LOCUS       NP_034272                986 aa            linear   ROD 12-NOV-2024
DEFINITION  ephrin type-B receptor 2 isoform 2 precursor [Mus musculus].
ACCESSION   NP_034272 XP_204072
VERSION     NP_034272.1
DBSOURCE    REFSEQ: accession NM_010142.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 986)
  AUTHORS   David,E.T., Yousuf,M.S., Mei,H.R., Jain,A., Krishnagiri,S.,
            Elahi,H., Venkatesan,R., Srikanth,K.D., Dussor,G., Dalva,M.B. and
            Price,T.J.
  TITLE     ephrin-B2 promotes nociceptive plasticity and hyperalgesic priming
            through EphB2-MNK-eIF4E signaling in both mice and humans
  JOURNAL   Pharmacol Res 206, 107284 (2024)
   PUBMED   38925462
  REMARK    GeneRIF: ephrin-B2 promotes nociceptive plasticity and hyperalgesic
            priming through EphB2-MNK-eIF4E signaling in both mice and humans.
REFERENCE   2  (residues 1 to 986)
  AUTHORS   Agarwal,K. and Lamprecht,R.
  TITLE     EphB2 activation in neural stem cells in the basolateral amygdala
            facilitates neurogenesis and enhances long-term memory
  JOURNAL   Cell Mol Life Sci 81 (1), 277 (2024)
   PUBMED   38913115
  REMARK    GeneRIF: EphB2 activation in neural stem cells in the basolateral
            amygdala facilitates neurogenesis and enhances long-term memory.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 986)
  AUTHORS   Zhang,H., Lei,M., Zhang,Y., Li,H., He,Z., Xie,S., Zhu,L., Wang,S.,
            Liu,J., Li,Y., Lu,Y. and Ma,C.
  TITLE     Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE
            complex assembly and neurotransmitter release
  JOURNAL   Sci Adv 10 (20), eadi7024 (2024)
   PUBMED   38758791
  REMARK    GeneRIF: Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated
            SNARE complex assembly and neurotransmitter release.
REFERENCE   4  (residues 1 to 986)
  AUTHORS   Montero-Herradon,S., Garcia-Ceca,J., Villarejo-Torres,M. and
            Zapata,A.G.
  TITLE     Peripheral T-cell responses of EphB2- and EphB3-deficient mice in a
            model of collagen-induced arthritis
  JOURNAL   Cell Mol Life Sci 81 (1), 159 (2024)
   PUBMED   38558087
  REMARK    GeneRIF: Peripheral T-cell responses of EphB2- and EphB3-deficient
            mice in a model of collagen-induced arthritis.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 986)
  AUTHORS   Rasool,D., Burban,A., Sharanek,A., Madrigal,A., Hu,J., Yan,K.,
            Qu,D., Voss,A.K., Slack,R.S., Thomas,T., Bonni,A., Picketts,D.J.,
            Soleimani,V.D., Najafabadi,H.S. and Jahani-Asl,A.
  TITLE     PHF6-mediated transcriptional control of NSC via Ephrin receptors
            is impaired in the intellectual disability syndrome BFLS
  JOURNAL   EMBO Rep 25 (3), 1256-1281 (2024)
   PUBMED   38429579
REFERENCE   6  (residues 1 to 986)
  AUTHORS   Singh,A., Winterbottom,E. and Daar,I.O.
  TITLE     Eph/ephrin signaling in cell-cell and cell-substrate adhesion
  JOURNAL   Front Biosci (Landmark Ed) 17 (2), 473-497 (2012)
   PUBMED   22201756
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (residues 1 to 986)
  AUTHORS   Bergemann,A.D., Cheng,H.J., Brambilla,R., Klein,R. and
            Flanagan,J.G.
  TITLE     ELF-2, a new member of the Eph ligand family, is segmentally
            expressed in mouse embryos in the region of the hindbrain and newly
            forming somites
  JOURNAL   Mol Cell Biol 15 (9), 4921-4929 (1995)
   PUBMED   7651410
REFERENCE   8  (residues 1 to 986)
  AUTHORS   Sommers,C.L., Huang,K., Shores,E.W., Grinberg,A., Charlick,D.A.,
            Kozak,C.A. and Love,P.E.
  TITLE     Murine txk: a protein tyrosine kinase gene regulated by T cell
            activation
  JOURNAL   Oncogene 11 (2), 245-251 (1995)
   PUBMED   7542761
REFERENCE   9  (residues 1 to 986)
  AUTHORS   Saito,T., Seki,N., Matsuda,Y., Kitahara,M., Murata,M., Kanda,N.,
            Nomura,N., Yamamoto,T. and Hori,T.A.
  TITLE     Identification of the human ERK gene as a putative receptor
            tyrosine kinase and its chromosomal localization to 1p36.1: a
            comparative mapping of human, mouse, and rat chromosomes
  JOURNAL   Genomics 26 (2), 382-384 (1995)
   PUBMED   7601466
REFERENCE   10 (residues 1 to 986)
  AUTHORS   Ben-David,Y., Letwin,K., Tannock,L., Bernstein,A. and Pawson,T.
  TITLE     A mammalian protein kinase with potential for serine/threonine and
            tyrosine phosphorylation is related to cell cycle regulators
  JOURNAL   EMBO J 10 (2), 317-325 (1991)
   PUBMED   1825055
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ180821.1, BC043088.1 and
            AL671173.12.
            
            On Aug 30, 2004 this sequence version replaced XP_204072.3.
            
            Summary: This gene encodes a member of the Eph receptor family of
            receptor tyrosine kinase transmembrane glycoproteins. These
            receptors consist of an N-terminal glycosylated ligand-binding
            domain, a transmembrane region and an intracellular kinase domain.
