LOCUS       NM_001128607            5066 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus erythrocyte membrane protein band 4.1 (Epb41),
            transcript variant 3, mRNA.
ACCESSION   NM_001128607
VERSION     NM_001128607.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Lu,Y., Fan,D., Wang,W., Gao,X., Li,H., Guo,S., Zhao,L., Guo,Y.,
            Li,B., Zhong,Y., Zhang,B., Liu,J., Dai,L., Kang,Q. and Ji,Z.
  TITLE     The protein 4.1R downregulates VEGFA in M2 macrophages to inhibit
            colon cancer metastasis
  JOURNAL   Exp Cell Res 409 (1), 112896 (2021)
   PUBMED   34717920
  REMARK    GeneRIF: The protein 4.1R downregulates VEGFA in M2 macrophages to
            inhibit colon cancer metastasis.
REFERENCE   3  (bases 1 to 5066)
  AUTHORS   Huang,S.C., Vu,L.V., Yu,F.H., Nguyen,D.T. and Benz,E.J. Jr.
  TITLE     Multifunctional protein 4.1R regulates the asymmetric segregation
            of Numb during terminal erythroid maturation
  JOURNAL   J Biol Chem 297 (3), 101051 (2021)
   PUBMED   34364872
  REMARK    GeneRIF: Multifunctional protein 4.1R regulates the asymmetric
            segregation of Numb during terminal erythroid maturation.
REFERENCE   4  (bases 1 to 5066)
  AUTHORS   Li,B., Zhang,X., Lu,Y., Zhao,L., Guo,Y., Guo,S., Kang,Q., Liu,J.,
            Dai,L., Zhang,L., Fan,D. and Ji,Z.
  TITLE     Protein 4.1R affects photodynamic therapy for B16 melanoma by
            regulating the transport of 5-aminolevulinic acid
  JOURNAL   Exp Cell Res 399 (2), 112465 (2021)
   PUBMED   33385415
  REMARK    GeneRIF: Protein 4.1R affects photodynamic therapy for B16 melanoma
            by regulating the transport of 5-aminolevulinic acid.
REFERENCE   5  (bases 1 to 5066)
  AUTHORS   Ding,C., Guo,Y., Liang,T., Liu,J., Yang,L., Wang,T., Liu,X. and
            Kang,Q.
  TITLE     Protein 4.1R negatively regulates P815 cells proliferation by
            inhibiting C-Kit-mediated signal transduction
  JOURNAL   Exp Cell Res 398 (2), 112403 (2021)
   PUBMED   33271128
  REMARK    GeneRIF: Protein 4.1R negatively regulates P815 cells proliferation
            by inhibiting C-Kit-mediated signal transduction.
REFERENCE   6  (bases 1 to 5066)
  AUTHORS   Winardi,R., Discher,D., Kelley,C., Zon,L., Mays,K., Mohandas,N. and
            Conboy,J.G.
  TITLE     Evolutionarily conserved alternative pre-mRNA splicing regulates
            structure and function of the spectrin-actin binding domain of
            erythroid protein 4.1
  JOURNAL   Blood 86 (11), 4315-4322 (1995)
   PUBMED   7492792
REFERENCE   7  (bases 1 to 5066)
  AUTHORS   Takeuchi,K., Kawashima,A., Nagafuchi,A. and Tsukita,S.
  TITLE     Structural diversity of band 4.1 superfamily members
  JOURNAL   J Cell Sci 107 (Pt 7), 1921-1928 (1994)
   PUBMED   7983158
REFERENCE   8  (bases 1 to 5066)
  AUTHORS   Bahary,N., Zorich,G., Pachter,J.E., Leibel,R.L. and Friedman,J.M.
  TITLE     Molecular genetic linkage maps of mouse chromosomes 4 and 6
  JOURNAL   Genomics 11 (1), 33-47 (1991)
   PUBMED   1684952
REFERENCE   9  (bases 1 to 5066)
  AUTHORS   Conboy,J., Kan,Y.W., Shohet,S.B. and Mohandas,N.
  TITLE     Molecular cloning of protein 4.1, a major structural element of the
            human erythrocyte membrane skeleton
  JOURNAL   Proc Natl Acad Sci U S A 83 (24), 9512-9516 (1986)
   PUBMED   3467321
REFERENCE   10 (bases 1 to 5066)
  AUTHORS   Conboy,J., Mohandas,N., Tchernia,G. and Kan,Y.W.
