LOCUS       NM_007940               2062 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus epoxide hydrolase 2, cytoplasmic (Ephx2), transcript
            variant 1, mRNA.
ACCESSION   NM_007940
VERSION     NM_007940.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Li,X., Kempf,S., Delgado Lagos,F., Ukan,U., Popp,R., Hu,J.,
            Fromel,T., Gunther,S., Weigert,A. and Fleming,I.
  TITLE     A regulatory loop involving the cytochrome P450-soluble epoxide
            hydrolase axis and TGF-beta signaling
  JOURNAL   iScience 27 (10), 110938 (2024)
   PUBMED   39398242
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Yousef,A., Sosnowski,D.K., Fang,L., Legaspi,R.J., Korodimas,J.,
            Lee,A., Magor,K.E. and Seubert,J.M.
  TITLE     Cardioprotective response and senescence in aged sEH null female
            mice exposed to LPS
  JOURNAL   Am J Physiol Heart Circ Physiol 326 (6), H1366-H1385 (2024)
   PUBMED   38578240
REFERENCE   3  (bases 1 to 2062)
  AUTHORS   Naeem,Z., Zukunft,S., Huard,A., Hu,J., Hammock,B.D., Weigert,A.,
            Fromel,T. and Fleming,I.
  TITLE     Role of the soluble epoxide hydrolase in keratinocyte proliferation
            and sensitivity of skin to inflammatory stimuli
  JOURNAL   Biomed Pharmacother 171, 116127 (2024)
   PUBMED   38198951
  REMARK    GeneRIF: Role of the soluble epoxide hydrolase in keratinocyte
            proliferation and sensitivity of skin to inflammatory stimuli.
REFERENCE   4  (bases 1 to 2062)
  AUTHORS   Oguro,A., Fujiyama,T., Ishihara,Y., Kataoka,C., Yamamoto,M.,
            Eto,K., Komohara,Y., Imaoka,S., Sakuragi,T., Tsuji,M., Shibata,E.,
            Kotake,Y. and Yamazaki,T.
  TITLE     Maternal DHA intake in mice increased DHA metabolites in the pup
            brain and ameliorated MeHg-induced behavioral disorder
  JOURNAL   J Lipid Res 64 (11), 100458 (2023)
   PUBMED   37838304
REFERENCE   5  (bases 1 to 2062)
  AUTHORS   Wu,Y., Dong,J.H., Dai,Y.F., Zhu,M.Z., Wang,M.Y., Zhang,Y.,
            Pan,Y.D., Yuan,X.R., Guo,Z.X., Wang,C.X., Li,Y.Q. and Zhu,X.H.
  TITLE     Hepatic soluble epoxide hydrolase activity regulates cerebral Abeta
            metabolism and the pathogenesis of Alzheimer's disease in mice
  JOURNAL   Neuron 111 (18), 2847-2862 (2023)
   PUBMED   37402372
  REMARK    GeneRIF: Hepatic soluble epoxide hydrolase activity regulates
            cerebral Abeta metabolism and the pathogenesis of Alzheimer's
            disease in mice.
REFERENCE   6  (bases 1 to 2062)
  AUTHORS   Marcus,R.C., Gale,N.W., Morrison,M.E., Mason,C.A. and
            Yancopoulos,G.D.
  TITLE     Eph family receptors and their ligands distribute in opposing
            gradients in the developing mouse retina
  JOURNAL   Dev Biol 180 (2), 786-789 (1996)
   PUBMED   8954746
REFERENCE   7  (bases 1 to 2062)
  AUTHORS   Johansson,C., Stark,A., Sandberg,M., Ek,B., Rask,L. and Meijer,J.
