LOCUS       NM_001412354            2611 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus epsin 1 (Epn1), transcript variant 21, mRNA.
ACCESSION   NM_001412354
VERSION     NM_001412354.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Dong,Y., Wang,B., Cui,K., Cai,X., Bhattacharjee,S., Wong,S.,
            Cowan,D.B. and Chen,H.
  TITLE     Epsins Negatively Regulate Aortic Endothelial Cell Function by
            Augmenting Inflammatory Signaling
  JOURNAL   Cells 10 (8), 1918 (2021)
   PUBMED   34440686
  REMARK    GeneRIF: Epsins Negatively Regulate Aortic Endothelial Cell
            Function by Augmenting Inflammatory Signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Song,K., Cai,X., Dong,Y., Wu,H., Wei,Y., Shankavaram,U.T., Cui,K.,
            Lee,Y., Zhu,B., Bhattacharjee,S., Wang,B., Zhang,K., Wen,A.,
            Wong,S., Yu,L., Xia,L., Welm,A.L., Bielenberg,D.R.,
            Camphausen,K.A., Kang,Y. and Chen,H.
  TITLE     Epsins 1 and 2 promote NEMO linear ubiquitination via LUBAC to
            drive breast cancer development
  JOURNAL   J Clin Invest 131 (1) (2021)
   PUBMED   32960814
  REMARK    GeneRIF: Epsins 1 and 2 promote NEMO linear ubiquitination via
            LUBAC to drive breast cancer development.
REFERENCE   3  (bases 1 to 2611)
  AUTHORS   Dong,Y., Lee,Y., Cui,K., He,M., Wang,B., Bhattacharjee,S., Zhu,B.,
            Yago,T., Zhang,K., Deng,L., Ouyang,K., Wen,A., Cowan,D.B., Song,K.,
            Yu,L., Brophy,M.L., Liu,X., Wylie-Sears,J., Wu,H., Wong,S., Cui,G.,
            Kawashima,Y., Matsumoto,H., Kodera,Y., Wojcikiewicz,R.J.H.,
            Srivastava,S., Bischoff,J., Wang,D.Z., Ley,K. and Chen,H.
  TITLE     Epsin-mediated degradation of IP3R1 fuels atherosclerosis
  JOURNAL   Nat Commun 11 (1), 3984 (2020)
   PUBMED   32770009
  REMARK    GeneRIF: Epsin-mediated degradation of IP3R1 fuels atherosclerosis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2611)
  AUTHORS   Milesi,C., Alberici,P., Pozzi,B., Oldani,A., Beznoussenko,G.V.,
            Raimondi,A., Soppo,B.E., Amodio,S., Caldieri,G., Malabarba,M.G.,
            Bertalot,G., Confalonieri,S., Parazzoli,D., Mironov,A.A.,
            Tacchetti,C., Di Fiore,P.P., Sigismund,S. and Offenhauser,N.
  TITLE     Redundant and nonredundant organismal functions of EPS15 and
            EPS15L1
  JOURNAL   Life Sci Alliance 2 (1), e201800273 (2019)
   PUBMED   30692166
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2611)
  AUTHORS   Wu,H., Rahman,H.N.A., Dong,Y., Liu,X., Lee,Y., Wen,A., To,K.H.,
            Xiao,L., Birsner,A.E., Bazinet,L., Wong,S., Song,K., Brophy,M.L.,
            Mahamud,M.R., Chang,B., Cai,X., Pasula,S., Kwak,S., Yang,W.,
            Bischoff,J., Xu,J., Bielenberg,D.R., Dixon,J.B., D'Amato,R.J.,
            Srinivasan,R.S. and Chen,H.
  TITLE     Epsin deficiency promotes lymphangiogenesis through regulation of
            VEGFR3 degradation in diabetes
  JOURNAL   J Clin Invest 128 (9), 4025-4043 (2018)
   PUBMED   30102256
  REMARK    GeneRIF: Diabetes attenuated VEGF-C-induced lymphangiogenesis in
            corneal micropocket and Matrigel plug assays in WT mice but not in
            mice with inducible lymphatic-specific deficiency of epsins 1 and 2
            (LEC-iDKO).
REFERENCE   6  (bases 1 to 2611)
  AUTHORS   Pasula,S., Cai,X., Dong,Y., Messa,M., McManus,J., Chang,B., Liu,X.,
            Zhu,H., Mansat,R.S., Yoon,S.J., Hahn,S., Keeling,J., Saunders,D.,
            Ko,G., Knight,J., Newton,G., Luscinskas,F., Sun,X., Towner,R.,
            Lupu,F., Xia,L., Cremona,O., De Camilli,P., Min,W. and Chen,H.
  TITLE     Endothelial epsin deficiency decreases tumor growth by enhancing
            VEGF signaling
  JOURNAL   J Clin Invest 122 (12), 4424-4438 (2012)
   PUBMED   23187125
  REMARK    GeneRIF: Loss of epsins 1 and 2 impaired endocytosis and
            degradation of VEGFR2, resulting in excessive VEGF signaling and
            compromised tumor vascular function.
