LOCUS       NM_183428               6565 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus erythrocyte membrane protein band 4.1 (Epb41),
            transcript variant 1, mRNA.
ACCESSION   NM_183428 XM_194176
VERSION     NM_183428.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Lu,Y., Fan,D., Wang,W., Gao,X., Li,H., Guo,S., Zhao,L., Guo,Y.,
            Li,B., Zhong,Y., Zhang,B., Liu,J., Dai,L., Kang,Q. and Ji,Z.
  TITLE     The protein 4.1R downregulates VEGFA in M2 macrophages to inhibit
            colon cancer metastasis
  JOURNAL   Exp Cell Res 409 (1), 112896 (2021)
   PUBMED   34717920
  REMARK    GeneRIF: The protein 4.1R downregulates VEGFA in M2 macrophages to
            inhibit colon cancer metastasis.
REFERENCE   3  (bases 1 to 6565)
  AUTHORS   Huang,S.C., Vu,L.V., Yu,F.H., Nguyen,D.T. and Benz,E.J. Jr.
  TITLE     Multifunctional protein 4.1R regulates the asymmetric segregation
            of Numb during terminal erythroid maturation
  JOURNAL   J Biol Chem 297 (3), 101051 (2021)
   PUBMED   34364872
  REMARK    GeneRIF: Multifunctional protein 4.1R regulates the asymmetric
            segregation of Numb during terminal erythroid maturation.
REFERENCE   4  (bases 1 to 6565)
  AUTHORS   Li,B., Zhang,X., Lu,Y., Zhao,L., Guo,Y., Guo,S., Kang,Q., Liu,J.,
            Dai,L., Zhang,L., Fan,D. and Ji,Z.
  TITLE     Protein 4.1R affects photodynamic therapy for B16 melanoma by
            regulating the transport of 5-aminolevulinic acid
  JOURNAL   Exp Cell Res 399 (2), 112465 (2021)
   PUBMED   33385415
  REMARK    GeneRIF: Protein 4.1R affects photodynamic therapy for B16 melanoma
            by regulating the transport of 5-aminolevulinic acid.
REFERENCE   5  (bases 1 to 6565)
  AUTHORS   Ding,C., Guo,Y., Liang,T., Liu,J., Yang,L., Wang,T., Liu,X. and
            Kang,Q.
  TITLE     Protein 4.1R negatively regulates P815 cells proliferation by
            inhibiting C-Kit-mediated signal transduction
  JOURNAL   Exp Cell Res 398 (2), 112403 (2021)
   PUBMED   33271128
  REMARK    GeneRIF: Protein 4.1R negatively regulates P815 cells proliferation
            by inhibiting C-Kit-mediated signal transduction.
REFERENCE   6  (bases 1 to 6565)
  AUTHORS   Winardi,R., Discher,D., Kelley,C., Zon,L., Mays,K., Mohandas,N. and
            Conboy,J.G.
  TITLE     Evolutionarily conserved alternative pre-mRNA splicing regulates
            structure and function of the spectrin-actin binding domain of
            erythroid protein 4.1
  JOURNAL   Blood 86 (11), 4315-4322 (1995)
   PUBMED   7492792
REFERENCE   7  (bases 1 to 6565)
  AUTHORS   Takeuchi,K., Kawashima,A., Nagafuchi,A. and Tsukita,S.
  TITLE     Structural diversity of band 4.1 superfamily members
  JOURNAL   J Cell Sci 107 (Pt 7), 1921-1928 (1994)
   PUBMED   7983158
REFERENCE   8  (bases 1 to 6565)
  AUTHORS   Bahary,N., Zorich,G., Pachter,J.E., Leibel,R.L. and Friedman,J.M.
  TITLE     Molecular genetic linkage maps of mouse chromosomes 4 and 6
  JOURNAL   Genomics 11 (1), 33-47 (1991)
   PUBMED   1684952
REFERENCE   9  (bases 1 to 6565)
  AUTHORS   Conboy,J., Kan,Y.W., Shohet,S.B. and Mohandas,N.
