LOCUS       NM_010145               1754 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus epoxide hydrolase 1, microsomal (Ephx1), transcript
            variant 1, mRNA.
ACCESSION   NM_010145
VERSION     NM_010145.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Cheng,Y.S., Zhang,T., Ma,X., Pratuangtham,S., Zhang,G.C.,
            Ondrus,A.A., Mafi,A., Lomenick,B., Jones,J.J. and Ondrus,A.E.
  TITLE     A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell
            membranes
  JOURNAL   Nat Chem Biol 17 (12), 1271-1280 (2021)
   PUBMED   34799735
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Jamieson,K.L., Darwesh,A.M., Sosnowski,D.K., Zhang,H., Shah,S.,
            Zhabyeyev,P., Yang,J., Hammock,B.D., Edin,M.L., Zeldin,D.C.,
            Oudit,G.Y., Kassiri,Z. and Seubert,J.M.
  TITLE     Soluble Epoxide Hydrolase in Aged Female Mice and Human Explanted
            Hearts Following Ischemic Injury
  JOURNAL   Int J Mol Sci 22 (4), 1691 (2021)
   PUBMED   33567578
  REMARK    GeneRIF: Soluble Epoxide Hydrolase in Aged Female Mice and Human
            Explanted Hearts Following Ischemic Injury.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1754)
  AUTHORS   Edin,M.L., Yamanashi,H., Boeglin,W.E., Graves,J.P., DeGraff,L.M.,
            Lih,F.B., Zeldin,D.C. and Brash,A.R.
  TITLE     Epoxide hydrolase 3 (Ephx3) gene disruption reduces ceramide
            linoleate epoxide hydrolysis and impairs skin barrier function
  JOURNAL   J Biol Chem 296, 100198 (2021)
   PUBMED   33334892
REFERENCE   4  (bases 1 to 1754)
  AUTHORS   Cen,C., Chen,M., Zhou,J., Zhang,L., Duo,S., Jiang,L., Hou,X. and
            Gao,F.
  TITLE     Inactivation of Wt1 causes pre-granulosa cell to steroidogenic cell
            transformation and defect of ovary developmentdagger
  JOURNAL   Biol Reprod 103 (1), 60-69 (2020)
   PUBMED   32301970
REFERENCE   5  (bases 1 to 1754)
  AUTHORS   Wang,W., Yang,J., Zhang,J., Wang,Y., Hwang,S.H., Qi,W., Wan,D.,
            Kim,D., Sun,J., Sanidad,K.Z., Yang,H., Park,Y., Liu,J.Y., Zhao,X.,
            Zheng,X., Liu,Z., Hammock,B.D. and Zhang,G.
  TITLE     Lipidomic profiling reveals soluble epoxide hydrolase as a
            therapeutic target of obesity-induced colonic inflammation
  JOURNAL   Proc Natl Acad Sci U S A 115 (20), 5283-5288 (2018)
   PUBMED   29717038
  REMARK    GeneRIF: High epoxide hydrolase expression is associated with
            colonic inflammation.
REFERENCE   6  (bases 1 to 1754)
  AUTHORS   Huppi,K., Bhatia,K., Siwarski,D., Klinman,D., Cherney,B. and
            Smulson,M.
  TITLE     Sequence and organization of the mouse poly (ADP-ribose) polymerase
            gene
  JOURNAL   Nucleic Acids Res 17 (9), 3387-3401 (1989)
   PUBMED   2498841
REFERENCE   7  (bases 1 to 1754)
  AUTHORS   Skoda,R.C., Demierre,A., McBride,O.W., Gonzalez,F.J. and Meyer,U.A.
  TITLE     Human microsomal xenobiotic epoxide hydrolase. Complementary DNA
            sequence, complementary DNA-directed expression in COS-1 cells, and
            chromosomal localization
  JOURNAL   J Biol Chem 263 (3), 1549-1554 (1988)
   PUBMED   2891713
REFERENCE   8  (bases 1 to 1754)
  AUTHORS   Simmons,D.L., Lalley,P.A. and Kasper,C.B.
  TITLE     Chromosomal assignments of genes coding for components of the
            mixed-function oxidase system in mice. Genetic localization of the
            cytochrome P-450PCN and P-450PB gene families and the
            nadph-cytochrome P-450 oxidoreductase and epoxide hydratase genes
  JOURNAL   J Biol Chem 260 (1), 515-521 (1985)
   PUBMED   3917435
REFERENCE   9  (bases 1 to 1754)
  AUTHORS   Traina,V.L., Taylor,B.A. and Cohen,J.C.
  TITLE     Genetic mapping of endogenous mouse mammary tumor viruses: locus
            characterization, segregation, and chromosomal distribution
  JOURNAL   J Virol 40 (3), 735-744 (1981)
   PUBMED   6275115
REFERENCE   10 (bases 1 to 1754)
  AUTHORS   Gonzalez,F.J. and Kasper,C.B.
  TITLE     Cloning of epoxide hydratase complementary DNA
  JOURNAL   J Biol Chem 256 (10), 4697-4700 (1981)
   PUBMED   6262298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117673.18, BY312366.1, AK018249.1 and AW047588.1.
