LOCUS       NP_032558                315 aa            linear   ROD 11-MAR-2025
DEFINITION  epithelial cell adhesion molecule precursor [Mus musculus].
ACCESSION   NP_032558
VERSION     NP_032558.2
DBSOURCE    REFSEQ: accession NM_008532.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 315)
  AUTHORS   Hrncir,H.R., Goodloe,B., Bombin,S., Hogan,C.B., Jadi,O. and
            Gracz,A.D.
  TITLE     Sox9 inhibits Activin A to promote biliary maturation and branching
            morphogenesis
  JOURNAL   Nat Commun 16 (1), 1667 (2025)
   PUBMED   39955269
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 315)
  AUTHORS   Li,K.R., Yu,P.L., Zheng,Q.Q., Wang,X., Fang,X., Li,L.C. and Xu,C.R.
  TITLE     Spatiotemporal and genetic cell lineage tracing of endodermal
            organogenesis at single-cell resolution
  JOURNAL   Cell 188 (3), 796-813 (2025)
   PUBMED   39824184
REFERENCE   3  (residues 1 to 315)
  AUTHORS   Trinh,L.T., Finnel,R.R., Osipovich,A.B., Musselman,J.R.,
            Sampson,L.L., Wright,C.V.E. and Magnuson,M.A.
  TITLE     Positive autoregulation of Sox17 is necessary for gallbladder and
            extrahepatic bile duct formation
  JOURNAL   Development 152 (2) (2025)
   PUBMED   39745200
REFERENCE   4  (residues 1 to 315)
  AUTHORS   Defamie,V., Aliar,K., Sarkar,S., Vyas,F., Shetty,R., Reddy
            Narala,S., Fang,H., Saw,S., Tharmapalan,P., Sanchez,O., Knox,J.J.,
            Waterhouse,P.D. and Khokha,R.
  TITLE     Metalloproteinase inhibitors regulate biliary progenitor cells
            through sDLK1 in organoid models of liver injury
  JOURNAL   J Clin Invest 135 (3), e164997 (2024)
   PUBMED   39699962
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 315)
  AUTHORS   Cai,S. and Yin,N.
  TITLE     Single-cell transcriptome and chromatin accessibility mapping of
            upper lip and primary palate fusion
  JOURNAL   J Cell Mol Med 28 (19), e70128 (2024)
   PUBMED   39392189
REFERENCE   6  (residues 1 to 315)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   7  (residues 1 to 315)
  AUTHORS   Nelson,A.J., Dunn,R.J., Peach,R., Aruffo,A. and Farr,A.G.
  TITLE     The murine homolog of human Ep-CAM, a homotypic adhesion molecule,
            is expressed by thymocytes and thymic epithelial cells
  JOURNAL   Eur J Immunol 26 (2), 401-408 (1996)
   PUBMED   8617310
REFERENCE   8  (residues 1 to 315)
  AUTHORS   Borkowski,T.A., Nelson,A.J., Farr,A.G. and Udey,M.C.
  TITLE     Expression of gp40, the murine homologue of human epithelial cell
            adhesion molecule (Ep-CAM), by murine dendritic cells
  JOURNAL   Eur J Immunol 26 (1), 110-114 (1996)
   PUBMED   8566052
REFERENCE   9  (residues 1 to 315)
  AUTHORS   Bergsagel,P.L., Victor-Kobrin,C., Timblin,C.R., Trepel,J. and
            Kuehl,W.M.
  TITLE     A murine cDNA encodes a pan-epithelial glycoprotein that is also
            expressed on plasma cells
  JOURNAL   J Immunol 148 (2), 590-596 (1992)
   PUBMED   1729376
REFERENCE   10 (residues 1 to 315)
  AUTHORS   Timblin,C., Battey,J. and Kuehl,W.M.
  TITLE     Application for PCR technology to subtractive cDNA cloning:
            identification of genes expressed specifically in murine
            plasmacytoma cells
  JOURNAL   Nucleic Acids Res 18 (6), 1587-1593 (1990)
   PUBMED   2326198
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI986346.1, AK167182.1 and AK133510.1.
            
            On Aug 15, 2006 this sequence version replaced NP_032558.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AK133510.1, BC094465.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849376, SAMN01164133 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 57.87 cM"
     Protein         1..315
                     /product="epithelial cell adhesion molecule precursor"
                     /note="lymphocyte antigen 74; Trop-1 protein; mEGP314;
                     protein 289A; epithelial glycoprotein 314; panepithelial
                     glycoprotein 314; tumor-associated calcium signal
                     transducer 1"
                     /calculated_mol_wt=32867
     sig_peptide     1..23
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2171
     mat_peptide     24..315
                     /product="Epithelial cell adhesion molecule.
                     /id=PRO_0000380183"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99JW5.1)"
                     /calculated_mol_wt=32867
     Region          29..61
                     /region_name="EpCAM_N"
                     /note="Epithelial cell adhesion molecule N-terminal
                     domain; pfam18635"
                     /db_xref="CDD:465828"
     Region          66..131
                     /region_name="Thyroglobulin_1"
                     /note="Thyroglobulin type-1 repeat; pfam00086"
                     /db_xref="CDD:459665"
     Site            order(70,94,112,115)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Site            111
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q99JW5.1)"
     Site            198
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q99JW5.1)"
     Site            267..289
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99JW5.1)"
     CDS             1..315
                     /gene="Epcam"
                     /gene_synonym="CD326; EGP; EGP-2; Egp314; Ep-CAM; EpCAM1;
                     GA733-2; gp40; Ly74; Tacsd1; Tacstd1; TROP1"
                     /coded_by="NM_008532.2:456..1403"
                     /db_xref="CCDS:CCDS29018.1"
                     /db_xref="GeneID:17075"
                     /db_xref="MGI:MGI:106653"
ORIGIN      
        1 magpqalafg lllavvtatl aaaqrdcvcd nyklatscsl neygecqcts ygtqntvics
       61 klaskclamk aemthsksgr rikpegaiqn ndglydpdcd eqglfkakqc ngtatcwcvn
      121 tagvrrtdkd teitcservr tywiiielkh kerespydhq slqtalqeaf tsryklnqkf
      181 iknimyennv itidlmqnss qktqddvdia dvayyfekdv kgeslfhssk smdlrvngep
      241 ldldpgqtli yyvdekapef smqgltagii avivvvslav iagivvlvis trkksakyek
      301 aeikemgeih relna
//
