LOCUS       NM_001271402            2142 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus epoxide hydrolase 2, cytoplasmic (Ephx2), transcript
            variant 2, mRNA.
ACCESSION   NM_001271402
VERSION     NM_001271402.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Li,X., Kempf,S., Delgado Lagos,F., Ukan,U., Popp,R., Hu,J.,
            Fromel,T., Gunther,S., Weigert,A. and Fleming,I.
  TITLE     A regulatory loop involving the cytochrome P450-soluble epoxide
            hydrolase axis and TGF-beta signaling
  JOURNAL   iScience 27 (10), 110938 (2024)
   PUBMED   39398242
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Yousef,A., Sosnowski,D.K., Fang,L., Legaspi,R.J., Korodimas,J.,
            Lee,A., Magor,K.E. and Seubert,J.M.
  TITLE     Cardioprotective response and senescence in aged sEH null female
            mice exposed to LPS
  JOURNAL   Am J Physiol Heart Circ Physiol 326 (6), H1366-H1385 (2024)
   PUBMED   38578240
REFERENCE   3  (bases 1 to 2142)
  AUTHORS   Naeem,Z., Zukunft,S., Huard,A., Hu,J., Hammock,B.D., Weigert,A.,
            Fromel,T. and Fleming,I.
  TITLE     Role of the soluble epoxide hydrolase in keratinocyte proliferation
            and sensitivity of skin to inflammatory stimuli
  JOURNAL   Biomed Pharmacother 171, 116127 (2024)
   PUBMED   38198951
  REMARK    GeneRIF: Role of the soluble epoxide hydrolase in keratinocyte
            proliferation and sensitivity of skin to inflammatory stimuli.
REFERENCE   4  (bases 1 to 2142)
  AUTHORS   Oguro,A., Fujiyama,T., Ishihara,Y., Kataoka,C., Yamamoto,M.,
            Eto,K., Komohara,Y., Imaoka,S., Sakuragi,T., Tsuji,M., Shibata,E.,
            Kotake,Y. and Yamazaki,T.
  TITLE     Maternal DHA intake in mice increased DHA metabolites in the pup
            brain and ameliorated MeHg-induced behavioral disorder
  JOURNAL   J Lipid Res 64 (11), 100458 (2023)
   PUBMED   37838304
REFERENCE   5  (bases 1 to 2142)
  AUTHORS   Wu,Y., Dong,J.H., Dai,Y.F., Zhu,M.Z., Wang,M.Y., Zhang,Y.,
            Pan,Y.D., Yuan,X.R., Guo,Z.X., Wang,C.X., Li,Y.Q. and Zhu,X.H.
  TITLE     Hepatic soluble epoxide hydrolase activity regulates cerebral Abeta
            metabolism and the pathogenesis of Alzheimer's disease in mice
  JOURNAL   Neuron 111 (18), 2847-2862 (2023)
   PUBMED   37402372
  REMARK    GeneRIF: Hepatic soluble epoxide hydrolase activity regulates
            cerebral Abeta metabolism and the pathogenesis of Alzheimer's
            disease in mice.
REFERENCE   6  (bases 1 to 2142)
  AUTHORS   Marcus,R.C., Gale,N.W., Morrison,M.E., Mason,C.A. and
            Yancopoulos,G.D.
  TITLE     Eph family receptors and their ligands distribute in opposing
            gradients in the developing mouse retina
  JOURNAL   Dev Biol 180 (2), 786-789 (1996)
   PUBMED   8954746
REFERENCE   7  (bases 1 to 2142)
  AUTHORS   Johansson,C., Stark,A., Sandberg,M., Ek,B., Rask,L. and Meijer,J.
  TITLE     Tissue specific basal expression of soluble murine epoxide
            hydrolase and effects of clofibrate on the mRNA levels in
            extrahepatic tissues and liver
  JOURNAL   Arch Toxicol 70 (1), 61-63 (1995)
   PUBMED   8750907
REFERENCE   8  (bases 1 to 2142)
  AUTHORS   Grant,D.F., Spearow,J.L., Storms,D.H., Edelhoff,S., Adler,D.A.,
            Disteche,C.M., Taylor,B.A. and Hammock,B.D.