            
            On Aug 25, 2015 this sequence version replaced NM_010145.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061493.1, AK018249.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC117673.18        167954-167977       c
            25-73               BY312366.1         25-73
            74-1375             AK018249.1         9-1310
            1376-1754           AW047588.1         1-379               c
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 84.48 cM"
     gene            1..1754
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /note="epoxide hydrolase 1, microsomal"
                     /db_xref="GeneID:13849"
                     /db_xref="MGI:MGI:95405"
     exon            1..143
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            144..331
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     CDS             149..1516
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /EC_number="3.3.2.9"
                     /note="isoform 1 is encoded by transcript variant 1;
                     epoxide hydratase; microsomal epoxide hydrolase"
                     /codon_start=1
                     /product="epoxide hydrolase 1 isoform 1"
                     /protein_id="NP_034275.1"
                     /db_xref="CCDS:CCDS15579.1"
                     /db_xref="GeneID:13849"
                     /db_xref="MGI:MGI:95405"
                     /translation="MWLELILASVLGFVIYWFVSRDKEETLPLEDGWWGPGSKPSAKE
                     DESIRPFKVETSDEEIKDLHQRIDRFRASPPLEGSRFHYGFNSSYLKKVVSFWRNEFD
                     WRKQVEILNQYPHFKTKIEGLDIHFIHVKPPQLPSGRTPKPLLMVHGWPGSFYEFYKI
                     IPLLTDPKTHGLSDEHVFEVICPSIPGYGFSEASSKKGLNSVATARIFYKLMSRLGFQ
                     KFYIQGGDWGSLICTNIAQMVPNHVKGLHLNMSFISRNIYSLTPLLGQRFGRFLGYTE
                     KDLELLYPFKEKVFYNIMRESGYLHIQATKPDTVGCALNDSPVGLAAYILEKFSTWTK
                     SEYRELEDGGLERKFSLEDLLTNIMIYWTTGTIVSSQRFYKENLGQGVMVHRHEGMKV
                     FVPTGYSAFPSEILHAPEKWVKVKYPKLISYSYMERGGHFAAFEEPKLLAQDIRKFVS
                     LAELQ"
     exon            332..512
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            513..740
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            741..870
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            871..1079
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1188
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1314
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1739
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1719..1724
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /note="hexamer: GATAAA"
     polyA_site      1739
                     /gene="Ephx1"
                     /gene_synonym="EH; Eph-1; Eph1; Epxh1; mEH"
                     /note="major polyA site"
ORIGIN      
        1 tgggcggggc tagtgggggc ggggctaaca ggcagctggt gtcaccagct cagcagcagg
       61 caaagaggct caagaggagt ttgagagtgg aggaactgca caccagccgc cgcgggagta
      121 ggaacccgag agcgaccctg acagagtcat gtggctggaa ctcatcctgg cttctgtgct
      181 gggctttgtc atctactggt ttgtctcccg ggacaaggag gagaccttac cacttgaaga
      241 tgggtggtgg ggcccagggt caaagccatc agccaaagaa gatgagagca tccggccctt
      301 caaggtggaa acatcagatg aggagatcaa ggacttgcac cagaggatag ataggttccg
      361 ggcatcccca cctttggagg gcagtcgctt ccactatggc ttcaactcca gctacctgaa
      421 gaaagtggtg tccttctgga ggaatgagtt tgactggagg aagcaggtgg agatcctcaa
      481 ccaataccca cactttaaga ccaagattga agggctggac atccacttca tccacgtgaa
      541 acctccccag ctgccctcag gccgcactcc aaagcccttg ctgatggtgc acggctggcc
      601 tggctccttc tatgagttct acaagattat cccactgctg acagacccca agacccacgg
      661 cctgagtgat gagcacgtgt ttgaagtcat ctgtccctca attcctggct atggcttctc
      721 agaggcatcc agcaagaaag gtttaaattc ggtggccact gcgaggatct tctacaagct
      781 gatgtcacgg ctgggcttcc agaagttcta cattcaaggc ggcgactggg ggtctctcat
      841 ctgcaccaac atagcccaga tggtgcccaa ccacgtgaaa ggtttgcact tgaatatgtc
      901 tttcatttca agaaacattt attccctgac ccctctcctg ggccaacgtt ttgggagatt
      961 tcttggctac acagagaagg atctggagct cttgtaccca ttcaaggaaa aggttttcta
     1021 caacatcatg agggagagtg gctacttaca catccaggcc accaagccgg acactgtggg
     1081 ctgtgctctg aatgactctc ctgtgggcct ggctgcctac atcttagaga agttctccac
     1141 ctggaccaag tcagaatacc gtgaactgga ggatggaggc ctggagagga agttctccct
     1201 ggaagatctg ctgactaaca tcatgatcta ctggacgaca ggaaccattg tctcctccca
     1261 gcgcttctac aaggaaaact tgggccaggg tgtcatggtc catagacatg aggggatgaa
     1321 ggtctttgtg cccactggct attcagcctt cccttctgag atcctgcatg ccccagaaaa
     1381 gtgggtgaag gtcaagtacc ccaaactcat ctcctattcc tacatggaac gtgggggcca
     1441 ctttgctgcc ttcgaagagc ccaagcttct ggcccaggac atccgcaagt tcgtgtccct
     1501 ggctgagctg cagtgatgac gctacacacc aaccatggct ttagcagcag ccctggttcc
     1561 ttcccagtca tacttatgga agatgtgccc ttccagagga ataagtttgt tccctgacca
     1621 cactggggga cccagacttc aaccccacag agtcctctct taccaccccc atatgcttcg
     1681 ccccactgca tagctgtgtt aagctacatg gctttaatga taaatgggtt tatttctaaa
     1741 aaaaaaaaaa aaaa
//