REFERENCE   7  (bases 1 to 2611)
  AUTHORS   Chen,H., Ko,G., Zatti,A., Di Giacomo,G., Liu,L., Raiteri,E.,
            Perucco,E., Collesi,C., Min,W., Zeiss,C., De Camilli,P. and
            Cremona,O.
  TITLE     Embryonic arrest at midgestation and disruption of Notch signaling
            produced by the absence of both epsin 1 and epsin 2 in mice
  JOURNAL   Proc Natl Acad Sci U S A 106 (33), 13838-13843 (2009)
   PUBMED   19666558
  REMARK    GeneRIF: epsin has a role as a specialized endocytic adaptor, with
            a critical role in the activation of Notch signaling in mammals
REFERENCE   8  (bases 1 to 2611)
  AUTHORS   Wang,H., Traub,L.M., Weixel,K.M., Hawryluk,M.J., Shah,N.,
            Edinger,R.S., Perry,C.J., Kester,L., Butterworth,M.B., Peters,K.W.,
            Kleyman,T.R., Frizzell,R.A. and Johnson,J.P.
  TITLE     Clathrin-mediated endocytosis of the epithelial sodium channel.
            Role of epsin
  JOURNAL   J Biol Chem 281 (20), 14129-14135 (2006)
   PUBMED   16574660
  REMARK    GeneRIF: epsin is an accessory protein linking ENaC to the
            clathrin-based endocytic machinery thereby regulating the activity
            of this ion channel at the cell surface
REFERENCE   9  (bases 1 to 2611)
  AUTHORS   Chen,H., Slepnev,V.I., Di Fiore,P.P. and De Camilli,P.
  TITLE     The interaction of epsin and Eps15 with the clathrin adaptor AP-2
            is inhibited by mitotic phosphorylation and enhanced by
            stimulation-dependent dephosphorylation in nerve terminals
  JOURNAL   J Biol Chem 274 (6), 3257-3260 (1999)
   PUBMED   9920862
REFERENCE   10 (bases 1 to 2611)
  AUTHORS   Yamabhai,M., Hoffman,N.G., Hardison,N.L., McPherson,P.S.,
            Castagnoli,L., Cesareni,G. and Kay,B.K.
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and
            five Src homology 3 domains
  JOURNAL   J Biol Chem 273 (47), 31401-31407 (1998)
   PUBMED   9813051
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162893.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422590.446473.1,
                                           SRR13422597.814929.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-390               AC162893.6         47117-47506
            391-710             AC162893.6         50531-50850
            711-960             AC162893.6         56787-57036
            961-1085            AC162893.6         59709-59833
            1086-1160           AC162893.6         60180-60254
            1161-1244           AC162893.6         60767-60850
            1245-1548           AC162893.6         61822-62125
            1549-1659           AC162893.6         62511-62621
            1660-1746           AC162893.6         62756-62842
            1747-2004           AC162893.6         64022-64279
            2005-2611           AC162893.6         64440-65046
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 2.94 cM"
     gene            1..2611
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /note="epsin 1"
                     /db_xref="GeneID:13854"
                     /db_xref="MGI:MGI:1333763"
     exon            1..390
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..2213
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /note="isoform 3 is encoded by transcript variant 21;
                     EPS-15-interacting protein 1; intersectin-EH-binding
                     protein 1"
                     /codon_start=1
                     /product="epsin-1 isoform 3"
                     /protein_id="NP_001399283.1"
                     /db_xref="GeneID:13854"
                     /db_xref="MGI:MGI:1333763"
                     /translation="MLASVAASPVFRPGPWRARVQPASEPVFQPLMACGCFCTAVPGS
                     GLPADVVIYQRPLRSPFVSRVISPCPSPLTPLLGTMSTSSLRRQMKNIVHNYSEAEIK
                     VREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLM
                     EYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDR
                     LREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKE
                     EADQPPSCGPEDDVQLQLALSLSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEES
                     SLMDLADVFTTPAPPQASDPWGGPASVPTAVPVAAAASDPWGGPAVPPAADPWGGAAP
                     TPASGDPWRPAAPTGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGAPVSGPPSSDPWAP
                     APAFSDPWGGSPAKPSSNGTAAVGGFDTEPDEFSDFDRLRTALPTSGSSTGELELLAG
