LOCUS       NM_010144               4334 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus Eph receptor B4 (Ephb4), transcript variant 2, mRNA.
ACCESSION   NM_010144
VERSION     NM_010144.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Trinh,L.T., Finnel,R.R., Osipovich,A.B., Musselman,J.R.,
            Sampson,L.L., Wright,C.V.E. and Magnuson,M.A.
  TITLE     Positive autoregulation of Sox17 is necessary for gallbladder and
            extrahepatic bile duct formation
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39745200
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Hua,R., Mo,Y., Lin,X., Zhang,B., He,M., Huang,C., Huang,Y., Li,J.,
            Wan,J., Qin,H., Xie,Q., Zeng,D. and Sun,Y.
  TITLE     EGR1 modulates EPHB4-induced trophoblast dysfunction in recurrent
            spontaneous abortiondagger
  JOURNAL   Biol Reprod 110 (3), 476-489 (2024)
   PUBMED   38091979
  REMARK    GeneRIF: EGR1 modulates EPHB4-induced trophoblast dysfunction in
            recurrent spontaneous abortiondagger.
REFERENCE   3  (bases 1 to 4334)
  AUTHORS   Lee,J.G., Yon,J.M., Kim,G., Lee,S.G., Kim,C.Y., Cheong,S.A.,
            Kim,H.Y., Yu,J., Kim,K., Sung,Y.H., Yoo,H.J., Woo,D.C., Rho,J.K.,
            Ha,C.H., Pack,C.G., Oh,S.H., Lim,J.S., Han,Y.M., Hong,E.J.,
            Seong,J.K., Lee,H.W., Lee,S.W., Lee,K.U., Kim,C.J., Nam,S.Y.,
            Cho,Y.S. and Baek,I.J.
  TITLE     PIBF1 regulates trophoblast syncytialization and promotes
            cardiovascular development
  JOURNAL   Nat Commun 15 (1), 1487 (2024)
   PUBMED   38374152
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4334)
  AUTHORS   Zhao,S., Mekbib,K.Y., van der Ent,M.A., Allington,G.,
            Prendergast,A., Chau,J.E., Smith,H., Shohfi,J., Ocken,J., Duran,D.,
            Furey,C.G., Hao,L.T., Duy,P.Q., Reeves,B.C., Zhang,J.,
            Nelson-Williams,C., Chen,D., Li,B., Nottoli,T., Bai,S., Rolle,M.,
            Zeng,X., Dong,W., Fu,P.Y., Wang,Y.C., Mane,S., Piwowarczyk,P.,
            Fehnel,K.P., See,A.P., Iskandar,B.J., Aagaard-Kienitz,B.,
            Moyer,Q.J., Dennis,E., Kiziltug,E., Kundishora,A.J., DeSpenza,T.
            Jr., Greenberg,A.B.W., Kidanemariam,S.M., Hale,A.T., Johnston,J.M.,
            Jackson,E.M., Storm,P.B., Lang,S.S., Butler,W.E., Carter,B.S.,
            Chapman,P., Stapleton,C.J., Patel,A.B., Rodesch,G., Smajda,S.,
            Berenstein,A., Barak,T., Erson-Omay,E.Z., Zhao,H.,
            Moreno-De-Luca,A., Proctor,M.R., Smith,E.R., Orbach,D.B.,
            Alper,S.L., Nicoli,S., Boggon,T.J., Lifton,R.P., Gunel,M.,
            King,P.D., Jin,S.C. and Kahle,K.T.
  TITLE     Mutation of key signaling regulators of cerebrovascular development
            in vein of Galen malformations
  JOURNAL   Nat Commun 14 (1), 7452 (2023)
   PUBMED   37978175
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4334)
  AUTHORS   Cao,X., Li,T., Xu,B., Ding,K., Li,W., Shen,B., Chu,M., Zhu,D.,
            Rui,L., Shang,Z., Li,X., Wang,Y., Zheng,S., Alitalo,K., Liu,G.,
            Tang,J., Kubota,Y. and He,Y.
  TITLE     Endothelial TIE1 Restricts Angiogenic Sprouting to Coordinate Vein
            Assembly in Synergy With Its Homologue TIE2
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (8), e323-e338 (2023)
   PUBMED   37317851
REFERENCE   6  (bases 1 to 4334)
  AUTHORS   .
