LOCUS       NP_034270                984 aa            linear   ROD 27-APR-2025
DEFINITION  ephrin type-A receptor 3 isoform 1 precursor [Mus musculus].
ACCESSION   NP_034270
VERSION     NP_034270.1
DBSOURCE    REFSEQ: accession NM_010140.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 984)
  AUTHORS   Lo Giudice,Q., Wagener,R.J., Abe,P., Frangeul,L. and Jabaudon,D.
  TITLE     Developmental emergence of first- and higher-order thalamic neuron
            molecular identities
  JOURNAL   Development 151 (18) (2024)
   PUBMED   39348458
REFERENCE   2  (residues 1 to 984)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 984)
  AUTHORS   Alderman,P.J., Saxon,D., Torrijos-Saiz,L.I., Sharief,M., Page,C.E.,
            Baroudi,J.K., Biagiotti,S.W., Butyrkin,V.A., Melamed,A., Kuo,C.T.,
            Vicini,S., Garcia-Verdugo,J.M., Herranz-Perez,V., Corbin,J.G. and
            Sorrells,S.F.
  TITLE     Delayed maturation and migration of excitatory neurons in the
            juvenile mouse paralaminar amygdala
  JOURNAL   Neuron 112 (4), 574-592 (2024)
   PUBMED   38086370
REFERENCE   4  (residues 1 to 984)
  AUTHORS   Lee,Y., Yeo,I.S., Kim,N., Lee,D.K., Kim,K.T., Yoon,J., Yi,J.,
            Hong,Y.B., Choi,B.O., Kosodo,Y., Kim,D., Park,J. and Song,M.R.
  TITLE     Transcriptional control of motor pool formation and motor circuit
            connectivity by the LIM-HD protein Isl2
  JOURNAL   Elife 12, e84596 (2023)
   PUBMED   37869988
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 984)
  AUTHORS   Ozekin,Y.H., O'Rourke,R. and Bates,E.A.
  TITLE     Single cell sequencing of the mouse anterior palate reveals
            mesenchymal heterogeneity
  JOURNAL   Dev Dyn 252 (6), 713-727 (2023)
   PUBMED   36734036
REFERENCE   6  (residues 1 to 984)
  AUTHORS   Cheng,H.J. and Flanagan,J.G.
  TITLE     Identification and cloning of ELF-1, a developmentally expressed
            ligand for the Mek4 and Sek receptor tyrosine kinases
  JOURNAL   Cell 79 (1), 157-168 (1994)
   PUBMED   7522971
REFERENCE   7  (residues 1 to 984)
  AUTHORS   Fujimoto,J. and Yamamoto,T.
  TITLE     brt, a mouse gene encoding a novel receptor-type protein-tyrosine
            kinase, is preferentially expressed in the brain
  JOURNAL   Oncogene 9 (3), 693-698 (1994)
   PUBMED   8108111
REFERENCE   8  (residues 1 to 984)
  AUTHORS   Gilardi-Hebenstreit,P., Nieto,M.A., Frain,M., Mattei,M.G.,
            Chestier,A., Wilkinson,D.G. and Charnay,P.
  TITLE     An Eph-related receptor protein tyrosine kinase gene segmentally
            expressed in the developing mouse hindbrain
  JOURNAL   Oncogene 7 (12), 2499-2506 (1992)
   PUBMED   1281307
  REMARK    Erratum:[Oncogene. 1993 Apr;8(4):1103. PMID: 8455939]
REFERENCE   9  (residues 1 to 984)
  AUTHORS   Sajjadi,F.G., Pasquale,E.B. and Subramani,S.
  TITLE     Identification of a new eph-related receptor tyrosine kinase gene
            from mouse and chicken that is developmentally regulated and
            encodes at least two forms of the receptor
  JOURNAL   New Biol 3 (8), 769-778 (1991)
   PUBMED   1657122
REFERENCE   10 (residues 1 to 984)
  AUTHORS   Lai,C. and Lemke,G.