  TITLE     Molecular cloning of protein 4.1, a major structural element of the
            human erythrocyte membrane skeleton
  JOURNAL   Proc Natl Acad Sci U S A 83 (24), 9512-9516 (1986)
   PUBMED   3467321
REFERENCE   10 (bases 1 to 6565)
  AUTHORS   Conboy,J., Mohandas,N., Tchernia,G. and Kan,Y.W.
  TITLE     Molecular basis of hereditary elliptocytosis due to protein 4.1
            deficiency
  JOURNAL   N Engl J Med 315 (11), 680-685 (1986)
   PUBMED   3755799
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669981.11, CB723734.1, CN537562.1, CN526385.1, CB526050.1 and
            AK220462.1.
            
            On Jun 19, 2008 this sequence version replaced NM_183428.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq was created from transcript and genomic
            sequence data because transcript sequence consistent with the
            reference genome assembly was not available for all regions of the
            RefSeq transcript, The extent of this transcript is supported by
            transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164138,
                              SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1470              AL669981.11        117335-118804       c
            1471-2158           CB723734.1         38-725
            2159-2621           CN537562.1         215-677
            2622-3169           CN526385.1         204-751
            3170-3663           CB526050.1         113-606
            3664-6565           AK220462.1         2147-5048
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 64.54 cM"
     gene            1..6565
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="erythrocyte membrane protein band 4.1"
                     /db_xref="GeneID:269587"
                     /db_xref="MGI:MGI:95401"
     exon            1..1470
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1951
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     CDS             1478..4054
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein 4.1; P4.1; band 4.1; Protein 4.1R; erythrocyte
                     protein band 4.1"
                     /codon_start=1
                     /product="protein 4.1 isoform 1"
                     /protein_id="NP_906273.3"
                     /db_xref="CCDS:CCDS18717.1"
                     /db_xref="GeneID:269587"
                     /db_xref="MGI:MGI:95401"
                     /translation="MTTEKSLAAEAENSQHQQQKEEGEGATNSGQQETQLEEASQAAA
                     AEGSDQGEQKLKASNGDTPTHEDLTKNKERTSESRGLSRLLSSFLKRPKSQVSEEEGR
                     EVESEKEKGEGGQKEIELGNSLDEDIILKAPIAAPEPELKTDPSLDLHSLSSIETQPA
                     QEEHREDPDSETKEGEGIEECSGTEVKEDPESRAEREPEASQKPVRRHRNMHCKVSLL
                     DDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLALWDSATSKTWLDSAKEIKKQV
                     RGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTI
                     QSELGDYDPELHGMDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAK
                     KLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFI
                     KIRPGEQEHYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLTSTDTIPKSKFLALGSKF
                     RYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAAESTDRSPRPTSAPAIA
                     QSQVTEGPGAPIKKTPKEAVKVEEKRGEEPAEPAEPEPTEAWKVEKTHTEVTVPTSNG