  TITLE     Unified nomenclature for Eph family receptors and their ligands,
            the ephrins. Eph Nomenclature Committee
  JOURNAL   Cell 90 (3), 403-404 (1997)
   PUBMED   9267020
REFERENCE   7  (bases 1 to 4334)
  AUTHORS   Berclaz,G., Andres,A.C., Albrecht,D., Dreher,E., Ziemiecki,A.,
            Gusterson,B.A. and Crompton,M.R.
  TITLE     Expression of the receptor protein tyrosine kinase myk-1/htk in
            normal and malignant mammary epithelium
  JOURNAL   Biochem Biophys Res Commun 226 (3), 869-875 (1996)
   PUBMED   8831703
REFERENCE   8  (bases 1 to 4334)
  AUTHORS   Ciossek,T., Lerch,M.M. and Ullrich,A.
  TITLE     Cloning, characterization, and differential expression of MDK2 and
            MDK5, two novel receptor tyrosine kinases of the eck/eph family
  JOURNAL   Oncogene 11 (10), 2085-2095 (1995)
   PUBMED   7478528
REFERENCE   9  (bases 1 to 4334)
  AUTHORS   Hebert,B., Bergeron,J., Tijssen,P. and Potworowski,E.F.
  TITLE     Protein tyrosine kinases transcribed in a murine thymic medullary
            epithelial cell line
  JOURNAL   Gene 143 (2), 257-260 (1994)
   PUBMED   8206383
REFERENCE   10 (bases 1 to 4334)
  AUTHORS   Andres,A.C., Reid,H.H., Zurcher,G., Blaschke,R.J., Albrecht,D. and
            Ziemiecki,A.
  TITLE     Expression of two novel eph-related receptor protein tyrosine
            kinases in mammary gland development and carcinogenesis
  JOURNAL   Oncogene 9 (5), 1461-1467 (1994)
   PUBMED   8152808
  REMARK    Erratum:[Oncogene 1994 Aug;9(8):2431]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK132392.1, BC090839.1, AK049340.1 and BB677774.1.
            
            On Apr 22, 2009 this sequence version replaced NM_010144.5.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. This
            results in a shorter protein (isoform b) compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132392.1, AK049340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1588              AK132392.1         1-1588
            1589-2703           BC090839.1         1366-2480
            2704-4322           AK049340.1         2705-4323
            4323-4334           BB677774.1         425-436
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 76.38 cM"
     gene            1..4334
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /note="Eph receptor B4"
                     /db_xref="GeneID:13846"
                     /db_xref="MGI:MGI:104757"
     exon            1..541
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     CDS             490..3453
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /EC_number="2.7.10.1"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; ephrin type-B receptor 4; mDK-2; tyrosine
                     kinase MYK-1; developmental kinase 2; hepatoma
                     transmembrane kinase"
                     /codon_start=1
                     /product="ephrin type-B receptor 4 isoform b precursor"
                     /protein_id="NP_034274.4"
                     /db_xref="CCDS:CCDS19767.1"
                     /db_xref="GeneID:13846"
                     /db_xref="MGI:MGI:104757"
                     /translation="MELRALLCWASLATALEETLLNTKLETADLKWVTYPQAEGQWEE
                     LSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPR
                     ASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVN
                     IKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPETVPRELVVP
                     VAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTF
                     KPQIGDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHL
                     NGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVA
                     IRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDREVPPAVSDIRVTRSSPSSL
                     ILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVR
                     ARSEAGYGPFGQEHHSQTQLDESESWREQLALIAGTAVVGVVLVLVVVIIAVLCLRKQ