                     EVPARSPGAFDMSGVGGSLAESVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSL
                     VSRPGPTPPGSKASNPFLPSGAPPTGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAP
                     PTYISPLGGGPGLPPMMPPGPPAPNTNPFLL"
     exon            391..710
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            711..960
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1085
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1160
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1244
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1548
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1659
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..1746
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..2004
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..2611
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2592..2597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /note="hexamer: AATAAA"
     polyA_site      2611
                     /gene="Epn1"
                     /gene_synonym="Ibp1"
                     /note="major polyA site"
ORIGIN      
        1 ggttgcttcc cgtccgctcg gcggctcccc tcccccgccc ggctctctgc gcccctcccg
       61 ggcgccgggg cggcggggcc gtcgctgtgc ggccctccgt gcgtctgtgc gtgcgcacgt
      121 gcgcacttct gcttcccgca gctcggcgcg cgccccgcga caccgaggcc gagcgggggc
      181 agggggctga ccgtcatgac ccctggaagc ccggtataag cgggccgagg tgagcgaggg
      241 gcgcaatgct ggcctccgtc gcggcaagtc cggtgttccg gcctgggccg tggcgcgcgc
      301 gggtccagcc agcctcggag ccggtgttcc agccgctgat ggcttgtggc tgcttttgta
      361 ccgctgtgcc cgggtcgggt ttgcctgccg atgtggtgat ctaccaacgt ccattgcggt
      421 ctccctttgt gagtcgcgtc atttctccat gccccagccc cttgacacca ctactgggca
      481 caatgtcgac ctcatcgctg cggcgtcaga tgaagaatat cgtccacaac tactcagagg
      541 ctgagatcaa ggtccgcgag gccacaagca atgatccctg gggcccatcc agctccctca
      601 tgtcagagat tgctgacctc acctacaatg ttgtggcctt ctccgagatc atgagcatga
      661 tctggaagcg gctcaatgac cacggcaaga actggaggca cgtctacaag gccatgacgt
      721 tgatggagta cctcatcaag actggctctg agcgtgtatc acaacagtgc aaagagaaca
      781 tgtatgctgt gcagacgctg aaggacttcc agtatgtgga ccgtgatggc aaggaccagg
      841 gtgtgaatgt gcgggagaag gccaagcagc tggtggcttt gctgcgtgat gaggatcggc
      901 tccgggagga gcgcgcccac gcgctcaaga ccaaggagaa gctggcacag actgccacgg
      961 cctcctcagc agccgtgggc tctggaccac ctcccgaggc ggagcaggca tggccacaga
     1021 gcagcgggga ggaggagttg cagctccagc tggccctggc tatgagcaag gaggaggccg
     1081 accagccccc gtcctgcggc cctgaggatg acgtccagct ccagctggcc cttagtttga
     1141 gccgagagga gcacgataag gaagagcgga tccgtcgtgg ggatgacctg aggctgcaga
     1201 tggcaataga ggagagcaag agggagactg ggggcaagga ggagtcatct ctcatggatc
     1261 ttgctgatgt cttcacaacc ccagcgcctc cacaggcctc agacccttgg gggggcccgg
     1321 catctgtgcc tactgctgtc cctgtggctg ctgccgcttc agacccttgg gggggacctg
     1381 ctgtccctcc agctgctgat ccttggggtg gtgcagcccc cacaccagcc tcaggggatc
     1441 cctggaggcc tgcagcccct acagggccct ctgttgatcc ttggggtggg accccagctc
     1501 ctgcagctgg agaggggccc acccctgacc cgtggggaag ctctgatggt ggggctcctg
     1561 tcagtgggcc cccatcttct gacccctggg cacctgcccc ggctttctca gacccctggg
     1621 gaggctcacc tgcaaagccc agcagcaacg gcactgcagc agttgggggc tttgacacag
     1681 agcctgatga gttctcagac ttcgaccgac tccgcactgc cctgccaacc tctgggagca
     1741 gcacggggga gttggagttg ctcgcaggag aggtgcctgc tcgcagccct ggggcattcg
     1801 acatgagtgg ggttggggga tctctggctg agtctgtggg gagtccccca cctgctgcaa
     1861 ccccaactcc caccccccca acacggaaga ctccggagtc attcctaggc cctaatgcag
     1921 cccttgtgga tctggactcg ctggtgagcc gaccaggccc cacaccacca ggatccaagg
     1981 cttccaaccc attccttcca agcggagctc cgcccacagg cccctctgtc accaatcctt
     2041 tccagccagc accccctgca acgctcaccc tgaaccagct ccgtctcagt cctgtgcccc
     2101 cagttcctgg agcgccaccc acctacatct ctcctcttgg tggaggccct ggcctgcccc
     2161 ccatgatgcc cccaggtccc ccagccccca acactaaccc cttcctccta taatccaggg
     2221 taggagggag gcctggttca gctggcttcc ccaatttctg ctccctggga gatcagtatt
     2281 gtgagtgcat gtgaaatggg ggggccctcc cccacagtgc ccttccccct cctggggccc
     2341 actcacacta caccctcttc ccaagtcccc accccacctc cccttgagaa aaactggaca
     2401 tggggatggg gaggggagcc ggccagagga ggaccctttt ctgtggcatt agatggggga
     2461 gggacagctg gggttccccc acccattcca cctccctcta aactcatgac aacccccaaa
     2521 tcagtgtttg agcctcctcg ttcccttggc acccctggtg aatccttggt gatgattttg
     2581 gcaacttcgg gaataaatgg caattctcat g
//