  TITLE     Chromosomal mapping and expression levels of a mouse soluble
            epoxide hydrolase gene
  JOURNAL   Pharmacogenetics 4 (2), 64-72 (1994)
   PUBMED   7915936
REFERENCE   9  (bases 1 to 2142)
  AUTHORS   Grant,D.F., Storms,D.H. and Hammock,B.D.
  TITLE     Molecular cloning and expression of murine liver soluble epoxide
            hydrolase
  JOURNAL   J Biol Chem 268 (23), 17628-17633 (1993)
   PUBMED   8349642
REFERENCE   10 (bases 1 to 2142)
  AUTHORS   Simmons,D.L., Lalley,P.A. and Kasper,C.B.
  TITLE     Chromosomal assignments of genes coding for components of the
            mixed-function oxidase system in mice. Genetic localization of the
            cytochrome P-450PCN and P-450PB gene families and the
            nadph-cytochrome P-450 oxidoreductase and epoxide hydratase genes
  JOURNAL   J Biol Chem 260 (1), 515-521 (1985)
   PUBMED   3917435
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC126272.3 and AK002415.1.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            at the 5' exon, differs in the 5' UTR, and initiates translation at
            a downstream in-frame start codon, compared to variant 1. The
            encoded isoform (b) has a shorter N-terminus, compared to isoform
            a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK002415.1, SRR13422591.145763.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC126272.3         155100-155129       c
            31-2125             AK002415.1         1-2095
            2126-2142           AC126272.3         114979-114995       c
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 34.36 cM"
     gene            1..2142
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="epoxide hydrolase 2, cytoplasmic"
                     /db_xref="GeneID:13850"
                     /db_xref="MGI:MGI:99500"
     exon            1..268
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            269..353
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     CDS             327..1832
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /EC_number="3.3.2.10"
                     /EC_number="3.1.3.76"
                     /note="isoform b is encoded by transcript variant 2;
                     epoxide hydrolase 2C; epoxide hydratase; soluble epoxide
                     hydrolase; cytosolic epoxide hydrolase; bifunctional
                     epoxide hydrolase 2"
                     /codon_start=1
                     /product="bifunctional epoxide hydrolase 2 isoform b"
                     /protein_id="NP_001258331.1"
                     /db_xref="GeneID:13850"
                     /db_xref="MGI:MGI:99500"
                     /translation="MKGKITFSQWVPLMDESYRKSSKACGANLPENFSISQIFSQAMA
                     ARSINRPMLQAAIALKKKGFTTCIVTNNWLDDGDKRDSLAQMMCELSQHFDFLIESCQ
                     VGMIKPEPQIYNFLLDTLKAKPNEVVFLDDFGSNLKPARDMGMVTILVHNTASALREL
                     EKVTGTQFPEAPLPVPCNPNDVSHGYVTVKPGIRLHFVEMGSGPALCLCHGFPESWFS
                     WRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVF
                     IGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQE
                     PGVAEAELEKNMSRTFKSFFRASDETGFIAVHKATEIGGILVNTPEDPNLSKITTEEE
                     IEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRKILVPALMVTAEKDIVLRPEMS
                     KNMEKWIPFLKRGHIEDCGHWTQIEKPTEVNQILIKWLQTEVQNPSVTSKI"
     exon            354..513
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            514..704
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            705..827
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            828..896
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            897..992