                     DQTQKLAGKGEDLIRMRKKKRERLDGENIYIRHSNLMLEDLDKSQEEIKKHHASISEL
                     KKNFMESVPEPRPSEWDKRLSTHSPFRTLNINGQVPTGDGPPLVKTQTVTISDTANAV
                     KSEIPTKDVPIVHTETKTITYEAAQTEDSNGDLDPGVLLTAQTITSETTSSTTTTQIT
                     KTVKGGISETRIEKRIVITGDADIDHDQVLVQAIKEAKEQHPDMSVTKVVVHQETEIS
                     EE"
     exon            1952..2161
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2266
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2309
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2385
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2604
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2605..2692
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2845
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..2943
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2944..3116
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..3304
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3305..3361
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3362..3403
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3404..3466
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3467..3643
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3644..3772
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3773..3874
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3875..3955
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            3956..4060
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            4061..6565
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4636..4641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="hexamer: AAGAAA"
     polyA_site      4664
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="major polyA site"
ORIGIN      
        1 agatagggga cgcctggagc ccactgatat ttgttcacag agccatatct acaggtccaa
       61 gagctgggca ttaaaaagta atattgagtc cactttgggc ctttgcttcc tcatctataa
      121 aaggtggatt tgagtgaatg cagatgtccc tggatgcttg agtgattact ggctgaggtc
      181 agctgtagga acagaccagg ggtcaaggtg ccaatgactg agccaggaga ggtggcttcc
      241 agagtagctt gcaccctaag ctggctgttt gacaagaaga gtttcagatg aaagtattca
      301 aagacttgct ggaggcagtg cagatgtact gcagagacca aggggaagaa atgaaggaaa
      361 gtttcgagca cataatccaa gcagacatga gctttggtca cttcactcgg agacccatat
      421 gatggcacca aggacacaga agggagtccc ttcgcccttg cccttgtctg tgaagtggga
      481 acagtaacaa tttcacctac acagtgttgg ggagactgtg aagaataaag tggaatatct
      541 gtaaaattgg gagaatatgg tggctggtca tttttcaatc caaagctgca gtactgggtg
      601 tgcgtgtggg gaggggcact tgtcatagcc ctgatgggac tctgcatggt gagaatgtgc
      661 tgggggacac cttaaggtca cagcagtact tacagactca tcacagtgac cagacattgt
      721 gctgggactt cgtttttcat tatctccctt ggtcccaaca gcagcattct tttgagaaga
      781 tggtggtctc acccacacta gatcctggca aagtccccat ccacagcccc ttgtagtcat
      841 agtagctaca agttataaac aaatgatata ccaacctctg ttcaaaaagt cttacatatg
      901 tcaactctta catgactccc acgtagcccg aagcggcaga tctgaatgct gccctctatg
      961 ttacgggtaa gtagaggaag cctggaggga gaagcgaggg aatcgcctga gtggcaccag
     1021 tggtagggct gtttgtggag gcaggatttg acccccagta tctaaggtca aatgcatgca
     1081 tcactgaagt tttactcccc cgcctccata ctgcagtgta gggctcacat gggcatggcc
     1141 ctgtctgact tcagacggca tggcgcctgg tgcggctatg aatgcccata ggagactggg
     1201 cccagggcag cctgatgagc tcagcacaga ttttcagatc atagctgcct gaatggtatc