  TITLE     Molecular basis of hereditary elliptocytosis due to protein 4.1
            deficiency
  JOURNAL   N Engl J Med 315 (11), 680-685 (1986)
   PUBMED   3755799
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669981.11 and AL607088.26.
            
            On Aug 21, 2018 this sequence version replaced NM_001128607.1.
            
            Transcript Variant: This variant (3) lacks multiple in-frame exons,
            uses an alternate 5' splice site, and uses a different 5' UTR,
            compared to variant 1. These differences cause translation
            initiation at a downstream ATG and an isoform (3) that is shorter
            than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068138.1, SRR9219381.196460.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AL669981.11        121887-122020       c
            135-598             AL669981.11        53665-54128         c
            599-808             AL669981.11        50292-50501         c
            809-913             AL669981.11        46907-47011         c
            914-956             AL669981.11        44206-44248         c
            957-1032            AL669981.11        37918-37993         c
            1033-1251           AL669981.11        36422-36640         c
            1252-1339           AL669981.11        29160-29247         c
            1340-1492           AL669981.11        25517-25669         c
            1493-1590           AL669981.11        22245-22342         c
            1591-1763           AL669981.11        21391-21563         c
            1764-1951           AL669981.11        14495-14682         c
            1952-2128           AL669981.11        4350-4526           c
            2129-2257           AL607088.26        84371-84499         c
            2258-2359           AL607088.26        78326-78427         c
            2360-2440           AL607088.26        77124-77204         c
            2441-2545           AL607088.26        75136-75240         c
            2546-5066           AL607088.26        72045-74565         c
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 64.54 cM"
     gene            1..5066
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="erythrocyte membrane protein band 4.1"
                     /db_xref="GeneID:269587"
                     /db_xref="MGI:MGI:95401"
     exon            1..134
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            135..598
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            599..808
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     CDS             755..2539
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="isoform 3 is encoded by transcript variant 3;
                     protein 4.1; P4.1; band 4.1; Protein 4.1R; erythrocyte
                     protein band 4.1"
                     /codon_start=1
                     /product="protein 4.1 isoform 3"
                     /protein_id="NP_001122079.1"
                     /db_xref="CCDS:CCDS89834.1"
                     /db_xref="GeneID:269587"
                     /db_xref="MGI:MGI:95401"
                     /translation="MHCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGL
                     ALWDSATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQ
                     DIVAGRLPCSFATLALLGSYTIQSELGDYDPELHGMDYVSDFKLAPNQTKELEEKVME
                     LHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKL
                     RINRFPWPKVLKISYKRSSFFIKIRPGEQEHYESTIGFKLPSYRAAKKLWKVCVEHHT
                     FFRLTSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSL
                     DGAAAAESTDRSPRPTSAPAIAQSQVTEGPGAPIKKTPKEAVKVEEKRGEEPAEPAEP
                     EPTEAWKDLDKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTLNIN
                     GQVPTGDGPPLVKTQTVTISDTANAVKSEIPTKDVPIVHTETKTITYEAAQTEDSNGD
                     LDPGVLLTAQTITSETTSSTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQVLVQ
                     AIKEAKEQHPDMSVTKVVVHQETEISEE"
     exon            809..913
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            914..956
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1032
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1251
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1339
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1492
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1590
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1763
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1951
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2128
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2257
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2359
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2440
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2545
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..5066
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3121..3126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="hexamer: AAGAAA"
     polyA_site      3149
                     /gene="Epb41"
                     /gene_synonym="4.1R; D4Ertd442e; Elp-1; Elp1; Epb4.1;
                     mKIAA4056"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtgcag gtggaggccc ccggcggggc aaagtggcag gaacctctta aagggagaga
       61 gccgcggaga gcgaggacgc ggcccggccc ggcccggcca ggtccctgcc aggtccctgt
      121 cctgtgcagc ccagagaaga gtttagcggc tgaagctgag aattctcagc accagcaaca
      181 gaaggaagaa ggagagggag ccacaaactc aggccaacaa gaaactcagc tggaggaggc
      241 ctcccaagcg gccgcagctg agggcagcga tcagggtgag cagaagctga aagcatccaa
      301 tggggacact ccaacacatg aagacttgac caagaacaag gaacggacat cagaaagcag
      361 aggcctgtca cgactgctct cctcattcct caaaaggccc aagtcgcagg tctctgaaga
      421 agaaggcaga gaagtagaat cagagaaaga gaaaggtgaa gggggtcaga aggagataga
      481 acttggaaac agccttgatg aagacatcat tttaaaggcc cccattgcag ctcctgaacc
      541 tgagctcaaa acagacccat ctttggatct tcattcatta agcagtatag agacacagcc
      601 agctcaggaa gaacacagag aggacccaga ttctgaaacg aaggaaggag aagggattga
      661 agagtgctct ggaacagagg tgaaagagga cccagagtca agagcagaga gagaacccga
      721 agcctcccag aagcccgtca gaagacacag aaacatgcac tgtaaggtct ccttgttgga
      781 tgacacggtc tacgaatgtg ttgtagagaa acatgctaag ggacaagact tgctgaagcg
      841 agtgtgcgag cacctcaacc ttttggaaga agactacttt ggtttagccc tgtgggacag
      901 cgcaacctct aagacatggc tggattctgc caaagaaata aaaaagcagg ttcgaggtgt
      961 tccttggaat ttcacattta atgtgaagtt ttatccaccc gacccagcac aattaacaga
     1021 agacataaca agatactatt tatgtcttca gcttcggcag gacattgttg ctggacgtct
     1081 gccctgttcc tttgcaactt tagccctact cgggtcttac acgatccagt ctgagctggg
     1141 agactatgac ccagaactgc acggcatgga ttatgttagt gattttaaac tggctccaaa
     1201 tcagaccaag gaacttgaag aaaaggtcat ggaattgcat aaatcataca ggtccatgac
     1261 tccagctcag gctgacttgg aatttcttga gaatgccaaa aagttgtcca tgtatggagt
     1321 tgatcttcac aaagcaaagg acttggaggg agtggacatt attctcggcg tctgctccag
     1381 cggccttctg gtttacaaag acaagttgag aattaaccgc tttccttggc ccaaagtgct
     1441 aaagatttct tacaaacgca gcagcttctt catcaagatc cggcctggag agcaagaaca
     1501 ttatgaaagt accatcggct tcaagctccc cagttatcga gcggccaaga aactatggaa
     1561 ggtctgtgtg gagcatcaca cgttcttcag actcacctct acagacacca tccccaaaag
     1621 caagtttctt gccctgggat ccaaattccg atacagtggc cggactcaag ctcagaccag
     1681 gcaagccagt gccctgattg acaggcctgc tccacacttt gagcggacag caagcaagcg
     1741 ggcgtccagg agcctcgatg gagcagcagc tgctgagtcc acagaccgaa gtcctcggcc
     1801 cacctctgcc ccagccattg ctcagagtca ggtcacagaa gggccagggg cacctatcaa
     1861 aaaaacacca aaggaagctg tgaaggttga agagaagcgg ggagaagagc cggctgagcc
     1921 cgctgagccg gagcccacag aagcatggaa ggatttagac aagagtcaag aagagatcaa
