LOCUS       NM_001271403            1987 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus epoxide hydrolase 2, cytoplasmic (Ephx2), transcript
            variant 3, mRNA.
ACCESSION   NM_001271403
VERSION     NM_001271403.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Li,X., Kempf,S., Delgado Lagos,F., Ukan,U., Popp,R., Hu,J.,
            Fromel,T., Gunther,S., Weigert,A. and Fleming,I.
  TITLE     A regulatory loop involving the cytochrome P450-soluble epoxide
            hydrolase axis and TGF-beta signaling
  JOURNAL   iScience 27 (10), 110938 (2024)
   PUBMED   39398242
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Yousef,A., Sosnowski,D.K., Fang,L., Legaspi,R.J., Korodimas,J.,
            Lee,A., Magor,K.E. and Seubert,J.M.
  TITLE     Cardioprotective response and senescence in aged sEH null female
            mice exposed to LPS
  JOURNAL   Am J Physiol Heart Circ Physiol 326 (6), H1366-H1385 (2024)
   PUBMED   38578240
REFERENCE   3  (bases 1 to 1987)
  AUTHORS   Naeem,Z., Zukunft,S., Huard,A., Hu,J., Hammock,B.D., Weigert,A.,
            Fromel,T. and Fleming,I.
  TITLE     Role of the soluble epoxide hydrolase in keratinocyte proliferation
            and sensitivity of skin to inflammatory stimuli
  JOURNAL   Biomed Pharmacother 171, 116127 (2024)
   PUBMED   38198951
  REMARK    GeneRIF: Role of the soluble epoxide hydrolase in keratinocyte
            proliferation and sensitivity of skin to inflammatory stimuli.
REFERENCE   4  (bases 1 to 1987)
  AUTHORS   Oguro,A., Fujiyama,T., Ishihara,Y., Kataoka,C., Yamamoto,M.,
            Eto,K., Komohara,Y., Imaoka,S., Sakuragi,T., Tsuji,M., Shibata,E.,
            Kotake,Y. and Yamazaki,T.
  TITLE     Maternal DHA intake in mice increased DHA metabolites in the pup
            brain and ameliorated MeHg-induced behavioral disorder
  JOURNAL   J Lipid Res 64 (11), 100458 (2023)
   PUBMED   37838304
REFERENCE   5  (bases 1 to 1987)
  AUTHORS   Wu,Y., Dong,J.H., Dai,Y.F., Zhu,M.Z., Wang,M.Y., Zhang,Y.,
            Pan,Y.D., Yuan,X.R., Guo,Z.X., Wang,C.X., Li,Y.Q. and Zhu,X.H.
  TITLE     Hepatic soluble epoxide hydrolase activity regulates cerebral Abeta
            metabolism and the pathogenesis of Alzheimer's disease in mice
  JOURNAL   Neuron 111 (18), 2847-2862 (2023)
   PUBMED   37402372
  REMARK    GeneRIF: Hepatic soluble epoxide hydrolase activity regulates
            cerebral Abeta metabolism and the pathogenesis of Alzheimer's
            disease in mice.
REFERENCE   6  (bases 1 to 1987)
  AUTHORS   Marcus,R.C., Gale,N.W., Morrison,M.E., Mason,C.A. and
            Yancopoulos,G.D.
  TITLE     Eph family receptors and their ligands distribute in opposing
            gradients in the developing mouse retina
  JOURNAL   Dev Biol 180 (2), 786-789 (1996)
   PUBMED   8954746
REFERENCE   7  (bases 1 to 1987)
  AUTHORS   Johansson,C., Stark,A., Sandberg,M., Ek,B., Rask,L. and Meijer,J.
  TITLE     Tissue specific basal expression of soluble murine epoxide
            hydrolase and effects of clofibrate on the mRNA levels in
            extrahepatic tissues and liver
  JOURNAL   Arch Toxicol 70 (1), 61-63 (1995)
   PUBMED   8750907
REFERENCE   8  (bases 1 to 1987)
  AUTHORS   Grant,D.F., Spearow,J.L., Storms,D.H., Edelhoff,S., Adler,D.A.,
            Disteche,C.M., Taylor,B.A. and Hammock,B.D.
  TITLE     Chromosomal mapping and expression levels of a mouse soluble
            epoxide hydrolase gene
  JOURNAL   Pharmacogenetics 4 (2), 64-72 (1994)
   PUBMED   7915936
REFERENCE   9  (bases 1 to 1987)
  AUTHORS   Grant,D.F., Storms,D.H. and Hammock,B.D.
  TITLE     Molecular cloning and expression of murine liver soluble epoxide
            hydrolase
  JOURNAL   J Biol Chem 268 (23), 17628-17633 (1993)
   PUBMED   8349642
REFERENCE   10 (bases 1 to 1987)
  AUTHORS   Simmons,D.L., Lalley,P.A. and Kasper,C.B.
  TITLE     Chromosomal assignments of genes coding for components of the
            mixed-function oxidase system in mice. Genetic localization of the
            cytochrome P-450PCN and P-450PB gene families and the
            nadph-cytochrome P-450 oxidoreductase and epoxide hydratase genes
  JOURNAL   J Biol Chem 260 (1), 515-521 (1985)
   PUBMED   3917435
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY098585.1 and AC126272.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR, lacks
            an in-frame portion of the 5' coding region, and initiates
            translation at an alternate start codon, compared to variant 1.
