LOCUS       NM_053087               1704 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus epithelial mitogen (Epgn), mRNA.
ACCESSION   NM_053087 XM_357479
VERSION     NM_053087.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 1704)
  AUTHORS   Dahlhoff,M., Frances,D., Kloepper,J.E., Paus,R., Schafer,M.,
            Niemann,C. and Schneider,M.R.
  TITLE     Overexpression of epigen during embryonic development induces
            reversible, epidermal growth factor receptor-dependent sebaceous
            gland hyperplasia
  JOURNAL   Mol Cell Biol 34 (16), 3086-3095 (2014)
   PUBMED   24891618
  REMARK    GeneRIF: activation of the EGFR by its ligand epigen during early
            epidermal development causes hyperplasia of the sebaceous gland
            and, to a minor extent, hyperplasia of the interfollicular
            epidermis and increased hair follicles density.
REFERENCE   4  (bases 1 to 1704)
  AUTHORS   Hosur,V., Johnson,K.R., Burzenski,L.M., Stearns,T.M., Maser,R.S.
            and Shultz,L.D.
  TITLE     Rhbdf2 mutations increase its protein stability and drive EGFR
            hyperactivation through enhanced secretion of amphiregulin
  JOURNAL   Proc Natl Acad Sci U S A 111 (21), E2200-E2209 (2014)
   PUBMED   24825892
REFERENCE   5  (bases 1 to 1704)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1704)
  AUTHORS   Sahin,U. and Blobel,C.P.
  TITLE     Ectodomain shedding of the EGF-receptor ligand epigen is mediated
            by ADAM17
  JOURNAL   FEBS Lett 581 (1), 41-44 (2007)
   PUBMED   17169360
REFERENCE   7  (bases 1 to 1704)
  AUTHORS   Kobielak,A. and Fuchs,E.
  TITLE     Links between alpha-catenin, NF-kappaB, and squamous cell carcinoma
            in skin
  JOURNAL   Proc Natl Acad Sci U S A 103 (7), 2322-2327 (2006)
   PUBMED   16452166
REFERENCE   8  (bases 1 to 1704)
  AUTHORS   Kochupurakkal,B.S., Harari,D., Di-Segni,A., Maik-Rachline,G.,
            Lyass,L., Gur,G., Kerber,G., Citri,A., Lavi,S., Eilam,R.,
            Chalifa-Caspi,V., Eshhar,Z., Pikarsky,E., Pinkas-Kramarski,R.,
            Bacus,S.S. and Yarden,Y.
  TITLE     Epigen, the last ligand of ErbB receptors, reveals intricate
            relationships between affinity and mitogenicity
  JOURNAL   J Biol Chem 280 (9), 8503-8512 (2005)
   PUBMED   15611079
  REMARK    GeneRIF: EPG is an EGF-like growth factor and a low affinity ligand
REFERENCE   9  (bases 1 to 1704)
  AUTHORS   Johnson,K.R., Lane,P.W., Cook,S.A., Harris,B.S., Ward-Bailey,P.F.,
            Bronson,R.T., Lyons,B.L., Shultz,L.D. and Davisson,M.T.
  TITLE     Curly bare (cub), a new mouse mutation on chromosome 11 causing
            skin and hair abnormalities, and a modifier gene (mcub) on
            chromosome 5
  JOURNAL   Genomics 81 (1), 6-14 (2003)
   PUBMED   12573256
REFERENCE   10 (bases 1 to 1704)
  AUTHORS   Strachan,L., Murison,J.G., Prestidge,R.L., Sleeman,M.A.,
            Watson,J.D. and Kumble,K.D.
  TITLE     Cloning and biological activity of epigen, a novel member of the
            epidermal growth factor superfamily
  JOURNAL   J Biol Chem 276 (21), 18265-18271 (2001)
   PUBMED   11278323
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC109311.12.