            The encoded receptor preferentially binds membrane-bound ephrin-B
            ligands and is involved in nervous system and vascular development.
            This gene is used as a marker of intestinal stem cells. Homozygous
            knockout mice for this gene exhibit impaired axon guidance and
            vestibular function. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. It
            encodes isoform 2, which is shorter by an amino acid, compared to
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043088.1, SRR7974084.22998.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Mus musculus"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 69.0 cM"
     Protein         1..986
                     /product="ephrin type-B receptor 2 isoform 2 precursor"
                     /EC_number="2.7.10.1"
                     /note="ephrin type-B receptor 2; neural kinase; nuk
                     receptor tyrosine kinase; tyrosine-protein kinase receptor
                     EPH-3; tyrosine-protein kinase receptor SEK-3"
                     /calculated_mol_wt=107353
     sig_peptide     1..24
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2565
     Region          19..196
                     /region_name="EphR_LBD_B2"
                     /note="Ligand Binding Domain of Ephrin type-B Receptor 2;
                     cd10477"
                     /db_xref="CDD:198445"
     Site            order(28..30,32,44..47,49,53,55,57,60,93..95,99,101,147,
                     149..156,158..159,184,186,188)
                     /site_type="other"
                     /note="ephrin binding site [polypeptide binding]"
                     /db_xref="CDD:198445"
     Site            order(325,400,415)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          326..421
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Site            order(416,418,420..421)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Site            order(436,500,515)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          437..520
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Site            order(516..517,519..520)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          544..617
                     /region_name="EphA2_TM"
                     /note="Ephrin type-A receptor 2 transmembrane domain;
                     pfam14575"
                     /db_xref="CDD:464211"
     Region          616..884
                     /region_name="PTKc_EphR_B"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Class EphB Ephrin Receptors; cd05065"
                     /db_xref="CDD:173638"
     Site            order(627..631,635,651,653,699..700,702,706,746,750..751,
                     753,764,785..789,798,832)
                     /site_type="active"
                     /db_xref="CDD:173638"
     Site            order(627..629,631,635,651,653,699..700,702,706,751,753,
                     764)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:173638"
     Site            order(746,750,785..789,798,832)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:173638"
     Site            763..791
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:173638"
     Region          910..980
                     /region_name="SAM_EPH-B2"
                     /note="SAM domain of EPH-B2 subfamily of tyrosine kinase
                     receptors; cd09552"
                     /db_xref="CDD:188951"
     Site            order(912..913,915,937,943..944,952,960..963,965..966,974,
                     978)
                     /site_type="other"
                     /note="homooligomer interface [polypeptide binding]"
                     /db_xref="CDD:188951"
     Site            order(912,915,921,942,970,973..974)
                     /site_type="other"
                     /note="putative homodimer interface (head-to-head)
                     [polypeptide binding]"
                     /db_xref="CDD:188951"
     Site            order(930,963)
                     /site_type="other"
                     /note="putative phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:188951"
     Site            order(946..951,954,972,975,978..979)
                     /site_type="other"
                     /note="homodimer interface (tail-to-tail) [polypeptide
                     binding]"
                     /db_xref="CDD:188951"
     CDS             1..986
                     /gene="Ephb2"
                     /gene_synonym="Cek5; Drt; Erk; ETECK; Hek5; Nuk; Prkm5;
                     Qek5; Sek3; Tyro5"
                     /coded_by="NM_010142.4:183..3143"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /db_xref="CCDS:CCDS18809.1"
                     /db_xref="GeneID:13844"
                     /db_xref="MGI:MGI:99611"
ORIGIN      
        1 mavrrlgaal lllpllaave etlmdsttat aelgwmvhpp sgweevsgyd enmntirtyq
       61 vcnvfessqn nwlrtkfirr rgahrihvem kfsvrdcssi psvpgscket fnlyyyeadf
      121 dlatktfpnw menpwvkvdt iaadesfsqv dlggrvmkin tevrsfgpvs rngfylafqd
      181 yggcmsliav rvfyrkcpri iqngaifqet lsgaestslv aargsciana eevdvpikly
      241 cngdgewlvp igrcmckagf eavengtvcr gcpsgtfkan qgdeacthcp insrttsega
      301 tncvcrngyy radldpldmp cttipsapqa vissvnetsl mlewtpprds ggredlvyni
      361 ickscgsgrg actrcgdnvq yaprqlglte priyisdlla htqytfeiqa vngvtdqspf
      421 spqfasvnit tnqaapsavs imhqvsrtvd sitlswsqpd qpngvildye lqyyekelse
      481 ynataikspt ntvtvqglka gaiyvfqvra rtvagygrys gkmyfqtmte aeyqtsikek
      541 lplivgssaa glvfliavvv iaivcnrrgf eradseytdk lqhytsghmt pgmkiyidpf
      601 tyedpneavr efakeidisc vkieqvigag efgevcsghl klpgkreifv aiktlksgyt
      661 ekqrrdflse asimgqfdhp nvihlegvvt kstpvmiite fmengsldsf lrqndgqftv
      721 iqlvgmlrgi aagmkyladm nyvhrdlaar nilvnsnlvc kvsdfglsrf leddtsdpty
      781 tsalggkipi rwtapeaiqy rkftsasdvw sygivmwevm sygerpywdm tnqdvinaie
      841 qdyrlpppmd cpsalhqlml dcwqkdrnhr pkfgqivntl dkmirnpnsl kamaplssgi
      901 nlplldrtip dytsfntvde wleaikmgqy kesfanagft sfdvvsqmmm edilrvgvtl
      961 aghqkkilns iqvmraqmnq iqsvev
//