  TITLE     Tissue specific basal expression of soluble murine epoxide
            hydrolase and effects of clofibrate on the mRNA levels in
            extrahepatic tissues and liver
  JOURNAL   Arch Toxicol 70 (1), 61-63 (1995)
   PUBMED   8750907
REFERENCE   8  (bases 1 to 2062)
  AUTHORS   Grant,D.F., Spearow,J.L., Storms,D.H., Edelhoff,S., Adler,D.A.,
            Disteche,C.M., Taylor,B.A. and Hammock,B.D.
  TITLE     Chromosomal mapping and expression levels of a mouse soluble
            epoxide hydrolase gene
  JOURNAL   Pharmacogenetics 4 (2), 64-72 (1994)
   PUBMED   7915936
REFERENCE   9  (bases 1 to 2062)
  AUTHORS   Grant,D.F., Storms,D.H. and Hammock,B.D.
  TITLE     Molecular cloning and expression of murine liver soluble epoxide
            hydrolase
  JOURNAL   J Biol Chem 268 (23), 17628-17633 (1993)
   PUBMED   8349642
REFERENCE   10 (bases 1 to 2062)
  AUTHORS   Simmons,D.L., Lalley,P.A. and Kasper,C.B.
  TITLE     Chromosomal assignments of genes coding for components of the
            mixed-function oxidase system in mice. Genetic localization of the
            cytochrome P-450PCN and P-450PB gene families and the
            nadph-cytochrome P-450 oxidoreductase and epoxide hydratase genes
  JOURNAL   J Biol Chem 260 (1), 515-521 (1985)
   PUBMED   3917435
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC126272.3 and AK142714.1.
            
            On Sep 22, 2012 this sequence version replaced NM_007940.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142714.1, Z37107.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AC126272.3         155101-155129       c
            30-577              AK142714.1         3-550
            578-578             AC126272.3         138630-138630       c
            579-2050            AK142714.1         552-2023
            2051-2062           AC126272.3         114979-114990       c
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 34.36 cM"
     gene            1..2062
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="epoxide hydrolase 2, cytoplasmic"
                     /db_xref="GeneID:13850"
                     /db_xref="MGI:MGI:99500"
     exon            1..188
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1752
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /EC_number="3.3.2.10"
                     /EC_number="3.1.3.76"
                     /note="isoform a is encoded by transcript variant 1;
                     epoxide hydrolase 2C; epoxide hydratase; soluble epoxide
                     hydrolase; cytosolic epoxide hydrolase; bifunctional
                     epoxide hydrolase 2"
                     /codon_start=1
                     /product="bifunctional epoxide hydrolase 2 isoform a"
                     /protein_id="NP_031966.2"
                     /db_xref="CCDS:CCDS27218.1"
                     /db_xref="GeneID:13850"
                     /db_xref="MGI:MGI:99500"
                     /translation="MALRVAAFDLDGVLALPSIAGAFRRSEEALALPRDFLLGAYQTE
                     FPEGPTEQLMKGKITFSQWVPLMDESYRKSSKACGANLPENFSISQIFSQAMAARSIN
                     RPMLQAAIALKKKGFTTCIVTNNWLDDGDKRDSLAQMMCELSQHFDFLIESCQVGMIK
                     PEPQIYNFLLDTLKAKPNEVVFLDDFGSNLKPARDMGMVTILVHNTASALRELEKVTG
                     TQFPEAPLPVPCNPNDVSHGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFSWRYQI
                     PALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDW
                     AGVMVWNMALFYPERVRAVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAE
                     AELEKNMSRTFKSFFRASDETGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYI
                     QQFKKTGFRGPLNWYRNTERNWKWSCKGLGRKILVPALMVTAEKDIVLRPEMSKNMEK
                     WIPFLKRGHIEDCGHWTQIEKPTEVNQILIKWLQTEVQNPSVTSKI"
     exon            189..273
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            274..433