            This variant is considered to be 5' complete based on evidence from
            PMID: 15601917. The encoded isoform (c) contains a distinct
            N-terminus and is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY098585.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1985              AY098585.1         15-1999
            1986-1987           AC126272.3         114979-114980       c
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 34.36 cM"
     gene            1..1987
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="epoxide hydrolase 2, cytoplasmic"
                     /db_xref="GeneID:13850"
                     /db_xref="MGI:MGI:99500"
     exon            1..198
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..1677
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /EC_number="3.3.2.10"
                     /EC_number="3.1.3.76"
                     /note="isoform c is encoded by transcript variant 3;
                     epoxide hydrolase 2C; epoxide hydratase; soluble epoxide
                     hydrolase; cytosolic epoxide hydrolase; bifunctional
                     epoxide hydrolase 2"
                     /codon_start=1
                     /product="bifunctional epoxide hydrolase 2 isoform c"
                     /protein_id="NP_001258332.1"
                     /db_xref="CCDS:CCDS88694.1"
                     /db_xref="GeneID:13850"
                     /db_xref="MGI:MGI:99500"
                     /translation="MRFAAMAAFSVFFVSKGLLMNSNIWCVGQEGPSQEDTDTIHTSE
                     WVPLMDESYRKSSKACGANLPENFSISQIFSQAMAARSINRPMLQAAIALKKKGFTTC
                     IVTNNWLDDGDKRDSLAQMMCELSQHFDFLIESCQVGMIKPEPQIYNFLLDTLKAKPN
                     EVVFLDDFGSNLKPARDMGMVTILVHNTASALRELEKVTGTQFPEAPLPVPCNPNDVS
                     HGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGY
                     GDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRA
                     VASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRAS
                     DETGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNT
                     ERNWKWSCKGLGRKILVPALMVTAEKDIVLRPEMSKNMEKWIPFLKRGHIEDCGHWTQ
                     IEKPTEVNQILIKWLQTEVQNPSVTSKI"
     exon            199..358
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            359..549
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            550..672
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            673..741
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            742..837
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            838..916
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            917..951
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            952..978
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1064
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1176
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1251
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1285
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1388
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1458
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1539
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1598
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1599..1987
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1962..1967
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="hexamer: AATAAA"
     polyA_site      1986
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="major polyA site"
ORIGIN      
        1 gggtctctcc tctggcctgc atgctgagag gagggccccg acatgttgga tcaataaaaa
       61 atcctcatgc ggtttgcagc gatggcggcg ttctctgtgt tctttgtgag taagggtctt
      121 ttgatgaact ccaacatttg gtgtgttggc caggaaggcc cttctcagga ggacacagac
      181 accatacata catcagagtg ggtaccactc atggatgaaa gctacaggaa gtcctccaaa
      241 gcctgtggag ccaatctacc tgagaatttc tccataagtc aaatattcag ccaagctatg
      301 gcagcaagaa gcatcaaccg ccccatgctt caggcagcca ttgctctcaa aaagaaagga
      361 ttcacaacat gcattgtcac caacaactgg ctggacgacg gagacaagag agacagcctg
      421 gcccagatga tgtgtgagct gagccaacac tttgacttcc tgatagagtc ctgtcaggtt
      481 gggatgatca agcctgagcc tcagatctac aattttttac tggataccct gaaggcaaaa
      541 cccaatgagg ttgttttcct agatgacttt ggaagtaatc tgaagccagc ccgtgacatg
      601 gggatggtta ccatcctggt ccacaacaca gcctccgctc tgagagaact ggagaaggtc
      661 acagggacac agtttcctga ggccccactg ccagtcccat gcaatccaaa tgacgtcagc
      721 catggatatg tgacagtgaa gccagggatc cgcctgcatt ttgtggagat gggctctggc
      781 cctgccctat gcctttgcca tgggtttcct gagagctggt tttcttggag gtaccagatc
      841 cctgctctgg cccaggcagg ctttcgtgtt ctggctatag acatgaaagg ctatggagac
      901 tcatcttctc ctcctgaaat agaagaatat gccatggaat tgctgtgtaa ggagatggtg
      961 acattcctgg ataagctggg aatccctcaa gcagtgttca ttggccatga ctgggctggt
     1021 gtgatggtgt ggaacatggc tctcttctac cctgagagag tgagggctgt ggccagtttg
     1081 aacacgccgt ttatgccacc agatcctgat gtgtctccca tgaaagttat ccgatctatc
     1141 ccagttttca attatcagct gtactttcaa gaaccaggag tggccgaggc tgaactggag
     1201 aagaacatga gtcggacttt caaaagcttc ttccgagcca gtgatgagac aggtttcatc
     1261 gctgtgcata aagccactga aataggggga atccttgtga atactccaga agatcccaac
     1321 ctcagcaaaa ttactactga ggaagaaata gagttttaca tacagcagtt caagaagact
     1381 ggcttcagag gtcctctgaa ctggtaccgg aacacagaaa gaaactggaa gtggagctgt
     1441 aaagggttgg gacgaaagat cttggtccca gccctgatgg tcacagctga gaaggacatt
     1501 gtactccgtc ctgaaatgtc caagaacatg gaaaagtgga tccctttcct gaaaagggga
     1561 cacattgaag actgtggtca ctggacacag atagagaaac caactgaggt gaaccagatt
     1621 ctcatcaagt ggctgcagac tgaagtccag aacccatcag tgacctccaa gatttagcca
     1681 ctggggacac attttagttt ctggaacaca gcctgatcta caagtaccag catcgttctt
     1741 ttgccagcca gtgattttct tttaaatgaa aatgatggga tgagatgtaa ttttagatcg
     1801 ggaagagagt gtgtgtctaa ttcttttgag tatgcctgtg ccatcagaga agagatccca
     1861 ccccagtagg aaggtatggg gcagtccagt ttataacttt gcaaccaaac ccaagcctgc
     1921 tctttttgaa gcagctgatt ggagagtaaa gattttcatt caataaagct aaacctcagg
     1981 gctccag
//