                     SNGREVEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAG
                     EFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGV
                     VTNSVPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRD
                     LAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFT
                     SASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDC
                     WQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGE
                     WLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQA
                     KPGAPGGTGGPAQQF"
     exon            542..612
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            613..900
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1297
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1453
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1786
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1911
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2077
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2078..2180
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2245
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2359
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2607
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2823
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2973
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            2974..3167
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            3168..3323
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..4334
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      3651
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /note="major polyA site"
     regulatory      4076..4081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /note="hexamer: AACAAG"
     polyA_site      4130
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
     regulatory      4272..4277
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /note="hexamer: ATTATA"
     polyA_site      4289
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /note="major polyA site"
     regulatory      4306..4311
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
                     /note="hexamer: AATAAA"
     polyA_site      4326
                     /gene="Ephb4"
                     /gene_synonym="b2b2412Clo; Htk; MDK2; Myk1; Tyro11"
ORIGIN      
        1 agtctcgact cggcccagcc ctggcccagc ctgtttccct ccctcctgct cggttcccac
       61 tttggtttcc accttccttg accgcgaccg gcgcactaac cccggcggcg cgacatccac
      121 caaccactcc agggagcgag agagaccgcg agcaatcggc ccaccccagc cagccagggc
      181 gggacccccg aagccccgaa gggaccccta actctcagcc acgtcccgat ctccgcccgc
      241 ccagcgacgg ccactgccag gacgtccccc ctcaaggctg gcctccccgc gcgcgcgcgc
      301 tgcggcgggg acgtgggggc cgcacctgag cgcgggtgcc tggcgcgccc gcatgggcca
      361 tcgttgagag gccctcgacg gaaagtccca gcagcagagt cggtgtgcac ccgcccaggg
      421 gagggtcaga cctggggggg ccagggcccc ccaaactcgg atcgcattca gccaaagtga
      481 ggcggcgcca tggagctccg agcgctgctg tgctgggctt ccctcgccac tgctttagaa
      541 gagaccctgt tgaacacaaa actggaaacg gcggatctga aatgggtgac ttaccctcag
      601 gcagagggcc agtgggagga gctaagcggc ctggatgagg aacagcacag cgtccgcacc
      661 tatgaggtgt gcgacatgaa gcgtccaggg ggccaggctc actggctgcg cactggctgg
      721 gtcccaaggc gaggtgctgt ccacgtgtat gccacgatac gcttcaccat gatggaatgc
      781 ctgtccctgc cgagggccag tcgctcctgc aaggagacat tcactgtctt ctattacgag
      841 agcgaagctg atacggccac ggcccatacg cccgcctgga tggagaaccc ctacatcaag
      901 gtggacacag tggccgcaga acatctgact cggaagcgcc ctggagctga agccacaggg
      961 aaagttaata tcaagacgct gcgcctgggt cctctcagca aagctggctt ctacctggct
     1021 ttccaggacc aaggagcctg catggctctg ctctccctgc atctctttta caagaagtgc
     1081 tcctggctga tcacgaactt gacctacttc cccgagacgg tgcctcggga gctcgtggtg
     1141 ccggtggcag gtagctgcgt ggccaacgcg gtccctaccg ccaaccccag ccccagcctc
