agaagtagtt cgaaaactta aagctgaaaa
     2941 ggcctctaag gatcaagtgg attcggcggt acaagaactt cttcagctga aggcacagta
     3001 caagtcgctg acaggaatcg agtataagcc tgtgtctgct actggggcag aagacaaaga
     3061 taaaaagaag aaagaaaaag aaaataaatc tgaaaagcag aacaagcctc agaaacaaaa
     3121 tgatggccaa gggaaagact cttccaaaag ccaaggcagt ggcctgtcat caggtggagc
     3181 aggagaaggg caggggccta agaaacagac cagattgggt cttgaagcaa aaaaagaaga
     3241 aaatcttgcc gaatggtact ctcaagtcat cacaaagtcc gagatgattg aatactacga
     3301 tgtgagtggc tgctatatcc tccgtccctg gtcgtactcc atctgggagt ccattaagga
     3361 cttctttgac gctgagatca agaagctcgg tgttgagaac tgctacttcc ccatatttgt
     3421 gtctcaggct gcgttagaga aagagaagaa tcacatcgaa gactttgctc ctgaggttgc
     3481 ttgggttaca agatcaggaa aaacagagtt ggcagagcca attgccattc gtcctactag
     3541 tgaaacagtg atgtatccgg catatgctaa atgggtgcag tcccacagag acctgcccgt
     3601 caggctcaac cagtggtgca atgtggtgcg ctgggagttc aaacacccac agcctttcct
     3661 ccgaactcgg gagttcctgt ggcaggaggg gcacagcgcc tttgccacct ttgaggaagc
     3721 agctgatgag gttttacaga tccttgagtt atatgctcga gtatatgaag agctcttagc
     3781 cattcctgtt gtgagaggaa ggaagactga aaaggagaag tttgcaggag gagactacac
     3841 aaccacaata gaagccttca tatctgccag cggacgcgcc attcagggag caacatcaca
     3901 ccatttaggc cagaatttct ccaaaatgtg tgaaatagtt tttgaagatc ccaagacacc
     3961 aggagaaaag cagtttgcat accagtgctc ctggggcctg acaacacgaa ccatcggtgt
     4021 gatggtcatg gtccatgggg acaacatggg cctagtgctt ccaccccgag tagcatctgt
     4081 ccaggtggta gtcattccct gtggaatcac aaatgccctg tctgaagaag acagagaggc
     4141 actgatggca aaatgcaacg agtacagaag gcggctgctt ggtgctaaca tccgagtccg
     4201 agtcgatcta cgagacaact actccccagg ctggaagttc aaccactggg aactcaaggg
     4261 agttccagtc agacttgagg tggggccacg tgatatgaag agctgtcagt ttgtagcagt
     4321 cagacgagat actggagaga aactgacaat tgctgagaag gaggctgagg ctaagctgga
     4381 aaaggttttg gaagacatcc agctgaacct tttcacaagg gcttctgaag accttaaaac
     4441 ccacatggtt gtgtctaata cactggagga cttccagaag gtgctagatg ctggaaaggt
     4501 tgcacagatt ccattttgtg gggaaattga ctgtgaggac tggatcaaaa agatgactgc
     4561 cagggatcaa gatgtggaac ctggtgctcc atccatggga gccaaaagcc tttgcattcc
     4621 tttcaaccct ctgtgtgagc tgcagcccgg agccatgtgt gtctgtggca agaatcctgc
     4681 caagttctac accttgtttg gtcggagtta ctgatggaca aaacagaagc cactctctct
     4741 ctcgctcatc tttttttaaa accactgata cgaagatctt ctcagatgca atttttcttt
     4801 ttcatttttt aaaacttcta aaaaaggtca tgagataaaa cagctattct tatgcctaaa
     4861 ttaacagtgg aaaatacttt tctgtaaaca gccagggtaa taaaaaccat gaactaattg
     4921 tatttta
//