  TITLE     An extended family of protein-tyrosine kinase genes differentially
            expressed in the vertebrate nervous system
  JOURNAL   Neuron 6 (5), 691-704 (1991)
   PUBMED   2025425
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154844.2, AC155275.1 and AC154568.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC093483.1, AK132335.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 36.49 cM"
     Protein         1..984
                     /product="ephrin type-A receptor 3 isoform 1 precursor"
                     /EC_number="2.7.10.1"
                     /note="ephrin type-A receptor 3; EPH-like kinase 4;
                     tyrosine-protein kinase TYRO4; tyrosine-protein kinase
                     receptor ETK1"
                     /calculated_mol_wt=107990
     sig_peptide     1..20
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2067
     Region          29..201
                     /region_name="EphR_LBD_A3"
                     /note="Ligand Binding Domain of Ephrin type-A Receptor 3;
                     cd10481"
                     /db_xref="CDD:198449"
     Site            order(53,55..59,66..67,69,71,102,104,109..110,152,
                     156..157,160..162,189..191,193)
                     /site_type="other"
                     /note="ephrin binding site [polypeptide binding]"
                     /db_xref="CDD:198449"
     Site            232
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Region          <323..>516
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            337
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Site            391
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Site            404
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Region          437..528
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(437,501,516)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            493
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Site            order(517..518,520..521)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Site            542..565
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P29319.1)"
     Region          <566..619
                     /region_name="EphA2_TM"
                     /note="Ephrin type-A receptor 2 transmembrane domain;
                     pfam14575"
                     /db_xref="CDD:464211"
     Site            597
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P29320; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Site            603
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P29320; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Region          617..883
                     /region_name="PTKc_EphR_A"
                     /note="Catalytic domain of the Protein Tyrosine Kinases,
                     Class EphA Ephrin Receptors; cd05066"
                     /db_xref="CDD:270651"
     Site            order(628,630..632,636,652,654,700..701,703,747,751..752,
                     754,765,784..788,797,831)
                     /site_type="active"
                     /db_xref="CDD:270651"
     Site            order(628..632,636,652,654,700..703,706..707,751..752,754,
                     765)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270651"
     Site            702
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P29320; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Site            order(747,751,784..788,797,831)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270651"
     Site            764..790
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270651"
     Site            780
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000250|UniProtKB:P29320; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Region          913..975
                     /region_name="SAM_EPH-A3"
                     /note="SAM domain of EPH-A3 subfamily of tyrosine kinase
                     receptors; cd09544"
                     /db_xref="CDD:188943"
     Site            938
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:17947660; propagated from
                     UniProtKB/Swiss-Prot (P29319.1)"
     Region          982..984
                     /region_name="PDZ-binding. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (P29319.1)"
     CDS             1..984
                     /gene="Epha3"
                     /gene_synonym="Cek4; EK4; End3; ETK1; Hek; Hek4; Mek4;
                     Tyro4"
                     /coded_by="NM_010140.4:129..3083"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS28264.1"
                     /db_xref="GeneID:13837"
                     /db_xref="MGI:MGI:99612"
ORIGIN      
        1 mdchlsilvl lgccvlscsg elspqpsnev nlldsktiqg elgwisypsh gweeisgvde
       61 hytpirtyqv cnvmdhsqnn wlrtnwvprn saqkiyvelk ftlrdcnsip lvlgtcketf
      121 nlyymesddd hgvkfrehqf tkidtiaade sftqmdlgdr ilklnteire vgpvnkkgfy
      181 lafqdvgacv alvsvrvyfk kcpftvknla mfpdtvpmds qslvevrgsc vnnskeedpp
      241 rmycstegew lvpigkctcn agyeergfic qacrpgfyka sdgaakcakc pphsstqedg
      301 smncrcenny fraekdppsm actrppsapr nvisninets vildwswpld tggrkditfn
      361 iickkcgwnv rqcepcspnv rflprqlglt nttvtvtdll ahtnytfeid avngvselss
      421 pprqyaavsi ttnqaapspv mtikkdrtsr nsislswqep ehpngiildy evkyyekqeq
      481 etsytilrar gtnvtisslk pdttyvfqir artaagygtn srkfefetsp dsfsisgens
      541 hvvmiaisaa vaiivltvvt yvlvgrfcgy hkskhsaeek rlhfgnghlk lpglrtyvdp
      601 htyedptqav hefakeldat nisidkvvga gefgevcsgr lklpskkeis vaiktlkvgy
      661 tekqrrdflg easimgqfdh pniirlegvv tkskpvmivt eymengslds flrkhdaqft
      721 viqlvgmlrg iasgmkylsd mgyvhrdlaa rnilinsnlv ckvsdfglsr vleddpeaay
      781 ttrggkipir wtspeaiayr kftsasdvws ygivlwevms ygerpywems nqdvikavde
      841 gyrlpppmdc paalyqlmld cwqkdrnnrp kfeqivsild klirnpgslk iitsaaarps
      901 nllldqsnvd iatfhttgdw lngmrtahck eiftgveyss cdtiakistd dmkkvgvtvv
      961 gpqkkiisti kaletqskng pvpv
//