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            434..624
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            625..747
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            748..816
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            817..912
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            913..991
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1026
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1053
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1139
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1251
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1326
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1360
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1463
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1533
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1614
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1673
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..2062
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2037..2042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="hexamer: AATAAA"
     polyA_site      2061
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="major polyA site"
ORIGIN      
        1 taggttccca cagccagcct ccccactcta ggccacagcc ttccagcttc gtgtctgtgt
       61 cagcttgacg ctgcagcccg ggccgccatg gcgctgcgtg tagccgcgtt cgaccttgac
      121 ggagtgctgg ccctcccctc tatcgccggg gctttccgcc gcagcgaaga ggccctggca
      181 ctgcctagag acttcctgct tggtgcgtac cagacggaat tcccagaggg acccactgag
      241 caactcatga aagggaagat cacattttcg cagtgggtac cactcatgga tgaaagctac
      301 aggaagtcct ccaaagcctg tggagccaat ctacctgaga atttctccat aagtcaaata
      361 ttcagccaag ctatggcagc aagaagcatc aaccgcccca tgcttcaggc agccattgct
      421 ctcaaaaaga aaggattcac aacatgcatt gtcaccaaca actggctgga cgacggagac
      481 aagagagaca gcctggccca gatgatgtgt gagctgagcc aacactttga cttcctgata
      541 gagtcctgtc aggttgggat gatcaagcct gagcctcaga tctacaattt tttactggat
      601 accctgaagg caaaacccaa tgaggttgtt ttcctagatg actttggaag taatctgaag
      661 ccagcccgtg acatggggat ggttaccatc ctggtccaca acacagcctc cgctctgaga
      721 gaactggaga aggtcacagg gacacagttt cctgaggccc cactgccagt cccatgcaat
      781 ccaaatgacg tcagccatgg atatgtgaca gtgaagccag ggatccgcct gcattttgtg
      841 gagatgggct ctggccctgc cctatgcctt tgccatgggt ttcctgagag ctggttttct
      901 tggaggtacc agatccctgc tctggcccag gcaggctttc gtgttctggc tatagacatg
      961 aaaggctatg gagactcatc ttctcctcct gaaatagaag aatatgccat ggaattgctg
     1021 tgtaaggaga tggtgacatt cctggataag ctgggaatcc ctcaagcagt gttcattggc
     1081 catgactggg ctggtgtgat ggtgtggaac atggctctct tctaccctga gagagtgagg
     1141 gctgtggcca gtttgaacac gccgtttatg ccaccagatc ctgatgtgtc tcccatgaaa
     1201 gttatccgat ctatcccagt tttcaattat cagctgtact ttcaagaacc aggagtggcc
     1261 gaggctgaac tggagaagaa catgagtcgg actttcaaaa gcttcttccg agccagtgat
     1321 gagacaggtt tcatcgctgt gcataaagcc actgaaatag ggggaatcct tgtgaatact
     1381 ccagaagatc ccaacctcag caaaattact actgaggaag aaatagagtt ttacatacag
     1441 cagttcaaga agactggctt cagaggtcct ctgaactggt accggaacac agaaagaaac
     1501 tggaagtgga gctgtaaagg gttgggacga aagatcttgg tcccagccct gatggtcaca
     1561 gctgagaagg acattgtact ccgtcctgaa atgtccaaga acatggaaaa gtggatccct
     1621 ttcctgaaaa ggggacacat tgaagactgt ggtcactgga cacagataga gaaaccaact
     1681 gaggtgaacc agattctcat caagtggctg cagactgaag tccagaaccc atcagtgacc
     1741 tccaagattt agccactggg gacacatttt agtttctgga acacagcctg atctacaagt
     1801 accagcatcg ttcttttgcc agccagtgat tttcttttaa atgaaaatga tgggatgaga
     1861 tgtaatttta gatcgggaag agagtgtgtg tctaattctt ttgagtatgc ctgtgccatc
     1921 agagaagaga tcccacccca gtaggaaggt atggggcagt ccagtttata actttgcaac
     1981 caaacccaag cctgctcttt ttgaagcagc tgattggaga gtaaagattt tcattcaata
     2041 aagctaaacc tcagggctcc ag
//