            
            On Nov 17, 2006 this sequence version replaced NM_053087.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ291391.1, BC119293.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AC109311.12        119059-119107       c
            50-136              AC109311.12        118238-118324       c
            137-257             AC109311.12        115396-115516       c
            258-410             AC109311.12        114265-114417       c
            411-1704            AC109311.12        111412-112705       c
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 44.78 cM"
     gene            1..1704
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /note="epithelial mitogen"
                     /db_xref="GeneID:71920"
                     /db_xref="MGI:MGI:1919170"
     exon            1..49
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /inference="alignment:Splign:2.1.0"
     CDS             7..465
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /note="EPG"
                     /codon_start=1
                     /product="epigen precursor"
                     /protein_id="NP_444317.1"
                     /db_xref="CCDS:CCDS19419.1"
                     /db_xref="GeneID:71920"
                     /db_xref="MGI:MGI:1919170"
                     /translation="MALGVLIAVCLLFKAMKAALSEEAEVIPPSTAQQSNWTFNNTEA
                     DYIEEPVALKFSHPCLEDHNSYCINGACAFHHELKQAICRCFTGYTGQRCEHLTLTSY
                     AVDSYEKYIAIGIGVGLLISAFLAVFYCYIRKRCINLKSPYIICSGGSPL"
     exon            50..136
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /inference="alignment:Splign:2.1.0"
     exon            137..257
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /inference="alignment:Splign:2.1.0"
     exon            258..410
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /inference="alignment:Splign:2.1.0"
     exon            411..1704
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1680..1685
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /note="hexamer: AATAAA"
     polyA_site      1701
                     /gene="Epgn"
                     /gene_synonym="2310069M11Rik; epigen"
                     /note="major polyA site"
ORIGIN      
        1 cagaatatgg ctctgggggt tctgatagca gtctgcctct tgttcaaagc aatgaaggca
       61 gcactgagcg aagaagcaga ggtgatccct cctagcacag cacagcagag caactggaca
      121 tttaacaaca ccgaagctga ctacatagaa gaacctgtag ctctgaagtt ctctcatcct
      181 tgtctggaag accataatag ttactgcatt aatggagcat gtgcattcca ccatgagctg
      241 aagcaagcca tttgcagatg ctttactggt tatacgggac aacgatgtga gcatttgacc
      301 ctaacttcgt atgctgtgga ttcttatgaa aaatacattg cgattgggat tggcgtcgga
      361 ttgctaatta gtgcttttct tgctgtcttc tattgctaca taagaaaaag gtgtataaat
      421 ctgaaatcac cctacatcat ctgctctgga gggagcccat tgtgagacct tataagacat
      481 agtcatcaag ccatttgtca aaagccacag ggaatccaat ggagatcttt ggatgataca
      541 aaatgtgata agctaacttg aaaataatgg tggtttgggt cacaatgcag taattgacca
      601 ttggttctta gctttggtca tcgttgggtg ccatggaagc tatgggaatg agctacagta
      661 acagaagcca agttcactac ccttctttgg gtttgctgtt gggtggttgt tgtcactgca
      721 ggaagatttg ttctatactt ctgaccatct cagatgtgaa ttttcatttt aattgttttc
      781 tactacacat caatcaagtc caagtaatgc catttccggg ttcttcgggc actcaacatt
      841 ttgggccacc cgcctcgatg gacctaatag caaagtatct gtccttatgg aatttcaggg
      901 aatttggtat caatttttag atgaaaacag tgaatgtctc agctccttga gtgaaccaaa
      961 gatgcattac acctaaacca ctaaaagaaa atggaatatc caaggcagca taaatcctac
     1021 ccagctggtg acaacagttt gcaaacttca ttcatgtagt ttggaagaag cagataaatt
     1081 cctgaggact gaaagtcacc tggacagcag atccagagca ggcaaagtag ctgttcctat
     1141 atcgaccata aagcctgtgt gggctcatct gtcccctgat gtttttgcct atcatctcag
     1201 ccttacattg gaagactcac acttggtatc catcgcttga actgaagttc gacaattcac
     1261 ctaatgacta aaagcttaca attgttccca aaatatatag gaacaacagc atgtggaatg
     1321 taaccatttt ttgacgtgtt gatagcatat ttgcacatgg gttaaaaaaa gaaacagtcg
     1381 tagaaatact tattagggaa tcagtatccc tccttggaat tgcttctgct acatgattca
     1441 atcttgggca agtctcttat attctttgtg gtttggttcc attctctaca agacccatgc
     1501 agttccaaaa ttgaactcta atagaactaa aaaatacctc ctataactgc atggcaggca
     1561 agattatcct caatgcttcc atcctcagcc ccgtttctaa ccctcaaata cccacgaata
     1621 ttatccttac tatatattgt catgttcagt ttgtaaaata ataacttatt ttgaaaagaa
     1681 ataaaaaatg aaattacaaa gcaa
//