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1071
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1106
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1133
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1219
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1331
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1406
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1440
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1543
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1613
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1694
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1753
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..2142
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2117..2122
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="hexamer: AATAAA"
     polyA_site      2141
                     /gene="Ephx2"
                     /gene_synonym="CEH; Eph2; SEH; sEP"
                     /note="major polyA site"
ORIGIN      
        1 taggttccca cagccagcct ccccactcta ggccacagcc ttccagcttc gtgtctgtgt
       61 cagcttgacg ctgcagcccg ggccgccatg gcgctgcgtg tagccgcgtt cgaccttgac
      121 ggagtgctgg ccctcccctc tatcgccggg gctttccgcc gcagcgaaga ggccctggca
      181 ctgcctaggt aaggggagcc gcgctgggtc agtgtcccgc ctggtgccct gtccatacgt
      241 ccagcccagg tttcaaattg ctttccagag acttcctgct tggtgcgtac cagacggaat
      301 tcccagaggg acccactgag caactcatga aagggaagat cacattttcg cagtgggtac
      361 cactcatgga tgaaagctac aggaagtcct ccaaagcctg tggagccaat ctacctgaga
      421 atttctccat aagtcaaata ttcagccaag ctatggcagc aagaagcatc aaccgcccca
      481 tgcttcaggc agccattgct ctcaaaaaga aaggattcac aacatgcatt gtcaccaaca
      541 actggctgga cgacggagac aagagagaca gcctggccca gatgatgtgt gagctgagcc
      601 aacactttga cttcctgata gagtcctgtc aggttgggat gatcaagcct gagcctcaga
      661 tctacaattt tttactggat accctgaagg caaaacccaa tgaggttgtt ttcctagatg
      721 actttggaag taatctgaag ccagcccgtg acatggggat ggttaccatc ctggtccaca
      781 acacagcctc cgctctgaga gaactggaga aggtcacagg gacacagttt cctgaggccc
      841 cactgccagt cccatgcaat ccaaatgacg tcagccatgg atatgtgaca gtgaagccag
      901 ggatccgcct gcattttgtg gagatgggct ctggccctgc cctatgcctt tgccatgggt
      961 ttcctgagag ctggttttct tggaggtacc agatccctgc tctggcccag gcaggctttc
     1021 gtgttctggc tatagacatg aaaggctatg gagactcatc ttctcctcct gaaatagaag
     1081 aatatgccat ggaattgctg tgtaaggaga tggtgacatt cctggataag ctgggaatcc
     1141 ctcaagcagt gttcattggc catgactggg ctggtgtgat ggtgtggaac atggctctct
     1201 tctaccctga gagagtgagg gctgtggcca gtttgaacac gccgtttatg ccaccagatc
     1261 ctgatgtgtc tcccatgaaa gttatccgat ctatcccagt tttcaattat cagctgtact
     1321 ttcaagaacc aggagtggcc gaggctgaac tggagaagaa catgagtcgg actttcaaaa
     1381 gcttcttccg agccagtgat gagacaggtt tcatcgctgt gcataaagcc actgaaatag
     1441 ggggaatcct tgtgaatact ccagaagatc ccaacctcag caaaattact actgaggaag
     1501 aaatagagtt ttacatacag cagttcaaga agactggctt cagaggtcct ctgaactggt
     1561 accggaacac agaaagaaac tggaagtgga gctgtaaagg gttgggacga aagatcttgg
     1621 tcccagccct gatggtcaca gctgagaagg acattgtact ccgtcctgaa atgtccaaga
     1681 acatggaaaa gtggatccct ttcctgaaaa ggggacacat tgaagactgt ggtcactgga
     1741 cacagataga gaaaccaact gaggtgaacc agattctcat caagtggctg cagactgaag
     1801 tccagaaccc atcagtgacc tccaagattt agccactggg gacacatttt agtttctgga
     1861 acacagcctg atctacaagt accagcatcg ttcttttgcc agccagtgat tttcttttaa
     1921 atgaaaatga tgggatgaga tgtaatttta gatcgggaag agagtgtgtg tctaattctt
     1981 ttgagtatgc ctgtgccatc agagaagaga tcccacccca gtaggaaggt atggggcagt
     2041 ccagtttata actttgcaac caaacccaag cctgctcttt ttgaagcagc tgattggaga
     2101 gtaaagattt tcattcaata aagctaaacc tcagggctcc ag
//