     1981 aaagcaccat gccagcatca gtgaactgaa aaagaacttt atggaatcgg tacccgaacc
     2041 acggcccagc gagtgggaca agcgcttatc tacacactca cccttccgga ctcttaacat
     2101 caacgggcaa gtccctactg gagatggacc tcctctggta aagactcaaa ctgtcaccat
     2161 ctcagatact gccaatgctg tgaaaagtga aatcccaacc aaagatgtcc ctattgtcca
     2221 cactgagacc aagaccatca cctatgaagc tgcccagact gaggacagca atggggactt
     2281 agaccctgga gtcttgctga cagcccagac catcacatcc gagaccacaa gtagtacaac
     2341 cacgacacag attaccaaga ctgtaaaagg tgggatttct gagacccgga tcgagaagag
     2401 aattgtgatc acaggagatg ccgatatcga ccatgatcag gtccttgtac aagccatcaa
     2461 ggaagccaag gagcagcatc cagacatgtc agtgaccaag gtggtcgtcc accaggagac
     2521 agagatctct gaggagtgag ctcagggact gtcctgcccc acccccacct gtcccaatcc
     2581 aagagaagcc agcatgtgat aaagaagcca gcctgcagca gacttcacac tttgaagact
     2641 gttccacacc acagaaaacc aacttcattt tctactcaga atttatacca agagactctt
     2701 gtagacgccg cggatgtcct ctatattgtg atacagaaac tgtcaacttt tcgctctgtg
     2761 aactgttttt aagacacttt gatgttttta tggggtggtt ggtaacctct cagcctagcc
     2821 tctcctcact cattttcaac gctgaccctg acaggatcca cagacttctc caggaccttc
     2881 cagagaccct gccagctgtt agaggacaga gccagctcag taggacctcc tgctcctccc
     2941 cccggcttcg tgccctttgg atgacacaaa aacttcatac accagtgact gtcgggcagg
     3001 gcaagcgccc tggtcacaga ccccagaaac atgacctccc actgcagggt gttgaaggcc
     3061 gagccctccc tccgctgagc atgtgatgtc accccctctg tttggagctt ctttaggtca
     3121 aagaaatcaa ttgctttcca tagagctgat actgtgaaca ttcgaatgca tctgtggcct
     3181 tcgcctcccc agctgccctg tccctccttg gaaacagcag ctttgccgag ggctccccag
     3241 cttccatctc aggagaccag aactcatgga aaaattaggc acttttggct aaaagtgcga
     3301 atggaacatt tttagttgta gtctggggaa gaaaagttaa tgaggtttta aaaatcaggt
     3361 cttgtcgtgt ggggagcttg ctcccccagg ggagaggccc ctttacctca gtggatcctg
     3421 ttaaggagtg cttccagcct cagcctgggc ccctcccacc tggcatggta agggggtaag
     3481 ggacgctcag ggaagaggtg cattctccga cctctgcctc aggcctgagg acgcccactg
     3541 ggagctgggg catttccatc tcctggtggc catccctggt cagggtgtga gtgagctctg
     3601 ctactcccca cccagtgcct gaggagcctg tcaggttttg agagcttgat tttatggtgg
     3661 gttttggatt tagtttcctt tttccttttt taaatattgg ttttaaaaag ggagcctgat
     3721 gcccccaatt ggtagtgaga atttggggac ctccccaacc cccgacccca caaacagggc
     3781 ctctgtaatc ctccagctaa cttcctacaa acatctgcct ggaagattag tagaatccta
     3841 attctaggct ggaatgagct tcatgaggct tgacagaggc tcactgtcag ccgtggctgg
     3901 gggctgaagg ggagtaagca agagcaaagt gggaagccac ggcagcctcc ggcctcccct
     3961 gctgaatgct ctatacaaat tcagcacctt tgactggttt tgagtgtgca ttttctttta
     4021 gtgatactgt taccagtggc agtttgcttt acagaggggg agcccaggtg ggagagaaat
     4081 gggcttgggt tccattctca gaagtgaatt gcagactgga actgagccag agaaggctgg
     4141 gctgtagggg ttggattagc atgggctcaa gctacaccag ctctgaagag gaggagcagg
     4201 aggagcctgc ccactgggca tcgtgccatc cccatcagga cctcagacct ggggtacctg
     4261 aactctgtcc ctctgctgcc gcctctgtct ggaaattgga ccagaattct gatgtccctc
     4321 cagcctcctc cccctcctct gctggctctg gcaacagatc ctgggatatg gtaattgttt
     4381 ggattttctt tctttctgca gttgtgtgtg aagaacaggc tctgtccagg tagaaagatt
     4441 gaggggcagg gggaggggag tcccatctcc agggtcagag acttggcagg actttcccca
     4501 aagtgactca ggcacaccac agtaacagtc ccaactgcag ttttattttt acttcagaag
     4561 tagatttcct tcaaaccaca tgagatctct gccacccacc cacaatcaag ccctatattc
     4621 ccaaggaggc tgactccggg ggcccttccc cccaggccag caatgtattc cggggatgta
     4681 ttctggggtc tgagggccct ggcccattct gagaaatgga gctgtagcta tggcctagcc
     4741 agcaccccca gtgctcgctc atgggaaata tcagccctag gctggttgtt tttacaaatc
     4801 tctctcagat atattatttt catgacaaaa atgaaggagc tttgtaaatt ttttaaaaat
     4861 tatgaatcgt atcaagtagt tgtttacatt tgacaaaata ggaactatgc cggcagagtc
     4921 caaccgaaaa ccccctaggt gagataggca gtaactgggc ctgctgtctg cgcctttcgt
     4981 agtgacagat gtggctgtag tgtttaggtg tttggtcttg cttcttgtat ggcatttttc
     5041 aataaacttt aaaaaaaaaa aaaaaa
//