     1261 tggtcatcag agtgccagaa gcactctggg tggggggtga gggcggaaga ggggacaggt
     1321 tccccactgg cccaggtgca gctgatcagg accccagagt ttgatagctg tgctgtgtct
     1381 cacttctgtc ctttgcttcc atgtgccacc gtgacgatgg ctgcgctgtc cttccttgac
     1441 tggctgtatg atgacaacgg ctacttctgg gaacatcatg acaacagaga agagtttagc
     1501 ggctgaagct gagaattctc agcaccagca acagaaggaa gaaggagagg gagccacaaa
     1561 ctcaggccaa caagaaactc agctggagga ggcctcccaa gcggccgcag ctgagggcag
     1621 cgatcagggt gagcagaagc tgaaagcatc caatggggac actccaacac atgaagactt
     1681 gaccaagaac aaggaacgga catcagaaag cagaggcctg tcacgactgc tctcctcatt
     1741 cctcaaaagg cccaagtcgc aggtctctga agaagaaggc agagaagtag aatcagagaa
     1801 agagaaaggt gaagggggtc agaaggagat agaacttgga aacagccttg atgaagacat
     1861 cattttaaag gcccccattg cagctcctga acctgagctc aaaacagacc catctttgga
     1921 tcttcattca ttaagcagta tagagacaca gccagctcag gaagaacaca gagaggaccc
     1981 agattctgaa acgaaggaag gagaagggat tgaagagtgc tctggaacag aggtgaaaga
     2041 ggacccagag tcaagagcag agagagaacc cgaagcctcc cagaagcccg tcagaagaca
     2101 cagaaacatg cactgtaagg tctccttgtt ggatgacacg gtctacgaat gtgttgtaga
     2161 gaaacatgct aagggacaag acttgctgaa gcgagtgtgc gagcacctca accttttgga
     2221 agaagactac tttggtttag ccctgtggga cagcgcaacc tctaagacat ggctggattc
     2281 tgccaaagaa ataaaaaagc aggttcgagg tgttccttgg aatttcacat ttaatgtgaa
     2341 gttttatcca cccgacccag cacaattaac agaagacata acaagatact atttatgtct
     2401 tcagcttcgg caggacattg ttgctggacg tctgccctgt tcctttgcaa ctttagccct
     2461 actcgggtct tacacgatcc agtctgagct gggagactat gacccagaac tgcacggcat
     2521 ggattatgtt agtgatttta aactggctcc aaatcagacc aaggaacttg aagaaaaggt
     2581 catggaattg cataaatcat acaggtccat gactccagct caggctgact tggaatttct
     2641 tgagaatgcc aaaaagttgt ccatgtatgg agttgatctt cacaaagcaa aggacttgga
     2701 gggagtggac attattctcg gcgtctgctc cagcggcctt ctggtttaca aagacaagtt
     2761 gagaattaac cgctttcctt ggcccaaagt gctaaagatt tcttacaaac gcagcagctt
     2821 cttcatcaag atccggcctg gagagcaaga acattatgaa agtaccatcg gcttcaagct
     2881 ccccagttat cgagcggcca agaaactatg gaaggtctgt gtggagcatc acacgttctt
     2941 cagactcacc tctacagaca ccatccccaa aagcaagttt cttgccctgg gatccaaatt
     3001 ccgatacagt ggccggactc aagctcagac caggcaagcc agtgccctga ttgacaggcc
     3061 tgctccacac tttgagcgga cagcaagcaa gcgggcgtcc aggagcctcg atggagcagc
     3121 agctgctgag tccacagacc gaagtcctcg gcccacctct gccccagcca ttgctcagag
     3181 tcaggtcaca gaagggccag gggcacctat caaaaaaaca ccaaaggaag ctgtgaaggt
     3241 tgaagagaag cggggagaag agccggctga gcccgctgag ccggagccca cagaagcatg
     3301 gaaggtggaa aaaacccaca ccgaggtcac agtacctacc tcaaatggtg accaaacgca
     3361 gaagcttgca ggaaaaggtg aagatctgat aagaatgagg aagaaaaaga gagagagact
     3421 agatggtgaa aacatttata tcagacatag caatttaatg ttggaggatt tagacaagag
     3481 tcaagaagag atcaaaaagc accatgccag catcagtgaa ctgaaaaaga actttatgga
     3541 atcggtaccc gaaccacggc ccagcgagtg ggacaagcgc ttatctacac actcaccctt
     3601 ccggactctt aacatcaacg ggcaagtccc tactggagat ggacctcctc tggtaaagac
     3661 tcaaactgtc accatctcag atactgccaa tgctgtgaaa agtgaaatcc caaccaaaga
     3721 tgtccctatt gtccacactg agaccaagac catcacctat gaagctgccc agactgagga
     3781 cagcaatggg gacttagacc ctggagtctt gctgacagcc cagaccatca catccgagac
     3841 cacaagtagt acaaccacga cacagattac caagactgta aaaggtggga tttctgagac