     1201 tactgccggg aagatggtca atgggctgag cagcaggtca cgggctgcag ctgcgcgcca
     1261 gggtacgagg ctgcggaaag caacaaagta tgcagagcct gtggccaggg aaccttcaag
     1321 ccccaaatag gagacgagtc ctgcctgcca tgcccagcca acagccactc gaataacatt
     1381 gggtctcctg tctgcctgtg tcgaattggg tattaccggg cccgctcaga cccccggagt
     1441 tcaccttgca ctaccccacc ctctgctcca agaagcgtgg ttcaccattt gaatggttcc
     1501 accctgcgcc tggaatggag tgctcccctt gagtccggag gccgagagga cctcacttat
     1561 gctgtacgct gccgagagtg ccgtcctggg ggttcctgct tgccctgtgg gggcgacatg
     1621 accttcgacc ccggtcctcg agacctggtt gagccctggg tggcaatccg agggctgcgt
     1681 cctgatgtca cctatacctt tgaggttgct gctttgaatg gtgtgtctac cttagccact
     1741 ggaccacctc cttttgagcc tgtcaatgtc accactgacc gtgaggtgcc tcctgcagtg
     1801 tctgacatcc gagtgactcg gtcgtcaccc agcagcttga tcctgtcatg ggctatcccc
     1861 agagcaccca gtggggccgt gctggactac gaggtcaagt atcatgagaa gggcgcagag
     1921 ggccccagca gtgttcgttt cctgaagaca tcagaaaacc gagctgagct ccgggggctg
     1981 aagcggggag ccagctatct ggtccaggta cgcgcacggt ccgaggctgg ctacggtccc
     2041 ttcggccagg agcatcacag tcagactcaa ctggatgaga gcgagagctg gcgggagcag
     2101 ctggccctga ttgcaggcac tgcggttgtg ggtgtggtcc tggtcctggt ggtcgtcatc
     2161 attgcagttc tctgcctcag gaagcagagc aatgggaggg aagttgagta ctcggataag
     2221 catgggcagt atctcatcgg gcacggtacc aaggtctaca ttgatccttt tacttacgaa
     2281 gaccctaatg aggcagtgag ggaatttgcc aaagagatcg atgtctccta tgtcaagatt
     2341 gaagaggtaa ttggtgcagg tgagttcggc gaggtgtgcc ggggtcggct gaaggcacca
     2401 gggaaaaagg agagctgtgt ggccatcaag actctgaagg gtggctacac cgagcgccag
     2461 aggcgtgagt tcctgagcga ggcctccatc atgggccagt tcgagcatcc caacatcatc
     2521 cgcctcgagg gcgtggtcac caacagtgtg ccggttatga tcctcacgga attcatggag
     2581 aacggagccc tggactcctt cctgcggctg aacgacgggc agttcacagt catccagctg
     2641 gtgggcatgc tgaggggcat cgcctcgggc atgcggtacc tggctgaaat gagctatgtc
     2701 caccgagacc tggctgctcg gaacatcttg gtcaacagta acctggtctg caaggtgtcc
     2761 gactttggcc tctccagatt cttggaggag aactcctctg atcccaccta cacaagttcc
     2821 ctgggaggca agattcccat ccgatggacc gcccctgaag ccattgcctt caggaagttc
     2881 acctctgcca gtgatgcctg gagctatggg atcgtcatgt gggaggtcat gtcttttggg
     2941 gaacggccat actgggacat gagcaaccag gatgtgatca atgccattga acaggactac
     3001 cggctgcctc ctcctccaga ctgccccacc tccctccacc agctcatgct ggattgttgg
     3061 cagaaggacc ggaatgcccg gccccgcttt ccccaggtgg tcagcgctct ggacaagatg
     3121 atccggaatc ccgctagcct caaaatcgtg gccagggaga atggcggggc ctcacatcca
     3181 ctcttggacc aacggcagcc tcactattct gctttcggtt ctgtgggcga gtggcttcga
     3241 gccatcaaga tgggaagata cgaggaaagt tttgcagcgg ctggattcgg ctcctttgag
     3301 gtggtcagtc agatctctgc cgaggacctt ctccgaattg gagtcactct ggcaggacac
     3361 cagaagaaaa tcttggccag tgtgcagcat atgaagtccc aagctaagcc aggagcccct
     3421 ggtgggacag ggggaccagc ccagcagttc tgacctccaa ggactcacca ccgtggcaga
     3481 ttcttctttc cggggggcag agttgggtgg ggactcacaa gatgagcccc tccccctcgt
     3541 cacagccttc ccattggatt gcactttgaa cagagggggt cagagaccca ggatttgggg
     3601 aaccgtgcca tatgggatca tacatgtgcc ctccaggcgg ggaaccccaa actcagagtg
     3661 agtctttccc tcaagactgg gcaaagaaac atccctacgt ctctaacctc ccatcttccc
     3721 agagggctct ctccccaagc gccttccacc tcaacgggca tgtccctgca gaccaaagag
     3781 aaagggtgac cagcctgcca acttgggagt ggaaaatgcc gtcccaggag gcaggaaggg
     3841 gctgtcagga cccggtgatg taatcattgg gttttgatgt cctgacttgc tgtcaccacc
     3901 aaaggcaatc atttttccct tgtaaatgcc cctcccctca tctgccttca tattgaaggt
     3961 tctgaagttt tactgttttt tattttgtta attttttcct ccttcccccc tccctcccct
     4021 tcttgtccag attttgtgtg ttaaagggca cctggttcca ctatctcctg ttgggaacaa
     4081 ggacccatcg atatgttcta gaacagtgcc ttggaaatgc cacagcctta gacagttcct
     4141 tcttcctttc ctccccccaa gtgcccccaa gctgtgttgt gtggagggaa ggggggtgat
     4201 gttgtaaaag ggacaggagg ttggtggtgg tcaacagagg cagacaacgg tgcttggaag
     4261 ggggttctta aattatattt aaaaagaaac tttttttttt ttataaataa aaaaaaaagg
     4321 gggatatgtc tcag
//