     3901 ccggatcgag aagagaattg tgatcacagg agatgccgat atcgaccatg atcaggtcct
     3961 tgtacaagcc atcaaggaag ccaaggagca gcatccagac atgtcagtga ccaaggtggt
     4021 cgtccaccag gagacagaga tctctgagga gtgagctcag ggactgtcct gccccacccc
     4081 cacctgtccc aatccaagag aagccagcat gtgataaaga agccagcctg cagcagactt
     4141 cacactttga agactgttcc acaccacaga aaaccaactt cattttctac tcagaattta
     4201 taccaagaga ctcttgtaga cgccgcggat gtcctctata ttgtgataca gaaactgtca
     4261 acttttcgct ctgtgaactg tttttaagac actttgatgt ttttatgggg tggttggtaa
     4321 cctctcagcc tagcctctcc tcactcattt tcaacgctga ccctgacagg atccacagac
     4381 ttctccagga ccttccagag accctgccag ctgttagagg acagagccag ctcagtagga
     4441 cctcctgctc ctccccccgg cttcgtgccc tttggatgac acaaaaactt catacaccag
     4501 tgactgtcgg gcagggcaag cgccctggtc acagacccca gaaacatgac ctcccactgc
     4561 agggtgttga aggccgagcc ctccctccgc tgagcatgtg atgtcacccc ctctgtttgg
     4621 agcttcttta ggtcaaagaa atcaattgct ttccatagag ctgatactgt gaacattcga
     4681 atgcatctgt ggccttcgcc tccccagctg ccctgtccct ccttggaaac agcagctttg
     4741 ccgagggctc cccagcttcc atctcaggag accagaactc atggaaaaat taggcacttt
     4801 tggctaaaag tgcgaatgga acatttttag ttgtagtctg gggaagaaaa gttaatgagg
     4861 ttttaaaaat caggtcttgt cgtgtgggga gcttgctccc ccaggggaga ggccccttta
     4921 cctcagtgga tcctgttaag gagtgcttcc agcctcagcc tgggcccctc ccacctggca
     4981 tggtaagggg gtaagggacg ctcagggaag aggtgcattc tccgacctct gcctcaggcc
     5041 tgaggacgcc cactgggagc tggggcattt ccatctcctg gtggccatcc ctggtcaggg
     5101 tgtgagtgag ctctgctact ccccacccag tgcctgagga gcctgtcagg ttttgagagc
     5161 ttgattttat ggtgggtttt ggatttagtt tcctttttcc ttttttaaat attggtttta
     5221 aaaagggagc ctgatgcccc caattggtag tgagaatttg gggacctccc caacccccga
     5281 ccccacaaac agggcctctg taatcctcca gctaacttcc tacaaacatc tgcctggaag
     5341 attagtagaa tcctaattct aggctggaat gagcttcatg aggcttgaca gaggctcact
     5401 gtcagccgtg gctgggggct gaaggggagt aagcaagagc aaagtgggaa gccacggcag
     5461 cctccggcct cccctgctga atgctctata caaattcagc acctttgact ggttttgagt
     5521 gtgcattttc ttttagtgat actgttacca gtggcagttt gctttacaga gggggagccc
     5581 aggtgggaga gaaatgggct tgggttccat tctcagaagt gaattgcaga ctggaactga
     5641 gccagagaag gctgggctgt aggggttgga ttagcatggg ctcaagctac accagctctg
     5701 aagaggagga gcaggaggag cctgcccact gggcatcgtg ccatccccat caggacctca
     5761 gacctggggt acctgaactc tgtccctctg ctgccgcctc tgtctggaaa ttggaccaga
     5821 attctgatgt ccctccagcc tcctccccct cctctgctgg ctctggcaac agatcctggg
     5881 atatggtaat tgtttggatt ttctttcttt ctgcagttgt gtgtgaagaa caggctctgt
     5941 ccaggtagaa agattgaggg gcagggggag gggagtccca tctccagggt cagagacttg
     6001 gcaggacttt ccccaaagtg actcaggcac accacagtaa cagtcccaac tgcagtttta
     6061 tttttacttc agaagtagat ttccttcaaa ccacatgaga tctctgccac ccacccacaa
     6121 tcaagcccta tattcccaag gaggctgact ccgggggccc ttccccccag gccagcaatg
     6181 tattccgggg atgtattctg gggtctgagg gccctggccc attctgagaa atggagctgt
     6241 agctatggcc tagccagcac ccccagtgct cgctcatggg aaatatcagc cctaggctgg
     6301 ttgtttttac aaatctctct cagatatatt attttcatga caaaaatgaa ggagctttgt
     6361 aaatttttta aaaattatga atcgtatcaa gtagttgttt acatttgaca aaataggaac
     6421 tatgccggca gagtccaacc gaaaaccccc taggtgagat aggcagtaac tgggcctgct
     6481 gtctgcgcct ttcgtagtga cagatgtggc tgtagtgttt aggtgtttgg tcttgcttct
     6541 tgtatggcat ttttcaataa acttt
//
